School of Biotechnology Vignan University :: Vadlamudi School of biotechnology has well qualified and experience faculty who are

specialists in the areas of Bioinformatics, Environmental Biotechnology, Plant Biotechnology and Bioprocess Engineering Biochemical Reaction Engineering etc.

The faculty members are actively involved in research activities in the field of their specialization. They have published good number of research papers in International/ National level journals and conferences. The school attributes its success to the creative and innovative outlook of the students. The school encourages students to participate in both Co-Curricular and Extra Curricular activities. Students are encouraged to carry out mini research projects and to present their findings in the symposiums and conferences. The school helps the students to under go industrial training program to get hands on experience at the end of their sixth semester.

Apart from imparting the general subject knowledge to the students and guiding them in their project work (s), the faculty members are encouraged & supported by the management personals, to motivate for their involvement in various thrust areas of research & development programmes at every level to pursue their research orientation.

Management sponsored projects along with project guides( In-Home Projects) : S.No. Guide(s) 1. Dr. R. Bharath Kumar & Mr. D. John Babu 2. Dr. R. Bharath Kumar & Ms. M. Indira 3. Dr. R. Bharath Kumar & Mr. D. Veerendra Project title Hydroponics systems/soil less cultivation Anti microbial screening studies of selected medicinal plant spices Experimental studies on edible mushroom varieties for nutrition value Sponsoring Agency Vignan Year Budget Approved Rs. 3000 Remarks Completed

2008-09

Vignan

2008-09

3000

Completed

Vignan

2008-09

3500

Completed

Kumar 4. Dr. R. Bharath Kumar & Ms. K. Kanaka.S 5. Dr. S. Asha & Ms. K. Kanaka.S 6. Dr. R. Venkatanadh & Ms. M. Indira

Extraction of biological fibers from selected plants and screening of their anti microbial activity Anti microbial screening studies selected medicinal plant species Bioremediation of textile dyes

Vignan

2008-09

3500

Completed

Vignan

2008-09

3000

Completed

Vignan

2008-09

7500

Completed

ii) Brief Summary/Report of Mini Projects: Name of the Guide(s): Dr. R. Venkata Nadh and Ms. M. Indira Title of the Project : BIOREMEDIATION OF TEXTILE DYES

Name of the Students & Year: 1. 2. 3. 4. 5. V. Sunanda (06391A2330) T. Pravallika (06391A2329) G. Preeti (06391A2321) B. Santi Priya (06391A2336) M. Koushik (06391A2317)

Brief Description of the project (regarding problem formulation, goals to be achieved etc): Dyes are coloring compounds being extensively used in almost all industries and the indiscriminate disposal of dye wastewaters poses one of the major problems on the environment. Therefore, their elimination is mandatory using mechanical/ chemical/ biological treatments. Biological method provides a better and an efficient degradation when compared to chemical methods as well as economic & safer in terms of pollution. Some of the earlier studies proved that bioremediation is an efficient method for the removal of dyes. In this study, effluent-containing dye are collected. Different bioremediation procedures are used for its removal and best bioremediation method will be selected. It has been followed by determination of optimum conditions for the removal of dye from effluents. Then the present method efficiency were compared with the already existing methods for the estimation of economical feasibility.

Name of the guide

:Dr. S. Asha & Ms. K.Kanaka.S

Title of the Project:ANTI MICROBIAL SCREENING STUDIES OF SELECTED MEDICINAL IMPORTANT PLANT SPECIES

Name of the students & Year: 1. 2. 3. 4. K. Rani (06391A2322) A. Sravanthi (06391A2304) P. Rajya Lakshmi (06391A2320) S. Srilakshmi (06391A2326)

Brief Description of the project achieved etc):

(regarding problem formulation, goals to be

Medicinal plants are represents a rich source for which antimicrobial agents may be obtained. Plants are used medicinally agents may be obtained. Plants are used medicinally in different countries and a source of many plant & powerful drugs. The interest in the scientific investigation of these 50 medicinal plants from Nigeria is based on the claims of their effective use for the treatment of many diseases. Therefore, research into the effects of these local medicinal plants in expected to enhance the use of these plants against diseases caused by the test pathogens. However, most of these plants used in folk medicine have not been screened for their antimicrobial activity. The solutions are extracted from methanol & acetone by adding 50 ml for each plant sample/s Tacoma stanns Azadirachta indica leaves, stem bark, flowers leaves, stem bark leaves, stem bark, flowers leaves, stem bark

Allmanda cathertica Pisonia alba -

Name of the guide(s) :Dr. R. Bharath Kumar & Mr. D. Veerendra Kumar Title of the project :EXPERIMENTAL STUDIES ON AGARICUS COMPESTRIS - AN EDIBLE MUSHROOM VARITIE/S FOR ITS NUTRITIONAL COMPOSITION

Name of the students & Year: 1. 2. 3. 4. B.L. Sarojini (06391A2307) B. Hema Lakshmi (06391A2308) D. Jaswanth Chowdary (06391A2311) P. Avinash (06391A2305) (regarding problem formulation, goals to be

Brief Description of the project achieved etc):

Mushrooms as foodstuff were used by the ancient Greek and Romans. The ancient Romans considered mushrooms, as divine flash. The edible mushrooms are valuable sources of nutrients, subsequently contain proteins equal to the value of animal protein. World production of mushroom is 4 lakh tones per anum and is expected to increase at a rate of 10% per anum. Taiwan is a major mushroom producing and exporting country, produces more than 60,000 tonnes of mushroom per year in the rural sector and mushroom cultivation is a highly mechanized industry. Agaricus compestris [temperature mushroom] and Valvariella volvacca (tropical mushroom, are cultivated artificially through out the world. The edible nature of mushroom is known from time international. Romans considered them as Food of Gods. Historically mushrooms have been equated to vegetable beef steak. Mushrooms contain nutrients in 100 gm of edible portion, viz, 35gm of protein, 0.4 gm of fat, 1.2 gm of ash, 4.0 gm of carbohydrate, 0.9 gm of crude fibre, 9 mg of calcium, 115 mg of sodium, 520 mg of potassium, 0.2 mg of thiamine, 0.52 mg of riboflavin, 8.60 mg of ascorbic acid, 5.85 mg of niacin, 2.38 mg of pantothenic acid, 29 mg of folic acid, 16 mg of biotin, 90 mg of moisture and 16 calories. Mushrooms are considered as vegetarian diets. There are over 250 different varieties of edible mushrooms which serve as valuable sources of nutrients and available all the year round. The existence of Proteins, Amino acids, Fats, Carbohydrates and Fibre has been observed qualitative tests in Edible mushrooms.

Name of the guide(s) :Dr. R. Bharath Kumar & Ms. K.Kanaka.S Title of the project : EXTRACTION OF BIOLOGICAL FIBRES FROM SELECTED PLANTS & SCREENING OF THEIR ANTIMICROBIAL ACTIVITIES

Name of the Students & Year: 1. A. Vasavi (06391A2302) 2. G. Sarada (06391A2313) 3. D. Sireesha (07391A2302)

Brief Description of the project achieved etc):

(regarding problem formulation, goals to be

Typha angustifolia Linn. a common plant of wetlands, is an unexploited taxon which can be used as a good source of food, medicines and fibres. Agave cantala Roxb. A commonly cultivated worldwide as an ornamental plant. It has since naturalised in many regions and grows wild in Europe, South Africa, Australia, New Zealand and in India. They have been also identified as, few of the most promising domestic, commercial fibre yielding plants from the ancient literature sources (Anonymous 194876, Chopra, R.N. S.L. Nayar & I.C. Chopra 1956). The plants, which are selected, are collect & these are grinded by motor & pestle, the suspension liquid is collected. Simultaneously different types of fungus & bacteria are cultured in the media. The paper disks are dipped in the suspension. These paper disks are kept in culture media, where microbes are grown & left to 24-36hrs. The inhibition zones are developed. A total no of 24 methanol and acetone soxhlet extracts from the 2 plant species Twenty four samples exhibited positive activity . Out of these 24samples 6 species only have exhibited cognizable inhibitory zone (>15mm.) i.e 16% in the total of test samples. (24 plant extracts)

The inhibitory activity of methanol extracts is comparatively more than those of acetone extracts. The methanol and acetone extracts are shown high inhibitory activity in Gram +ve bacteria and comparatively less in both Gram ve bacteria. 24 samples when subjected to inhibition activity test 14 samples, are positive for inhibition rests of the 4 samples are passive. Details are as follows. Immunity response of species against microbial activity as per pharmaceutical categories is as follows. -A/M 8 : 3 sps : A/M 9 & 12 : 4sps :A/M 10 & 15 : 6 sps

A/M: 11,14 ,19 & 20 : 10 sps. Upon critical study and to summaries the antimicrobial activity test, it is noticed that out of 24samples with positive response the cognizable inhibition could be observed i.e, above 20 mm in 4 species only. 2 species have exhibited immunity zone against G +ve bacteria. Expression for inhibition is either for both test elements vig. Bacteria or one element ie. Bacteria the solvent extracts in A/M (Acetone / Methanol). In all two only. Out of 24 plant extract samples, have exhibited cognizable inhibitory zone (20mm) i.e. 16-17% of the total test samples. Antibacterial inhibitory expression is observed in extracts of both in Typha angustifolia, Agave cantala M/A solvents respectively. Regarding antibacterial inhibition, 4 species are positive with cognizable expression. Thus only 16% of the total samples are positive for antimicrobial immunity. These show the antimicrobial property of the collected plant fibers.

Name of the guide Title of the Project

:Dr. R. Bharath Kumar & Mr. D. John Babu :HYDROPONIC SYSTEMS / SOIL LESS CULTURE.

Name of the Students & Year: 1. 2. 3. 4. 5. R. Devi Chowdary (06391A2323) V. Anusha (06391A2331) A. Sindhura (06391A2301) A. Srilatha (06391A2303) S. Sahul Phani (06391A2327)

Brief Description of the project achieved etc):

(regarding problem formulation, goals to be

Hydroponics is the science of growing plants in a cesium other than soil, using mixtures of essential of nutrient elements dissolved in water. The term hydroponics derived from too Greek olds Hydro (water) and ponos (labour) was carried by Dr. WF. Gericke of California. He used the ten hydroponics for growing the crop plants in aqueous nutrient salts. In such culture the soil is not needed as a medium and therefore it is commonly known as soil less culture. The culture is carried out under sufficient temperature; light and constant pH should also be maintained. The soilless culture is chiefly of 3 types. They are

1. 2. 3.

Water culture Sand culture Gravel culture

Hydroponics systems or soil less cultures is used to grow healthy plants in a limited space, to minimize the soil-bone diseases, to produce plants within a short time. In this process we can produce plants soil by giving them required nutrients and water. We can produce plantlets within short time and space. Now a days, this process mainly

used for the horticulture techniques and for the cultivation of Indoor plants. In trial II nutrient vocational were observed in each solution one moment was not added so that the deficiency were observed in the plants. During the nutrient variations Rice plant affected Earlike than other plants. Trial I & Trial II were conducted simultaneously the plants which were grown in trail I has long life than that of the plants which were grown in trail II. In the trail III the plants taken were tomato, chilies, chrysanthemum. All the plants were dried except the chillis in the solution where boric acid was not present.

Name of the guide Title of the Project

:Dr. R. Bharath Kumar & Ms.M. Indira :ANTIMICROBIAL SCREENING STUDIES OF CERTAIN SELECTED MEDICINALLY IMPORTANT PLANT SPECIES

Name of the Students & Year: 1. 2. 3. 4. M. Aditya Ramesh (06391A2318) R. Krishna Kishore (06391A2316) N. Sailesh (06391A2334) S. Phanindra (06391A2325)

Brief Description of the project achieved etc):

(regarding problem formulation, goals to be

Medicinal plants are represents a rich source for which antimicrobial agents may be obtained As the science and technology is increasing the depending on plants and Animals also increasing plants plays a vital role in synthesis of anti microbial drugs. In present project we will discuss about different plant spices like Calotropis gigantea, Ocimum tenuiflorm, Mangifera indica, Catharathees roseus and Acorus calamus which show anti microbial properties. At first required plant species are educated and washed The collected plant parts are dried and grinded properly until fine particles size All the glass ware and apparatus are sterilized properly Each plant part which grinded are weighed to 1 gm and taken in different test tubes Each sample is with two different solvents i.e., action, methanol separately These are mixed thoroughly by cyclometer and kept aside for one night PDA media and nutrient Agar media are prepared and poured into petriplates next day Gram+ve bacterial bacillus and Gram-ve bacteria E.coli are inoculated to nutrient Agar media and fungus Phytopthera sp. is inoculated to PDA media

The supernatant is collected into another test tubes and paper disks are soaked into it for 6 hrs

Later these paper discs are transferred into petriplates by using forceps in laminar flow chamber.

These petriplates containing bacteria are incubated for 24hrs & fungus is incubated for 48hrs

After incubation we will observe a inhibition area around paper discs By using scale the inhibition area is measure and tabulated. The inhibition zones are observed around all the samples we have taken. By this we can conclude that the samples, which we have taken are having

antimicrobial properties.