AS Biology BYB1 Core Principles 10.

4 Enzymes Test
Name Date Q estion 1 % ' 4 & ( ) * $ Total + ,ra-e
A . '(#4& /*0+0 B . '%#4& /)0+0 C . %)#4& /(0+0 D . %'#4& /&0+0 E . 1*#%& /40+0

!ar"s #1$ #& #4 #' #% #4 #% #4 #% #4&

Enzyme Properties 1. Complete the following paragraphs filling in the missing words (19 marks) energy o4 reactions. T3ey are

Enzymes are 1iological catalysts2 re- cing t3e compose- o4

proteins 5it3 a speci4ic tertiary str ct re.

6n t3e complementary to t3e s3ape o4 t3e acti7e site. 6n t3e acti7e site alters to 4it t3e s 1strate.

3ypot3esis2 t3e s 1strate s3ape is 3ypot3esis t3e

6ncreasing temperat re increases t3e

energy o4 t3e enzyme an- s 1strate .

molec les2 5it3 3ig3 temperat re ca sing t3e enzyme to 1ecome irre7ersi1ly

Enzymes operate in a t3e

p8 range2 5it3 c3anging p8 aro n- t3e enzyme -isr pting .

6ncreasing enzyme concentration ca ses a

increase in t3e rate o4 an enzyme9

catalyse- reaction2 53ereas increasing s 1strate concentration also increases rate 1 t only ntil a is reac3e- 53ere all acti7e sites are .

Some o4 t3e 5or-s in t3is 1o: may 1e se4 l. Acti ation %lo$"lar (inear +arrow Shape of the s"$strate !enat"red &ndirect (ock and key Potential S"$strate Enzyme &nd"ced fit )a*im"m rate Sat"rated ,nsat"rated #i$ro"s 'inetic )inim"m rate Shape of the acti e site -ide

..

!raw a la$elled sketch graph to show how temperat"re affects enzyme acti ity (/ marks)

0.

E*plain in yo"r own words1 with a scientific reason1 why this c"r e shape is prod"ced (2 marks)

2.

!raw a la$elled sketch graph to show how p3 affects enzyme acti ity (0 marks)

/.

E*plain in yo"r own words1 with a scientific reason1 why this c"r e shape is prod"ced. (. marks)

(.

!raw a la$elled sketch graph to show how s"$strate concentration affects enzyme acti ity. (2 marks)

4.

E*plain in yo"r own words1 with a scientific reason1 why this c"r e shape is prod"ced. (. marks)

5.

!raw a la$elled sketch graph to show how enzyme concentration affects enzyme acti ity. (2 marks)

9.

E*plain in yo"r own words1 with a scientific reason1 why this c"r e shape is prod"ced (. marks)

()a*im"m 2/ marks) )ark scheme 1.0 All 5or-s entere- correctly /ma: 1$0 %.0 /ma: &0 1 Enzyme acti7ity 1 '.0 6ncrease- initial rate o4 reaction - e to 3ig3er temperat re ca sing more collisions /10 Platea - e to optim m temperat re /10 1 1 ;all - e to e:cess temperat res ca sing enzymes to -isintegrate ans3ape o4 acti7e site lost /10 9 1ecoming -enat re- /10 /ma: 40 &.0 Bell s3ape- c r7e 9 optim m p8 in t3e centre /10 <o5er at 1ot3 si-es - e to acti7e site s3ape c3ange- - e to c3arge /10 /ma: %0

Temperat re 4.0 /ma: '0 1 Enzyme acti7ity 1

p8 (.0 /ma: 40

1 1 ).0 6ncrease- initial rate - e to more s 1strate a7aila1le to colli-e 5it3 enzymes /10 Platea - e to all acti7e sites 1eing 4ille- /10 /ma: %0

Enzyme acti7ity 1

S 1strate concentration

1 $.0 6ncrease- initial rate - e to more enzymes a7aila1le to colli-e 5it3 s 1strate /10 Platea - e to all acti7e sites 1eing 4ille- /10 /ma: %0 ()a* 2/)

1 *.0 /ma: 40 Enzyme acti7ity 1 1

Enzyme concentration

A . '(#4& /*0+0 B . '%#4& /)0+0 C . %)#4& /(0+0 D . %'#4& /&0+0

E . 1*#%& /40+0