Rajalakshmi Engineering College Department of Biotechnology Faculty Name : Mr.M.Sankar !

ecturer" Staff co#e: B$%% Semester :&'' SEC ()B Su* co#e: B$+,-. 'mmunotechnology
/uestion Bank 0(R$ 1 ( !ist the primary an# secon#ary lymphoi# organs an# their major functions. +. Compare an# contrast the follo2ing cytoto3icity me#iate# *y $c an# N4 cells. %. Descri*e ho2 a particular B cell clone is selecte# an# acti5ate# *y (g to pro#uce (*. -. For each of the follo2ing situations in#icate 2hich type s" of lymphocytes 2oul# *e e3pecte# to proliferate rapi#ly in lymphno#es. a" Normal mouse immuni6e# 2ith a solu*le antigen. *" Neinatally themectomi6e# mouse immuni6e# 2ith protein antigen. 7. 8utline the principle of heamagglutination an# its application. .. 8utline the principle of hemolytic pla9ue forming assay an# its application. :. ;o2 #o you isolate lymphocytes an# i#entify $ cells an# B cells< =. ;o2 #o you enrich the hematopoietic stem cells using F(CS< >. ?hat is carrier effect< State its significance in 5accination< ?hat are i#iotypic anti*o#ies< 8utline their role in immune regulation. !ist out #ifferent types of immune cells an# their role in immune response Super antigen Define 2ith e3amples 0rinciple of *loo# grouping Su*cellular 5accines Catalytic anti*o#ies

0art @B A. Descri*e classical an# alternati5e path2ays of complement acti5ation an# its *iological conse9uences. ?rite a note on complement fi3ations set. +. ?ith a neat #iagram outline the intracellular e5ents occurring in $ cell 2hen (g *in#s to $CR 2hich lea#s to cytokine pro#uction. Mention any four cytokines an# their therapeutic applications. %. 8utline the approaches use# for pro#uction of humani6e# monoclonal anti*o#ies. Mention any t2o #iagnostic an# therapeutic applications monoclonal anti*o#ies. -. E3plain competiti5e an# san#2ich E!'S( an# their applications in #iagnosis. 8utline ho2 E!'S( is u#e# in (g #etection assay< 7. Descri*e the t2o tests commonly use# to assess the compati*ility of tissue graft. ?rite a not on ;!( typing. .. Descri*e the *ioassay proce#ures for follo2ing cytokines '! 1 + 'FN 1y $NF :. ?rite a note on the follo2ing emphasi6ing their 2orking principle an# (#5antages< Recom*inant 5ector 5accines. 0olysacchari#e 5accines Su*unit 5accines =. 8utline the concept of re5erse 5accinology 2ith suita*le e3ample. >.?hat are catalytic anti*o#ies< 8utline the steps in5ol5e# in pro#uction of catalytic anti*o#ies. A,. 8utline the concept of anti*o#y engineering. E3plain any t2o metho#s

Commonly use# for functional screening of com*inatorial li*rary of pepti#es. AA. /uantification of circulatory immune comple3es A+. 0rinciple an# application of cytokine *ioassays A%. $cell cloning in 5accine #e5elopment A-.Bioassay proce#ure for '!@+ an# 'FN gamma A7.8utline the process of ?estern *lot an# its uses