Escolar Documentos
Profissional Documentos
Cultura Documentos
References
2. Cleland, Meths Enzymol. 87, 390-405 (1982). pH analysis. The remainder of this
volume has considerable useful information for the practicing kineticist.
3. Cleland, adv. Enzymol. 45, 273-387 (1977). Most of what the normal kineticist will
ever have to know is to be found here.
5. King, E.L & Altman C. J. Phys. Chem. 60, 1375-1378 (1956). The King-Altman
method for driving kinetic equations.
6. Cleland, Biochemistry 14, 3220 (1975). The net rate constant method for the analysis
of linear mechanisms.
7. Cha, J. Biol. Chem. 243, 820-825 (1968). Rapid equilibrium simplification of King
Altman procedure.
9. Northrop, D.B. j. Chem. Ed. 75, 1153-1157 (1998). On the meaning of KM and V/K.
Derivatisation of rapid equilibrium equations
k 2 < < k -1
v= f ([S])
Application
Life can be more complex than that
Initial Velocity Measurements
3D Matrix Fitting
v = Vmax[A][B]/(KiaKB+KA[B]+KB[A]+[A][B])
60
50
Exp. v
40
2.0 mM BnOH
4.5
V (s-1)
9.5
30
14
19
20
10
0 1 2 3 4 5
+
[NAD ] (mM)
0.3
]/V (mM*min.)
0.2
[NAD +
0.1
0
-1 0 1 2 3 4 5
+
[NAD ] (mM)
Transient kinetics: flow apparatuses
Stoped flow
Quenched flow
Pulse flow
Proton transfer rate constants (25˚C, s -1 M-1)