Code No: RR412302 Set No.

1
IV B.Tech I Semester Supplementary Examinations, February 2007
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What are the various products of r DNA and their economic assessment of various
proteins? [16]

2. Describe about primary factors affecting separation? [16]

3. Write in detail about physico-mechnaical methods of Cell disruption? [16]

4. Explain about Cell aggregation and Flocculation? [16]

5. Write about different modes of operation of filters? [16]

6. Explain the various factors that influence protein solubility? [16]

7. Write short notes on:

(a) Paper chromatography

(b) SDS-PAGE [8+8]

8. Explain about the process of Dialysis? [16]

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Code No: RR412302 Set No. 2
IV B.Tech I Semester Supplementary Examinations, February 2007
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe about preliminary economic analysis or cost analysis performed in Bio-

process? [16]

2. Explain the role of Downstream processing and different market sectors in Biotech-
nology? [16]

3. Explain with a neat sketch the working of Homogenizer? [16]

4. Explain about Cell aggregation and Flocculation? [16]

5. Write short notes on:

(a) Reverse Osmosis

(b) Ultra filtration [8+8]

6. Describe the importance of process integration and whole broth treatment and
in-situ recovery strategies for product recovery? [16]

7. Write short notes on:

(a) Paper chromatography

(b) SDS-PAGE [8+8]

8. Explain about the process of Dialysis? [16]

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Code No: RR412302 Set No. 3
IV B.Tech I Semester Supplementary Examinations, February 2007
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Examine the continuing role which economics plays in Bioprocess research,development

and commercialization? [16]

2. Explain the role of Downstream processing in Biotechnology? [16]

3. Write in detail about physico-mechnaical methods of Cell disruption? [16]

4. What are the various techniques employed in recovery of Bioproducts? Explain in

brief about them? [16]

5. Write about different modes of operation of filters? [16]

6. Discuss about Colloidal stability of protein solutions? [16]

7. Write short notes on:

(a) Rocket Immuno electrophoresis

(b) Capillary electrophoresis [8+8]

8. Write short notes on:

(a) Secondary nucleation

(b) Heterogeneous nucleation [8+8]

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Code No: RR412302 Set No. 4
IV B.Tech I Semester Supplementary Examinations, February 2007
DOWN STREAM PROCESSING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Examine the continuing role which economics plays in Bioprocess research,development

and commercialization? [16]

2. Explain about the Filtration technique employed for several Bioproducts? [16]

3. Write short notes on:

(a) Homogenizer
(b) Bead mill [8+8]

4. What are the Factors influencing Sedimentation rates of particles in Batch gravity?
[16]

5. How can RO and Ultra filtration complement each other? [16]

6. Describe about different techniques employed in Precipitation? [16]

7. Write short notes on:

(a) Paper chromatography

(b) SDS-PAGE [8+8]

8. Explain about the Principle and application of Gel Permeation chromatography?

[16]

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