4F3 anti-discs large INVESTIGATOR Name Corey Goodman Address University of California, Berkeley, Department of Molecular and Cell

Biology, 519 Life Sciences Addition, Berkeley, CA 94720 Phone (510) 643-3465 IMMUNOGEN Substance Name Origin Chemical Composition Developmental Stage IMMUNIZATION PROTOCOL Donor Animal Species Strain Sex Organ and tissue Immunization Dates immunized Amount of antigen Route of immunization Adjuvant FUSION Date Myeloma cell line Species Designation MONOCLONAL ANTIBODY Isotype Specificity Cell binding Immunohistology Antibody competition Species Specificity

Discs-Large PDZ2 domain Dan Woods/Peter Bryant from Drosophila GST-PDZ2 in pGEX purified on glutathion A beads Dlg is found at all developmental stages

mouse Balb/c female spleen 50 g/mouse/injection, 100 g final boost IP RIBI

mouse P3 x 63 Ag8.653

IgG1, kappa light chain

works in Drosophila, not tested in other species

ANTIGEN Chemical properties Molecular weight Characterization Immunoprecipitation yes Immunoblotting yes Purification Amino acid sequence analysis Functional effects yes Immunohistochemistry PUBLICATIONS : Parnas, D., Haghighi, A.P., Fetter, R.D., Kim, S.W., and Goodman, C.S. (2001). Regulation of postsynaptic structure and protein localization by the Rho-type guanine nucleotide exchange factor dPix. Neuron 32(3), 415-424. Masuda-Nakagawa, L.M., Tanaka, N.K., and OKane, C.J. (2005). Stereotypic and random patterns of connectivity in the larval mushroom body calyx of Drosophila. PNAS 102(52), 19027-19032. Wang, X., Shaw, W.R., Tsang, H.T.H., Reid, E., and OKane, C.J. (2007). Drosophila spichthyin inhibits BMP signaling and regulates synaptic growth and axonal microtubules. Nat. Neurosci. 10(2), 177-185. Rotte, C., Walz, B., and Baumann, O. (2008). Morphological and functional characterization of the thoracic portion of blowfly salivary glands. Arthropod. Struct. Dev. 37, 372-382.

ACKNOWLEDGMENTS STATEMENT We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of the DSHB's products. We suggest that the following statement be used: "The (hybridoma or monoclonal antibody) developed by [Investigator(s)] was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biology, Iowa City, IA 52242. Please send copies of all publications resulting from the use of Bank products to: Developmental Studies Hybridoma Bank Department of Biology The University of Iowa 028 Biology Building East Iowa City, IA 52242