Escolar Documentos
Profissional Documentos
Cultura Documentos
1998
Copyright0 1998ElsevierScienceInc.
Printedin theUSA. All rightsI-ed
0271-5317/98 $19.00+ .Oo
ELSEVIER PIISO271-5317(98)00069-4
Preparation of Lectin
mal feedinaexneriments
groups of six animals each and were given treatments for 7 days
bY intragastric intubation route in two shifts of doses as
follows:
Statistical Analvsis
RESULTS
B ffec on
The normal range of jejunal DNA and RNA was 5.2850.5 and 1.54kO.2
mg/gm tissue respectively. and heat treated garlic
extracts did not effect nucleic
ASAW! ds content in general (Table
1) * However, native crude garlic extracts increased DNA to
7.53k1.4 and RNA to 2.14+0.4 mg/gm tissue (~~0.05). In contrast,
the protein contents in Sejunum was always higher irrespective of
the treatments. Maximum increase upto 40-60% in protein content
was obtained with native and heat treated garlic extracts
(PCO.01).
Table 1.
mean+S.D. (n-61,
Significantly different from controls +p<O.O5, ??
*p<O.Ol.
Table 2.
Sucrase
Units/gm tissue 122.7+45 179.4+62 227.8226: 216.8 +61*
Units/mg protein 1.52kO.5 l-78+0.7 2.20+0.2 2.05TO.5
Maltase ??
Units/gm tissue 300.7291 398.1+82 466.0237: 484.3+28,
Units/mg protein 3.36kO.5 4.0320.8 4.5420.6 4.51kO.2
Alkaline Phosphatase
Units/gm tissue 428 +16 313 +9* 532 +20+ 613 +12*
Units/mg protein 4.5520.5 3.33+0.4* 5.4Ok1.5 5.11+0.4
Acid Phosphatase
Units/gm tissue 82.2+20 134.5221: 156.7+24= 125.6+23
Units/mg protein 1.08kO.l 1.3520.2 1.45kO.l 1.03+0.04
LDH
Units/gm tissue 63.8214 70.0+17 87.2+18* 87.2+14*
Units/mg protein 0.8420.1 0.66+0.1* 0.8520.1 0.8320.1
_____---____---_-___~~~~~~~~~~~~~~~~~~~~~~~~~--~~~-~~~~-~-~~~~~~~-~~~~
Mean+S.D., (n=6)
Significantly different from controls tpx0.05.
Table 3.
Total ATPase ??
Units/gm tissue 132.2244.5 98.9228.5, 83.5k13.1, 90.6+15.1;
Units/mg protein l-84+0.61 0.99kO.28 0.80+0.12 0.84kO.77
DISCUSSION
REFERENCES
10. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein
estimation with folin-phenol reagent. J Biol Chem. 1951;
193: 265-275.