Escolar Documentos
Profissional Documentos
Cultura Documentos
> Home
> About Us
> Contact us
I will list all the general procedures that I ever did over here. My colleagues told me that organic synthesis is another way of cooking. After so many year of "cooking", I generalized some common "recipes" for common reactions. Hopefully it will help you, the process of organizing this "recipes" definitely helped me. Reaction set up: The chemicals that you take out from refrigerator or freezer should be keep closed for a couple hours until the temperature of the chemical you want reach to room temperature. A couple hours' waiting might save you several days suffering, especially you are doing water sensitive reactions. The concentration of the reaction sometimes is critical, even though most time does not make huge difference. When your reaction is intra molecular reaction, you should keep your reaction very diluted to prevent the inter molecular side reaction. Larger stir bar is highly necessary for two phase reactions, like Schotten-Baumann acylation or Suzuki coupling (two phases). Because you need to stir the hell of it to have good yield. For large scale, or mixture with high viscosity, or semi-solid reactions, needless to say mechanical stirring is needed. I added low boiling point solvent (heating) to my reaction once to help the agitation. Acylations EDCI (DCC) /DMAP (HOBT) coupling. The carboxylic acid (1 equiv.), amine (1 equiv.), EDCI or DCC ( 1.1 equiv.), and DMAP or HOBT (0.3 equiv.) were dissloved into methylene chloride. The resulting mixture was stirred at room temperature with N2 inlet for 12 hours. The resulting mixture was poured into brine. The organic layer was separated, dried, and concentrated under vacuo. The resulting residue was separated by silica gel column. Weinreb amide formation. To a solution of amine in toluene or DCE was added AlMe3. The mixture was stirred for 20 min at room temperature. The ester solution of toluene or DCE was added to the mixture. The resulting mixture was heated to 80oC for 6 hours. The resulting mixture was diluted with ethyl acetate and washed with brine. The organic layer was dried and concentrated. The residue was purified by silica gel column. Schotten-Baumann acylation. The amine (1 equiv.) was dissolved into methylene chloride . 2 M NaHCO3 aqueous solution was added into the solution. The mixture was stirred vigorously, and then acid chloride (1.5 equiv.) in methylene chloride was added into the mixture slowly. The mixture was stirred for 2 hours. The organic layer was separated, and washed with Na2CO3 aqueous solution three times. The resulting organic layer was dried, and concentrated. The resulting residue was separated by silica gel column. Schotten-Baumann reaction is very good for the acylation of unreactive amine. Weak bases, like RCOOK also could catalyze the acylation between acid chloride and unreactive amine. Alkylations 0.1 to 0.2 Equiv. of KI could promote your reaction, when your alkylation reagent is not active enough. It is called Finkelstein reaction. The mono-alkylation of betaketoester or malonate ester. The starting material, beta-ketoester or malonate ester, (1 equiv.) was dissolved into DMF with Li2CO3 (4equiv.). MeI, or EtI, or BnBr (2 equiv.) was added into the mixture. The resulting mixture was heated to 65oC for 12 hours. GS-MS was used to check the reaction process. The reaction mixture was poured into brine, and ethyl was used to extract the product from
1 of 28
04/09/13 12:09 AM
aqueous layer. The organic layer was dried and concentrated by vacuo. The resulting mixture was easily purified by silica gel column to afford high yield mono-alkylation product. Sometimes LiH is used instead of Li2CO3. The alkylation of substituted malonate or ethyl cyanoacetate. The starting material (1 equiv.) was dissolved into DMF, and RBr (1.5 equiv.) was added into the mixture. K2CO3 (15 equiv.) was added into the resulting mixture in portion. The resulting mixture was stirred at 80oC for 6 hours. The resulting mixture was diluted by ethyl acetate, and washed with brine. The ethyl acetate solution was dried and concentrated. The resulting residue was separated by silica gel column. Borch reductive amination or alkylation. The amine (1equiv.) and ketone or aldehyde (1.5 equiv.) were dissolved into CH2Cl2 or dichloroethane. The mixture was stirred for 3 hours at 60oC. The resulting mixture was cooled to room temperature, and then acetic acid (2.5 equiv.) and NaBH3CN or NaBH(OAc)3 (3equiv.) was added into the mixture. The resulting mixture was stirred for another 8 hours. Ethyl acetate was used to dilute the mixture, and brine was sued to wash the organic layer. The organic layer was dried and concentrated . The resulting residue was purified by silica gel column to afford 70% product. Click here to learn more. This reaction is similar to Eschweiler-Clarke reaction, in which formaldehyde is used, and formic acid is used as hydrogen donor. The alkylation of amine with alkyl bromide. The amine (1 equiv.) and alkyl bromide (1.1 equiv.) were dissolved into DMF with TEA (3 equiv.). The mixture was stirred for 8 hours at 80oC. The resulting mixture was diluted with ethyl acetate, and washed with brine. The organic layer was dried and concentrated under vacuo. The resulting residue was separated by silica gel column. The common side reaction was over alkylation, or elimination of alkyl bromide. Condensations Knoevenagel condensation 1. The ketone (1 equiv.) or aldehyde, ethyl cyanoacetate (2 equiv.), ammonium acetate (5 equiv.), and acetic acid (10 equiv.) were mixed into toluene. The resulting mixture was heated to reflux for 48 hours. The resulting mixure was diluted with ethyl acetate and washed with brine. The organic layer was dried and concentrated. The resulting residue was purified by silica gel column. Knoevenagel condensation 2. The ketone (1 equiv.) or aldehyde, diethyl malonate(2 equiv.) were dissolved into THF. TiCl4 (2 equiv.) was added into the mixture dropwise with a N2 inlet at 0oC. Pyridine (4 equiv.) was added into the mixture also at 0oC. The resulting mixture was stirred for 12 hours at room temperature. The resulting mixture was filtered, the filtrate was diluted with ethyl acetate and washed with brine. The organic layer was dried and concentrated. The resulting residue was purified by silica gel column. Mukaiyama aldole condensation. The starting material, ketone (1 equiv.), was dissolved in THF, and cooled to -78oC. TiCl4 (2 equiv.) was added into the mixture dropwise. TMS enol or enolate (1.3 equiv.) in THF was added into the mixture dropwise. The resulting mixture was stirred for 4 hour at room temperature, and then 50oC for 2 hours. The resulting mixture was diluted with ethyl acetate, and washed with brine. The ethyl acetate solution was dried and concentrated. The residue was purified by silica gel column. Reformatsky reaction. The starting materials, ketone (1equiv.), alpha-bromoester (1.5 equiv.), and Zinc (2 equiv.) activated by TMSCl, were dissolved into THF. The resulting mixture was heated to 60oC for 12 hours with a N2 inlet. The resulting mixture was filtered. To the filtrate, 2 N HCl was added and stirred for another 2 hours. The resulting mixture was diluted with ethyl acetate and washed with brine. The organic layer was dried, concentrated, and the residue was purified by silica gel column to yield alpha, beta unsaturated ester. Decarboxylations The malonate derivative (1 equiv.) was dissolved into methanol, and 2 M KOH (5 equiv.) was added into the solution. The resulting mixture was acidified using HCl to pH 2. The resulting mixture was stirred for
2 of 28
04/09/13 12:09 AM
another 2 hours. Ethyl acetate was used to extract the product from aqueous layer. The organic layer was dried, concentrated, and the residue was purified by silica gel column to afford a mono acid. The diethyl malonate (1equiv.) was dissolved into DMSO, and LiCl (15 equiv.) was also added into the mixture. The resulting mixture was heated to 190oC with N2 inlet for 4 hours. The resulting mixture was cooled to room temperature and diluted with ethyl acetate. After filtering, the filtrate was washed with brine. The organic layer was dried and concentrated. The resulting residue was purified by silica gel column to afford an ethyl ester. Reductive decyano group. To a THF solution of ethyl cyano acetate derivative (1equiv.) with HMPA (10%) was added SmI2 (2 equiv.). The resulting mixture was stirred for 8 hours at room temperature. The resulting mixture was filtered. The filtrate was diluted with ethyl acetate and washed with brine. The organic layer was dried and concentrated. The residue was purified by silica gel column. Halogenations Chloronation of alcohol. The starting material, primary alcohol, (1equiv.) was dissolved into THF with Bu4NCl (0.2 equiv.). Thionyl chloride was added into the solution very slowly under 0oC. The mixture was stirred for 3 hours at room temperature. 2 M K2CO3 aqueous solution was added into the mixture to adjust the pH to 8. Ethyl acetate was used to extract the product from aqueous solution. The organic layer was dried and concentrated under vacuo. The residue was separated by silica gel column to afford 85% yield. Acid chloride from acid. The acid (1 equiv.) was dissolved into methylene chloride with 0.5 mL of DMF. Thionyl chloride or oxalyl chloride (2 equiv.) was added into the solution slowly. The mixture was stirred 1 hour at room temperature, and then heated to 70oC for another 4 hours. The resulting mixture was concentrated. The residue was used as it without further purification. On certain extremely condition thionyl chloride could be used as solvent. Chloronation of aromatic ring from hydroxyl group. The starting material, hydroxyl-heterocycle, (1 equiv.) was added into POCl3 (30 equiv.). TEA, or dimethylaniline, or pyridine (20 equiv.) was also added into the mixture slowly. The resulting mixture was heated to 60oC for 4 hours. The resulting mixture was added into ice-water dropwise with strong stirring. The precipitate was collected, and crystallized to afford 60% product. Bromination of alcohol. The starting material, primary alcohol, (1 equiv.) and PPh3 (1.5 equiv.) were dissolved into THF at 0oC. Bromine (1.2 equiv.) was added into the mixture slowly. You would notice the color change. The resulting mixture was stirred for another 3 hours at room temperature. After filtering, the filtrate was concentrated under vacuo. the resulting residue was good enough for next reaction. Radical bromination of benzylic position. The alkyl substituted aromatic system was dissolved into CCl4 with AIBN (0.2 equiv.) and NBS (1.5 equiv.). The mixture was heated to 65oC for 4 hours. The resulting mixture was diluted with ethyl acetate, and washed with brine. The organic layer was dried and concentrated. The residue was separated by silica gel column to give product with 70% of yield. CF3Ph could be used to replace CCl4 for higher temperature. Bromination through electrophilic substitution mechanism. The aromatic compound was dissolved into acetic acid, and Br2 was added into the solution dropwise. The resulting mixture was heated to 70oC and stirred for 7 hours. The resulting mixture was diluted with ethyl acetate and washed with brine. The organic layer was dried and concentrated. The residue was purified by silica gel column. NBS, dibromomethylhydatoin, and Br2/Fe could be used as bromo sourse. Polar solvent THF. dioxane are highly necessary for the reaction. Bromination of aromatic ring through Sandmeyer reaction. To a dark solution of CuBr2 (3 equiv.) in CH3CN in a two necked bottle, was added t-butyl nitrite (1.3 equiv.) slowly. The resulting solution was heated to 60oC for 20 min. to above solution, was added CH3CN solution of aniline type of starting material (1 equiv.) dropwise. The resulting mixture was heated to 60oC for 3 hours. The resulting mixture was transferred to 1 M NaOH aqueous solution, and extracted with ethyl acetate. The organic layer was dried, and concentrated. The residue was separated by silica gel column.
3 of 28
04/09/13 12:09 AM
Iodonation of aromatic ring. The electronic rich aromatic ring (1 equiv.), ZnCl2 (3 equiv.), and BnNMe3(ICl2) (1.5 equiv.) were dissolved into acetic acid. The mixture was stirred at room temperature for 48 hours. The resulting mixture was poured into ice water, and extracted with ethyl acetate. The organic layer was washed with brine, dried and concentrated under vacuo. The resulting residue was separated by silica gel column. Iodonation by NIS. To a suspension of starting material (1 equiv.) in dry CH3CN was added n-iodosuccinimide (1.1 equiv.), and the mixture was stirred at room temperature fror 1 hour. The reaction mixture was heated at reflux for 10 min and then cooled to room temperature. Methylene chloride was added into the reaction mixture and the organic phase was washed successively with NaOH 10%, sodium thiosulfate, and brine. The resulting organic phase was driedover magnisum sulfate, filtered, and concentrated to dryness under reduced pressure. The resulting residue was separated by silica gel column. Swarts fluoroalkane synthesis. A mixture of trichlorotoluene (1 equiv.) and SbF5 (1.1 equiv.) was heated until the reaction started. After completion of the reaction, the mixture was washed with HCl and dried. The residue was separated accordingly. Hydrolysis The ester (1equiv.) was dissolved into methanol, and KOH, or LiOH, or NaOH (20 equiv.) aqueous solution was added into the solution. THF was used to make reaction homogenous. The mixture was stirred for 40 min. at room temperature. Acetic acid was used to adjust the pH to 5. Ethyl acetate was used to extract the product from aqueous layer. The organic layer was dried and concentrated. The residue was crystallized to afford 90% product. Hydrogenations Parr Shaker. The starting material (1 equiv.) in ethanol or methanol with 5-10% of Pd-C [Pd(OH)2 on carbon, Pearlman reagent, is a good choice also] was put onto parr shaker. The regular pressure is from 20 to 60 psi. You should pay a little extra attention on mechanic problems besides flushing the bottle with H2. Filter the mixture, Pd catalyst was collected into special container with water, and filtrate was concentrated. The residue was separated by silica gel column. Transfer hydrogenation. The starting material (1 equiv.) was dissolved into methanol with HCO2NH4 or NH2NH2 (20 equiv.). Pd-C 5% was added into the mixture. The resulting mixture was heated to 80oC with N2 inlet for several hours until TLC showed no starting material left. Work up is similar to that of Parr shaker. Ionic hydrogenation (Kursanov-Parnes reaction). The CH2Cl2 solution of starting material, ArCOAr' (1 equiv.), was put into ice-NaCl bathe. CF3SO3H (1 equiv.) and triethylsilane (1equiv.) was added into the mixture sequentially. The mixture was stirred for 20 min, and another 2 equiv. of CF3SO3H and triethylsilane was added. The resulting mixture was stirred at room temperature for 12 hours. The resulting mixture was poured into ice-water with sodium carbonate. The product was extracted from aqueous layer. The organic layer was dried and concentrated under vacuo. The residue was separated by silica gel column to afford product (70%). This reaction is very good for base sensitive compound reduction. Oxidations Dess-Martin oxidation. The primary alcohol (1 equiv.) was dissolved into methylene chloride, and then Dess-Martin periodinane (1.1 equiv.) was added into the solution. The resulting mixture was stirred at room temperature for 12 hours. Saturated NaHCO3 aqueous solution with sodium thiosulfate was then added to the mixture. The resulting mixture was stirred at 40oC for 30 min. The organic layer was washed with brine, separated, dried, and concentrated. The resulting residue was purified by silica gel column. Sodium thiosulfate was used to reduce extra of Dess-Martin reagent. Swern oxidation. The alcohol (1 equiv.) was dissolved into THF with DMSO (1.5 equiv.). The resulting
4 of 28
04/09/13 12:09 AM
mixture was cooled to -78oC. Oxalyl chloride (1.5 equiv.) was added into the mixture. The mixture was stirred for 30 min at -78oC. TEA (2 equiv.) was added into the mixture dropwise. The resulting mixture was stirred 1 hour at -78oC, and 2 hours at room temperature. The resulting mixture was diluted with ethyl acetate and washed with brine. The organic layer was dried and concentrated. The resulting residue was separated by silica gel column. Rearrangements Claisen rearrangement. The allyl phenol in a flask with N2 inlet was heated to 200oC for 16 hours. TLC showed trace amount of starting material. The flask was cooled to room temperature. The resulting residue was purified by silica gel column. Dimethylaniline could be used as solvent in this reaction. Reductions LAH ester reduction: The starting material (ester or amide1 equiv.) was dissolved into THF, and LAH (solid or in THF) was added into the solution at -78oC. The mixture was stirred for 1 hour at -78oC, and then the mixtrue was stirred for another 2 hours at room temperature. KOH 10% in water or Rochelle's salt was added into the mixture slowly. Ethyl acetate was used to extract the product (alcohol from ester or amine from amide) from aqueous solution. The organic layer was dried, concentrated. The resulting residue was separated by silica gel column to give 70% yield. NaBH4 ketone reduction. The staring material, ketone, was dissolved into methanol, and NaBH4 (2 equiv.) was added into the mixture by portion. The mixture was stirred for 2 hours. The resulting mixture was diluted by ethyl acetate, and washed with brine. The organic layer was dried and concentrated. The residue was separated by silica gel column. DIBAL reduction ester to aldehyde. The ester (1 equiv.) was dissolved into CH2Cl2. The temperature of the solution was coole to -78oC. DIBAL in THF (1.2 equiv.) was added into the solution dropwise with a N2 inlet. The resulting mixture was stirred at -78oC for 1 hour. Methanol was added into the mixture slowly, and then brine was added into the mixture. The organic layer was separated, dried, and concentrated. The residue was purified by silica gel column. Reactions on heterocycle ring The choice of base or acid to do the cyclization. If the leaving group of cyclization is hydroxyl or amine group, acid have to be used, because only protonated amine or hydroxyl group could become leaving group. If the leaving group is alcohol, base has to be used. If nucleophile has to be activated, using base, otherwise, using acid. Substitutions Azide substitution. Alkyl bromide (1 equiv.) and sodium azide were dissolved into DMF. The mixture was heated up to 80oC for 12 hours. To the resulting mixture, were added PPh3 (3 equiv.) and H2O (30 equiv.) at room temperature. The resulting mixture was stirred at room temperature for 12 hours. A primary amine (75%) was made after work up and purification. The second part of this reaction is called Staudinger azide reduction. Aromatic substitution 1. 4-Hydroxylcoumarin (1 equiv.) and aniline (50 equiv.) were mixed together with a N2 inlet. The mixture was stirred for 2 hours under reflux. The resulting mixture was dissolved into methanol. 0.1 M NaOH aqueous solution was added into the methanol solution slowly. The yellow precipitate was collected, and washed by water. Halogen substitution from an electronic deficient aromatic ring. The halo-aromatic ring (1 equiv.), amine or alcohol (2 equiv.), and base (TEA, or diisopropylethylamine 2 equiv.) were mixed into DMF. The mixture was stirred at 95oC for 8 hours with a N2 inlet. Ethyl acetate was used to dilute the resulting mixture. The
5 of 28
04/09/13 12:09 AM
resulting mixture was washed with brine. The organic layer was dried, concentrated, and the residue was purified by silica gel column. Floride substitution from an electronic rich aromatic ring. Substitution of MsOR. Step 1, making MsOR. The alcohol (1 equiv.) was dissolved into THF with TEA( 1.5 equiv.). MsCl (1.2 equiv.) was added into the solution slowly at 0oC. The mixture was stirred for 3 hours. The resulting mixture was diluted with ethyl acetate, and washed with brine. The organic solution was dried, and concentrated. The resulting residue was used in next step without further purification. Step 2, substitution. The amine (1.3 equiv.) was added into the acetonitrile solution of the product of step 1. TEA (1.5 equiv.) was also added into the mixture. The resulting mixture was stirred at room temperature for 12 hours. The resulting mixture was diluted by ethyl acetate, and washed with brine. The organic solution was dried, and concentrated. The resulting residue was separated by silica gel column. The common side reaction is elimination. Mitsunobu reaction. The phenol (1.2 equiv.), primary alcohol (1.0 equiv.), Dead or ADDP (1.5 equiv.), and PPh3 (1.5 equiv.) were dissolved into THF with N2 inlet. The resulting mixture was stirred for 3 hours, and precipitate was formed. The mixture was stirred for another 2 hours. The mixture was filtered, and filtrate was directly loaded to silica gel column. Precipitate (OPPh3) formation is a good sign for Mitsunobu reaction. And the common side reaction is elimination. Intramolecular Mitsunobu reaction is much faster than inter molecular Mitsunobo. Sometimes, high concentration helps this reaction. NMR & MS NMR, chemical shifts of deuterated solvents could come from proton in trace of amount of non-deuterated solvent, proton exchanged with trace amount of water in the solvent if you have a polar solvent, and water proton when water make hydrogen bond with the solvent. These chemical shifts will change with temperature and in some case they change with percentage of water in the solvent. The chemical shifts listed in my table are obtained at room temperature (22oC). EDAC/HOBT Coupling.
J. Med. Chem. 1998, 41, 4288-4300 A soulution of 3.42 g (15.00 mmol) 2-phenyl-4-hydroymethylbenzoic acid in 30 mL of DMF was treated sequentially with 2.20 g (16.30 mmol) of HOBT, 3.12 g (16.30 mmol) of EDAC, and 3.90 g (19.50 mmol) of L-methionine methyl ester hydrochloride. The suspension was stirred for 15 min, 4.00 mL (27.00 mmol) of TEA was added, and the mixture stirred for 24 hours. The reaction was quenched by pouring into 300 mL of ethyl acetate and extracting with 3 portions of 1 N aqueous HCl, 2 portion of water, 1 portion of brine, 1 portion of saturated aqueous NaHCO3, and a portion of brine. The solution was then dried over Na2SO4, filter, and concentrated. The residue was purified by column chromatography on silica gel (120 g, 70:30 ethyl acetate/hexanes) to provide 4.4 g (79%) of product as an oil that solidified upon standing. A Grignard reaction.
6 of 28
04/09/13 12:09 AM
Org. Process Res. Dev., 11 (2), 206 -209, 2007. Synthetic Procedure for 1-(1H-Imidazol-4(5)-yl)-2-methylpropan-1-one (9). A solution of 4(5)-cyanoimidazole (42.7 g, 0.458 mol) in THF (500 mL) was added dropwise over 30 min to a solution (1.4 L, 1.47 mol) of 1.1 M isopropylmagnesium bromide in THF below 10 oC under a nitrogen atmosphere. The mixture was stirred at room temperature for 3 h. Water (430 mL) and 10% aqueous sulfuric acid solution (860 mL) were added dropwise, and the mixture was stirred at 30 min and titrated to pH 8 with 30% aqueous sodium hydroxide solution. After the organic layer was separated, the aqueous layer was extracted with ethyl acetate (300 mL 2). The organic layers were combined, and the mixture was washed with aqueous sodium hydrogen carbonate and brine and concentrated under reduced pressure. The crystals formed were collected by filtration and washed with isopropyl ether (300 mL). The crystals were dried in vacuo (40 oC) to give 1-(1H-imidazol-4(5)-yl)-2methylpropan-1-one (51.9 g, yield 82%).
Aldol Condensation:
Org. Process Res. Dev., 11 (1), 13 -18, 2007. 7-[3-(4-Fluorophenyl)-1-(1-methylethyl)-1H-indol-2-yl]-5-hydroxy-3-oxo-6-heptenoic acid-1,1dimethylethylester. In a suitable reactor charged with nitrogen, sodium hydride (60% in paraffin oil, 6.0 g, 0.15 mol) was mixed with THF (120 mL) to form a suspension at 20-25 oC. tert-Butyl acetoacetate (23.7 g, 0.15 mol) was added within 30 min maintaining the temperature at 20-25 oC and the mixture was further stirred for 15 min. After cooling to 0 oC, butyllithium (1.6 M in hexane, 97.5 mL, 0.15 mol) was added within 60 min, maintaining the temperature at 0 oC. The reaction mixture was stirred at this temperature for a further 30-45 min. A solution of starting material (38.5 g, 0.125 mol) dissolved in THF (100 mL) was added to the reaction mixture, maintaining the temperature at 0 oC. After 60 min stirring at 0 C, the reaction mixture was quenched with acetic acid (22.6 g, 0.38 mol) and then with water (43.5 mL). The reaction mixture was extracted twice with saturated sodium chloride solution (100 mL) and once with water (50 mL). After removal of the solvents at 30-50 oC and 30 mbar pressure, the evaporation residue containing 57.5 g of product (98.8%) was diluted in THF (75 mL) and kept at 0-5 oC (storage solution of product). Knoevenagel Condensation.
7 of 28
04/09/13 12:09 AM
Org. Process Res. Dev., 11 (2), 190 -199, 2007. Preparation of 5-[4-(2-{[6-(4-Methoxyphenoxy)pyrimidin4-yl]methylamino}ethoxy)benzylidene]thiazolidine-2,4-dione. To a dry, nitrogen-purged 70-L reactor (Buchi) with a mechanically stirred solution of starting material aldehyde (1.8 kg, 4.8 mol) in 30 L of toluene were successively added 2,4-thiazolidinedione (0.48 kg, 4.8 mol), acetic acid (70 mL, 1.44 mol), and piperidine (0.12 L, 1.4 mol), and then the reaction mixture was stirred at reflux temperature for 5 h with a Dean-Stark water trap (the amount of condensed water was about 86 mL). The disappearance of the starting material was detected by monitoring HPLC (HPLC retention time; starting 7: 12.5 min, product 10: 13.5 min). Once the reaction was completed, the mixture was cooled to room temperature, and the precipitates formed were collected by filtration and washed twice with fresh anhydrous toluene. Reformatsky Reaction.
Organic Process Research & Development 2005, 9, 216-218 The trick of Reformatsky reaction is that the Zn metal must be activated in order to let the Zn react with haloester smoothly. The Zn metal could be activated by heating it with TMSCl, I2, MeMgBr, 1, 2-dibromoethane, CuCl and CuI. Procedure: CuCl (1.2 kg, 12 mol, 0.2 equiv) was charged into a 250 L alloy 59 vessel. THF (40.0 kg) was added at 20oC. To this solution, Zn powder (US-Zinc, 7.1 kg, 106 mol, 1.7 equiv) was added, followed again by THF (48.8 kg). Concentrated sulfuric acid (1.1 kg, 12 mol, 0.2 equiv) was added, and the mixture was heated to 45 oC. When the target temperature was reached, a solution of indanone (9.8 kg, 60 mol) in THF (88 kg) was added. The latter solution was prepared prior to the reaction and tempered at 45oC. After addition of the indanone solution, bromoester (13.4 kg, 73 mol, 1.2 equiv) was dosed over approximately 15 min. The pump and pipes were rinsed with THF (2 kg). The reaction mixture was stirred with a jacket temperature of 45oC for 1.5 h and subsequently cooled to 20oC. Diluted sulfuric acid (c =20%, 33.3 kg) was added, and the mixture was stirred at 20oC for 10 h. The mixture was transfered via a filter nutsche to 650 L alloy 59 vessel. The first vessel, the filter nutsche, and all pipes were rinsed with toluene (64.8 kg). The mixture was concentrated in vacuo (150 mbar) by distilling off THF (90 kg). Water (3 X 30 kg) was added, and the layers were separated. The aqueous layers were disposed of. THF and toluene (97 kg) were distilled off in vacuo (150 mbar), resulting in a solution of product in toluene (32.7 kg, 41.9 wt % of product), corresponding to an assay-corrected yield of 92% (13.70 kg of product). Wittig Horner reaction
8 of 28
04/09/13 12:09 AM
Org. Process Res. Dev., 11 (2), 200 -205, 2007 (5E,7E,22E)-(1S,3R)-1,3-Bis(tert-butyldimethylsilyloxy)-24-oxo-25-(5-butyloxazol-2-yl)-26,27-cyclo9,10-secocholesta-5,7,10(19),22-tetraen . To 4.84 g of sodium hydride (121 mmol, 60% of a dispersion in paraffine) in 100 mL of THF at 0 oC were added slowly 42 g (133 mmol) of starting material aldehyde dissolved in 250 mL of THF. The solution was stirred for 1 h at rt, and then 65.9 g (115 mmol) of (5E,7E)-(1S,3R)-1,3bis(tert-butyldimethylsilyloxy)-9,10-secopregna-5,7,10(19)-triene-20-carbaldehyde dissolved in 400 mL of THF were added. The solution was stirred 1 h at 20 C and 19 h at 50 C. The reaction was monitored by thin layer chromatography (TLC) on SiO2 plates (eluent ethyl acetate/hexane 1:9 v/v), Rf = 0.15 (product), Rf = 0.53 (educt 4). 6 g (100 mmol) of acetic acid in 50 mL of THF were added for workup followed by 1 L of water, with citric acid used to adjust pH to 7. The product was extracted twice with 500 mL of methyl tert-butylether and evaporated to dryness. The crude material was purified by chromatography on silica gel by elution with hexanes and an increasing gradient of ethyl acetate (0-15%). The evaporation of the fractions gave 79.5 g of product in 90% yield. Oxane oxidation of the sulfide
9 of 28
04/09/13 12:09 AM
with a 2:1 (v/v) mixture of acetone/water (11.2 kg) and then with water (50 kg). The wet product was dried under vacuum at 45 C. The dry weight of product was 2.66 kg, and the potency adjusted yield was 85% (sulfide is 97% potent).
Swern Oxidation
1-(2-Oxo-hexylcarbamoyl)cyclopropanecarboxylic Acid Methyl Ester: A solution of 35.8 mL (0.5 mol) of DMSO in 100 mL of dichloromethane was cooled to -40 oC, and 19.8 mL (0.23 mol) of oxalyl chloride in 198 mL of dichloromethane (precooled to -40 oC) were slowly added. After 10 min of stirring, a solution of 51.6 g (0.18 mol) 1-(2-hydroxy-hexylcarbamoyl)cyclopropanecarboxylic acid methyl ester (crude, content 83%, dissolved in 198 mL of dichloromethane) was slowly added over a period of 40 min. The reaction was stirred for 30 min at -40 oC. Then 69.8 mL (0.5 mol) of triethylamine were added followed by 300 mL of water. Extraction with dichloromethane and evaporation of the solvent yielded 44.72 g (100% yield) of crude product as an oil. Hydrogenation general procedure
10 of 28
04/09/13 12:09 AM
magnetic stirring bar, and was purged with nitrogen prior to the sealing. Subsequently the reactors were loaded in an autosampler and reacted at 100 C from 10 to 300 min. Initial investigation of the reaction mixtures left at room temperature for 24 h showed no conversion of the reactants.
palladium-catalyzed cyanation
Negishi Coupling
11 of 28
04/09/13 12:09 AM
Org. Process Res. Dev., 11 (2), 237 -240, 2007 2-Chloro-5-(pyridin-2-yl) Pyrimidine. A 2-L flask was charged with tetrahydrofuran (130 mL) and purged with nitrogen. The mixture was cooled to -70 oC, and a solution of hexyllithium in hexanes (175 mL 2.5molar, 0.436 mol) was added dropwise at such a rate that the inner temperature remained below -50 oC. On completion of the addition the mixture was cooled to -65 oC, and 2-bromopyridine (65.7 g, 0.416 mol) was added at such a rate that the inner temperature remained below -65 oC. Subsequently, a solution of ZnCl2 (57.8 g, 0.416 mol) in tetrahydrofuran (268 mL) was added below -55 oC. Upon completion of the addition, the mixture was allowed to warm to approx 22 C. After stirring for another 2 h, Pd(PPh3)4 (4.8 g, 0.004 mol) was added. Subsequently, a solution of 5-iodo-2-chloropyrimidine (50 g, 0.2 mol) in tetrahydrofuran (160 mL) was added at such a rate that the temperature remained below 30 oC. After stirring for another hour, a 0.39 M aqueous solution of EDTA3Na (1 L) and dichloromethane (100 mL) were added, and the mixture was stirred vigorously for 15 min. The layers were separated, and from the organic layer the solvents were evaporated under reduced pressure, leading to a black oily residue. The residue was dissolved in dichloromethane (300 mL), and to the solution were added Kieselgel 60 (66 g) and thiol-modified silica (6.1 g).The mixture was stirred for 20 min and filtered. The solid collected was washed with dichloromethane (3 aliquots of 230 mL). From the filtrate the solvents were evaporated until the residual volume reached approx 350 mL. After cooling to approx 22 oC the residue was washed with a 2 N hydrochloric acid solution (2 aliquots of 350 mL). The combined aqueous layers were treated with Norit A Supra (1.4 g). After filtration the filtrate was neutralized with aqueous ammonia (pH 6-7). The resulting precipitate was collected by filtration and dried in vacuo at 40 oC, yielding 25.6 g (67%) of the title compound. Sonogashira coupling reaction
Org. Process Res. Dev., 11 (2), 246 -250, 2007 To a 1 L round-bottom flask were added THF (250 mL), 4-(butyn-3-yl)benzonitrile (49.38 g, 0.318 mol), TEA (250 mL, 1:1 TEA/THF), bromopyridine (33.3 mL, 0.35 mol), Pd (PPh3)2Cl2 (4.47 g, 6.0 mmol), and CuI (1.21 g, 6.0 mmol). The reaction mixture was stirred at 60 oC for 2 h. The reaction was then cooled to room temperature and quenched with water (100 mL) and ethyl acetate (100 mL). The organic layer was separated and washed with water (50 mL) and brine (30 mL), dried over sodium sulfate, and concentrated in vacuo to give a brown solid, which was subsequently washed with heptane (2 50 mL) to afford 4-(4-(pyridin-2-yl)butyn-3-yl)benzonitrile as an off-white solid (58.73 g, 79.6%) with a purity of 98.4% (HPLC, 230 nm). Reductive carbonylation
12 of 28
04/09/13 12:09 AM
Org. Process Res. Dev., 11 (1), 39 -43, 2007. General Procedure for the Reductive Carbonylation of 4-Bromotoluene (Method A). To 4-bromotoluene (0.616 g, 3.6 10-3 mol), [PdCl2(dppp)] (0.053 g, 9 10-5 mol), and Na2CO3 (0.381 g, 3.6 10-3 mol) were added DMF (5 mL), mesitylene (0.25 mL, 1.8 10-3 mol) as reference, and Et3SiH (1.166 mL, 7.2 10-3 mol). Once sealed inside the reactor the system was purged with CO several times. The temperature was raised to 90 C, and the reactor was charged with 3 bar of CO. The reaction was stirred under these conditions for 18 h after which the reactor was allowed to cool. The reaction was filtered, and the filtrate was analysed by GC-MS to determine the conversion and yield of aldehyde. General Procedure for the Reductive Carbonylation of 4-Bromobenzonitrile (Method B). To 4-bromobenzonitrile (0.655 g, 3.6 10-3 mol) and [PdCl2(dtbpf)] (0.065 g, 9 10-5 mol) were added THF (5 mL), mesitylene (0.25 mL, 1.8 10-3 mol) as reference, Et3N (0.504 mL, 3.6 10-3 mol), and Et3SiH (1.166 mL, 7.2 10-3 mol). Once sealed inside the reactor the system was purged with CO several times. The temperature was raised to 90 C, and the reactor was charged with 3 bar of CO. The reaction was stirred under these conditions for 18 h after which the reactor was allowed to cool. The reaction was filtered, and the filtrate was analysed by GC-MS to determine the conversion and yield of aldehyde. General Procedure for the Reductive Carbonylation of 4-Bromoanisole (Method C). To [Pd(PtBu3)2] (0.046 g, 9 10-5 mol) were added dioxane (5 mL), mesitylene (0.25 mL, 1.8 10-3 mol) as reference, 4-bromoanisole (0.451 mL, 3.6 10-3 mol), Et(iPr)2N (0.658 mL, 3.6 10-3 mol), and Et3SiH (1.166 mL, 7.2 10-3 mol). Once sealed inside the reactor the system was purged with CO several times. The temperature was raised to 120 C, and the reactor was charged with 3 bar of CO. The reaction was stirred under these conditions for 18 h, after which the
13 of 28
04/09/13 12:09 AM
reactor was allowed to cool. The reaction was filtered, and the filtrate was analysed by GC-MS to determine the conversion and yield of aldehyde.
Chloronation Reaction
Org. Process Res. Dev., 11 (2), 237 -240, 2007 2-Chloro-5-iodo-pyrimidine. A 3-L flask was charged successively with 2-hyroxy-5-iodo-pyrimidine (100 g, 0.45 mol), acetonitrile (540 mL), and phosphorous oxychloride (82.9 g, 0.54 mol). Diisopropylethylamine (29.1 g, 0.23 mol) was added dropwise. Upon addition, the mixture was heated to reflux and kept under the same heating conditions for 20 h. After the mixture was cooled to 40 C, water (900 mL) was added over 20 min. The formed black precipitate was extracted with ethyl acetate (1130 mL). The extract was washed with an aqueous solution of sodium sulfite (150 g in 570 mL). The extract was transferred into a 2-L flask, and water (270 mL) was added. The mixture was heated to reflux, and about 600 mL of solvent was distilled off, and another portion of water (540 mL) was added. Solvent evaporation was continued until the inner temperature reached 85 oC. The mixture was cooled to approx 22 oC and filtered. The precipitate washed with water (100 mL) and dried at 40 oC in vacuo yielding 78.4 g (72%) of the title compound
Nenitzescu reaction
Org. Process Res. Dev., 11 (2), 241 -245, 2007 1-(4-Fluorophenyl)ethyl 5-Hydroxy-2-methyl-1H-indole-3-carboxylate. A 20-L jacketed vessel was charged with 2-propanol (10 L), acetic acid (700 mL), and 1,4-benzoquinone (850 g, 7.87 mol). The mixture was heated, and reflux was obtained after 1 h (IT 85 oC). A solution of 1-(4-fluorophenyl)ethyl 3-aminobut-2-enoate (1.569 g, 7.04 mol) in 2-propanol (3 L) was added over 25 min, and heating was continued. A sample was taken after 30 min and concentrated, and NMR analysis indicated the reaction was complete. After an additional 10 min, the mixture was cooled to 20 oC over 1 h. The mixture was concentrated, taken up in dichloromethane (8 L), and washed with 10% aqueous potassium carbonate (2 6 L). The aqueous layers were combined and extracted with dichloromethane (2 L). The organic layers were combined, dried with magnesium sulfate (700 g), filtered, and concentrated to approximately 7.5 L total volume. Mannich reaction
14 of 28
04/09/13 12:09 AM
Org. Process Res. Dev., 11 (2), 241 -245, 2007 1-(4-Fluorophenyl)ethyl 4-Dimethylaminomethyl-5-hydroxy-2-methyl-1H-indole-3-carboxylate Hydrochloride. A 20-L jacketed vessel was charged with dichloromethane (7.5 L) and N,N,N',N'tetramethylmethylenediamine (1.25 L, 9.16 mol) and was cooled to IT 0 oC. Acetyl chloride (625 mL, 8.79 mol) was added over 30 min, causing the internal temperature to rise to 20 oC (jacket reaching -25 oC). After 35 min, potassium carbonate (1.31 kg, 9.48 mol) was added (IT 0 oC), the mixture was stirred for 5 min more, and 1-(4-fluorophenyl)ethyl 5-hydroxy-2-methyl-1H-indole-3-carboxylate (7.32 mol in 7.5 L of dichloromethane) was added over 10 min causing the IT to rise to 8 oC. The mixture was warmed to 20 oC over 30 min and stirred at this temperature. The reaction was monitored by 1H NMR using concentrated aliquots. After stirring at 20 oC for 100 min, water (5 L) was added and the mixture stirred vigorously for 10 min and allowed to settle. The layers were separated, and the organic phase was washed with an additional portion of water (5 L). The aqueous layers were combined and extracted with dichloromethane (2.5 L). The organic layers were combined, dried with magnesium sulfate (824 g), filtered, and concentrated. The residue was taken up in ethanol (4.5 L) and transferred to a 10-L round-bottomed vessel with overhead anchor stirring. A solution of HCl in ethanol was prepared by addition of acetyl chloride (657 mL, 9.24 mol) to ethanol (1.5 L) with cooling. This solution was added over 85 min to the Mannich product, using an ice bath to control the IT < 35 oC. Precipitation was observed before the addition was complete. The mixture was stirred at ambient temperature for 2.5 days. The mixture was then cooled in an ice-water bath for 30 min (IT 6 C) before dividing into two portions and filtering. The cakes were washed with ice cold ethanol (2 500 mL each) and dried in a vacuum oven at ambient for 23 h to give 1.375 kg, as a light-purple solid. Indolizine Synthesis
3-(4-Cyanobenzoyl)indolizine. To a solution of 4-acetylbenzonitrile (34 g, 235 mmol) in EtOAc (320 mL) was added Br2 (neat, 11.9 mL, 320 mmol) at room temperature. The reaction mixture was stirred at room temperature for 1 h. After the solvent was removed under reduced pressure, the resulting residue was dissolved in CH3CN (210 mL). To it was added picoline (50 mL, 500 mmol). After the reaction mixture was stirred for 2 h at room temperature and then 0.5 h at 0 C, EtOAc (70 mL) was added to the mixture. The resulting precipitates were collected by filtration and washed with EtOAc to give 2-methyl-1-(4-cyano)phenacylpyridinium bromide (60 g, 88%) which was used directly in the next step. 1H NMR (300 MHz, DMSO-d6) (ppm) 9.05-8.03 (m, 8H), 6.78 (s, 2H), 2.74 (s, 3H). To a stirred suspension of the above picolinium salt (50 g, 120 mmol) in DMF (500 mL) was added DMF-Me2SO4 (400 mL) (pre-prepared in a separate reaction flask by stirring a mixture of equal mole equivalents of DMF and Me2SO4 at 60-80 C for 3 h, then cooled to room temperature). After the addition, the reaction mixture was stirred at room temperature for 15 min. To it was then added Et3N (500 mL) while the inner temperature was kept between 25 and 40 C. After stirring at room temperature for 2 h, the reaction mixture was
15 of 28
04/09/13 12:09 AM
charged with ice water (2000 mL) with stirring which led to the formation of slurry. The precipitates were collected, washed with water, and dried under a vacuum to give 29 g (76%) of 3-(4-cyanobenzoyl)indolizine. Rf 0.3 (10% ethyl acetate in hexanes); mp 156-157 C (recrystallized from ethyl acetate). Shi Epoxidation
The potassium trans-alkenoate solution from the Suzuki cross-coupling was adjusted to pH 10.5 with 25% aqueous H2SO4. The solution was cooled to between -5 to 5 oC and D-Epoxone (4.3 g, 0.017 mol), as a solution in acetonitrile (20 mL) was added. While the mixture was stirred with vigorous agitation, Oxone (45.3 g, 0.074 mol) in water (160 mL) was added to the mixture. The temperature was maintained below 10 oC by control of the Oxone solution charge rate, and the pH was maintained at 10.0-11.0 by the addition of 20% KOH as necessary. After the addition was complete, the mixture was held at 10-15 oC for 1 h. The mixture was adjusted to pH 2 with 25% H2SO4. Toluene (233 mL) was added, the mixture was heated to 75-80 oC, and the layers were separated. The organic layer was filtered and concentrated until the pot volume was approximately 100 mL. The solution was cooled to 20-30 oC, and heptane (67 mL) was added over approximately 1 h to crystallise the product. The solids were filtered, washed with cold heptane (~15 mL), and dried at 35-40 oC to afford a 55% yield of hydroxylactone in 86%.
3H-pyrrolo[2,3-d]pyrimidin-4(7H)-one Synthesis
Representative Procedure To a solution of LiOH (17.25 g, 1.5 equiv) in anhydrous methanol (600 mL) was added cyanoacetamide (63 g, 1.5 equiv) under nitrogen atmosphere. The resulting mixture was stirred for 20 min at room temperature. To this was added a solution of phthalimidoacetone (101.5 g, 0.5 mol) in 700 mL of THF (anhydrous) over a period of 30 min. The resulting reaction mixture was stirred for 2 h at room temperature and then heated at 55 oC for 1 h. To this was added sodium methoxide solution (25% solution, 172 mL, 1.5 equiv) at 55 oC over a period of 40 min. After 3 h HPLC/MS indicated starting material and intermediates were converted to the pyrrole. A crude sample of pyrrole was obtained by extractive aqueous workup. To the above reaction mixture was added ethyl formate (200.8 mL, 5 equiv) over a period of 20 min followed by sodium methoxide (25% solution, 324 g, 3 equiv). The resulting reaction mixture was heated for 7 h at 55 oC at which time
16 of 28
04/09/13 12:09 AM
HPLC/MS indicated that the pyrrole was converted to the pyrrolopyrimidinone. The reaction mixture was diluted with 1.5 L water, heated at 60 oC for 1 h, and then concentrated to small volume (~1.5 L). Solution assay indicated that pyrrolopyrimidinone was formed in 75% solution yield. This solution was neutralized to pH 7.5 with 6 N aq HCl, cooled to about 5 oC, and held at this temperature for 30 min. Solids were filtered, washed with water, dried at 50 oC under vaccum overnight to give the pyrrolopyrimidinone as a light brown solid (45.8 g, 61% yield, purity: 99.0% by HPLC area). quinolone synthesis
Organic Process Research & Development 2006, 10, 1157-1166 Knoevenagel condensation of aldehyde with methyl cyanoacetate, Michael addition of the pyridinium yield to the condensation product, and cyclization of the Michael adduct.
17 of 28
04/09/13 12:09 AM
5-Cyano-1,2,3,4-tetrahydro-6-hydroxy-4-(2-methylphenyl)- 2-oxo-1,3-bipyridinium, Inner Salt . A 3000 mL four-necked flask (with a 250 mL pressure equilibrating addition funnel/dry N2 adapter, septum with a Teflon-coated thermocouple, Teflon paddle stirrer/glass shaft, and stopper) is charged with 115.6 mL (120.15 g, 1.0 mol) of o-tolualdehyde, 87.9 mL (99.09 g, 1.0 mol) of methyl cyanoacetate, the suspension of 172.61 g (1.0 mol) of pyridinium salt in 300 mL of isopropanol , and 1.7 L of methanol. The addition funnel is charged with 153.3 mL (111.3 g, 1.1 mol) of triethylamine. The amine is added over 24 min at 170 rpm and 20-25 C (intermittent ice-water bath). The resulting mixture is stirred at 25-30 C for 24 h. The precipitate is suction filtered, washed with 500 mL of 25 C methanol, 500 mL of 25 C toluene, and 500 mL of 25 C hexanes, and then air-dried 18 h at 25 C to afford 284.69 g (93.2%) of product as a bright yellow solid.
Organic Process Research & Development 2006, 10, 1167-1171 (3-(2-Methoxy-4-trifluoromethyl)-N-(5,7-dimethoxy-[1,2,4]triazolo[1,5-a]pyrimidin2-yl)pyridinesulfonamide). Hydroxyguanidine (2.0 g, 4.4 mmol) was slurried in CH3CN (20 mL) and cooled to -5 C under N2.Potassium carbonate (1.38 g, 10 mmol) was added, followed by dropwise addition of phosgene in toluene (ca. 20%, 2.6mL, ca. 4.9 mmol). The reaction was stirred at this temperature for 40 min at which time HPLC analysis indicated 15% of hydroxyguanidine remained. After stirring an additional 30 min HPLC analysis indicated 15% of hydroxyguanidine still remained. Additional phosgene/toluene (0.25 mL) was added and the reaction stirred at -5 C for 30 min at which time HPLC indicated that less than 2% of hydroxyguanidine remained. The white slurry was warmed to room temperature and allowed to stir overnight. HPLC analysis indicated that 9% of oxadiazolone remained. The white slurry was warmed to 35 C and was held there for 30 min at which time HPLC indicated the cyclization was complete. The slurry was cooled to room temperature and then treated with water (15 mL). The pH was adjusted to 2 by dropwise addition of concentrated HCl. The volatiles were removed on the rotary evaporator (35 C/25 mmHg), leaving a white slurry. The solid was collected by filtration and washed with a little water to give 2.74 g of crude wet material. HPLC analysis of the wet material revealed three major peaks: (1) rt 3.07 min, 7.9%; HRMS indicated this impurity is an isomer of desired product; (2) rt 3.52 min, 79.5% desired product; (3) rt 5.84 min, 10.9%, toluene. The wet solid was dried to a constant weight (80 C/ 25 mmHg), providing the product as a white solid (1.84 g). A 1.0 g sample was dissolved in boiling CH3OH/CH3CN (60 mL/10 mL) and then concentrated to a total volume of 15 mL. The mixture was cooled in an ice bath, and the resulting crystals were collected and washed with a little CH3OH. The material was dried to a constant weight (5 mmHg/room temperature) providing desired product as white needles (944 mg, 91.1% purity via external standard HPLC analysis, 83% yield based on recrystallization of all of the 1.84 g of crude material). This sample contained 2.3 area % of an isomer of desired product and 6.6 wt % CH3CN. Leimgruber-Batcho indole synthesis for 6-benzyloxyindole
18 of 28
04/09/13 12:09 AM
Organic Process Research & Development 2006, 10, 1178-1183 Laboratory Procedure of Hydrogenation to Obtain the Indole. To a mixture of enamine (5.00 g, 15.4 mmol), iron(II) acetate (28 mg, 0.154 mmol), and 5% Rh/C (317 mg, 0.154 mmol), tetrahydrofuran (THF) (100 mL) was added. After the atmosphere was displaced with N2 followed by H2, the mixture was stirred for 15 h at rt under atmospheric pressure of H2. After the atmosphere was replaced with N2, aqueous NH3 (_14%, 20 mL) was added. The following workup operations were conducted under N2. After stirring for 20 min, the mixture was filtered to remove the catalyst, which was washed with THF (50 mL). The combined solutions were extracted with toluene (50 mL). The organic layer obtained was washed with aqueous citric acid (10%, 50 g), aqueous sodium bicarbonate (5%, 50 g), and brine (20%, 50 g). The yield of the target indole was determined by HPLC (3.30 assay g, 96% yield). The solution was concentrated in vacuo. The residue was dissolved in toluene (100 mL) and filtered through a pad of SiO2 (5 g), and the silica gel was washed with toluene (100 mL). The combined toluene solutions were analyzed by HPLC (3.22 assay g, 94% yield), and then concentrated in vacuo. The residual solids were suspended in toluene (5 mL) and heptane (13 mL) and heated to 90 C to dissolve the solids. The homogeneous solution was cooled to 63 C, followed by seeding with a crystal of indole to form a seed bed. The slurry formed was stirred for 1 h at 60-65 C, heptane (37 mL) was added over a period of 2 h, and the solution was gradually cooled to rt. After standing overnight, the slurry was filtered, washed with heptane-toluene (10:1) (5.5 mL) and heptane (5.5 mL), and dried in vacuo at 40 C overnight. The target indole was obtained as colorless crystals (2.94 g, 86% yield with 99.9 area % purity by HPLC). Knorr Cyclization Pyrrole
19 of 28
04/09/13 12:09 AM
Lawessons reagent
4(R)-[1(S)-[tert-(Butoxycarbonyl)amino]ethyl]pyrrolidinone- 2-thione. A slurry of starting material (254 g, 1.114 mol) in THF (2.285 L) in a 5-L reaction flask under nitrogen was treated with Lawessons Reagent (225 g, 0.557 mol, 0.5 equiv) in three portions over a 15-min period. The reaction was stirred at room temperature for 4 h. The solid was collected by filtration and washed with toluene (200 mL). The collected solid was dried (45 C, 35 mmHg) to give 173 g of desired product, (HPLC1) ) 3.03 min, 96.71 by area %. The filtrates were combined and held at -10 C for 2 days, solid was collected by filtration, washed with toluene (50 mL), and dried as before to give 70 g of product, 89.6% total yield, (HPLC1) ) 2.96 min, 92.35 area %.
Noyori hydrogenation
20 of 28
04/09/13 12:09 AM
Transsulfamoylation Reaction
Heck Reaction
21 of 28
04/09/13 12:09 AM
In a typical process, 3-cyanobenzaldehyde (1 equiv) and ammonium acetate (2.5 equiv) are slurried together in ethanol (18 rel vols). A solution of dione (0.9 equiv) in ethanol (6 rel vols) is added slowly, followed by allyl ester (1.1 equiv) and more ammonium acetate (2.5 equiv) in ethanol (11 rel vols). The mixture is heated to reflux for 3.5 h, and then excess ethanol is removed by distillation under vacuum until 18 rel vols remains. Water (24 rel vols) is added to precipitate the product, which is isolated by filtration and washed with water (6 rel vols) and MTBE (6 rel vols, twice).
Reductive alkylation
22 of 28
04/09/13 12:09 AM
allowed to stir at room temperature for 30 min and was then charged with concentrated aqueous HCl (0.25 gal). The reactor was charged with water (13.7 kg) and then seeded with 1 (2 g) to initiate crystallization. The mixture was stirred for 20 h and then was filtered; the cake rinsed with THF (4 gal) and then water (7 gal). The wet cake was transferred to a clean reactor and charged with water (18 gal) and stirred for 2 h. The slurry was filtered, washed with water (12 gal), and dried at 50 C for 5 days to provide 7.3 kg (56%) of desired product as a white crystalline solid (purity 90% by HPLC analysis).
Beta-ketoester synthesis
Nuceophilic substitution
23 of 28
04/09/13 12:09 AM
4-(4-Nitrobenzyl)morpholine To a 2-L, three-neck, round-bottom flask, fitted with mechanical stirring, thermocouple, 250-mL addition funnel, and nitrogen inlet were charged 4-nitrobenzylbromide (100 g, 463 mmol) (Caution. Strong irritant and lachrymator! Weigh in hood with door down and transport in stoppered flask!) and 500 mL of toluene. A solution of morpholine (121 g, 1389 mmol) in 100 mL of toluene was added dropwise, keeping the temperature less than 50 C. The solution was rinsed in with 50 mL of toluene. The resulting solution was stirred at less than 50 C until the reaction was complete. TLC analysis; add 1 mL of reaction mixture to 1 mL of water, spot upper layer and elute with 1:1 ethyl acetate/heptane, visualize with UV. When complete, 500 mL of water was added, and the phases were separated. The organic phase was washed with 500 mL of saturated sodium bicarbonate. The organic phase was concentrated to less than 150 mL volume on the rotovap with a 60-70 C bath. Isopar C (500 mL) was added to crystallize the product. The slurry was stirred at 20-25 C for 30 min, filtered, and washed with 100 mL of Isopar C. The product was dried at 45 C in a vacuum oven overnight. The yield was 96.0 g, 93%, of desired product: mp 80.3-82.1 C. HPLC purity 95%. mild conversion of a quinoline N-oxide to a 2-aminoquinoline
Transfer alkylation
24 of 28
04/09/13 12:09 AM
Curtius rearrangement
LAH reduction
25 of 28
04/09/13 12:09 AM
In a 100-gal glass reactor were warmed sodium triacetoxyborohydride (18.5 kg, 87.3 mol) and DMSO (71 kg) to 40 C until homogeneous. The batch was cooled to 12 C, and (S)-3-(4-fluorobenzyl)piperidine 2-(R)-mandelate salt (20.2 kg, 58.5 mol) was added. In a separate reactor, (1R,2R)-2-(benzyloxycarbonylamino)cyclohexanecarboxaldehyde (15.3 kg, 58.5 mol) and DMSO (33 kg) were mixed to give a homogeneous solution. The aldehyde solution was added over 2 h to the piperidine solution while keeping the temperature at 10-14 C. After the addition the reaction was stirred for an additional hour and then quenched with 6 N aqueous HCl solution (8.4 kg) at 20-24 C. To the quenched reaction mass were sequentially added iPrOAc (59 kg), water (67 kg), and 15% aqueous sodium hydroxide solution (50 kg). The mandelic acid was removed in the aqueous solution. The product was extracted into the iPrOAc, which was washed with 10% brine (40 kg) and water (45 kg). This iPrOAc solution containing (1R,2S)-1-(benzyloxycarbonyl) amino2-{[3(S)-(4 fluorobenzyl)piperidinyl]methyl}-cyclohexane was used in next reaction without purification.
Org. Process Res. Dev., 10 (1), 36 -45, 2006 8-Bromo-6-methylquinoline. Sodium m-nitrobenzenesulfonate (2.67 kg, 11.8 mol) was added to methanesulfonic acid (10 L) at 20 C followed by iron sulfate heptahydrate (156.8 g, 0.564 mol). This addition is slightly exothermic and raised the temperature to 27 C. To the resulting mixture was added 2-bromo4-methylaniline (3.50 kg, 18.8 mol) through an addition funnel with stirring over 30 min, flushed with methanesulfonic acid (0.5 L), and agitated until the solid dissolved (~15 min). The addition is exothermic, and the temperature reached ~60 C after the addition. The reaction mixture was heated to 118-125 C, and glycerol (4.33 kg, 47.0 mol) was added through an addition funnel over 4-8 h. After stirring at 125-133 C for 10-16 h, the mixture was cooled to ~80 C and diluted with cold DI water (10 L) keeping the temperature <90 C. After cooling to ~20 C with an ice bath, the mixture was neutralized with cold aqueous NaOH (10 M, 15.6 L). Solka floc (500 g) was added, and the mixture was further neutralized with 1 M aqueous NaHCO3 (1 M, 16 L). The mixture was cooled to 10-20 C, and MTBE (30 L) was added. After stirring for 4-6 h, the mixture was filtered through a centrifuge (10- m bag), and the phases were separated. The (top) MTBE layer was washed with 20 L of brine (95% saturation). The aqueous layers were back extracted with MTBE, and the organic phases were combined (3.97 kg of product as a 7-9 wt % MTBE solution in 95% yield). For characterization purposes, the hydrochloride salt of 8-bromo-6-methylquinoline can be prepared. 1,2,4-Oxadiazole Synthesis
26 of 28
04/09/13 12:09 AM
Org. Process Res. Dev., 10 (1), 36 -45, 2006 3-Methyl-5-[4-(methylsulfonyl)benzyl]-1,2,4-oxadiazole. Hydroxybenzotriazole hydrate (2.06 kg, 13.4 mol) was suspended in acetonitrile (25 L), and 2.5 L was distilled off at 1 atm under nitrogen to azeotropically remove water. [CAUTION: Hydroxybenzotriazole will decompose, possibly violently, if heated above its melting point (155-160 C).] After cooling to 25-30 C, 4-methylsulfonylphenylacetic acid (2.5 kg, 11.67 mol) was added, followed by EDC hydrochloride (2.68 kg, 14.0 mol). The resulting mixture was stirred at 20-30 C for 30 min. Methylamidoxime (1.14 kg, 14.0 mol) was added to the slurry over 10 min. The resulting mixture was then heated at reflux for 12 h. The solution was solvent switched to ethyl acetate under reduced pressure (100-200 mBar, 40-50 C) by continuous distillation of ~40 L of ethyl acetate and concentrated to a final volume of ~27 L. After cooling to ~20 C, aqueous NaHCO3 (1 M, 20 L) was added slowly with vigorous stirring. The aqueous layer was removed, and the organic layer was washed with DI water (7.5 L). The aqueous solutions were back extracted with 17.5 L of ethyl acetate. The combined ethyl acetate solution was concentrated to ~7 L (100-200 mBar, 50-70 C) and diluted with 2-propanol (17.5 L). The solution was further concentrated to ~12.5 L. The solution was allowed to cool to 10-20 C, and the desired product precipitated. After stirring for 2 h the mixture was filtered; the solid was washed with 2 2.5 L of 2-propanol and then air-dried for 2 h. The solid was further dried in an oven at 30-35 C under vacuum (N2 sweep) to constant weight. Isolated yield: 2.6 kg, 88%. Friedel-Crafts acetylations
Org. Process Res. Dev., 10 (1), 135 -141, 2006 N-(5-Acetyl-2,3-dihydro-1H-inden-2-yl)-2,2,2-trifluoroacetamide. Regioselective Friedel-Crafts Acetylation of starting material in Dichloromethane. A 1-L, four-necked, round-bottomed flask (rinsed with dichloromethane), equipped with a mechanical stirrer, digital thermometer, cooling bath, and nitrogen inlet-outlet, was charged with aluminum chloride (102.0 g, 765.0 mmol) and dichloromethane (280.0 mL) at 20-25 C. The slurry was cooled to an internal temperature at 0-5 C, and acetyl chloride (72.0 g, 917.0 mmol) was added over 20 min while maintaining the temperature at 0-5 C. The white suspension was stirred at 0-5 C for 15 min and N-(2,3dihydro-1H-inden-2-yl)-2,2,2-trifluoroacetamide (70.0 g, 305.0 mmol) was added in five equal portions (14.0 g each) at 5-min intervals (total time = 25 min) while maintaining the internal temperature at 0-7 C. Dichloromethane (20.0 mL) was added to wash off any solids sticking to the wall of the flask. The resulting tan solution was stirred at 0-5 C for 1 h and then added to 1 N HCl (500.0 mL, precooled to 0-5 C) in a 2-L, threenecked, round-bottomed flask, equipped with a mechanical stirrer, digital thermometer, and a cooling bath, while maintaining the temperature at 0-25 C. The 1-L, round-bottomed flask and the transferring tube were rinsed with dichloromethane (40.0 mL) and the dichloromethane added to the reaction mixture. The biphasic reaction mixture was warmed to 20-25 C. The layers were separated, and the organic layer was washed with water (100.0 mL). The organic layer was transferred to a 2-L, four-necked, round-bottomed flask, equipped with a mechanical stirrer, digital thermometer, addition funnel, nitrogen inlet-outlet, a reflux condenser, and a heating mantle. The solution was warmed to an internal temperature at 40 3 C to achieve gentle refluxing. Heptane (1.2 L) was added
27 of 28
04/09/13 12:09 AM
slowly over a period of 50 min while maintaining the temperature at 40-55 C to maintain a gentle reflux. The resulting slurry was stirred at 55-58 C for an additional 30 min. The suspension was cooled to 20-25 C over a period of 30 min with efficient stirring and stirred at 20-25 C for an additional 2 h. The solids were collected by filtration, washed with a mixture of dichloromethane/heptane (2 100.0 mL, 20:80 v/v), and dried at 60-65 C in vacuo to afford N-(5-acetyl-2,3-dihydro-1H-inden-2-yl)-2,2,2-trifluoroacetamide (73.4 g, 88.7%)
28 of 28
04/09/13 12:09 AM