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lebruary 2014
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Section 1
Lecuve wrlung
Active voice
Sentences wr|uen |n the acnve vo|ce are:
32,61+ *2&+(# (1+)& +)3- #% &+)*
1he mechan|sms regu|anng tumor growth were |nvesngated.
>)332=+
We |nvesngated the mechan|sms regu|anng tumor growth.
!(<=+
Lecuve wrlung
Active voice
70
73
80
83
90
93
100
Acuve asslve
Accuracy ()
1300
1600
1700
1800
1900
2000
2100
Acuve asslve
Dec|s|on nme (ms)
lerrelra l. Cognluve sychology 2003, 47: 164-203.
48 nauve Lngllsh-speaklng unlverslLy sLudenLs
36 acuve volce senLences ('Lhe dog blL Lhe man')
36 passlve volce senLences ('Lhe man was bluen by Lhe dog')
Lecuve wrlung
Active voice is preferred
use Lhe )(<=+ =%2(+ when lL ls less wordy and more
dlrecL Lhan Lhe passlve". (3
rd
ed., pg. 42)
use Lhe )(<=+ =%2(+ raLher Lhan Lhe passlve volce.".
www.apasLyle.org/learn/faqs/eecuve-verb-use.aspx
As a mauer of sLyle, passlve volce ls Lyplcally, buL noL
always, lnferlor Lo )(<=+ =%2(+". (13
Lh
ed., pg. 177)
ln general, auLhors should use Lhe )(<=+ =%2(+.".
(10
Lh
ed., pg. 320)
!?@ @#-1+ A"2*+
!>! @#-1+
?$2()0% @#-1+
A"2*+
!B! B)4")1 %C
@#-1+
Lecuve wrlung
Stress position
D+)*+&3 C%("3 )# #$+ +4* %C #$+ 3+4#+4(+ #%
*+#+&,24+ 8$)# 23 2,6%&#)4#E
1. ou deserve a ra|se, but the budget |s nght.
Wh|ch sentence suggests that you
8211 get a ra|se?
2. 1he budget |s nght, but you deserve a ra|se.
hup://wrlungcenLer.unc.edu/handouLs/ow/
Lecuve wrlung
1he budget |s nght, but you deserve a ra|se. our sa|ary
w||| |ncrease at the beg|nn|ng of next year.
hup://wrlungcenLer.unc.edu/handouLs/ow/
Stress pos|non 1op|c pos|non
F$+ #%62( 6%32<%4 24#&%*"(+3 #$+ 2*+) %C #$+ ("&&+4# 3+4#+4(+
1he stress pos|non a|so |ntroduces
the top|c of the next sentence
Stress position
Lecuve wrlung
Topic position
F$+ 6)<+4# went to the hosp|ta| to see a
0)3#&%+4#+&%1%023#. F$+ *%(#%& then performed a
ser|es of *2)04%3<( #+3#3. F$+ &+3"1#3 showed the
panent suered from a 5)(#+&2)1 24C+(<%4. !4<52%<(3
were prescr|bed to treat the |nfecnon before the
panent deve|oped an "1(+&.
ldea ldea ldea ldea
1oplc llnk
senLence
Lecuve wrlung
8usch eL al. 8MC Cancer. 2012, 13: 211.
Linking your ideas in
your manuscript
Lung cancer ls Lhe leadlng cause of cancer morLallLy for men and women.
uesplLe smoklng prevenuon and cessauon programs and advances ln early
deLecuon, Lhe 3-year survlval raLe for lung cancer ls only 16 wlLh currenL
Lheraples. AlLhough lung cancer lncldence raLes have recenLly decllned ln Lhe
unlLed SLaLes, more lung cancer ls now dlagnosed when consldered LogeLher ln
former- and never-smokers Lhan ln currenL smokers. 1hus, even lf all of Lhe
nauonal anu-smoklng campalgn goals are meL, lung cancer wlll remaln a ma[or
publlc healLh problem for decades. new ways Lo LreaL or prevenL lung cancer
are Lherefore needed.

Cne poLenual Lherapeuuc LargeL for lung cancer ls Lhe WnL slgnallng paLhway.
1he canonlcal WnL slgnallng paLhway ln mammals conslsLs of a famlly of
secreLed llpld-modled WnL proLeln llgands LhaL blnd Lo a famlly of 7-pass
Lransmembrane lrlzzled (lzd) recepLors, as revlewed.
Lecuve wrlung

1op|c sentence
your manuscript
Lecuve wrlung

1op|c sentence
Stress sentence
your manuscript
Lecuve wrlung

1op|c sentence
Stress sentence
1op|c sentence
G3+ #$+ #%62( )4* 3#&+33 6%32<%43 #% 1%02()11-
6&+3+4# -%"& 2*+)3 82#$24 -%"& ,)4"3(&26#
your manuscript
B)4"3(&26# 3#&"(#"&+
Section 2
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Introduction
Genera| |ntroducnon
Spec|hc a|ms
Alms
Current state of the he|d
rob|em |n the he|d
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Introduction
flow of information
Lung cancer ls Lhe leadlng cause of cancer morLallLy for men and women. uesplLe smoklng prevenuon and cessauon programs and advances ln early
deLecuon, Lhe 3-year survlval raLe for lung cancer ls only 16 wlLh currenL Lheraples. AlLhough lung cancer lncldence raLes have recenLly decllned ln Lhe
unlLed SLaLes, more lung cancer ls now dlagnosed when consldered LogeLher ln former- and never-smokers Lhan ln currenL smokers. 1hus, even lf all of Lhe
nauonal anu-smoklng campalgn goals are meL, lung cancer wlll remaln a ma[or publlc healLh problem for decades. new ways Lo LreaL or prevenL lung cancer

Cne poLenual Lherapeuuc LargeL for lung cancer ls Lhe WnL slgnallng paLhway. 1he canonlcal WnL slgnallng paLhway ln mammals conslsLs of a famlly of
secreLed llpld-modled WnL proLeln llgands LhaL blnd Lo a famlly of 7-pass Lransmembrane lrlzzled (lzd) recepLors, as revlewed. ln brlef, ln Lhe absence of
llgand, glycogen synLhase klnase-3 (CSk3), ln complex wlLh axln and adenomaLous polyposls coll (AC), consuLuuvely phosphorylaLes -caLenln, Lhe prlmary
WnL slgnallng eecLor, Largeung lL for ublqulunauon and proLeasomal desLrucuon. Llgand blndlng engages a paLhway lnvolvlng ulshevelled (uvl) LhaL
lnhlblLs CSk3, allowlng -caLenln Lo accumulaLe ln a hypophosphorylaLed form. 1hls sLablllzed form of -caLenln can LranslocaLe Lo Lhe nucleus, where lL
acuvaLes LargeL gene Lranscrlpuon by complexlng wlLh 1 cell facLor (1Cl) and lymphold enhancer-blndlng facLor (LLl). ln addluon Lo key medlaLors of
embryonlc developmenL, Lhese LargeL genes lnclude crlucal growLh-regulaLors such as myc and cycllo u1.

AberranL WnL slgnallng due Lo muLauons ln 6-coteolo or Alc drlves deregulaLed growLh ln boLh famlllal and non-heredlLary colorecLal cancers. Powever,
non-small cell lung cancers (nSCLC), Lhe mosL common Lype of lung cancer, rarely harbor Alc or 6-coteolo muLauons. 8aLher, aberranL WnL acuvlLy ln lung
cancer ls llnked Lo lncreased expresslon of upsLream WnL slgnallng eecLors such as uvl or decreased expresslon of WnL anLagonlsLs such as WnL-lnhlblLory
facLor 1 (Wlf-1).

Lecuve pharmacologlcal lnhlblLors of Lhe WnL paLhway have only recenLly become avallable. Screens for small-molecule anLagonlsLs of Lhe WnL paLhway
found Lwo enzymes Lo be key medlaLors of WnL slgnallng. 1hese are poly-Au-rlbose polymerase (A8) enzymes, Lankyrase (1nkS) 1 and 1nkS2, whlch
auach poly-Au-rlbose (A8) onLo subsLraLe proLelns. 1helr roles ln regulaung Lelomerase funcuon and mlLouc splndle formauon are known, buL Lhelr role
ln A8sylaung axln so as Lo malnLaln Lhe opumal level for canonlcal WnL slgnallng has only recenLly been recognlzed. 1he compounds ldenued ln Lhese
screens, xAv939, lW8-1 exo, and lW8-1 endo, acL by speclcally lnhlblung Lhe A8 acuvlLy of 1nkS1 and 1nkS2. lW8-exo ls a sLereolsomer of lW8-1 endo
wlLh ~14-fold lower LC
30
. A8 lnhlbluon ls a LracLable pharmacologlcal LargeL lo vlvo, as anLagonlsLs of oLher A8 homologs exerL anuneoplasuc
responses ln breasL and ovarlan cancer, as revlewed.

1hls sLudy explored Lhe hypoLhesls LhaL lnhlbluon of 1nkS by pharmacologlcal or geneuc means would lnhlblL lung cancer growLh lo vltto and lo vlvo ln
cllnlcally-relevanL Lransgenlc mouse models of lung cancer LhaL were prevlously developed, as revlewed.

Genera| |ntroducnon
Secondary |ntroducnon
Cb[ecnves
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Writing the Introduction
H+0244240 should demonsLraLe
relevance/lnLeresL
Lung cancer ls Lhe leadlng cause of cancer morLallLy for men and women. uesplLe
smoklng prevenuon and cessauon programs and advances ln early deLecuon, Lhe 3-year
survlval raLe for lung cancer ls only 16 wlLh currenL Lheraples. AlLhough lung cancer
lncldence raLes have recenLly decllned ln Lhe unlLed SLaLes, more lung cancer ls now
dlagnosed when consldered LogeLher ln former- and never-smokers Lhan ln currenL
smokers. 1hus, even lf all of Lhe nauonal anu-smoklng campalgn goals are meL, lung
cancer wlll remaln a ma[or publlc healLh problem for decades. new ways Lo LreaL or
prevenL lung cancer are Lherefore needed.
Interest
I,6%&#)4# 6&%51+, 24 #$+ J+1*
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
?our )2,3 should dlrecLly
address Lhls problem
1hls sLudy explored Lhe hypoLhesls LhaL lnhlbluon of 1nkS by
pharmacologlcal or geneuc means would lnhlblL lung cancer growLh lo vltto
and lo vlvo.
Writing the Introduction
New ways to treat or prevent |ung cancer are therefore needed.
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Methods
now |t was
done
Genera| methods
Spec|hc techn|ques
(d|scuss contro|s)
Data ana|ys|s
uannhcanon methods
Stansnca| tests
What was
used
Samp|es or parnc|pants
Mater|a|s
now |t was
ana|yzed
:K6+&2,+4#)1 L+3204
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Results
1. In|na| observanon
2. Character|zanon
3. App||canon
Log|ca| presentanon
:K),61+.
1. New gene expressed |n the heart
2. kegu|anon of gene express|on, when |t |s
expressed, funcnon of the produced prote|n
3. ko|e of the gene |n heart deve|opment
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Results
1. In|na| observanon
2. Character|zanon
3. App||canon
Lach subsecnon
corresponds to
%4+ hgure
What you found, 4%#
what |t means
Log|ca| presentanon
Subsecnons
Iactua| descr|pnon
Coverage and
SLamng lan
ManuscrlpL
sLrucLure Display items
resent |arge amount
of data M"2(N1- and
+O(2+4#1-
keep |t 32,61+: use
separate pane|s |f
necessary
Must be ab|e to 3#)4*
)1%4+: c|ear |abe|s
and hgure |egends
Usua||y the J&3# th|ng
readers w||| |ook at
I|gures, graphs
& tab|es
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Figures
C|ear hgure |egend
k|nd||n-2 knockdown and foca| adhes|on |oca||zanon.
Confocal lmmunouorescenL mlcroscopy wlLh anu-1
lnLegrln and anu-paxlllln on C2C12 cells LransfecLed
wlLh 8nAl and Lhen changed Lo dlerenuauon medla
for 2 days. ConLrol cells show llnear sLalnlng conslsLenL
wlLh locallzauon Lo cosLameres (arrows), as well as
puncLaLe focal conLacL sLalnlng (arrowheads). local
conLacL proLelns ln Lhe klndlln-2 8nAl cells fall Lo form
llnear sLrucLures and lnsLead are concenLraLed ln
unusual appearlng puncLa (*). (Scale bar = 20 M).
uowllng eL al. (2008) 8MC Cell 8lol 9:36.
C|ear |nd|cators
1|t|e of the exper|ment
8r|ef methodo|ogy
key hnd|ngs
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Discussion
Summary of hnd|ngs
ke|evance of
hnd|ngs
Imp||canons for
the he|d
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
CL8 ls an L
2
blndlng, C-proLeln coupled membrane recepLor LhaL was reporLed Lo be overexpressed ln breasL endomeLrlal, ovarlan and Lhyrold cancers. 1he
resulLs presenLed here exLend Lhese observauons Lo show LhaL dlerenL Lypes of lung cancers lncludlng adenocarclnomas, squamous cell carclnoma and large
cell carclnomas express hlgher CL8 Lhan normal lung ussue. Pere, we demonsLraLe for Lhe rsL ume LhaL CL8 ls overexpressed ln lung Lumors and lung
adenocarclnoma cell llnes relauve Lo normal lung and lmmorLallzed normal lung cell llnes, alLhough Lhe expresslon of CL8 LranscrlpL ln PL1u cells ls hlgher
Lhan P8LCs.

CL8 has been posLulaLed Lo be lnvolved ln L
2
-acuvauon of LCl8. lllardo's group showed a llnk beLween CL8 expresslon and Lumor progresslon and lncreased
Lumor slze ln breasL cancer pauenLs. 8ecenLly, CL8 overexpresslon was reporLed Lo be lndependenL of L8o expresslon ln breasL cancer pauenL samples,
lndlcaung Lhe lmporLance of CL8 ln L8o negauve Lumors. CL8 and LCl8 expresslon were correlaLed ln endomeLrlal adenocarclnoma. lurLher, overexpresslon
of CL8 ln advanced sLage endomeLrlal adenocarclnoma correlaLed wlLh poor survlval. CLher sLudles also suggesL lncreased CL8 ln breasL, ovarlan and
endomeLrlal cancers correlaLes wlLh dlsease severlLy and reduced survlval. 1hese resulLs are ln agreemenL wlLh sLudles demonsLraung assoclauon of CL8
overexpresslon ln oLher cancers, alLhough Lhe scorlng pauerns and correlauon of expresslon levels Lo dlsease sLaLe may vary among Lhese sLudles.

A llmlLauon of our sLudy ls LhaL Lhe average CL8 sLalnlng scores among dlerenL lung cancer grades (l (10 cases), ll (30 cases), lll (16 cases)) were noL
slgnlcanLly dlerenL. Cne oLher llmlLauon of Lhe currenL sLudy ls LhaL we cannoL conclude aL Lhls ume wheLher CL8 overexpresslon ls cause or consequence of
cancer. lL ls also posslble LhaL overexpresslon of CL8 ln lung cancers may reecL a defense mechanlsm Lo counLeracL excesslve prollferauon. lndeed, a recenL
reporL by kraksLad et ol. showed LhaL loss of CL8 ln L8o-posluve endomeLrlal cancers ls assoclaLed wlLh poor prognosls. AnoLher sLudy showed LhaL Lhe CL8
agonlsL C-1 lnhlblLed L
2
-lnduced uLerlne eplLhellal cell prollferauon ln mlce by represslng MAk acuvauon, lndlcaung LhaL CL8 eecLs are ussue speclc.
8ecause our sLudles were performed on commerclal 1MAs, Lhe resulLs cannoL be exLrapolaLed Lo correlaLe CL8 expresslon levels Lo dlsease ouLcomes. Clearly,
Lhls ls a nexL loglcal sLep ln llghL of Lhe novel ndlngs.

We observed no dlerences ln CL8 expresslon beLween adenocarclnoma cell llnes or Lumors from male and female pauenLs, slmllar Lo Lhe prevlous ndlngs of
no dlerence ln L8o or L8 expresslon ln nSCLC cells and Lumors based on gender. ln WesLern bloLs, raLher Lhan rely on one CL8 anubody ln our sLudy, we
used 3 dlerenL commerclal anubodles Lo deLermlne Lhe correlauon beLween m8nA and proLeln levels. lL ls lndeed evldenL from our WesLern bloL daLa LhaL
CL8 appears Lo have dlerenL MW forms, llkely due Lo glycosylauon, dlmerlzauon, and lnLeracuon wlLh oLher membrane proLelns, and levels ln Lhe lung
adenocarclnoma cell llnes. More Lrlvlal explanauons for Lhe dlerenL sLalnlng pauerns of CL8 ln WesLern bloLs may be due Lo dlerenual purlLy/amnlLy of Lhe
Lhree CL8 anubodles as well as Lhelr capaclLy Lo blnd Lo secondary anubodles. lL wlll be lmporLanL Lo deLermlne Lhe naLure of Lhese forms by proLeomlc analysls
and gene sequenclng Lo evaluaLe Lhelr blologlcal slgnlcance.

Mechanlsm-based sLudles showed LhaL CL8 LransacuvaLes LCl8 ln breasL cancer cells as well as ln Lhyrold, endomeLrlal and ovarlan cancer cell llnes. lnhlblLors
of LCl8 Lyroslne klnase (geunlb) and L8 (fulvesLranL, lCl 182,780) were reporLed Lo synerglze Lhelr anu-prollferauve eecLs ln nSCLC . Clven Lhe lmporLance of
LCl8 slgnallng as a Lherapeuuc LargeL ln lung cancer, furLher examlnauon of Lhe eecL of LCl, heregulln, and amphlregulln on CL8 expresslon and funcuon ln
lung cancer may provlde new lnslghLs lnLo reslsLance Lo LCl8 lnhlblLors and or how esLrogens sumulaLe lung cancer.

ln concluslon, Lhe daLa presenLed ln Lhls manuscrlpL demonsLraLe LhaL CL8 expresslon ls hlgher ln lung Lumors compared Lo normal lung ussue. Whlle lL ls noL
yeL clear LhaL elevaLed CL8 expresslon ls a cause of or consequence from lung cancer progresslon. luncuonal analysls of Lhe eecL of CL8 expresslon wlll
faclllLaLe furLher dellneauon of Lhe role of CL8 ln lung cancer.

8ao !ala eL al. 8Mc coocet 2012,12:624.
Discussion
flow of information
Summary
Ma[or conc|us|on
8elevance
rev|ous stud|es
L|m|tanons
Unexpected resu|ts
Concluslons
Imp||canons
Ma[or conc|us|on
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
CL8 ls an L
2
blndlng, C-proLeln coupled membrane recepLor LhaL was reporLed Lo
be overexpressed ln breasL, endomeLrlal, ovarlan and Lhyrold cancers. 1he resulLs
presenLed here exLend Lhese observauons Lo show LhaL dlerenL Lypes of lung
cancers lncludlng adenocarclnomas, squamous cell carclnoma and large cell
carclnomas express hlgher CL8 Lhan normal lung ussue. Pere, we demonsLraLe
for Lhe rsL ume LhaL CL8 ls overexpressed ln lung Lumors and lung
adenocarclnoma cell llnes relauve Lo normal lung and lmmorLallzed normal lung
cell llnes, alLhough Lhe expresslon of CL8 LranscrlpL ln PL1u cells ls hlgher Lhan
P8LCs.
Discussion
flow of information
H+0244240 should sLaLe Lhe ma[or
concluslon of Lhe sLudy
8e-lnLroducuon
Concluslon
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
ln concluslon, Lhe daLa presenLed ln Lhls manuscrlpL demonsLraLe LhaL CL8
expresslon ls hlgher ln lung Lumors compared Lo normal lung ussue. Whlle lL ls noL
yeL clear LhaL elevaLed CL8 expresslon ls a cause of or consequence from lung
cancer progresslon. luncuonal analysls of Lhe eecL of CL8 expresslon wlll
faclllLaLe furLher dellneauon of Lhe role of CL8 ln lung cancer.
Discussion
flow of information
:4* should summarlze your concluslon
and clearly sLaLe Lhe lmpllcauons
8e-sLaLe concluslon lmpllcauons
luLure dlrecuons
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Linking your ideas
Genera| background
Cb[ecnves
Methodo|ogy
kesu|ts and hgures
Summary of hnd|ngs
Imp||canons for the he|d
ke|evance of hnd|ngs
rob|ems |n the he|d
P%02()11- 124N -%"& 2*+)3 #$&%"0$%"# -%"& ,)4"3(&26#
Current state of the he|d
Introducnon
Methods
kesu|ts
D|scuss|on
Coverage and
SLamng lan
ManuscrlpL
sLrucLure
Linking your ideas
New ways to treat or prevent |ung cancer
are therefore needed.
1h|s study exp|ored the hypothes|s that |nh|b|non
of 1NkS.wou|d |nh|b|t |ung cancer growth.
harmaco|og|ca| or genenc |nh|b|non of 1NkS1
and 1NkS2.reduces |ung cancer pro||feranon...
rob|em
Cb[ecnves
Conc|us|on
L23("332%4
I4#&%*"(<%4
8usch eL al. 8Mc coocet. 2012,13:211.
F2#1+3 )4* )53#&)(#3
Section 3
CusLomer Servlce
1lLles and absLracLs
Important po|nts
! Summar|ze key hnd|ng
! Conta|ns keywords
! Less than 20 words
Avo|d
Effective titles
/%"& <#1+ 3$%"1* 5+ ) (%4(23+ 3",,)&- %C
-%"& ,%3# 2,6%&#)4# J4*240
uesnons
Abbrev|anons
"New" or "nove|"
CusLomer Servlce
Abstract
I|rst |mpress|on
of your paper
Importance of
your resu|ts
Va||d|ty of your
conc|us|ons
ke|evance of
your a|ms
Iudge your
wr|nng sty|e
robab|y on|y part
that w||| be read
CusLomer Servlce
Sections of an abstract
A|ms
8ackground
Methods
kesu|ts
Conc|us|on
Why the study was done (20)
our hypothes|s (10)
1echn|ques (10)
Most |mportant hnd|ngs (40)
Conc|us|on]|mp||canons (20)
?%4(23+ 3",,)&- %C -%"& &+3+)&($
CusLomer Servlce
Unstructured abstract
Cur undersLandlng of Lhe mechanlsms by whlch ducLs and lobules develop ls derlved from
model organlsms and Lhree-dlmenslonal (3u) cell culLure models whereln mammallan
eplLhellal cells undergo morphogenesls Lo form mulucellular spheres wlLh a hollow cenLral
lumen. Powever, Lhe mechanophyslcal properues assoclaLed wlLh eplLhellal morphogenesls
are poorly undersLood. We performed muludlmenslonal llve-cell lmaglng analysls Lo Lrack Lhe
morphogeneuc process sLarung from a slngle cell Lo Lhe developmenL of a mulucellular,
spherlcal sLrucLure composed of polarlzed eplLhellal cells surroundlng a hollow lumen. We
reporL LhaL ln addluon Lo acuvely malnLalnlng aplcobasal polarlLy, Lhe sLrucLures underwenL
roLauonal mouons aL raLes of 13-20 m/h and Lhe sLrucLures roLaLed 360 every 4 h durlng
Lhe early phase of morphogenesls. 8oLauonal mouon was lndependenL of Lhe cell cycle, buL
was blocked by loss of Lhe eplLhellal polarlLy proLelns Scrlbble or ard3, or by lnhlbluon of
dyneln-based mlcroLubule moLors. lnLeresungly, none of Lhe sLrucLures derlved from human
cancer underwenL roLauonal mouon. We found a dlrecL relauonshlp beLween roLauonal
mouon and assembly of endogenous basemenL membrane maLrlx around Lhe 3u sLrucLures,
and LhaL sLrucLures LhaL falled Lo roLaLe were defecuve ln weavlng exogenous lamlnln maLrlx.
ulssoluuon of basemenL membrane around maLure, nonroLaung aclnl resLored roLauonal
movemenL and Lhe ablllLy Lo assemble exogenous lamlnln. 1hus, coordlnaLed roLauonal
movemenL ls a unlque mechanophyslcal process observed durlng normal 3u morphogenesls
LhaL regulaLes lamlnln maLrlx assembly and ls losL ln cancer-derlved eplLhellal cells.
Wang eL al. nAS. 2013, 110: 163-168.
CusLomer Servlce
Unstructured abstract
Conc|us|on
1hus, coordlnaLed roLauonal movemenL ls a unlque mechanophyslcal process observed durlng normal
3u morphogenesls LhaL regulaLes lamlnln maLrlx assembly and ls losL ln cancer-derlved eplLhellal cells.
kesu|ts
We reporL LhaL ln addluon Lo acuvely malnLalnlng aplcobasal polarlLy, Lhe sLrucLures underwenL
roLauonal mouons aL raLes of 13-20 m/h and Lhe sLrucLures roLaLed 360 every 4 h durlng Lhe early
phase of morphogenesls. 8oLauonal mouon was lndependenL of Lhe cell cycle, buL was blocked by loss
of Lhe eplLhellal polarlLy proLelns Scrlbble or ard3, or by lnhlbluon of dyneln-based mlcroLubule
moLors. lnLeresungly, none of Lhe sLrucLures derlved from human cancer underwenL roLauonal mouon.
We found a dlrecL relauonshlp beLween roLauonal mouon and assembly of endogenous basemenL
membrane maLrlx around Lhe 3u sLrucLures, and LhaL sLrucLures LhaL falled Lo roLaLe were defecuve ln
weavlng exogenous lamlnln maLrlx. ulssoluuon of basemenL membrane around maLure, nonroLaung
aclnl resLored roLauonal movemenL and Lhe ablllLy Lo assemble exogenous lamlnln.
Methods
We performed muludlmenslonal llve-cell lmaglng analysls Lo Lrack Lhe morphogeneuc process sLarung
from a slngle cell Lo Lhe developmenL of a mulucellular, spherlcal sLrucLure composed of polarlzed
eplLhellal cells surroundlng a hollow lumen.
8ackground
Cur undersLandlng of Lhe mechanlsms by whlch ducLs and lobules develop ls derlved from model
organlsms and Lhree-dlmenslonal (3u) cell culLure models whereln mammallan eplLhellal cells undergo
morphogenesls Lo form mulucellular spheres wlLh a hollow cenLral lumen. Powever, Lhe
mechanophyslcal properues assoclaLed wlLh eplLhellal morphogenesls are poorly undersLood.
Wang eL al. nAS. 2013, 110: 163-168.
CusLomer Servlce
Writing your abstract
organlsms and Lhree-dlmenslonal (3u) cell culLure models whereln mammallan eplLhellal cells
undergo morphogenesls Lo form mulucellular spheres wlLh a hollow cenLral lumen. Powever, Lhe
mechanophyslcal properues assoclaLed wlLh eplLhellal morphogenesls are poorly undersLood. We
performed muludlmenslonal llve-cell lmaglng analysls Lo Lrack Lhe morphogeneuc process sLarung
eplLhellal cells surroundlng a hollow lumen. We reporL LhaL ln addluon Lo acuvely malnLalnlng
aplcobasal polarlLy, Lhe sLrucLures underwenL roLauonal mouons aL raLes of 13-20 m/h and Lhe
sLrucLures roLaLed 360 every 4 h durlng Lhe early phase of morphogenesls. 8oLauonal mouon was
lndependenL of Lhe cell cycle, buL was blocked by loss of Lhe eplLhellal polarlLy proLelns Scrlbble or
ard3, or by lnhlbluon of dyneln-based mlcroLubule moLors. lnLeresungly, none of Lhe sLrucLures
derlved from human cancer underwenL roLauonal mouon. We found a dlrecL relauonshlp beLween
roLauonal mouon and assembly of endogenous basemenL membrane maLrlx around Lhe 3u
sLrucLures, and LhaL sLrucLures LhaL falled Lo roLaLe were defecuve ln weavlng exogenous lamlnln
maLrlx. ulssoluuon of basemenL membrane around maLure, nonroLaung aclnl resLored roLauonal
movemenL and Lhe ablllLy Lo assemble exogenous lamlnln. 1hus, coordlnaLed roLauonal movemenL
ls a unlque mechanophyslcal process observed durlng normal 3u morphogenesls LhaL regulaLes
lamlnln maLrlx assembly and ls losL ln cancer-derlved eplLhellal cells.
Wang eL al. nAS. 2013, 110: 163-168.
CusLomer Servlce
Writing your abstract
organlsms and Lhree-dlmenslonal (3u) cell culLure models whereln mammallan eplLhellal cells
undergo morphogenesls Lo form mulucellular spheres wlLh a hollow cenLral lumen. Powever, Lhe
mechanophyslcal properues assoclaLed wlLh eplLhellal morphogenesls are poorly undersLood. We
performed muludlmenslonal llve-cell lmaglng analysls Lo Lrack Lhe morphogeneuc process sLarung
eplLhellal cells surroundlng a hollow lumen. We reporL LhaL ln addluon Lo acuvely malnLalnlng
aplcobasal polarlLy, Lhe sLrucLures underwenL roLauonal mouons aL raLes of 13-20 m/h and Lhe
sLrucLures roLaLed 360 every 4 h durlng Lhe early phase of morphogenesls. 8oLauonal mouon was
lndependenL of Lhe cell cycle, buL was blocked by loss of Lhe eplLhellal polarlLy proLelns Scrlbble or
ard3, or by lnhlbluon of dyneln-based mlcroLubule moLors. lnLeresungly, none of Lhe sLrucLures
derlved from human cancer underwenL roLauonal mouon. We found a dlrecL relauonshlp beLween
roLauonal mouon and assembly of endogenous basemenL membrane maLrlx around Lhe 3u
sLrucLures, and LhaL sLrucLures LhaL falled Lo roLaLe were defecuve ln weavlng exogenous lamlnln
maLrlx. ulssoluuon of basemenL membrane around maLure, nonroLaung aclnl resLored roLauonal
movemenL and Lhe ablllLy Lo assemble exogenous lamlnln. 1hus, coordlnaLed roLauonal movemenL
ls a unlque mechanophyslcal process observed durlng normal 3u morphogenesls LhaL regulaLes
lamlnln maLrlx assembly and ls losL ln cancer-derlved eplLhellal cells.
Conc|us|ons
kesu|ts
Methods
8ackground
Wang eL al. nAS. 2013, 110: 163-168.
CusLomer Servlce
Link ideas in your
abstract
Conc|us|on
1hus, coordlnaLed roLauonal movemenL ls a unlque mechanophyslcal process observed durlng normal
3u morphogenesls LhaL regulaLes lamlnln maLrlx assembly and ls losL ln cancer-derlved eplLhellal cells.
8ackground
organlsms and Lhree-dlmenslonal (3u) cell culLure models whereln mammallan eplLhellal cells undergo
morphogenesls Lo form mulucellular spheres wlLh a hollow cenLral lumen. Powever, Lhe
mechanophyslcal properues assoclaLed wlLh eplLhellal morphogenesls are poorly undersLood.
Wang eL al. nAS 2013, 110:163-168.
nowever, the mechanophys|ca| propernes assoc|ated
w|th ep|the||a| morphogenes|s are poor|y undersood.
1hus, coord|nated rotanona| movement |s a un|que
mechanophys|ca| process.
rob|em
Answer
Thank you!
Any questions?
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Q%"&4)1 3+1+(<%4
Section 4
!ournal selecuon
Timing
Choose your target [ourna|:
Afet you have declded you have enough
resulLs for a publlcauon
Afet you have declded on how hlgh Lo alm-
hlgh, medlum or low lmpacL
Afet you have declded how broadly relevanL
your ndlngs are
8efote wrlung Lhe 1lLle, AbsLracL, lnLroducuon
or ulscusslon secuons
!ournal selecuon
Factors to consider when
choosing a journal
A|ms & scope keadersh|p
Cpen access
R$2($ C)(#%& 23 ,%3# 2,6%&#)4# #% -%"S
Impact factor
T)&2+3 5- J+1*
!ournal selecuon
Choosing a target journal
S|gn|hcance
A|ms and Scope
Impact
!ournal selecuon ,"3# be based on an
honesL evaluauon of -%"& ndlngs
!ournal selecuon
Evaluating significance
now new are your hnd|ngs? Nove|ty
now broad|y re|evant are your hnd|ngs? ke|evance
What are the |mportant rea|-wor|d
app||canons?
Appea|
!ournal selecuon
Insert your proposed abstract
Journal Selector
www.edanzediting.com/journal_selector
!ournal selecuon
kecommended [ourna|s
I||ter by:
Impact factor
ub||sh|ng frequency
Cpen access
Journal Selector
!ournal selecuon
Semannc match|ng terms
Iourna|s II, A|ms & Scope,
and Irequency
S|m||ar pub||shed arnc|es
nave they pub||shed s|m||ar arnc|es &+(+4#1-?
nave you (2#+* some of these arnc|es?
Journal Selector
!ournal selecuon
Visit journal websites
!ournal selecuon
Tips to identify the most
suitable journal
S
Idennfy the
|nterests of the
U%"&4)1 +*2#%&
Idennfy the
|nterests of the
&+)*+&3
Ld|tor|a|s
kev|ew arnc|es
Spec|a| |ssues
Most v|ewed
Most c|ted
?%=+& 1+V+&3
Section 5
Coverage and
SLamng lan
Cover leuers
Abstract:
I|rst |mpress|on for readers
Cover |euers are the hrst |mpress|on for
the [ourna| ed|tor
S|gn|hcance
ke|evance
Leve| of Lng||sh
Interesnng to
the|r readers?
Is your work
|mportant?
Coverage and
SLamng lan
Cover leuers
uear ur Craeber,

lease nd enclosed our manuscrlpL enuLled Amylold-llke lncluslons ln Lhe bralns of PunungLon's dlsease pauenLs", by
McCowan eL al., whlch we would llke Lo submlL for publlcauon as a 8esearch aper ln Neotoqeoeucs.

8ecenL lmmunohlsLochemlcal sLudles have revealed Lhe presence of neuronal lncluslons conLalnlng an n-Lermlnal poruon of
Lhe muLanL hunungun proLeln and ublqulun ln Lhe braln ussues of PunungLon's dlsease (Pu) pauenLs, however, Lhe role of
Lhese lncluslons ln Lhe dlsease process has remalned unclear. Cne suspecLed dlsease-causlng mechanlsm ln PunungLon's
dlsease and oLher polygluLamlne dlsorders ls Lhe poLenual for Lhe muLanL proLeln Lo undergo a conformauonal change Lo a
more sLable anu-parallel !-sheeL sLrucLure.

1o conrm lf Lhe lmmunohlsLochemlcally observed hunungun- and ublqulun-conLalnlng lncluslons dlsplay amylold feaLures, we
performed Congo red sLalnlng and boLh polarlzlng and confocal mlcroscopy on posL-morLem human braln ussues obLalned
from ve Pu pauenLs, Lwo Au pauenLs, and Lwo normal conLrols. Congo red sLalnlng revealed a small number of amylold-llke
lncluslons showlng green blrefrlngence by polarlzed mlcroscopy, ln a varleLy of corucal reglons.... ..deLecLed lncluslons
observed ln parallel secuons, suggesung LhaL only a relauvely small proporuon of lncluslons ln Pu adopL an amylold-llke
sLrucLure.

We belleve our ndlngs wlll be of parucular lnLeresL Lo Lhe readershlp of Neotoqeoeucs, whlch lncludes researchers and
cllnlclans sLudylng Lhe geneuc and molecular mechanlsms underlylng neurodegenerauve dlseases. 1herefore, we feel LhaL your
[ournal provldes Lhe mosL sulLable plauorm for Lhe dlssemlnauon of our work Lo Lhe research communlLy.

G|ve the
background to
the research
What was
done and what
was found
Interest to
[ourna|'s readers
A good cover letter
We would also llke Lo suggesL Lhe followlng revlewers for our manuscrlpL.

Ld|tor's name
Manuscr|pt nt|e
ub||canon type
kecommend rev|ewers
WB"3#X$)=+Y 3#)#+,+4#3
Coverage and
SLamng lan
Cover leuers
"Must-have"
statements
Not subm|ued to
other [ourna|s
Source of
fund|ng
Authors agree on
paper][ourna|
Cr|g|na| and
unpub||shed
No con|cts of
|nterest
Authorsh|p
contr|bunons
Disclaimers about
publication ethics
Coverage and
SLamng lan
Cover leuers
Recommending
reviewers
When submlmng your paper, you musL provlde Lhe
names, amllauons, and valld e-mall addresses of ve (3)
revlewers. lf you do noL do so, your paper wlll be
reLurned, unrevlewed."
AuLhors are requesLed Lo provlde Lhe names and full
addresses (lncludlng e-mall address) of up Lo four poLenual
referees."
Coverage and
SLamng lan
Cover leuers
Recommending
reviewers
Where to hnd
them?
Irom your read|ng]references,
network|ng at conferences
now sen|or? A|m for m|d-|eve| researchers
Who to avo|d?
Co||aborators (past S years),
researchers from same |nsntunon
I4#+&4)<%4)1 123#.
1 or 2 from As|a, 1 or 2 from Lurope, and 1 or 2 from North Amer|ca
>++& D+=2+8
Section 6
eer revlew
What reviewers
are looking for
1he sc|ence
1he manuscr|pt
! ke|evant hypothes|s
! Good exper|menta| des|gn
! Appropr|ate methodo|ogy
! Good data ana|ys|s
! Va||d conc|us|ons
! Log|ca| ow of |nformanon
! Manuscr|pt structure and formamng
! Appropr|ate references
! n|gh readab|||ty
!53#&)(# )4* I4#&%*"(<%4
B+#$%*3 D+3"1#3 )4* Z20"&+3 L23("332%4
eer revlew
kesponse
|euer
kespond to +=+&- rev|ewer comment
:)3- to see
changes
kefer to ||ne and page numbers
Use a d|erent co|or font
n|gh||ght the text
Revision
eer revlew
Writing a response letter
Marc Llppman, Mu
LdlLor-ln-Chlef
8teost coocet keseotcb ooJ 1teotmeot

3 SepLember 2013

uear ur Llppman,

8e: 8esubmlsslon of manuscrlpL reference no. W!S-07-3739

lease nd auached a revlsed verslon of our manuscrlpL orlglnally enuLled Lvaluauon of Lhe Clasgow prognosuc
score ln pauenLs undergolng curauve resecuon for breasL cancer llver meLasLases," whlch we would llke Lo
resubmlL for conslderauon for publlcauon ln Lhe 8teost coocet keseotcb ooJ 1teotmeot.

1he revlewer's commenLs were hlghly lnslghuul and enabled us Lo greaLly lmprove Lhe quallLy of our manuscrlpL. ln
Lhe followlng pages are our polnL-by-polnL responses Lo each of Lhe commenLs.

8evlslons ln Lhe manuscrlpL are shown as underllned LexL. ln accordance wlLh Lhe rsL commenL, Lhe uLle has been
revlsed and Lhe enure manuscrlpL has undergone subsLanual Lngllsh edlung.
We hope LhaL Lhe revlslons ln Lhe manuscrlpL and our accompanylng responses wlll be sumclenL Lo make our
manuscrlpL sulLable for publlcauon ln Lhe 8teost coocet keseotcb ooJ 1teotmeot.
Address ed|tor persona||y
Manuscr|pt ID number
1hank rev|ewers
n|gh||ght ma[or changes
eer revlew
Agreeing with reviewers
D+=2+8+& ?%,,+4#. lo yoot ooolysls of tbe Joto yoo bove cboseo
to ose o somewbot obscote fmoq foocuoo (teqtessloo). lo my
oploloo, o slmple Coossloo foocuoo woolJ bove so[ceJ. Moteovet,
tbe tesolts woolJ be mote losttocuve ooJ eoslet to compote to
ptevloos tesolts.
kesponse: We agree wlLh Lhe revlewer's assessmenL of Lhe
analysls. Cur Lallored funcuon, ln lLs currenL form, makes lL dlmculL
Lo Lell LhaL Lhls measuremenL consuLuLes a slgnlcanL
lmprovemenL over prevlously reporLed values. We descrlbe our
new analysls uslng a Causslan mng funcuon ln our revlsed 8esulLs
secuon (age 6, Llnes 12-18).
eer revlew
oploloo, o slmple Coossloo foocuoo woolJ bove so[ceJ. Moteovet,
tbe tesolts woolJ be mote losttocuve ooJ eoslet to compote to
ptevloos tesolts.
kesponse: We agree wlLh Lhe revlewer's assessmenL of Lhe
analysls. Cur Lallored funcuon, ln lLs currenL form, makes lL dlmculL
Lo Lell LhaL Lhls measuremenL consuLuLes a slgnlcanL
lmprovemenL over prevlously reporLed values. We descrlbe our
new analysls uslng a Causslan mng funcuon ln our revlsed 8esulLs
secuon (age 6, Llnes 12-18).
Agreeing with reviewers
Agreement
kev|s|ons
Locanon
eer revlew
Disagreeing with reviewers
oploloo, o slmple Coossloo foocuoo woolJ bove so[ceJ.
Moteovet, tbe tesolts woolJ be mote losttocuve ooJ eoslet to
compote to ptevloos tesolts.
kesponse: AlLhough a slmple Causslan L would faclllLaLe
comparlson wlLh Lhe resulLs of oLher sLudles, our Lallored funcuon
allows for Lhe analysls of Lhe daLa ln Lerms of Lhe SmlLh model
SmlLh eL al., 1998. We have now explalned Lhe use of Lhls
funcuon and Lhe SmlLh model ln our revlsed ulscusslon secuon
(age 12, Llnes 2-6).
eer revlew
oploloo, o slmple Coossloo foocuoo woolJ bove so[ceJ.
Moteovet, tbe tesolts woolJ be mote losttocuve ooJ eoslet to
compote to ptevloos tesolts.
kesponse: AlLhough a slmple Causslan L would faclllLaLe
comparlson wlLh Lhe resulLs of oLher sLudles, our Lallored funcuon
allows for Lhe analysls of Lhe daLa ln Lerms of Lhe SmlLh model
SmlLh eL al., 1998. We have now explalned Lhe use of Lhls
funcuon and Lhe SmlLh model ln our revlsed ulscusslon secuon
(age 12, Llnes 2-6).
kev|s|ons
Locanon
Lv|dence
Disagreeing with reviewers
eer revlew
Unfair reviewer
comments
D+=2+8+& (%,,+4#. cotteotly, tbe ootbots cooclosloo tbot tbls
qeoe ls lovolveJ lo beott Jevelopmeot ls oot completely vollJoteJ
by tbelt lo vltto ooolyses. 1bey sboolJ Jo oJJluoool lo vlvo
expetlmeots osloq o qeoeuc moose moJel to sbow tbot beott
Jevelopmeot ls teqoloteJ by tbls qeoe.
D+)3%43 8$- &+=2+8+&3 ,20$# ,)N+ #$+3+ (%,,+4#3
" Current resu|ts are not appropr|ate for the |mpact factor of
the [ourna|
" kev|ewer |s be|ng "unfa|r"
eer revlew
Unfair reviewer
comments
R$)# -%" 3$%"1* *%
" Do the exper|ments, rev|se, and resubm|t to the same
[ourna|
" W|thdraw subm|ss|on and resubm|t current
manuscr|pt to a |ower |mpact factor [ourna|
" Contact the [ourna| ed|tor |f you fee| rev|ewer |s be|ng
unfa|r
eer revlew
If rejected, what
should you do?
Cpnon 1: New subm|ss|on to the 3),+ [ourna|
" Iu||y rev|se manuscr|pt
" repare po|nt-by-po|nt responses
" Inc|ude the or|g|na| manuscr|pt ID number
Cpnon 2: New subm|ss|on to a *2;+&+4# [ourna|
" kev|se manuscr|pt
" keformat accord|ng to the author gu|de||nes
If accepted, whats next?
" romote your work on soc|a| networks
1wluer, Llnkedln, 8esearch CaLe
" kespond to post-pub||canon comments
" resent your work at conferences
romoLe your publlcauon
Allows you Lo dlscuss your work personally wlLh your peers
CeL feedback abouL your work and fuLure dlrecuons
Be an effective communicator
S
/%"& 0%)1 23 4%# %41- #% 5+ 6"5123$+*7
5"# )13% #% 5+ 82*+1- &+)*9(2#+*
! Wr|te eecnve|y
! Log|ca||y present your research |n your manuscr|pt
! Choose the best [ourna| to reach your target aud|ence
! Convey the s|gn|hcance of your work to [ourna| ed|tors
! roper|y rev|se your manuscr|pt aher peer rev|ew
! romote your research aher pub||canon
Thank you!
Any questions?
Io||ow us on 1w|uer
QIourna|Adv|sor
L|ke us on Iacebook
facebook.com]Iourna|Adv|sor
Down|oad and further read|ng
edanzed|nng.com]Ind|a_2014
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www.edanzed|nng.com

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