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Biology
Imaging
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Imaging
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Adjust the required power level with the control knob (default should be 10 to 11 oclock ) the light indicator from the operation mode should stay in the green rangenot far to the red range)
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Lsm 710 Upright Tutorial
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System Start Up
Once you have entered your login, you will see the screen fill with several icons. The software to control the confocal microscope is called ZEN 2009. To start it double click on it. Click Start System .
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Averaging improves the image by increasing the signal to noise ratio. Frame averaging is preferred for fixed samples as it reduces photo-bleaching. Line average is preferred for moving/living specimens.
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Now you are ready to find your sample with the microscope through the ocular.
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Lsm 700 Upright Tutorial
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0.44 NA 1.5 n NA
XY- plane resel = 157 nm Axial resolution = 580nm With Nyquist criterion the ideal voxel is 78.5 nm in XY and 290nm in Z
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Resolution
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2.Increase the Averaging number (2 to 16) using Mean as method. 3.Decrease the Pmt Value (800-600..or less)
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Gray Levels
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Gray Levels
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- - - -
Simultaneous scanning of single or double labelling: Advantage: faster image acquisition Disavantage : Eventual cross-talk between channel Sequentiel scanning of double, triple or forth labelling . Advantage : Only one detector and one laser are switched on at any one time. This reduces cross-talk. Disadvantage : slower image acquisition.
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