AEC-2 SOP Template

ANIMAL ETHICS COMMITTEE APPROVED STANDARD OPERATING PROCEDURE (SOP) FOR USE OF ANIMALS Where appropriately applied, Standard Operating Procedures (SOP) may facilitate the preparation of proposals by investigators and teachers. There is a risk that the use of SOP may reduce the rigour ith hich procedures are considered by the !"# and therefore, SOP should only be referred to in proposals under the follo ing circumstances. i) ii) iii) iv) v) ne SOP must be approved by the !"# before implementation$ SOP must include in the title the date on hich there ere last approved or revie ed and be revie ed regularly by the !"# at least every three years$ !"# members must have ready access to copies of all current SOP$ %nvestigators or teachers named on a proposal must have the necessary skills to implement a SOP$ and &ariations to a SOP must be detailed in the proposal

'efer to (.(.)* of the #ode) for further information. Provide details i ! "#olo!red $a els ONL% Revie* d!e) +!l, -./0 C!rre t versio A!t1or) Sandra Simpson Na'e o2 $ro#ed!re) ,ear) -./-

Sta dard O$erati & Pro#ed!re N!'(er)

Pre'edi#atio

a d i 3e#ta(le a aest1esia i

&!i ea $i& a d rat

Ai' o2 $ro#ed!re) +or learners to gain e,perience in pre-operative assessment of animals, administration of pre-medication and in.ectable anaesthetic agents and monitoring of general anaesthesia in the rat or guinea pig species. Releva t $erso al $rote#tive e4!i$'e t re4!ired) /aboratory coat 0loves #losed shoes Asso#iated SOPs (list !'(er a d title))

Australian Code of Practice for the care and use of animals for scientific purposes

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AEC-2 SOP Template

Sta dard O$erati & Pro#ed!re N!'(er) Revie* d!e) +!l, -./0 C!rre t versio SOP SOP SOP SOP SOP SOP SOP SOP (1 2andling, restraining and transporting rats 31 2andling, restraining and transporting guinea pigs 331Weighing animals to monitor health 451Prepare animals for premedication and anaesthesia 51 %ntraperitoneal in.ection 61 Subcutaneous in.ection 551%ntraperitoneal in.ection in rat and guinea pig using to el 441!naesthetic complications (if re7uired) ,ear) -./-

rap restraint techni7ue

Prere4!isites)

Students to have undergone training in animal handling and restraint techni7ues appropriate to the species in.ection techni7ues1 S# 8 %P E4!i$'e t a d 'aterials)

!nimals1 9se only healthy animals. !ll animals undergo health assessment prior to practical. #linical health assessment records completed 'ats to be min eight of ((:gms 0uinea pigs1 adult ONL% SPRAGUE DA5LE% RATS TO 6E USED7 UNDER NO CIRCUMSTANCE ARE LONG EVANS RATS TO 6E USED IN ANAESTHETIC PRACS8

"7uipment1 )ml syringes needles (;-(5 gauge );mm, sharps disposal container, stethoscopes, rectal thermometers, eight scales, heat pads, recovery bo, <aterials1 /acrilube for eye lubrication, vaseline or => .elly to lubricate thermometer, Sterile :.?@ saline !naesthetic agents1 =etamine hydrochloride )::mgAml, BylaCine (: mgAml, !tipamisole 3mgAml !naesthetic record, pen Details o2 t1e $ro#ed!re) include the environment where the task will be undertaken.

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AEC-2 SOP Template

Sta dard O$erati & Pro#ed!re N!'(er) Revie* d!e) +!l, -./0 C!rre t versio Lo#atio ) "6.;:6 N!'(er o2 a i'als $er sessio ) ma,imum of ; rats or guinea pigs. Students groups of ( or ; to complete the practical ork in ,ear) -./-

Ti'e) !naesthetic practical classes should be scheduled for morning sessions to ensure sufficient time to assess that animals have recovered from anaesthesia before being left overnight STAGE /) PRE"ANAESTHETIC PREPARATION Dotify !nimal 2ouse staff to ithhold food and ater for )-( hours prior to anaesthesia. ). Select and prepare the site for anaesthesia and turn on heat pad (. Prepare recovery bo,1 prepare a clean bo, ith suitable bedding and place on a heat pad. Eedding needs to be absorbent, not able to occlude nose or eyes or able to cause suffocation of animals during recovery 98 Pre"a aest1esia e:a'i atio ) Perform a pre-anaesthesia health check of the animal. The follo ing data is recorded on the anaesthetic record1 Weight #olour of mucous membranes #apillary refill time Observation of respiratory rate and pattern <ental alertness

!ny abnormalities detected are to be recorded and also reported to the trainerAsupervisor. ;8 Cal#!late t1e dose o2 $re'ed to (e &ive ) students are to calculate the re7uired volume of atropine. Pre'edi#a t !tropine G!i ea Pi& :.:3 mgAkg S# Rat :.:3 mgAkg S#

'eference1 +lecknell, P.! F/aboratory !nimal !naesthesiaG, (nd "dition 08 C1e#< dose #al#!latio *it1 s!$ervisor

5. Ad'i ister $re'edi#atio ) !dminister premed by subcutaneous (S#) in.ection using )ml syringe and (;-(5g, );mm needle (SOP 6). 'ecord time of administration and dose (ml) of premed on anaesthetic record.

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AEC-2 SOP Template

Sta dard O$erati & Pro#ed!re N!'(er) Revie* d!e) +!l, -./0 C!rre t versio ,ear) -./-

STAGE -) ANAESTHESIA ). Cal#!late dose o2 a aest1eti# a&e t to (e &ive ) students are to calculate re7uired volumes of ketamine and ,ylaCine to be in.ected. !naesthetic !gent =etamine hydrochloride S= BylaCine G!i ea Pi& 4: mgAkg %P 3 mgAkg %P Rat *3 -):: mgAkg %P ): mgAkg %P

'eference1 +lecknell, P.! F/aboratory !nimal !naesthesiaG, (nd "dition =etamine is classified as an S6 drug. Storage and dispensing of this drug must be in accordance of the legislative re7uirements of this class of drugs. (. Cal#!late dose o2 ati$a'isole1 for reversing the affects of ,ylaCine1 dose rate H ) mgAkg 98 C1e#< dose #al#!latio s *it1 s!$ervisor) Prepare a )ml syringe containing ketamineA ,ylaCine and a %ml syringe containing atipamisole. Syringes must be labelled ith contents. !tipamisole should be prepared in advance in the event of an anaesthetic complication re7uiring rapid lightening of anaesthesia.

4. Ad'i ister a aest1esia) !dminister anaesthetic agents by intraperitoneal (%P) in.ection )3 mins after premed using )ml syringe and (;-(5g, ); mm needle (SOP 5 or 55). /oss of righting refle, should occur ithin ;-3 minutes follo ing correct placement of %P in.ection. Students are to commence monitoring anaesthesia and recording of information on the anaesthetic record after righting refle, is lost. Students are to monitor and record, respiratory rate, body temperature, capillary refill time, colour of mucous membranes and depth of anaesthesia on anaesthetic record at least every 3 minutes. Iepth of anaesthesia is determined by the level of muscle rela,ation, presence or absence of the pedal ithdra al refle, presence or absence of response to tail pinch in the rat presence or absence of the palpebral refle, location of the iris

3. Ad'i ister La#ril!(e to (ot1 e,es) The cornea is protected by the administration of /acrilube to both eyes hen animal is anaesthetised. 5. Preve tio o2 1,$ot1er'ia d!ri & a aest1esia) animals are to be placed on a heat pad as soon as anaesthesia commences. 'ecovery bo, is placed on a heat pad before anaesthesia commences. Students are e,pected to demonstrate and record methods of minimising loss of body temperature during anaesthesia on the anaesthetic record. Eody temperature is monitored during anaesthesia and recorded on the anaesthetic record.

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AEC-2 SOP Template

Sta dard O$erati & Pro#ed!re N!'(er) Revie* d!e) +!l, -./0

C!rre t versio ,ear) -./6rie2 o!tli e o2 'o itori & $ro#ed!res) use step by step instructions a d i #l!de t1e $re7 d!ri & a d $ost $ro#ed!re i 2or'atio ). Pre-anaesthetic monitoring1 completion of pre anaesthetic e,amination. !nimals are observed at a distance to assess behaviour and alertness. !nimalJs bo, is also inspected for signs of diarrhoea or abnormal discharges. !nimals are then removed from their housing bo, and inspected individually for sleek smooth coat, absence of discharges from e,ternal orifices and normal pattern of respiration. %nformation is recorded on !nimal 2ealth Sheet and anaesthetic record. !ny animals sho ing clinical signs of disease ill be ithdra n from the practical. (. D!ri & anaesthesia1 students monitor the depth of anaesthesia and the vital signs at least every 3 mins. %nformation is recorded information on anaesthetic record. ;. Post) -anaesthetic monitoring1 students to continue to record depth of anaesthesia and vital signs until the righting refle, returns and the animal resumes mobility. !nimal facility staff to monitor food and ater intake over the ne,t (4-46 hours after return to animal facility

Clea

!$ a d *aste dis$osal &!ideli es)

Waste disposal1 all needles and syringes are disposed of in yello , rigid alled clinical aste sharps container #lean up1 bench tops and heat pads are iped ith +):. 0loves are disposed of in general aste container A$$roval 2or Pro#ed!re)

Signed1 Iate1 Revie* D!e)

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