SYLLABUS FOR M.Phil. / Pre- Ph.D.

Programmes

PAPER-1: RECENT ADVANCES IN BIOCHEMISTRY

(Effective from 2007-08)
Common for all programmes

1. Protein sequencing analysis. Chemical synthesis & modification of proteins.

2. Isolation and characterization of enzymes. Enzyme regulation – Allosteric and Covalent

modifications. Immobilized enzymes.

3. Hormone receptors and hormonal action, Second messenger systems, Role of G-Proteins in
bio-signaling, MAP Kinase pathway.

4. Stem cells –Types, culture and applications.

5. Gene therapy and Gene silencing.

6. Genetically Engineered of microorganisms

7. Nanobiotechnology and its applications.

8. Biochemistry of cancer.

9. Pathogenesis of Malaria, Filaria and HIV

10. Functional disorders of brain.

References:

1. Molecular Cell biology, 6th Ed. by Lodish, Freeman & Co

2. Agricultural Biotechnology Etd by Aric Altman, MARCEL DEKKER. Inc (Pub)
1998.
2. Plant Biotechnology by Slater and Scott. Oxford University Press.
3. Animal cell culture by R.I.Freshney (IRL Press)
4. Industrial Microbiology by Presscot abd Dunn.
5. Molecular Biology and Biotechnology. A comprehensive Desk reference; by
R.A.Meyers. (VCH Publishers)
6. Recombinant DNA technology by Watson – 2nd Edition.
 SYLLABUS FOR M.Phil. / Pre- Ph.D. Programmes

PAPER-II: RESEARCH METHODOLOGY IN BIOCHEMISTRY

(Effective from 2007-08)
Common for all programmes

1. Methods of studying intermediary metabolism and use of laboratory animals for metabolic
studies.

2. Principles and applications of Radio-isotopic techniques, ELISA, RIA, FACS

3. Principles and applications of Spectrophotometry, ESR, NMR and Mass Spectrometry.

4. Electrospray Ionization – MS, GC-MS, LC-MS, MALDI and MALDI-TOF

5. Principles and applications of Fluorescent, Scanning and Transmission Electron

Microscopy.

6. Principles and applications of preparative and Analytical ultracentrifugation.

7. Principles and applications of Ion Exchange, GLC, Gel filtration, affinity and High
pressure liquid chromatography.

8. Principles and applications of High voltage, Gel and immunoelectrophoresis, Isoelectric

focusing – 2D-Electrophoresis & PFGE, Blotting techniques.

9. Principles of experimental design, collection assembly, analysis and interpretation of

experimental data. Data presentation – tabular, graphical and diagrammatic representation.

10. Statistical applications in research – Standard deviation, standard error, analysis of

variance, linear regression, analysis, chi-square test, student ‘t’ test.

References:
1. Introductory practical Biochemistry by S.K.Sawhneyand Randhir / Singh.
2. Analytical Biochemistry by David J.Holme (Long man).
3. A Biologists guide to Principles and techniques of practical Biochemistry. Ed. By
B.D.Williams (Edward Arnold).
4. Physical Biochemistry by Frefielder (Freeman & Co)
5. Biophysical chemistry principles and techniques by Upadyay, Upadyay and Nath
(Himalaya Publishing)
6. Instrumental methods of chemical analysis by Chatwal & Andand.
7. Biostatistitcs – Daniel. (Wiley).
 SYLLABUS FOR M.Phil. / Pre- Ph.D. Programmes

PAPER III: MICROBIAL BIOCHEMISTRY

(Effective from 2007-08)

(Research Specialization paper)

1. Introduction, Historical Landmarks & Scope of Microbiology.

2. Methods of Sterilization – Heating, Radiation, chemicals and filtration.

3. Isolation and preservation of Microorganisms. Isolation procedures, pure culturing and

preservation, Maintenance and Identification procedure (Phenotypic and Phylogenetic)

4. Production of Microbial enzymes, extraction, purification and activity estimation.

5. Inoculum development, culture media, cultural condition optimization, strain improvement

via mutant selection, recombination and recombinant DNA Techniques.

6. Screening of microorganisms for new products and identification of products and

metabolites.

7. Biochemical activities of Microorganisms – extracellular enzymes, antimicrobial and

antioxidizing agents.

8. Fermentation techniques – batch fermentation, continuous fermentation, bioreactor design,

parts and their function, fermentation process and product recovery (down stream
processing).

9. Immobilization of enzymes, whole cell immobilization and methods of immobilization.

10. Application of Microbial enzymes - Industry, Pharmaceutical field, environment, food

and beverages.
 SYLLABUS FOR M.Phil. / Pre- Ph.D. Programmes

PAPER III PLANT BIOCHEMISTRY

(Effective from 2007-08)

(Research Specialization paper)

1. Plant Metabolism: Mechanism of CO2 fixation in C3 and C4 plants. N2 fixation by

symbiotic and asymbiotic method. Role of plant hormones in growth and
development of plants.

2. Plant secondary metabolites – Types, Nature, Distribution and functions.

3. Screening and isolation techniques of plant products exhibiting antioxidant and anti
microbial properties.

4. Methods for assaying antioxidant potential of enzymatic and non-enzymatic plant

products – Free radical scavenging activity, lipid peroxidase assay, DNA damage test.

5. Plant tissue culture – Propagation and production of pathogen and disease free plants.
In vitro conservation of plant genetic resources.

6. In vitro production secondary metabolites – cell cultures, Organ cultures, methods for
enhancement of secondary metabolites.

7. DNA Markers –RFLP, AFLP, RAPD, Minisatellites and Microsatellites.

8. Transgenic plants

9. Gene transfer techniques – chemical, physical and biological

10. Confirmation and analysis of transgenic plants – PCR, Southern, Northern and
Western blotting.

Reference:

1. Agricultural Biotechnology, Etd by Aric Altman; Marcel Dekker.Inc (Pub) 1998.

2. Practical applications of plat molecular Biology, by R.J.Henrey, Chapman & Hall.
3. Plant Biotechnology by Adrian Slater, Nigel scoh, Mark Fowler, Oxford Univeristy
press 2004.
4. Biotechnology secondary metabolites Etd by K.G.Ramawat and J.M.Merillon, Oxford
& IBH Publishing co., pvt ltd. 2003.
5. Genome mapping in plants by A.H.Peterson Academic press 1996.
6. Plant tissue culture theory and practice by Bhojwani S.S. and M.K.Razdan, Elsevier
publications.
7. Plant genetic engineering Etd by Don GRIERSON, Blackie & Sons ltd, 1991.
8. Plant Physiology by Lincoln Taiz and Eduardo Zeiger; Sinauer associates Inc.
publications.
 SYLLABUS FOR M.Phil. / Pre- Ph.D. Programmes

PAPER III IMMUNOLOGY

(Effective from 2007-08)
(Research Specialization paper)

1. Humoral and cell-mediated responses. Antigens, Adjuvants. Cells and organs of

immune system. Congenic and inbred strains of mice.

2. Antigen processing and presentation. Immunoglobulins. Cytokines, Interleukins,

Interferons. Complement system, MHC.

3. Antigen-Antibody interactions: techniques based on interactions between soluble

antigen and antibody - Immunodiffusion, Radial Immunodiffusion, double
immunodiffusion, Immunoelectrophoresis, Rocket Immunoelectrophoresis

4. Techniques based on interactions between particulate antigen and antibody -

Hemagglutination and Complement fixation. Other techniques - Immunoflourescence,
FACS, RIA, ELISA, Immunoblotting.

5. Hypersensitivity: immediate (type I, type II, type III) and delayed hypersensitivity
reactions.

6. Autoimmunity - organ-specific (Hashimoto’s thyroiditis) and systemic diseases

(Rheumatoid arthritis).

7. Transplantation Immunology – Immunosuppressive agents

8. Hybridoma technology: Monoclonal antibodies and their applications.

9. Vaccines-Vaccine production, conventional and new generation vaccine technologies,

DNA vaccines, recombinant vaccines, Edible vaccines.

10. Development of diagnostic kits and vaccines for AIDS and Malaria.