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42, Issue 1, pp 47-54

Ultrastructural immunolocalization of a cartilage-specific proteoglycan, aggrecan, in salivary pleomorphic adenomas

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Abstract
A pleomorphic adenoma (PA) is the most common epithelial tumor in the salivary glands, but it frequently shows a mesenchyme-like histology, including the presence of myxoid and chondroid areas. Cartilage-specific matrix proteins are deposited in PA. Aggrecan is a major component of cartilage-specific proteoglycans. The present study examined the ultrastructure of the stromal areas in ten salivary PA specimens and investigated the distribution of aggrecan by immunoelectron microscopy. Aggrecan was deposited in the myxoid and chondroid stroma of PA. Ultrastructural observations revealed many proteoglycan cores and fibrils in the myxoid stroma and some spindle-shaped neoplastic myoepithelial cells with vacuoles and actin filaments in the myxoid areas. By immunoelectron microscopy, positivity for aggrecan was observed in the vacuoles of neoplastic myoepithelial cells, which coexisted with the viscous materials, and it was also frequently seen in electron-dense crystals in the myxoid stroma. These findings suggest that neoplastic myoepithelial cells produce aggrecan and release it from vacuoles, and aggrecan is then deposited in the myxoid stroma. Aggrecan deposition is therefore considered to play an important role in the formation of the mesenchyme-like stroma, especially the myxoid stroma. Page %P Page 1

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About this Article

Title Ultrastructural immunolocalization of a cartilage-specific proteoglycan, aggrecan, in salivary pleomorphic adenomas Journal Medical Molecular Morphology Volume 42, Issue 1 , pp 47-54 Cover Date 2009-03-01 DOI 10.1007/s00795-008-0429-4 Print ISSN 1860-1480 Online ISSN 1860-1499 Publisher Springer Japan Additional Links Register for Journal Updates Editorial Board About This Journal Manuscript Submission Topics Molecular Medicine Biological Microscopy

Pathology Medical Microbiology Keywords Aggrecan Pheomorphic adenoma Immunoelectron microscopy Ultrastructure Salivary gland Authors Koji Muramatsu (1) (2) Kimihide Kusafuka (2) Hideto Watanabe (3) Toru Mochizuki (1) Takashi Nakajima (2) Author Affiliations 1. Cancer Diagnostics Research Division, Shizuoka Cancer Center Hospital and Research Institute, Shizuoka, Japan 2. Pathology Division, Shizuoka Cancer Center Hospital and Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka, 411-8777, Japan 3. Institute for Molecular Sciences of Medicine, Aichi Medical University, Aichi, Japan

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