DOGMA CENTRAL DE LA BIOLOGA MOLECULAR

PROCARIOTAS

EUCARIOTAS

REPLICACIN DEL ADN GENMICO

Etapas de la Replicacin
(Enzimas que intervienen)
1.

2.

Desenrrollamiento y apertura de la doble hlice. Sntesis de dos nuevas cadenas de ADN.

3.
4.

Correccin de errores. Terminacin

ORIGEN DE REPLICACIN

ORIGEN DE REPLICACIN PROCARIOTES

DNA replication is bi-directional

of replication

[3H] thymidine

[3H] thymidine

Replication of Drosophila DNA

Label first with high radioactive nucleotides, which is followed by weak radioactive nucleotides

DNA replication in Newt

REPLICACIN DEL DNA EN EUCARIOTES

Reaccin catalizada por la ADN polimerasa

Reaccin catalizada por la ADN polimerasa

Reaccin catalizada por la ADN polimerasa

Cebador

Desosinucleotidos

Hidroxilo libre del extrem 3`

Hebra molde

Horquillas de Replicacin

Diagrama de una horquilla de replicacin durante la sntesis de ADN

3
Cadena directora

Cadena seguidora

HORQUILLA DE REPLICACIN

DNA polymerase III is important for DNA replication in bacteria Mutants with a defect in the polA gene (encoding polymerase I) are viable, suggesting that pol I is not a major DNA replicating enzyme Mutants with a defect in the DnaE gene (encoding polymerase III) are inviable, suggesting that pol III is the DNA replicating enzyme in bacteria. Pol II is also dispensable for DNA replication.

Video

Fidelidad de replicacin

Correccin de errores

Endonucleasa

La replicacin un proceso complejo

Fase C

Fase D

EUCARIOTAS

REPLICACIN DEL DNA EN EUCARIOTES

Replicacin del ADN cicular y lineal

Replicones Simples o Mltiples

Replication Facts
DNA

has to be copied before a cell divides DNA is copied during the S or synthesis phase of interphase New cells will need identical DNA strands
copyright cmassengale 58

Synthesis Phase (S phase)

S

phase during interphase of the cell cycle Nucleus of eukaryotes

DNA replication takes place in the S phase. phase

G1

interphase

G2

Mitosis

-prophase -metaphase -anaphase -telophase

copyright cmassengale 59

Sntesis Hebra conductora o lder Sntesis Hebra rezagada

Sntesis Hebra conductora

Sntesis Hebra rezagada

REPLICACIN DE LA CADENA CONTINUA

Replicacin en telmeros

Telmeros

Unidirectional Replication (colE1)

Replicacin en virus

Efectos del virus sobre la clula

Replicacin de los virus de ADN

Horquilla

Molcula RF doble

Cebador de ARN

Rolling circle replication

Single-stranded DNA phage: fX174

l Phage replication

REPLICACIN ds DNA

Replicacin en virus de ARN

REPLICACIN ss RNA-

Stages of replication - RNA virus Alternative A: (-) RNA virus

3' 5' (-) stranded RNA

(+) stranded RNA = mRNA

Viral RNA replicase

Protein 5' mRNA (+) stranded RNA 3'

Alternative B: (+) RNA virus (Retrovirus)

5' 3' (+) stranded RNA

Alternative C: Retrovirus; (+) RNA virus

5' 3' (+) stranded RNA

Viral RNA replicase

3' 5' (-) stranded RNA
3' 5' 5' 3' (-) DNA strand

Viral RNA replicase

Retroviral Reverse transcriptase

3' 5'

5' 5' 5' (+) stranded RNA incl. mRNA

3' 3' 3' Protein

3' 5'

5' 3'

Further replication as a DNA virus

El mapa gentico de un retrovirus :

VIH

IMPORTANCIA DE CONOCER LOS PASOS DE LA REPLICACION VIRAL

Crecimiento, purificacion y caracterizacion de los virus de importancia medica. Conocer las etapas de la enfermedad para la toma de muestra para el DIAGNOSTICO

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