JERAD Vol. 8 No.

03, January-March 2014

J. Environ. Res. Develop.

From The Editors Desk

Reactive Oxygen Species (ROS) generated by oxidative stress caused due to various environmental factors and climatic conditions has been associated with human ailment and food deteriorations. Free radical mediated oxidative damage to biomolecules including protein and DNA has been complicated with cancer disease development. Role of antioxidants in combating oxidative stress related problem has been well established and search for novel anti-oxidant agents is one of the thrust areas of research in biomedical sciences, cosmetic and food industries. The current researches describe isolation and characterization of a natural potential antioxidant from Aegle marmelos leave. Boiling water extracts are usually being prepared from A. marmelos fresh leaves and extracts are further fractionated with liquidliquid partitioning method into n-hexane, diethyl ether, ethyl acetate, methanol, ethanol and water fractions. The GC-MS and LC-MS study indicated the presence of gallic acid, querecetin and ferulic acid as major antioxidant constituents. The results obtained from DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging activity assay revealed that only ethyl acetate and methanol fraction showed most of the scavenging activities with EC50 concentration 20 and 14 g per ml extract fraction, respectively. The deoxyribose degradation experiments are carried out using hydroxyl radical generated from iron mediated Fenton reaction. The results revealed significant hydroxyl radical scavenging potential with EC50 108 and 86 g per ml extract fraction for ethyl acetate and methanol fraction, respectively. The ethyl acetate and methanol fractions didnt show pro-oxidant activities in the absence of reducing

JERAD Vol. 8 No. 03, January-March 2014

J. Environ. Res. Develop.

agent. The protective potential against oxidative damage to calf thymus DNA and plasmid are studied on 1% agarose gel using copper (II) and ascorbate reaction mixture as source of free radical damage. The densitometric analysis of DNA band intensity are performed to study the extent of protection against control DNA damage. The results showed marked inhibition potential against oxidative damage to DNA with EC50 120 and 93 g per ml extract fraction for ethyl acetate and methanol fraction, respectively. The protective effect of ethyl acetate and methanol fraction on hypochlorite mediated oxidative damage to bovine serum protein are studied based on native PAGE and spectrophotometer technique. The results revealed that both fractions ethyl acetate and methanol showed significant inhibition potential with EC50 220 and 158 g per ml extract fraction, respectively. On the basis of above findings, scientists found these two fractions ethyl acetate and methanol as promising source of antioxidant activities and convincing protective role against oxidative damage to DNA and protein. Subhash C. Pandey

JERAD Vol. 8 No. 03, January-March 2014

J. Environ. Res. Develop.

JOURNAL OF ENVIRONMENTAL RESEARCH AND DEVELOPMENT

(An official publication of Natural Sciences, Technology, Social Sciences, Law and Management for Environment)

J. Environ. Res. Develop. URL : www.jerad.org ISSN 0973 6921 E-ISSN 2319-5983

An open access peer reviewed online and print research journal

Volume : 08 Number : 03 January-March 2014

CONTENTS
NATURAL SCIENCE Full Length Papers :
1. Development of liquid formulation for the dual purpose of crop protection and production Agrawal Pushpa, Pandey Subhash C. and Manjunatha Reddy A.H. 2. Assessment of ambient air quality index of Surat city during early morning hours Miyajan S. Juned and Desai Hemangi 3. Bioremediation of some Polycyclic Aromatic Hydrocarbons (PAH) from soil using Sphingobium indicum, Sphingobium japonicum and Stenotrophomonas maltophilia bacterial strains under aerobic conditions Gurjeet, Priyanka, N. C. Kothiyal and Vaneet Kumar 4. An annual study of stomatocytes : A red cell anomaly in the male and female false vampire bat Megaderma lyra lyra (Geoffroy) Sastry M.S. and Gaurkar Rekha 5. Isoaltion, characterization and identification of rhizospheric bacteria with the potential for biological control of Sida acuta Vishwas Shankar Patil 6. Laminated composite of filler loaded paper sheets manufactured using rice straw fibers and urea formaldehyde resin Sinha Sanjay Kumar Akhouri and Samarjeet Singh 7. Heavy metal analysis in water, fish and vegetative samples of Lakha-Banjara lake, Sagar, Madhya Pradesh, India Y. Rohan, Khan Imran and Pandit J. Umar 8. Identification of a newly isolated p-nitrophenol characterized as Rhodococcus sp. BUPNP1 Sengupta Kriti and Saha Pradipta degrading strain 378-383

384-394

395-405

406-410

411-417

418-424

425-430

431-436

9. Synthesis, characterization and antimicrobial activity studies of (E)-N-(2-(2(Substituted Benzaldehyde) Hydrazinyl) Quinazoline-4-YL) Isonicotinohydrazide Naik P.J. and Desai P.S.

437-442

JERAD Vol. 8 No. 03, January-March 2014

J. Environ. Res. Develop.

10. Applications of water quality index for ground water quality assessment on Tamil Nadu and Pondicherry, India Sirajudeen J. and Abdul Vahith R. 11. Characterization of culturable bacterial communities in petroleum hydrocarbon contaminated sludge of oil refineries and oil exploration sites Ajoy Roy, Siddhartha Pal, Sufia K. Kazy, Poulomi Sarkar, Pinaki Sar and Aloke K. Ghoshal

443-450

451-458

Review Paper :
12. Qualitative and quantitative evaluation of water sources of few areas in and around Gwalior, Madhya Pradesh, India Saxena Richa and Sharma Manju 459-469

TECHNOLOGY Full Length Papers :

13. The seepage through the Earth dams with a vertical drain : An experimental study DJEHICHE Abdelkader, AMIEUR Rekia and GAFSI Mostafa 14. Direct write manufacturing of solid oxide fuel cells for green energy Salil Desai, Man Yang, Zhigang Xu and Jag Sankar 15. Multi-spectral satellites and GIS modeling of hydrological characteristics of Tighra reservoir, Gwalior, India Bidyalakshmi Phurailatpam, Rao R.J., Kush Kushwah and S.N. Prasad 16. Modeling hydrological response to land use change in watersheds of Viti Levu Island, Fiji Dadhich P. Ankita and Nadaoka Kazuo 17. Performance evaluation of polymer modified bitumen in flexible pavement Tapase A.B. and Kadam D.B. 471-476

477-483 484-491

492-503

504-509

Review Paper :
18. Role of compression in treatment of leg ulcers Monica Sikka, S. Ghosh and A. Mukhopadhyay 510-513

SOCIAL SCIENCE Full Length Papers :

19. Assessing river basin system potentials to enhance sustainable irrigation farming operations and management in Nigeria Oriola E. O. and Alabi M.O. 20. Impact of a gas based power plant on the air quality of Delhi, India Saurabh Kumar and P.Goyal 21. Predicting efficiency of treatment plant by multi parameter aggregated index Khambete Anjali K. and Christion R.A. 515-522

523-529 530-539

JERAD Vol. 8 No. 03, January-March 2014

J. Environ. Res. Develop.

22. Long-term impact of transhumant pastoralism on forest cover : A case study from Northern Liddar valley of Kashmir Himalaya Tasadoq H. Jaweed , Saptarshi G. Praveen, Kodre Swapnil and Gaikwad W. Sunil 23. A study on forest loss due to river migration in Sonai-Rupai wildlife sanctuary of Assam, India using geoinformatics Saherah Jahan, Sarbeswar Kalita and Binode Kumar Baruah

540-551

552-560

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