Anaerobic Cellular Respiration SB14U - Biology

Jim Kasprzak

Agenda
Curriculum Smarter Science Competition Lactate Fermentation Clothespin Marathon Ethanol Fermentation Various Labs/Activities

Curriculum Expectations
Overall Expectations c2. investigate the products of metabolic processes such as cellular respiration and photosynthesis; c3. demonstrate an understanding of the chemical changes and energy conversions that occur in metabolic processes

Curriculum Expectations
Specific Expectations C2.2: conduct a laboratory investigation into the process of cellular respiration to identify the products of the process, interpret the qualitative observations, and display them in an appropriate format

c3.1 explain the chemical changes and energy conversions associated with the processes of aerobic and anaerobic cellular respiration
c3.4 describe, compare, and illustrate (e.g., using flow charts) the matter and energy transformations that occur during the processes of cellular respiration (aerobic and anaerobic) and photosynthesis, including the roles of oxygen and organelles such as mitochondria and chloroplasts

Lactic Acid Fermentation

Lactate (Lactic Acid) Fermentation

Normal conditions (optimum oxygen levels) humans/mammals use aerobic respiration During strenuous activity (shortage of oxygen)
Muscle cells respire glucose faster than oxygen can be supplied Lactate (Lactic Acid) Fermentation begins

Lactate (Lactic Acid) Fermentation

2 NADH produced in step 6 of glycolysis

Lactate (Lactic Acid) Fermentation

2 NADH produced in step 6 of glycolysis 2 Pyruvate is the result of glycolysis

Lactate (Lactic Acid) Fermentation

2 NADH produced in step 6 of glycolysis 2 Pyruvate is the result of glycolysis Pyruvate reduced to lactate NADH oxidizes to NAD+

NAD+ is free to return to glycolysis

Lactate (Lactic Acid) Fermentation

Lactate accumulation Muscle stiffness, soreness, fatigue Lactate transferred through blood stream to the liver

Exercise stops: Aerobic respiration = efficient Lactate is oxidized back to pyruvate Continue through the Krebs cycle Produce more ATP than possible anaerobically

Oxygen Debt Competition

Radiolab

STSE
Relate Lactic Acid to athletes in the class
Better training = more efficient system

Example: Julie Moss Make yogurt Labs

Julie Moss

Activity Questions

Further Investigations
Design an experiment to help you answer one of the following questions: Is there a difference between the writing hand and the non-writing hand? How long a period of rest do your finger muscles need before you can repeat the experiment and get the same results as your first trial? Is there a gender difference in finger muscle fatigue? Do the fittest students in the class also have the fittest fingers? Do the musicians in the class have the fittest fingers?

What happened to your strength as you progressed through each trial? Describe how your hand and fingers felt during the end of your trials. What factors might cause you to get more squeezes (to have less fatigue)? Were your results different for the dominant and the nondominant hand? Explain why they would be different. Your muscles would probably recover after 10 min of rest to operate at the original squeeze rate. Explain why.

Ethanol Fermentation

Ethanol Fermentation

Ethanol Fermentation
CO2 removed from pyruvate Enzyme: pyruvate decarboxylase Forms 2 acetaldehyde

Ethanol Fermentation
CO2 removed from pyruvate Enzyme: pyruvate decarboxylase Forms 2 acetaldehyde Acetaldehyde reduced to ethanol NADH oxidizes to NAD+ NAD+ can be used in glycolysis again

CO2 & Ethanol removed as waste

Labs/Activities
Links can be found on the website:
Smarter Science Posters Who can hold their breath the longest Clothes Pin Marathon Making Vinegar Many Variations of the Yeast Lab

Pictionary Countdown