Memorias Congreso Completas

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ORGANIZADOR GENERAL

Dra. Patricia Gorocica Rosete
Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas
gorocicap@gmail.com
Tel 54871705

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31 DE JULIOA L 2 DE AGOSTO 2013

PALACIO DE MEDICINA

CIUDAD DE MEXICO

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CONSEJO DIRECTIVO DE LA SOCIEDAD
LATINOAMERICANA DE GLICOBIOLOGIA

Dr. Ivn Martnez Duncker R PRESIDENTE
Facultad de Ciencias, Universidad Autnoma del Estado de Morelos, Mxico
Dra. Blanca Ortiz Quintero, SECRETARIO
Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas
,Mxico
Dra. Patricia Gorocica Rosete, TESORERO
Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas,
Mxico
Dr. Hctor Mora Montes, VOCAL
Departamento de Biologa, Universidad de Guanajuato, Mxico
Dr. Julio Reyes Leyva , VOCAL
Centro de Investigacin Biomdica de Oriente (CIBIOR) , Puebla, Mxico
Dr. Jos Luis Montiel Hernndez, VOCAL
Facultad de Farmacia, Universidad Autnoma del Estado de Morelos,
Mxico
Dr. Miguel ngel Mayoral Chvez VOCAL
Facultad de Medicina, Universidad Benito Jurez de Oaxaca, Mxico

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PROGRAMA DE INAGURACION

PRESENTACIN DE LA SOCIEDAD LATINOAMERICANA DE
GLICOBIOLOGA
Dr. Ivn Martnez Drunker
Presidente de la Sociedad

Mesa de Honor

Dr. Juan Jos Hicks Gmez
General de Polticas de Investigacin en Salud en la CCINSHAE
Dr. Jorge Salas Hernndez
Director del Instituto Nacional de Enfermedades Respiratorias Ismael Cosio
Villegas
Dr. Enrique Graue Weichers
Director de Facultad de Medicina de la UNAM.
Dr. Gabriel Cuevas Gonzlez Bravo.
Director del Instituto de Qumica de la UNAM
Dr. Octavio Tonahiu Ramrez Reivich
Director del Instituto de Biotecnologa de la UNAM
Dra. Patricia Gorocica Rosete
Coordinador general del Congreso
Dr. Edgar Zenteno Galindo
Miembro Honorario de la Sociedad

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PATRCINADORES

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PROGRAMA DETALLADO DEL CONGRESO
MIERCOLES 31 DE JULIO
Curso precongreso
TEMA EXPOSITOR
"Estructura de glicanos, nomeclatura y papel biolgico"
Dra. Blanca ESPINOSA,
INER
"Biosntesis de glicanos y las consecuencias por el deficit en su
biosntesis"
Dr. Miguel Angel
MAYORAL, UABJO
"Funcin de los glicanos en la invasin de microrganismos "
Dr. Hctor MORA . U DE
GTO.
"Papel de los glicanos en la respuesta inmune"
Dr. Ricardo LASCURAIN.
UNAM
"Estrategas generales para abordar el estudio de glicanos
Dr. Antonio Serrato.
INER
Herramientas qumicas en la Glicobiologa.
Dra. Yobana PREZ.
UABJO
"Lectinas como herramientas para el estudio de la Glicobiologia"
Dr. Pedro Hernandez
Cruz. UABJO
"Determinacin de Carbohidratos por Cromatografa Inica mediante
Deteccin Amperomtrica
Q.I Marco Antonio
BUSTAMENTE ACEVEDO.
METROHM
CEREMONIA INAGURAL
SESION GENERAL 1 CONFERENCIAS INAGURALES

AUDITORIO DR.
GUSTAVO BAZ PRADA
Coordinador: Dr. Ivn Martnez Druncker
"Biological Roles of Glycans: a Look Back Over the Decades"
Dr. Ajit VARKI.
University of California,
San Diego, EUA
Tools for identifying new human glycosylation disorders
Dr. Hudson FREEZE.
Sanford Burnham
Institute, EUA.

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COCKTAIL DE BIENVENIDA PATIO CENTRAL
JUEVES 1 DE AGOSTO
SYMPOSIUM "TECNICAS PARA EL ESTUDIO DE LA GLICOBIOLOGIA"

AUDITORIO DR. GUSTAVO
BAZ PRADA
Coordinador: Dr. Edgar Zenteno
"Bioingenieria aplicada (Nucleasas con dedos de Zinc y modificacione
de genomas en sistemas eucarioticos)."
MsC David Pierre Pillon.
Sigma Aldrich de Mxico
Uso de la espectrometra de masas para la identificacin de residuos
de carbohidratos
Dra. Victoria PANDO.
Instituto Nacional de
Salud Publica , Mxico
Metabolic Glycoengineering; Implications in the Intake of Azide Non-
Natural Sugar Analogs
Dr. Rubn ALMARAZ.
University of Michigan ,
EUA
La interaccin CH/H, base del reconocimiento protena carbohidrato y
su aplicacin a la sntesis de catalizadores.
Dr. Gabriel CUEVAS
Instituto de Qumica de
la UNAM.
La citometra de flujo como herramienta para el estudio de la
glicobiologa
Dra. Lourdes A.
ARRIAGA Pizano. Centro
Mdico Nacional Siglo
XXI,Mxico
Glicosilacin de protenas en sistemas de cultivo celular.
Dr. Antonio SERRATO .
Departamento de
Bioqumica, INER
COFFE BREAK
SESION GENERAL 2 GLICOBIOLOGIA MEDICA

Coordinador: Dr. Hector Mora
BAZ PRADA
Defectos gneticos de glicosilacin
Dr. Ivn MARTNEZ
DUNCKER. Fac,
Ciencias,UAEM, Mxico
Glycobiology in skeletal dysplasias associated to Congenital Disorder of
Glycosylation"
Dra. Carla ASSTEGIANO.
Universidad Nacional de
Crdoba,Argentina.
COMIDA

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SESION GENERAL 3 GLICOBIOLOGIA DE LA ENFERMEDADES INFECCIOSAS

BAZ PRADA
Coordinador: Dr. Jos Luis Montiel
"El zimozn como potente inductor de la respuesta inmunitaria en el
mosquito Anopheles albimanus"
Dr. Humberto LANZ.
Salud Publica-CISEI
Glicobiologa de la pared celular de Candida albicans
Dr. Hctor MORA
MONTES. Universidad
de Guanajuato, Mxico
"Fungal cell wall glycococonjugates as virulence factors: true or false?"
Dra. Leila M. LOPES-
BEZERRA. Universidade
do Estado do Rio de
Janeiro, Brasil

Glicobiologa de hongos y protozoarios patgenos de humanos: un
recuento de historias no terminadas"
Dr. Everardo LPEZ
ROMERO. Universidad
de Guanajuato, Mxico.
COFFE BREAK
SESION DE CARTELES PATIO CENTRAL
Coordinador: Dr. Pedro Hernndez
SESION DE TRABAJOS LIBRES
AUDITORIO
PARANINFO
Coordinador. Dr. Ricardo Lascurain
VIERNES 2 DE AGOSTO
SESION GENERAL 4 GLICOBIOTECNOLOGIA

BAZ PRADA
Coordinador. Dr. Everardo Lpez Romero
Papel de la N-glicosilacin en la estabilidad e inmunogenicidad de una
vacuna recombinante contra influenza.
Dra. Laura A.
PALOMARES. IBT,
UNAM, Mxico
Protein glycosylation systems in bacterial pathogens and their
promising applications in glycoengineering
Dr. Mario FELDMAN.
University of Alberta,
Canada.
Development of Streptococcus pneumoniae conjugate vaccine with
the most prevalent serotypes in Latin America.
Dr. Vicente VEREZ
BENCOME. Centro de
Qumica Biomolecular,
La Habana, Cuba.

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"Carbohydrate engineering in Escherichia coli K-12.
Dr. Bernard
PRIEM.CNRS-CERMAV,
Grenoble Francia
COFFE BREAK
SESION GENERAL 5 BIOSINTESIS Y METABOLISMO DE GLICOCONJUGADOS

BAZ PRADA
"Glycobiologie des voies de signalisation et pathologies associes-
Fonctions associes la O-GlcNAcylation"
Dr. Tony LEFEBVRE.
Unit de Glycobiologie
Structurale et
Fonctionnelle, Lille
France
Nueva Regulacion de Reacciones Posttraduccional en el Aparato de
Golgi: desde Ciencia Basica a Patologias
Dr. Carlos HIRSCHBERG.
Boston University, USA .
UDP-glucose transporters and their role in protein quality control in
the endoplasmic reticulum
Dr. Ariel ORELLANA.
Universidad Andrs
Bello, en Santiago, Chile
"O-GlcNAcylation, diabetes and cancer
Dr. Tarik ISSAD. INSERM,
Francia.
COMIDA
SESION GENERAL 6 MODULACION DE LA RESPUESTA INMUNE Y
GLICOBIOLOGIA DEL CANCER
Coordinador: Dr. Miguel Mayoral

"Galectins in host-pathogen interactions and cancer: Structural and
functional aspects
Dr. Gerardo VASTA .
University of
Maryland,Baltimore,
EUA.
Role for Galectin-Glycan interactions in tuning the immune responses
Dra. Martha TOSCANO.
Inst. Biol. y Med. Exp,
Argentina.
"O-GlcNAcylation : a novel regulator of cell signaling and cell cycle
Dra. Vannesa
DEHENAUT . Institut de
Biologie de Lille,
Universit de Lille Nord
de France
New clinical and preclinical observations on human milk
oligosaccharides"
Dr. Pedro PRIETO. Abbot
Nutrition, EUA,
Columbia.

10
" Regulacin de la respuesta inmune innata por el Siglec-9, mediante el
empleo de un nuevo SAMP (self-associated molecular pattern): el cido
hialurnico"
Dr. Ismael SECUNDINO.
University of California,
San Diego, EUA
CLAUSURA Y PREMIACION DE TRABAJOS

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CONFRERENCISTAS EXTRANJEROS

DR: AJIT VARKI

CORREO ELECTRONICO:
a1varki@ucsd.edu

POSICION ACTUAL:
Profesor de medicina celular y
molecular, y co-director del Centro de
Investigacin y Enseanza en
Glicobiologa , University of California,
San Diego, EUA

LINEA DE INVESTIGACION:
El papel del cido silico en las
enfermedades y en las funciones
biolgicas

FORMACIN PROFESIONAL:
Formacin bsica en fisiologa,
medicina y bioqumica en la
Universidad de Nebraska y Universidad
de Washington en St. Louis.
Certificacin en medicina interna,
hematologa y oncologa.

DATOS RELEVANTES:
Presidente de la American Society of
Glicobiology. Editor del J. Clinical
Investigation, Premio emrito de NIH
,Premio de Investigacin de la Sociedad
Americana de Cncer , Premio Karl
Meyer de la Sociedad de Glycobiologa y
la Organizacin Internacional
Glicoconjugados 2007

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DR. GERARDO VASTA

CORREO ELECTRONICO:
GVasta@som.umaryland.edu

POSICION ACTUAL:
Profesor del Departamento de
Microbiologa e Inmunologa de la
Escuela de Medicina, Programa de
Biologa de Sistemas de Imagen,
Universidad de Merylan, EUA

Aspectos bioqumicos y moleculares de
las galactinas en la respuesta inmune

Licenciatura y doctorado en Bioqumica
en la Escuela de Ciencias Exactas ,
Universidad Nacional , La plata , Buenos
Aires Argentina, posdoctorado en el
departamento de medicina e
inmunologa clnica en el Roswell Park
Memorial Institute, Buffalo, NY
DATOS RELEVANTES:
Fue profesor en el Centro de
Biotecnologa Marina de la Universidad
de Maryland Biotechnology Institute
(UMBI) y asesor de Investigacin en el
Departamento de Ciencias Biolgicas de
la Universidad George Washington

CORREO ELECTRONICO:
hudson@sanfordburnham.org

POSICION ACTUAL:
Director del Programa de
Enfermedades Genticas del Centro
Infantil de Sanford, Sanford Burnham
Institute, EUA, Presidente de la
Sociedad de Glycobiologa.


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DR. HUDSON FREEZE

Enfermedades congnitas por dficit en
glicosilacin

Doctorado en la Universidad de San
Diego California en Biologa, medicina y
Neurociencias

DATOS RELEVANTES:
Se dedica a descubrir las causas
moleculares de las enfermedades
congnitas por dficit de glicosilacin
as como en cncer, diabetes,
enfermedades infecciosas,
neurodegenerativas, inflamatorias e
infecciosas en la niez.

DR. PEDRO PRIETO

CORREO ELECTRONICO:
pedro.prieto@abbott.com

POSICION ACTUAL:
Director de Investigacin ABBOT
laboratorios, Columbia, USA

Composicin de carbohidratos
complejos en leche materna

Ingeniero Bioqumico por el
Tecnolgico de Monterrey, Maestra en
Administracin por la Universidad de
Alabama, Maestra en Bioqumica y
Nutricin y el Doctorado en Bioqumica
y Nutricin, ambos por Virginia Tech,
miembro de la Facultad de Bioqumica
de la Universidad de Georgia.

DATOS RELEVANTES:
Miembro de la Sociedad honorfica de

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investigacin cientfica Sigma XI y la
Sociedad Internacional de Glicobiologa,
miembro del Consejo Asesor de la
Fundacin Zambrano Helin del
Tecnolgico de Monterrey y es titular de
Ctedra en la Universidad Autnoma de
Quertaro en Mxico.

DR. ISMAEL SECUNDINO

CORREO ELECTRONICO:
isecundinovelazque@ucsd.edu

POSICION ACTUAL:
Postdoctorado en el Laboratorio. A.
Varkin, University of California

El papel de los cidos silicos
expresadas en la cpsula de Escherichia
K1 y sus interacciones con los
receptores Siglec en macrfagos y
clulas microgliales en la patogenia de
la meningitis neonatal.

Doctorado en Inmunologa en la UNAM
Mxico. Investigacin postdoctoral en
el Instituto de Biotecnologa,
Cuernavaca, Mxico).

CORREO ELECTRONICO:
rtalmaraz@ucdavis.edu

POSICION ACTUAL:
Posdoctorado de la University of
Michigan

Aplicacin de tcnicas para el anlisis
de glicanos de clulas de cncer
pancretico. Aplicacin de tcnicas de
glicoingeniera metablicos para

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DR. RUBN ALAMARAZ
modificar las estructuras de glicanos in
vitro

Postdoctorado en la Universidad de
Michigan, ttulo de su investigacin:
"Anlisis de la glicobiologa de las
clulas del cncer de pncreas por
Espectrometra de Masas".
Posdoctorado en Johns Hopkins
University 2009-2012. Ttulo de
investigacin: "Propiedades
Glicobiolgicas y Biofsicas de la
metstasis del cncer pancretico".

DATOS RELEVANTES:
Posdoctorado de la Jonhs Hopkins
University

DR. CARLOS HIRSCHBERG
CORREO ELECTRONICO:
chirschb@bu.edu
POSICION ACTUAL:
Profesor del Departamento de Biologa
Molecular y Celular, Boston University

Biognesis, estructura y funcin de la
superficie de las clulas eucariotas
inferiores y superiores y de la matriz
extracelular, (glicoprotenas,
glicolpidos, y glicosaminoglicanos).

Doctorado obtenido en la Universidad
de Illinois, Urbana, 1970 Universidad de
Harvard, 1970-1972 Instituto
Tecnolgico de Massachusetts, 1972-
1974

DATOS RELEVANTES:
"MERIT Award NIH grant GM 30365
""Glycosylation of Cell Lipids and
Proteins"" (19912001). Pertenece a

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multiples Sociedades Cientficas
Americanas e Internacionales como
American Association for the
Advancement of Science, American
Society for Biochemistry and Molecular
Biology, American Society for Cell
Biology.

DR. MARIO FELDMAN
CORREO ELECTRONICO:
mfeldman@ualberta.ca

POSICION ACTUAL:
Profesor en el departamento de
Ciencias Biolgicas de la Universidad de
Alberta e Investigador Titular en
Alberta Glycomics Centre, Canad

Glicoingeniera de vacunas contra
microorganismos patgenos


Doctorado en la Universidad de Buenos
Aires

DATOS RELEVANTES:

Autor de mltiples artculos sobre
mecanismos de glicosilacin de la
protena en bacterias , glicoingeniera
para el diseo de vacunas y factores de
virulencia de microorganismos

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DRA. MATHA TOSCANO

CORREO ELECTRONICO:
martalitos@gmail.com

POSICION ACTUAL:
Investigador Adjunto - CONICET.
Instituto de Biologa y Medicina
Experimental (IByME). Consejo
Nacional de Investigaciones Cientficas
y Tcnicas (CONICET).

Interacciones entre galectinas y
glicanos en la regulacin de la respuesta
inmune adaptativa, en condiciones
patolgicas como autoinmundad y
cncer.

Dra. en Ciencias Biolgicas de la
Universidad de Buenos Aires.

DRA. CARLA ASSTEGIANO
CORREO ELECTRONICO
asteggianocarla@hotmail.com

POSICION ACTUAL:
Directora del laboratorio de
Desrdenes Congnitos de la
Glicosilacin en el Centro de Estudio de
las Metabolopatas Congnitas
(CEMECO), Profesora Adjunta de
Qumica en la Facultad de Medicina de
la Universidad Catlica de Crdoba.
Argentina

Estudio de glicoprotenas de membrana
plaquetaria humana, Bases moleculares
de displasias esquelticas debidas a
alteraciones de O-glicosilacin entre
otras o-glicanopatas).

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Doctorado en Ciencias Biolgicas con
formacin especialista en gentica
molecular humana y glicobiologa
humana

DATOS RELEVANTES:
Capacitacin en el estudio de los
Desrdenes Congnitos de la
glicosilacin (CDG) en centros de
investigacin europeos pertenecientes
a la red Euroglycanet.

DRA. LEILA M. LOPES-BEZERRA

CORREO ELECTRONICO:
lmlb23@globo.com

POSICION ACTUAL:

Profesora adjunta en la Universidad
Estatal de Ro de Janeiro.

Estudio de Sporothrix schenckii
causante de la esporotricosis, la
adhesin de los antgenos en la matriz
extracelular, pared celular, el endotelio,
y serodiagnstico con glicoconjugados.

Doctorado en Bioqumica por la
Universidad Federal de Ro de Janeiro
en 1991.

DATOS RELEVANTES:
Creadora de la primera vacuna sinttica
basada en carbohidratos

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DR. VICENTE VEREZ
CORREO ELECTRONICO:
vicente.verez@cqb.cu vicente@fq.oc.uh.cu

POSICION ACTUAL:
Director General del Centro de Qumica
Biomolecular. Laboratorio de Antgenos
sintticos. Facultad de Qumica,
Universidad de la Habana, Cuba. Centro
de Qumica Biomolecular

Vacunas sintticas de carbohidratos
antignicos para uso humano

Dr. En Qumica

DATOS RELEVANTES:
Premio en honor de William J Probst de
la Universidad del Sur de Illinois en
2006. Es miembro emrito de la
Academia de Ciencias de Cuba, miembro
de la Academia de Ciencias del tercer
mundo desde el 2007, miembro
honorario de la Sociedad
Latinoamericana de Glicobiologa

DR. TONY LEFEBVRE
CORREO ELECTRONICO
tony.lefebvre@univ-lille1.fr

POSICION ACTUAL:
Investigador Unit of Structural and
Functional Glycobiology (UGSF),
University of Lille
Glicobiologa estructural y funcional

Se gradu de la Universidad de
Minnesota, Twin Cities, de Minneapolis
en medicina interna.

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DATOS RELEVANTES:

DRA. VANNESA DEHENAUT.
CORREO ELECTRONICO
vanessa.dehennaut@ibl.fr

POSICION ACTUAL:
Investigador del Instituto de Biologa
de Lille, Universit de Lille Nord de
Francia

Modificaciones postraduccionales en
Cncer, estudio de la regulacin en las
funciones -catenina and ciclina D1
por O-GlcNAcylation

Licenciatura en biologa celular y
Fisiologa en la Universidad de Lille 1,
Francia .

Doctorado en ciencias Biolgicas en la
Universidad Lille 1, France , en el tema
Study of the tumor suppressor HIC1
(Hypermethylated in Cancer 1 .

Post- doctorado en el Institute of
Biology of Lille

Asistente de Profesor en la Universidad
de Lille 1, Francia

DATOS RELEVANTES: mltiples
artculos sobre glicoconjugados y O-
GlcNAcylation

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DR. TARIK ISSAD
CORREO ELECTRONICO
tajik.issad@inserm.fr

POSICION ACTUAL:
Director de Investigacin del Instituto
Cochin en Pars, INSRM

Regulacin de la insulina y el
metabolismo de la energa, mecanismos
celulares y moleculares.

Licenciatura y doctorado en Ciencias
Biolgicas en la Universidad de Pars.
Post-doctorado en la Universidad de
Bristol (Reino Unido).

DATOS RELEVANTES:
Pioneros en el desarrollo de la
tecnologa de BRET para el estudio de la
interaccin protena-protena en
clulas vivas. Se ha usado esta tcnica
para el estudio de la insulina y la
sealizacin de IGF-1

DR. BERNARD PRIEM
CORREO ELECTRONICO
priem@cermav.cnrs.fr

POSICION ACTUAL:
Investigador del "Centre de Recherches
sur les Macromolcules Vgtales.
Universidad de Grenoble, Francia

Ingeniera de carbohidratos de
microorganismos

Doctorado en Bioqumica en la
Universidad de Lille. Post-doctorado en

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Beltsville, Maryland, donde trabaj en
la maduracin del fruto de tomate

DATOS RELEVANTES:
Profesor en la Universidad de Grenoble,
investigacin en CERMAV, un conocido
centro CNRS en hidratos de carbono.

DR. ARIEL ORELLANA
CORREO ELECTRONICO:
aorellana@unab.cl

POSICION ACTUAL:
"Profesor Titular en la Universidad de
Concepcin, Chile, Doctor en Ciencias
Biolgicas, Biologa Celular y Molecular,
P. Universidad Catlica de Chile.
Departamento de Biologa, Universidad
de Chile"

Protemica, genmica y biosntesis de
polisacridos de pared celular de
plantas

DATOS RELEVANTES: "Investigador
principal durante el (2010-2014 ) en
Centro FONDAP de Regulacin del
Genoma
Investigador Principal. (2010-2013) en
Genmica de Plantas.
"

CONFERENCISTAS
NACIONALES

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DR. EDGAR ZENTENO GALINDO
CORREO ELECTRONICO:
ezenteno@servidor.unam.mx

POSICION ACTUAL: Jefe del Depto.
Bioqumica Fac. Medicina UNAM,
Investigador Nacional Nivel 3 y
Profesor Titular de Bioqumica y de
Inmunologa

LINEA DE INVESTIGACION: Papel
biolgico de los glicoconjugados en
diversas patologas y en respuesta
inmune innata.

FORMACIN PROFESIONAL: Mdico
Cirujano de la Facultad de Medicina
UNAM (1976), Doctor de Tercer Ciclo en
Bioqumica Aplicada (1986) y
Habilitacin para Dirigir la
Investigacin en el rea de Ciencias
Naturales (1994) de la Universidad de
Ciencias de Lille Francia.

DATOS RELEVANTES: Distincin Cecilio
Robelo, del Gobierno de Morelos
(1994). Mencin Honorfica en el
Premio CANIFARMA 1997 Dr. Alfredo
Tellez Girn, Premio CANIFARMA-
INFARVET en 2004. Profesor Honorario
por la Facultad de Medicina de la
Universidad Ricardo Palma de Per
(2007).

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DR. GABRIEL CUEVAS
CORREO ELECTRONICO: gecb@unam.mx

POSICION ACTUAL: Director del
Instituto de Qumica de la Universidad
Nacional Autnoma de Mxico

LINEA DE INVESTIGACION: Naturaleza
de los efectos estereoelectrnicos y de
las interacciones dbiles en la
conformacin, la reactividad y el
reconocimiento molecular

DATOS RELEVANTES: "Premio
Weizmann 1993 en Ciencias Exactas
Beca de la Fundacin Alexander von
Humboldt para efectuar estudios
posdoctorales.
Reconocimiento a los Autores
Mexicanos cuyos artculos han sido
frecuentemente citados a nivel
Internacional.
Institute for Scientific Information (ISI).
Premio de Investigacin 2002 de la
Academia Mexicana de Ciencias.
Distincin Universidad Nacional para
Jvenes Acadmicos en el 2003.
Investigador Nacional nivel III desde
2003."

CORREO ELECTRONICO:
everlope@ugto.mx

POSICION ACTUAL: Investigador Titular
Universidad de Guanajuato

LINEA DE INVESTIGACION: Mecanismos
de glicosilacin protenas de

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DR. EVERARDO LPEZ ROMERO
microorganismos patgenos

FORMACIN PROFESIONAL: Doctor en
Ciencias (Microbiologa), por el
Instituto Politcnico Nacional

DATOS RELEVANTES: realiz una
estancia sabtica en la UAMS
(University of Arkansas Medical School)
en Little Rock, AR
Sntesis de glicoprotenas en tejidos
animales. A su regreso a Guanajuato,
inici una lnea de investigacin sobre
los mecanismos de sntesis de estas
macromolculas utilizando hongos
(Cndida albicas) y protozoarios
(Entamoeba histolytica y E. invadens)
como modelos.

DR. IVAN MARTINEZ DUNCKER
CORREO ELECTRONICO:
duncker@uaem.mx

POSICION ACTUAL: Jefe del Laboratorio.
De glicobiologa humana. Profesor-
Investigador Universidad del Estado de
Morelos

LINEA DE INVESTIGACION: Glicosilacin
de protenas y su relacin con la pato
fisiologa molecular de diversas
enfermedades humanas

FORMACIN PROFESIONAL: Dr. en
Ciencias, Universidad de Paris,
Postdoctorado. Instituto Nacional de
Ciencias y Tcnicas Nucleares de
Francia.

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DATOS RELEVANTES: Presidente de la
Sociedad Latinoamericana de
Glicobiologa

DRA. LAURA PALOMARES
CORREO ELECTRONICO:
laura@ibt.unam.mx

POSICION ACTUAL: Investigadora
Titular del Instituto de Biotecnologa de
la UNAM

LINEA DE INVESTIGACION: Ingeniera
del cultivo de clulas animales para la
produccin, caracterizacin y
purificacin de glicoprotenas y
protenas multimricas recombinantes.

FORMACIN PROFESIONAL: Ingeniera
Bioqumica por el Instituto Tecnolgico
y de Estudios Superiores de Monterrey,
Investigadora Asociada en 1999
postdoctorado en la Universidad de
Cornell, EUA, doctora en Ciencias (1999)
en la UNAM.

DATOS RELEVANTES: Su trabajo
doctoral fue distinguido con la Medalla
Alfonso Caso 1999 al Mrito
Universitario que otorga la UNAM, el
Premio Alfredo Snchez Marroqun
2001 a la mejor tesis de doctorado
(otorgado por la Sociedad Mexicana de
Biotecnologa y Bioingeniera, SMBB) y
el Premio Weizmann-Khan 2001 a la
mejor tesis doctoral en investigacin
tecnolgica (otorgado por la Academia
Mexicana de Ciencias)

27

DR HECTOR MORA
CORREO ELECTRONICO:
hmora@ugto.mx

POSICION ACTUAL: Profesor-
Investigador Titular de la Universidad
de Guanajuato

LINEA DE INVESTIGACION: Estructura y
funcin de la pared celular de hongos
patgenos en reconocimiento inmune

DATOS RELEVANTES: Editor asociado
de la seccin de divulgacin cientfica
de la pgina de web " The International
Society for Human and Animal Mycology
, miembro del comit editorial de la
revista internacional Medical Mycology,
Editor en Jefe de la revista internacional
Journal of Glicobiology.

CORREO
ELECTRONICO: humberto@insp.mx

POSICION ACTUAL:
Director del rea de Infeccin e
inmunidad CISEI
Presidente de la Sociedad Mexicana de
Inmunologa

Evolucin y Filogenia de la Respuesta
Inmune, inmunidad innata, especies
reactivas de oxgeno e investigacin en
protemica.

Licenciado en Biologa. ENEP-Iztacala
UNAM. Doctor en Inmunologa, Escuela
Nacional de Ciencias Biolgicas, IPN.

28
DR. HUMBERTO LANZ
Posdoctorado en Inmunologa de
Insectos (Universidad de Estocolmo).
Posdoctorado en Entomologa Aplicada
(Escuela Politcnica de Zrich).
Posdoctorado en Inmunologa celular
de insectos (Universidad de Arizona).

DATOS RELEVANTES:
Investigador Nacional II
Presidente de la Sociedad Mexicana de
Protemica 2010-2011.

DRA. VICTORIA PANDO
CORREO ELECTRONICO:
victoria.pando@insp.mx

POSICION ACTUAL: Investigador en
Ciencias Mdicas "C. Unidad de
Protemica, Instituto Nacional de Salud
Publica

LINEA DE INVESTIGACION: interaccin
patgeno hospedero, principalmente
en el modelo del virus dengue-
monocitos/macrfagos

Licenciatura en Qumica, en la
Universidad Peruana Cayetano Heredia.
En el 2002, termin el Doctorado en
Ciencias en el Instituto de Biotecnologa
de la Universidad Nacional Autnoma
de Mxico.

DATOS RELEVANTES: Miembro
fundador y actual presidente de la
Sociedad Mexicana de Protemica

CORREO ELECTRONICO:
landapi@hotmail.com

29

DRA. LOURDES ARRIAGA

POSICION ACTUAL:
"Investigador Asociado D, Unidad de
Investigacin Mdica en
Inmunoqumica, Hospital de
Especialidades, Centro Mdico Nacional
Siglo XXI, Instituto mexicano del Seguro
Social. Miembro del sistema Nacional de
Investigadores Nivel I.

Marcadores inmunolgicos en
enfermedades inflamatorias sistmicas

Dra. En Ciencias Qumico-Biolgicas
por la ENCB.

DATOS RELEVANTES: Miembro del
sistema Nacional de Investigadores
Nivel I. Especialista en citometra de
flujo que utiliza la tcnica para la
bsqueda de inmunofenotipos con
potencial valor diagnstico/pronstico
en enfermedades inflamatorias
sistmicas. Encargada del Captulo de
Citometris de Flujo de la SMI

CORREO ELECTRONICO:
yobanper@gmail.com

POSICION ACTUAL:
Profesor-Investigador de la Universidad
Autnoma de Benito Jurez de Oaxaca

Glicosilacin de dominios lipdicos

30

DRA. YOBANA PEREZ

Doctora en Bioqumica de la Universit
des Sciences et Technologies de Lille,
FRANCE

DR. PEDRO HERNANDEZ CRUZ
CORREO ELECTRONICO:
fuegoblanco68@yahoo.com.mx

POSICION ACTUAL:

FORMACIN PROFESIONAL: Dr. Ciencias
Biomdicas UNAM

DATOS RELEVANTES: Investigador Nivel
I del Sistema Nacional de
Investigadores.

CONFERENCISTAS CURSO
PRECONGRESO
DRA. BLANCA ESPINOSA
CORREO ELECTRONICO:
bespinosa1118@yahoo.com.mx

POSICION ACTUAL: Profesor-
Investigador Depto. de Bioqumica INER

Glicosilacin en Alzheimer

Dra. Ciencias Biomdicas UNAM

31

DATOS RELEVANTES:
Investigador Nivel I del Sistema
Nacional de Investigadores Investigador
en Ciencias Mdicas, CCINSHAE

DR. MIGUEL ANGEL MAYORAL

CORREO ELECTRONICO:
mianmayo@yahoo.com

POSICION ACTUAL:

Glicobiologa del Cncer

Biomdicas UNAM

DATOS RELEVANTES:
Nacional de Investigadores.

CORREO ELECTRONICO:
rlascurainl@yahoo.com.mx

POSICION ACTUAL:
Profesor Titular -Investigador Facultad
de Medicina, UNAM

LINEA DE INVESTIGACION: Inmunologa
del asma alrgico

Biomdicas UNAM

32

DR. RICARDO LASCURAIN

DATOS RELEVANTES:
Investigador Nivel II del Sistema
Nacional de Investigadores. PDRIDE D

DR. ANTONIO SERRATO
CORREO ELECTRONICO:
serratoiner@gmail.com

POSICION ACTUAL:
Profesor-Investigador Depto. de
Bioqumica INER

Glicobiologa de anticuerpos

Doctor en Biotecnologa, UNAM

DATOS RELEVANTES:
Nacional de Investigadores Investigador
en Ciencias Mdicas, CCINSHAE

33
RESUMEN DE CONFERENCIAS
MAGISTRALES

SESION GENERAL 1 CONFERENCIAS INAGURALES
AUDITORIO DR. GUSTAVO BAZ PRADA
Coordinador: Dr. Ivn Martnez Druncker

BIOLOGICAL ROLES OF GLYCANS - A LOOK BACK OVER THE DECADES
Ajit Varki
Glycobiology Research and Training Center, Departments of Medicine, and Cellular & Molecular
Medicine, University of California at San Diego, La Jolla, CA, USA

Both simple and complex glycans have long been known to play major metabolic,
structural and biophysical roles in various biological systems. Pathogen and commensal
recognition of host glycans has also been the subject of intense study for many
decades. However such biological roles cannot fully explain the remarkable complexity
and organismal diversity of glycan structures found in nature. Indeed, in systematically
reviewing the subject just twenty years ago (Glycobiology 3:97-130, 1993), one had to
search hard to find a few clear-cut instances of more specific biological roles of glycans
that are of intrinsic value to the organism that synthesizes the glycans. In striking
contrast there is now a wealth of examples to choose from. Thus, any current review of
the biological roles of glycans cannot possibly be comprehensive. Instead, a historical
overview will be presented, broad principles outlined and a few specific examples cited,
representing biological roles of different kinds mediated by various classes of glycans,
in different evolutionary taxa. What has remained unchanged since 1993 is the fact that
while all of the theories regarding biological roles of glycans are supported by

34
compelling evidence, exceptions to every single one can also be found. In retrospect
this is not too surprising. Complex and diverse glycans are now known to be ubiquitous
to all living cells in nature, and essential to all life forms. Thus, it was inevitable that >3
billion years of evolution selected these molecules for several key biological roles even
while using them in other circumstances for relatively unimportant roles, or sometimes
even expressing them for no obvious roles at all. In this respect glycans are no different
from other classes of biological macromolecules such as DNA, RNA, proteins and lipids.
The question now to be addressed is, what biological roles do glycans not mediate?

TOOLS FOR IDENTIFYING NEW HUMAN GLYCOSYLATION DISORDERS
Hudson Freeze, Sanford-Burnham. Medical Research Institute, La Jolla CA

Today there are nearly 100 human glycosylation disorders caused by mutations in
genes serving one or more biosynthetic pathways. In 2013 alone, a new glycosylation
disorder was reported on average every 11.7 days. That trend is likely to continue in the
near future since at least 2% of the human genome encodes genes used for glycan
biosynthesis and recognition. Additional unanticipated genes will certainly be identified
that affect glycosylation pathways or alter glycan structure. Evidence of their importance
will come from the discovery of patients who carry function-compromising mutations in
these genes.
This bonanza of discovery was fueled and enabled by a coalescence of genomic,
biochemical and social media technologies that include all stakeholders: scientists,
physicians, patients themselves, and their families.
The falling costs and greater availability of exome and genome sequencing will soon
make it an accessible diagnostic clinical test. Sophisticated data analysis programs filter
and distill terabytes of sequencing data to a few candidate genes. The key word is
candidate. Functional validation of putative mutations is needed to confirm the
predictions of a mutations deleterious impact. The well-considered and sophisticated
filters may sometimes inadvertently dismiss critical candidates. An example of such an

35
oversight is the identification of glycosylation disorder due to spontaneous mutations in
4 patients resulting in somatic mosaicism. Standard exome analysis overlooked the
defective gene, but routine analysis of a glycan-based biomarker, transferrin, provided
the critical biochemical evidence early on that identified the gene. A combination of
reliable biochemical tests and exome/genome sequencing is indispensible. It enabled
us to identify 8 new glycosylation disorders from 50 patients who showed abnormal
glycosylation. Moreover, incoming results of exome sequencing in patients for whom
glycosylation markers were not analyzed deliver new glyco-candidates that can be
validated by glycosylation analysis of serum, fibroblasts or lymphoblasts.

Today, the highly motivated parents of children with unknown metabolic disorders scour
the web and social networks on a daily search for leads and answers for their families.
Our laboratories are but a click away from their questions and their cooperation. As new
genes are discovered that reveal causes of the estimated 3500 unsolved genetic
disorders, the basic scientist will lead the assault on functional analysis of these genes
with support from an increasingly savvy group of web-wise families who are fully
committed to solutions. This creates an opportunity to educate and strengthen the vital
and expanding links between basic scientists, physicians and the voter/tax payer
families. Solving glycosylation disorders provides a model of how to integrate and
advance both medical and fundamental science to benefit afflicted families.
Supported by R01DK55615 and The Rocket Fund.

36
SYMPOSIUM
TECNICAS PARA EL ESTUDIO DE LA GLICOBIOLOGIA

BIOINGENIERIA APLICADA (NUCLEASAS CON DEDOS DE ZINC Y
MODIFICACIONES DE GENOMAS EN SISTEMAS EUCARIOTICOS.
MsC David Pierre Pillon.
Sigma Aldrich de Mxico. David.Pillon@sial.com

Las nucleasas con dedos de zinc (ZFN) son una clase de protenas de unin a ADN,
derivadas de la bioingeniera, que facilitan la edicin especfica del genoma mediante la
creacin de rupturas de la doble cadena del ADN en los lugares especificados por el
usuario. Las rupturas de la doble cadena son importantes para la mutagnesis sitio-
especfica que estimula los procesos naturales de reparacin del ADN de las clulas,
es decir la recombinacin homloga y la unin de extremidades no homologas (NHEJ).
La ingeniera genmica en clulas de mamfero presenta un enorme potencial para
investigacin bsica, descubrimiento de medicamentos, as como para la produccin
de medicamentos basados en tcnicas de cultivo celular.
Para este fin, Sangamo Biosciences y Sigma-Aldrich se han asociado recientemente
para desarrollar y proveer una nueva tecnologa que permite la edicin del genoma a
travs del diseo de nucleasas con dedos de zinc (ZFN). Dentro de estas protenas
quimricas, la especificidad de unin al ADN es determinada por la protena con
dedos de zinc permitiendo definir el sitio de accin de la nucleasa. Tales Nucleasas
(ZFN) son capaces de reconocer y unirse a un lugar especfico del genoma y provocar
un corte preciso de la doble cadena del ADN con alta eficiencia y precisin. La clula
entonces emplea los procesos naturales de reparacin del ADN, como son la
"reparacin homologa dirigida (HDR)" o "unin de extremidades no homlogas (NHEJ)"
para reparar de manera especfica el punto de ruptura. Estas dos vas proporcionan al
investigador la capacidad de provocar tres opciones claras para editar genomas:
correccin de genes, supresion de genes y la adicin dirigida de genes o secuencias de
inters. Por otra parte, la velocidad y la eficiencia de este proceso nos permiten realizar
knock-out (KO) en mltiples genes en la misma clula. El tener acceso a la ingeniera
gentica facilita la creacin de lneas celulares y animales transgnicos para
aplicaciones en investigacin biomdica, generando un nuevo campo de oportunidades
para el desarrollo de nuevos frmacos, pero sobre todo la posibilidad de seguir el
camino de la medicina personalizada.

37
La tecnologa de las ZFN pone al alcance de los investigadores una herramienta mucho
ms precisa, acelerando la investigacin cientfica y brindando as mayores
oportunidades de desarrollo de terapias an ms eficaces y personalizadas

USO DE LA ESPECTROMETRA DE MASAS PARA LA IDENTIFICACIN DE
RESIDUOS DE CARBOHIDRATOS
Dra. Victoria Pando-Robles
Centro de Investigacin en Enfermedades Infecciosas, Instituto Nacional de Salud Pblica.

La espectrometra de masas (EM) es una tcnica analtica que se ha convertido en el
eje central de la investigacin a nivel de protenas y biomolculas. La capacidad de
identificar protenas y determinar su estructura primaria es fundamental para el estudio
de los seres vivos, donde la secuencia de aminocidos comprueba la conexin entre
protena y su gen codificante, va el cdigo gentico; mientras que el descubrimiento de
sus modificaciones post-traduccionales hace evidente la relacin entre fisiologa celular
y gentica. Los espectrmetros de masas existen desde 1900, miden la relacin
masa/carga de las molculas. Sus componentes principales son: la fuente de
ionizacin, el analizador de masas y el detector, que existen en varias versiones, la
combinacin de estas partes es muy importante ya que repercute en la sensibilidad y
exactitud de la medicin. A fines de los 80s se desarrollaron fuentes de ionizacin
suave, que permitieron el anlisis de polmeros como las protenas, los cidos
nucleicos y los carbohidratos.
Los proteoglicanos (PG) son una clase especial de glicoprotenas altamente
glicosiladas, formados por varios ncleos proteicos que se modifican post-
traduccionalmente con polisacridos denominados glicosaminoglicanos (GAG), que a
su vez son polmeros de carbohidratos lineares cargados negativamente. Dada su
diversidad estructural los proteoglicanos realizan diferentes funciones tanto en la
matriz extracelular como en la clula, y han sido involucrados en diferentes procesos
celulares: interacciones clula-clula, clula-matriz, activacin de quimiocinas y
citocinas, morfognesis de tejidos durante el desarrollo embrionario, migracin,
proliferacin celular y en el reconocimiento de patgenos.
Proteoglycomics se refiere al estudio de los proteoglicanos usando las herramientas
del anlisis protemico. Las funciones individuales de los PG pueden ser atribuidas
tanto al centro proteico como a las cadenas GAG, donde la determinacin de la
estructura GAG es un reto analtico formidable debido a su complejidad estructural, su
carga negativa, polidispersidad y microheterogeneidad.

THE INTERACTION CH / PI, PROTEIN CARBOHYDRATE RECOGNITION BASE
AND ITS APPLICATION TO THE SYNTHESIS OF CATALYSTS.
Fabin Cutara Guadarrama and Gabriel Cuevas

38
Instituto de Qumica. Un iversidad Nacional Autnoma de Mxico. Apdo. Postal 70213,
04510, Coyoacn, Circuito Exterior, Mxico D.F. Mxico.

Specific interactions between molecules, including those produced by a given solute,
and the surrounding solvent are essential to drive molecular recognition processes. A
simple molecule such as benzene is capable of recognizing and differentiating among
very similar entities, such as methyl 2,3,4,6- tetra-O-methyl--D-galactopyranoside ( -
Me5Gal), methyl 2,3,4,6-tetra-O-methy--D-galactopyranoside (-Me5Gal), 1,2,3,4,6-
penta-O-acetyl- -D-galactopyranose (-Ac5Gal), and methyl 2,3,4,6-tetra-O-methyl- -
D- mannopyranoside (-Me5Man). In order to determine if these complexes are formed,
the interaction energy between benzene and the different carbohydrates was
determined, using Calvet microcalorimetry, as the enthalpy of solvation. These enthalpy
values were -89.0 ( 2.0, -88.7 ( 5.5, -132.5 ( 6.2, and -78.8 (3.9 kJ mol-1 for the four
complexes, respectively. Characterization of the different complexes was completed by
establishing the molecular region where the interaction takes place using NMR. It was
determined that -Me5Gal is stabilized by the CH/ interaction produced by the
nonpolar region of the carbohydrate on the face. In contrast, -Me5Man is not
specifically solvated by benzene and does not present any stacking interaction.
Although -Me5Gal has a geometry similar to that of its epimer, the obtained NMR data
seem to indicate that the axial methoxy group at the anomeric position increases the
distance of the benzene molecules from the pyranose ring. Substitution of the methoxy
groups by acetate moieties, as in -Ac5Gal, precludes the approach of benzene to
produce the CH/ interaction. In fact, the elevated stabilization energy of -Ac5Gal is
probably due to the interaction between benzene and the methyl groups of the acetyls.
Therefore, methoxy and acetyl substituents have different effects on the protons of the
pyranose ring.
With these data on hand it is possible to establish that benzene can recognize
galactose and this property can be combined with the catalytical properties of
phenylpyridone to promote esther aminolysis reactions. Both effects can be used to
generate a molecule that recognizes a region of galactose and transforms an acetyl
group. The substrate requires the incorporation of an acetyl group in the anomeric
position and introduces methyl groups to eliminate the formation of hydrogen bonds
which are dominant when compared with the CH/ interaction. The molecule was
modeled using computational methods at the M05-2x/6-31+G(d,p) level; and was
synthesized using a procedure that needs 3 steps and renders an 83% yield. The
substitution of the phenyl group with a cyclohexane slows down the hydrolysis from
0.039 0.005 l
2
mol
-2
hr
-1
to 0.028 0.003. The homologation of the chain gives a rate of
hydrolysis of 0.028 0.003. NMR was used to demonstrate the interaction between the
catalyzers phenyl group and galactose protons at positions 3, 4 and 5. The results
show that CH/ interactions participate in the recognition processes between
carbohydrates and aromatic groups of certain amino acids. This contribution adds to the
existing evidence of the usefulness of such interactions. This can be used to develop
organocatalyzers that can contribute to streamline diverse chemical processes.

39
SESION GENERAL 2 GLICOBIOLOGIA MEDICA
Coordinador: Dr. Hctor Mora

GLYCOBIOLOGY IN SKELETAL DYSPLASIA DUE TO CONGENITAL DISORDERS
OF GLYCOSYLATION (CDG)
Carla G Asteggiano (PhD)
Investigadora Adjunta CONICET (Consejo Nacional de Investigaciones Cientficas y Tcnicas),
Argentina.
Directora Laboratorio Desrdenes Congnitos de las Glicosilacin, Centro de Estudio de las
Metabolopatas Congnitas (CEMECO), Facultad de Ciencias Mdicas, Universidad Nacional de
Crdoba, Crdoba, Argentina.
Profesora Adjunta, Facultad de Medicina, Universidad Catlica de Crdoba, Crdoba, Argentina.

E-mail: asteggianocarla@hotmail.com Web page: www.cdgargentina.com.ar
Glycosylation diseases are due to defects in different pathways, in protein glycosylation
and, recently defects in glycolipid have been identified as well. Congenital Disorders of
Glycosylation (CDG) are rapidly growing genetic pathologies with more than 50 genes
reported since 1980, the first clinical description by Prof. Jaak Jaeken. Defects have
been identified in N-glycosylation and O-glycosylation pathways and combined N- and
O-glycosylation types of CDG. The clinical manifestations can affect nearly all organs
and systems or could be restricted to a specific tissue such as the cartilage in multiple
osteochondromatosis (MO or EXT1/EXT2-CDG). An important neurological component
is often reported but a peculiar skeletal phenotype has been described in CDG patients,
gaining special relevance over the past few years. In patients exposed to the cell
hypoglycosylation due to altered glycosylation pathway, numerous extracellular matrix
proteins undergo glycosylation defects that lead to skeletal manifestations.
During the last eight years, we have focused on CDG and we detected altered N-
glycosylation (2 PMM2-CDG and 4 CDG-IIx patients) and O-glycosylation (33
EXT1/EXT2-CDG patients) subtypes. Most of CDG are autosomal recessive, but
EXT1/EXT2-CDG was described as a dominant disease. It is characterized by short
stature and development of benign bone tumors during childhood and adolescence. It is
based on heterozygous mutations in EXT1 or EXT2, two genes that codified
glycosyltransferases required for the synthesis of heparin sulfate proteoglycans

40
(HSPGs). This special pathology and other with a severe skeletal phenotype have
called our interest: O-glycosylation defects as GALNT3-CDG (Hyperfosfatemic Tumoral
Calcinosis); LFNG-CDG (Spondylocostal Dysostosis); SLC35D1-CDG
(Schneckenbecken Dysplasia); B4GALT7-CDG (Progeroid Variant of Ehlers Danlos)
and B3GALTL-CDG (Peter-Plus Syndrome). In these pathologies skeletal
manifestations as osteopaenia, rib cage abnormalities, short stature, kyphosis and
scoliosis are the most common manifestations. Molecules dependent on post-
translational modification processes such as glycosylation may play specific functions in
bone mineralization and in the endochondral growth plate. The glycosaminoglycans
heparan sulfate (HS), are usually found in the cell surface and extracellular matrix as
HSPG. They play an integral role in the structural integrity of various tissues, in ligand
binding, cell adhesion and cell signaling during endochondral ossification, process
regulated by growth factors that bind to HS chains.
Different reports postulated that it is very probable that not only hypoglycosylation but
also gain-of-glycosylation may cause skeletal phenotypes. Our results highlight the
hypoglycosylation effect on the genesis of skeletal manifestation in CDG patients,
suggesting that the process of glycosylation is also important in proteins implicated in
the development of cartilage and bone. In conclusion, it is strongly recommended to
search CDG diseases in any unexplained skeletal dysplasia. PICT2010/2824-UCC
2011-2013, CONICET.

SESION GENERAL 3
GLYCOBIOLOGY OF INFECTIOUS DISEASES
Coordinador: Dr. Jos Luis Montiel

Candida albicans CELL WALL GLYCOBIOLOGY.
Mora-Montes Hctor M.*, Flores-Carren Arturo, Daz-Jimnez Diana F., Gonzlez-Hernndez Roberto
de J., Tamez-Castrelln Alma K. Departamento de Biologa, Universidad de Guanajuato, Noria Alta s/n,

41
Col. Noria Alta, C.P. 36050, Guanajuato, Gto., Mxico. Tel. 473 732 0006, ext. 8154. *e-mail:
hmora@ugto.mx

The cell wall of the opportunistic pathogen Candida albicans -
glucans and glycoproteins, which are enriched with N-linked and O-linked mannans.
Protein mannosylation starts in the endoplasmic reticulum and finalizes in the Golgi
complex, where mannosyltransferases further modify the mannans. The study of
glycosylation pathways in C. albicans has allowed assessing the importance of these
protein modifications for fungal virulence and interaction with the host immune system.
We have generated a collection of mutant cells lacking key steps in N-linked
mannosylation processes and have found that disruption of both N-linked mannan core
trimming and outer chain extension, are required for virulence, proper cell wall
composition, dimorphism, and interaction with components of the immune system;
though the disruption of N-linked mannosylation pathway led to increased virulence in
C. glabrata. Over the last years, we have focused to the study of two
mannosyltransferase gene families: MNT1 and MNN4. Using genetic and biochemical
approaches we have found that the members of the MNT1 gene family have redundant
roles in the biosynthesis of phosphomannan, N-linked and O-linked mannans. In
addition, the encoded enzyme activities are required for a proper cell wall structure,
virulence and immune sensing. Members of the MNN4 gene family encode both
phosphomannosyltransferases and positive regulators of such enzyme activity, and
together with members of the MNT1 gene family participate in elaboration of
phosphomannan, a cell wall component required for phagocytosis and the effect of
antimicrobial peptides.

This work is supported by CONACyT (CB2011-166860) and Universidad de Guanajuato.

42
SESION GENERAL 4 GLICOBIOTECNOLOGIA
Coordinador. Dr. Everardo Lpez Romero

N-GLYCOSYLATION DETERMINES THE STABILITY AND IMMUNOGENICITY OF
RECOMBINANT INFLUENZA HEMAGGLUTININ.
Laura A. Palomares, Lus ngel Cueto, Vanessa Hernndez, Ana Ruth Pastor and
Octavio T. Ramrez
Instituto de Biotecnologa, Universidad Nacional Autnoma de Mxico. Av. Universidad
2001 Col. Chamilpa, Cuernavaca Morelos. C.P. 62210, Mxico. Tel. (777) 3291646,
laura@ibt.unam.mx

Hemagglutinin (HA) is the main antigen of influenza virus. Antibodies against HA block
viral infection. Recombinant HA is an efficient vaccine against influenza virus infection.
In this work, we investigated the role of N-glycosylation of HA in its susceptibility to
aggregation. HA was produced in the insect cell-baculovirus system, purified and
deglycosylated with Endo-H. Deglycosylated HA aggregated when exposed for 10 min
to temperatures above 45C. In contrast, glycosylated HA only aggregated at
temperatures above 55C. Glycosylated and deglycosylated HA were more stable at
pH 6.8, but while few aggregates were observed in glycosylated HA, 20 % of
deglycosylated HA was aggregated. Changing the pH between 3 and 8 resulted in
different extents of aggregation, ranging from 2 to 18% in glycosylated HA and from 18
to 50% in deglycosylated HA. The kinetics of aggregation caused by temperature or pH
were different. Aggregation caused by temperature followed an exponential increase
with time, indicating a cooperative aggregation. No difference in the aggregation rates
at the temperatures tested was observed. Aggregation caused by pH followed kinetics
independent of the aggregate concentration, and higher aggregation rates were
observed as pH decreased. Finally, the immunogenicity of glycosylated and
deglycosylated HA was measured in mice. It was found that immunizing with
deglycosylated HA resulted in IgG titers 20 times higher than those obtained when
glycosylated HA was used. It was demonstrated that N-glycosylation of HA determines
protein stability and immunogenicity.

DEVELOPMENT OF Streptococcus pneumoniae CONJUGATE VACCINE WITH
THE MOST PREVALENT SEROTYPES IN LATIN AMERICA.
Y. Valds Balbin
1
, D. Santana Medero
1
, D. Garca Rivera
1
, A. Villar Aneiros
1
, D. Gonzlez Daz
2
, B. Paredes Moreno
1
, L. Rodrguez Noda
1
, U. Ramrez Gonzlez
2
, J. Chang Caldern
1
, J.
Lora Garca
1
, V Verez Bencomo
1
.

43
1
Center for Biomolecular Chemistry, calle 21 y 200, Playa and
2
Finlay Institute Ciudad
Habana, Cuba

The development of a conjugate vaccine against multiple serotypes of S. pneumonia is
a complex task with numerous challenges. Several years ago, the Center for
Biomolecular Chemistry launched a research project for developing a heptavalent
vaccine containing the seven serotypes of S. pneumoniae more frequently associated
with infection in Cuba and Latin America. As a result we were able to design a
candidate vaccine containing 2g of each capsular polysaccharide 1, 5, 14, 18C, 19F
and 23F as well 4g for 6B - all of them conjugated to tetanus toxoid- and aluminum
phosphate as adjuvant. After several years of research and development, the
technology was established for the production of the seven active pharmaceutical
ingredients and of the combined vaccine. Several batches have been produced with the
aim to assess their physico-chemical properties and to perform the preclinical and
toxicological studies, guaranteed by a Quality Assurance System
1
. Last year, the first
clinical trial was conducted, demonstrating the safety and immunogenicity of vaccine in
healthy young. Presently, we are beginning the clinical trials in infants, with focus on
Phase I and Phase II no inferiority; thus paving the way toward first Latin-American
custom-designed conjugate vaccine against S. pneumoniae.

* Dedicated to Violeta Fernndez Santana memory. Deceased on 20
th
November, 2011
1 D. Garca-Rivera, L. Rodrguez, G. Hernndez, et al,8
TH
International Symposium
Pneumococci and Pneumococcal diseases ISPPD-8, 104, 2012

CARBOHYDRATE ENGINEERING IN Escherichia coli K-12.
Priem Bernard. CNRS-CERMAV, UPR 5301, BP53X, 38041 Grenoble cedex 9, France Tel +33
476037648. priem@cermav.cnrs.fr.

44
Bacterial metabolic engineering has emerged as a powerful method for large scale
synthesis of oligosaccharides. Glycosylation reactions are performed by whole cells
overexpressing the genes encoding the appropriate glycosyltransferases and sugar-
nucleotide biosynthesis. Combinations of engineered strains carrying several plasmids
allow producing a great variety of mammal carbohydrate structures. Furthermore,
chemical modifications can be carried out before or after the bacterial cultures, thus
allowing obtaining chemically modified oligosaccharides. Those chemical modifications
can be used either to ease chemical couplings or to modify the biological properties of
carbohydrates. An overview of the technology and suitable examples will be presented
in the talk.

SESION GENERAL 5
BIOSINTESIS Y METABOLISMO DE GLICOCONJUGADOS

FUNCTIONS PLAYED BY O-GlcNACYLATION
Tony Lefebvre, Professor, Lille 1 University, CNRS/UMR 8576, Unit of Structural and Functional
Glycobiology, Villeneuve d'Ascq, FRANCE

Post-translational modifications (PTM) regulate protein functions by promoting or
preventing protein-protein interactions (theory of "one partner-one function"). The
modification therefore re-localizes the protein, impacts on its expression or regulates
enzyme activity as shown recently for PFK1. Glycosylations is a large family of PTM
that includes O-GlcNAcylation maybe the simplest one at the structural point of view.
O-GlcNAcylation consists in the reversible addition of a single N-acetylglucosamine to
the hydroxyl groups of serine and threonine of cytosolic and nuclear proteins. O-
GlcNAcylation processes are managed by a unique couple of enzymes namely O-
GlcNAc transferase (OGT) responsible for the transfer of the GlcNAc moiety and O-
GlcNAcase (OGA) that reverses the reaction. Despite appearances, O-GlcNAcylation
hides a very complex regulation mode such as phosphorylation with which besides O-

45
GlcNAcylation could either competes or acts in concert. In this way, O-GlcNAcylation is
part of signaling pathways which control the level of the glycosylation and in turn O-
GlcNAcylation interferes with the regulation of the PI3K and MAPK pathways. More
especially, in response to insulin OGT is targeted to lipid microdomains in a PI3K-
dependent manner. O-GlcNAcylation levels intimately correlate with the nutritional
status of the cell due to the nature of the nucleotide-sugar, UDP-GlcNAc, from which it
comes from. Therefore O-GlcNAcylation relays nutrient and energy status to cell
homeostasis. A -catenin O-GlcNAcylation, which
reduces its proteasomal susceptibility, is responsible for epithelial tumorigenesis. This
explains partly why patients suffering diabetes and obesity are more inclined to develop
colorectal cancers. In another set of studies, we showed that O-GlcNAcylation is crucial
for cell cycle progression. OGT is required for quiescent cells to reach the G0/G1
transition and for Xenopus laevis oocytes to mature in a process similar to G2/M
transition. On the contrary, OGA activity correlates with G1/S transition. On a
transcriptional point of view, it has been recently show by several groups that OGT
cooperates with TET proteins to remodel chromatin, a process needed for gene
expression. The focus of this talk is to give precise examples in which OGT and O-
GlcNAcylation are pivotal elements for cell fundamental processes.

NOVEL REGULATION OF POSTTRANSLATIONAL MODIFICATIONS IN THE
GOLGI APPARATUS: FROM BASIC SCIENCE TO DESEASES.
Carlos B. Hirschberg, Boston University, Boston, MA, USA.

Nucleotide sugars are transported by specific transporters from the cytosol, their site of
synthesis, into the lumen of the Golgi apparatus and endoplasmic reticulum, where they
serve as substrates for glycosylation reactions. These transporters play critical roles in
glycosylation of proteins, lipids and proteoglycans, which are essential for
organogenesis, development, mammalian cellular immunity and pathogenicity of human
pathogenic agents. Given their essential roles it is not surprising that these transporters
have been identified in all eukaryotes examined so far. The biochemical characteristics
and biological significance of these transporters have been demonstrated in mammals,

46
Caenorhabditis elegans, Drosophila melanogaster, yeast fungi, Leishmania, Entamoeba
and recently Trypanosoma brucei and Toxoplasma gondii .Importantly, mutations in
these transporters, result in human diseases such as Leukocyte Adhesion Deficiency II
and Schneckenbecken dysplasia and well as Complex Vertebral Malformation in
bovines.
Over the past years, numerous biochemical studies have demonstrated that
glycoconjugates were deficient in the particular sugar for which the corresponding
nucleotide sugar transport was defective. However, our recent studies of silencing
nucleotide sugar transporter genes of mammalian cells showed a more global defect in
the synthesis and secretion of not only glycoproteins but also in non glycoproteins. The
latter is probably the result of endoplasmic reticulum stress induction and protein
translation inhibition and may explain some of the diversity of symptoms in mammalian
diseases caused by transporter mutations. Further in depth studies of transporter
structures, functions and their regulation of glycosylation in mammals should lead to a
better understanding of the molecular mechanisms underlying the above diseases and
facilitate the discovery of appropriate therapies.

UDP-GLUCOSE TRANSPORTERS AND THEIR ROLE IN PROTEIN QUALITY
CONTROL IN THE ENDOPLASMIC RETICULUM
Dr. Ariel Orellana
FONDAP Center for Genome Regulation, Centro de Biotecnologa Vegetal, Universidad Andrs Bello,
Santiago, Chile

Nucleotide sugars are the substrates for glycosyltransferases located in the ER and the
Golgi apparatus. Most of them are synthesized in the cytosol, therefore they are
incorporated into the lumen of these organelles by nucleotide sugar transporters
(NSTs). The NSTs are a large gene family composed by around 50 members in
Arabidopsis thaliana. We have found two UDP-glucose transporters (UTr1 and Utr3),
located in the ER. Both genes are targets of the unfolded protein response (UPR) and
mutants on each of them trigger UPR. Double mutants are not viable. Our data suggest

47
that these NSTs are supplying the substrate for UGGT, the glucosyltransferase that
recognize unfolded proteins in the ER. This is an enzyme we have detected in
Arabidopsis and the analysis of different mutant alleles shows that these plants are
smaller and sensitive to different stress.
The human ortholog of Utr1 and Utr3 is HuGTRel1. We have tested the activity of this
protein and found that transport UDP-glucose. This gene also responds to UPR. All
these results support the hypothesis that transport of UDP-glucose is an important
process in the folding of glycoproteins in the ER.

Supported by Fondap-CRG 15090007, ICM P10-062-F, PB-16 and DI-365-13/R

O-GlcNACYLATION, DIABETES AND CANCER
Dr. Tarik Issad. INSERM U1016, CNRS UR 8104, Institut Cochin, Paris, France. E-mail:
tarik.issad@inserm.fr

O-GlcNAc glycosylation (O-GlcNAcylation) corresponds to the addition of N-
acetylglucosamine on serine and threonine residues of cytosolic and nuclear proteins.
O-GlcNAcylation is a dynamic post-translational modification, analogous to
phosphorylation, that regulates the stability, activity or subcellular localisation of target
proteins. This reversible modification depends on the availability of glucose and
therefore constitutes a powerful mechanism by which cellular activities are regulated
according to the nutritional environment of the cell. O-GlcNAcylation has been
implicated in important human pathologies including Alzheimer disease, type-2 diabetes
and cancer. Only two enzymes, OGT and O-GlcNAcase, control the O-GlcNAc level on
proteins. Therefore, O-GlcNAcylation cannot organize in signalling cascades as
observed for phosphorylation. O-GlcNAcylation should rather be considered as a
rheostat that controls the intensity of the signals travelling through different pathways
according to the nutritional status of the cell.

48
Several lines of evidence indicate that O-GlcNAcylation could contribute to the
glucotoxicity phenomenon in diabetic patients, in which hyperglycemia induces
alterations that result in worsening of the glycemia.
Excessive production of glucose by the liver is a major cause of fasting hyperglycemia
in type 2 diabetes. We previously showed that O-GlcNAcylation of FoxO1 in liver cells
stimulated its transcriptional activity and increased the expression of genes involved in
hepatic glucose production, suggesting a potential role of FoxO1 O-GlcNAcylation in
glucotoxicity.
FoxO1 also plays a major role in the regulation of pancreatic -cell physiology. We
observed that increased O-GlcNAcylation of FoxO1 in pancreatic -cells stimulated its
transcriptional activity towards Insulin-like growth factor-binding protein-1 gene (Igfbp1),
resulting in increased expression and secretion of IGFBP1. Increased IGFBP1 secretion
inhibited IGF1/IGF1R signalling pathway, and reduced the activity of the Phosphatidyl-
Inositol-3-Phosphate Kinase/Akt (PI3K/Akt) pathway. We therefore propose a novel
glucotoxicity mechanism in which O-GlcNAcylation of FoxO1, through increased
IGFBP1 production, negatively regulates the PI3K/Akt signalling in -cells.
Numerous epidemiological studies indicate that obesity or diabetic conditions increase
the risk of cancer. Recent evidences suggested that O-GlcNAcylation may affect the
growth of cancer cells. However, the consequences of O-GlcNAcylation on anti-cancer
therapy had not been evaluated. Breast cancer is the most common cancer in women.
Anti-estrogen therapies (e.g., tamoxifen) have permitted important progress for the
treatment of hormone-sensitive breast cancers. However, the development of treatment
resistance constitutes an important limitation to these therapies. We recently studied the
effect of O-GlcNAcyaltion on tamoxifen-induced MCF-7 breast cancer derived cells. We
observed that treatments that increase O-GlcNAcyaltion markedly inhibit tamoxifen-
induced MCF-7 cell death, suggesting that targeting this pathway could constitute an
interesting strategy for the treatment of tamoxifen-resistant tumours.

49
SESION GENERAL 6
MODULACION DE LA RESPUESTA INMUNE Y GLICOBIOLOGIA DEL CANCER
Coordinador: Dr. Miguel Mayoral

GALECTINS IN HOST-PATHOGEN INTERACTIONS AND CANCER: STRUCTURAL
AND FUNCTIONAL ASPECTS
Dr. Gerardo VASTA . University of Maryland,Baltimore, EUA.
Department of Microbiology and Immunology, University of Maryland School of
Medicine, Institute of Marine and Environmental Technology, Baltimore, Maryland, USA

Galectins are characterized by their binding affinity for -galactosides, a unique binding
site sequence motif, and wide taxonomic distribution and structural conservation in
vertebrates, invertebrates, protista, and fungi. Since their initial description, galectins
were considered to bind endogenous (self) glycans and mediate developmental
processes, including cell differentiation and tissue organization or regeneration. In the
past few years, however, numerous studies have described the diverse regulatory
effects of galectins on immune homeostasis and cancer. More recently, however,
evidence has accumulated to support the notion that galectins also bind exogenous
(non-self) glycans on the surface of potentially pathogenic microbes, parasites, and
fungi, suggesting that galectins could function as pattern recognition receptors (PRRs).
According to the currently accepted model for non-self recognition, PRRs recognize
pathogens via highly conserved microbial surface molecules of wide distribution such as
LPS or peptidoglycan (microbe-associated molecular patterns; MAMPs), which are
absent in the host. Galectins, however, can bind similar self/non-self molecular patterns
on host and microbial cells. Furthermore, although some galectins can bind and kill
bacteria, thereby displaying activity as innate immune recognition and effector factors, it
appears that in most cases galectin-mediated recognition favors the potential pathogen

50
rather than the host. Therefore, some galectins do not fit the definition of PRRs,
underscoring the significant gaps in our knowledge about the structural and functional
diversity, subcellular targeting, localization, and secretion of the galectin repertoire
components in any given species, and the host-parasite co-evolutionary processes that
have resulted in such interactions (Supported by Grant 5R01GM070589 from NIH, and
IOS 1050518, IOB- 0618409 and IOS0822257 from NSF).

A ROLE FOR GALECTIN-GLYCAN INTERACTIONS: LINKING TUMOR
NEOVASCULARIZATION AND IMMUNITY
Marta A Toscano, PhD. Laboratorio de Inmunopatologa, Instituto de Biologa y Medicina Experimental
(IBYME/ CONICET). E-mail: martalitos@yahoo.com.ar

Recent efforts towards decoding the glycan signature of immune cells have revealed
dramatic changes in N- and O-glycan structures during immune cell maturation,
activation and differentiation. The responsibility of deciphering these glycosylation
changes is assigned in part to endogenous lectins which expression is dynamically
regulated during chronic inflammatory responses. We will discuss recent findings from
our laboratory demonstrating the contribution of glycosylation-dependent mechanisms
and galectin-glycan interactions to the regulation of a broad range of immunological and
vascular programs, including: T and B cell survival, dendritic cell fate, microglia
activation and endothelial cell signaling. These mechanisms, which could be usurped by
tumors to evade and thwart immune responses, have been proposed to shift the
balance of immune responses and control immune cell tolerance, inflammation and
angiogenesis.

"O-GlcNACYLATION : A NOVEL REGULATOR OF CELL SIGNALING AND CELL
CYCLE.
Dra. Vannesa Dehenaut. Institut de Biologie de Lille, Universit de Lille Nord de France

51

In response to mitogenic signals, quiescent cells (G0 arrested) enter and progress
through the four phases of the cell cycle: G1, S (DNA replication), G2 and M (Mitosis)
to produce two daughter cells genetically identical. Cell cycle is highly controlled by
numerous factors especially at the G1/S and G2/M boundaries to ensure that DNA is
not damaged and correctly replicated: loss of control is one of the major causes of
cancer.
For a few years, beside phosphorylation, O-GlcNAcylation has emerged as a new key
regulator of cell cycle. O-GlcNAcylation, standing for O-linked N-
acetylglucosaminylation is an abundant and reversible post-translational modification
confined within the nucleus and the cytosol of eukaryotes. This glycosylation, often
compared to phosphorylation with which it can compete, is regulated by a unique
couple of enzymes, the O-GlcNAc transferase (OGT) and the O-GlcNAcase (OGA)
which catalyse respectively the transfer and the hydrolysis of the sugar moiety.
First, our works have focused on the study of O-GlcNAcylation implication in the
control of Xenopus laevis oocyte meiotic resumption, a process analogous to the G2/M
transition of the cell cycle of somatic cells. This G2/M transition is characterized by the
simultaneous activation of the MPF (M-phase Promoting Factor), the universal
regulator of the M-Phase entry and of the MAPK Erk2 pathway. We demonstrated the
existence of a rise in oocyte O-GlcNAcylation at the G2/M transition [1] that was
essential to this process since inhibition of OGT prevented the activation of the MPF
and of the MAPK pathway [2]. Then we studied the variations of O-GlcNAcylation
occurring during the cell cycle of synchronised somatic cells and again we observed an
increase in O-GlcNAcylation at the G2/M transition whereas we demonstrated a
decrease of these glycosylation at the G1/S boundary correlated with an increased
OGA activity [3]. Moreover, we recently proved that serum stimulated cell cycle entry
promoted the synthesis of OGT and that the enzyme was necessary to the G0/G1
transition since its down-regulation by RNA interference prevented the activation of the

52
PI3K/Akt and of the MAP kinase pathways and led to a decrease in cyclin D1
expression [4].

By a combination of several approaches (proteomics, immunoprecipitation and Click-
chemistry), we were able to identify 24 functionally different O-GlcNAcylated proteins in
matured (M-phase) oocytes among which the proliferating cell nuclear antigen (PCNA)
and the MAPK Erk2 [5]. We also identified 58 proteins differentially O-GlcNAcylated at
the G1/S boundary including several components of the MCM 2-7 complex that take
parts in the regulation of DNA replication [3]. Finally, preliminary results showed that
cyclin D1, a central regulator of the G1 phase is O-GlcNACylated. Taken together our
results demonstrate that O-GlcNAcylation dynamic is essential for the good progress of
the cell cycle at all steps since it modifies master regulators of this process.

HUMAN SIGLEC-9 BINDING TO HYALURONIC ACID DAMPENS NEUTROPHIL
ACTIVATION AND IS SUBVERTED BY THE INVASIVE BACTERIAL PATHOGEN
GROUP A Streptococcus
Dr. Ismael SECUNDINO. University of California, San Diego, EUA

Sialic acid-binding Ig-Like Lectins (Siglecs) are immunoregulatory receptors on
leukocytes. A number of CD33-related human Siglecs (CD33rSiglecs) possess
intracellular inhibitory domains (ITIMs) that act to blunt activatory signaling
cascades. Siglec-9, abundantly expressed on human neutrophils, is known to engage
terminal sialic acid residues on cell surface glycoconjugates, to limit baseline neutrophil
activation. We previously described how the neonatal pathogen group B Streptococcus
(GBS) expresses a sialylated exopolysaccharide capsule that can engage Siglec-9 to
blunt neutron phil bactericidal responses and promote its own survival. Recently, in
screening the lectin repertoire of human Siglec-9, we made the unexpected discovery
that Siglec-9 strongly and specifically binds to high molecular weigh hyaluronic acid
(HMW-HA), through a region of its V-set domain distinct from the sialic acid-binding
domain. HA engagement by Siglec-9 limits neutrophil activation phenomenon including
oxidative burst, proinflammatory cytokine release and elaboration of DNA-based

53
extracellular traps (NETs), suggesting HA represents an important "self-associated
molecular pattern". The leading human pathogen group A Streptococcus (GAS)
possesses a capsule composed of HA indistinguishable from the host
polysaccharide. GAS HA is strongly upregulated in the shift of GAS from localized to
invasive bloodstream infection, and is known to promote bacterial resistance to
neutrophil and antimicrobial peptide killing. Using WT and isogenic GAS capsule-
deficient mutants, we show the pathogen can engage neutrophils and inhibit oxidative
burst and NET formation in a Siglec-9- and HA-dependent manner, promoting its own
survival. Thus, a single immunoregulatory lectin receptor on human neutrophils binds to
two different ubiquitous glycans and is subverted through molecular mimicry by two
different leading human pathogens.

54
NDICE DE AUTORES DE TRABAJOS
LIBRES POR TEMA

Biosntesis y metabolismo de glicoconjugados
[BM]
1. Acua Avila Pedro Estanislao BM-11
2. Alvarado-Vsquez Noe BM-05
3. Aquino Gil M. BM-17
4. Aquino Gil M. O. BM-09
5. Aragn Cuervas F. BM-18
6. Arenas-Del ngel MC. BM-15
7. Arenas-Ros Edith BM-04
8. Balderas-Anaya Madeline BM-16
9. Blanco-Labra A. BM-07
10. Calvillo Minerva BM-12
11. Campos-Guilln J. BM-07
12. Campos-Pea V. BM-02
13. Carbajal Aguilera K. BM-03
14. Castaeda Saucedo Eduardo BM-14

55
15. Castro Guilln JL. BM-07
16. Chavelas-Adame Eneas Alejandro BM-14
17. Chvez R. BM-15
18. Cisneros Solano A. BM-18
19. Corts Barberena Edith BM-04
20. Cruz Santiago J. BM-09
21. Cruz-Hernndez A. BM-07
22. Dehennaut V. BM-17
23. Della-Valle Daniela BM-12
24. Daz-Garca E.J. BM-10
25. Daz-Jimnez Diana Fabiola BM-01
26. Domnguez-Salazar E. BM-02
27. Espinosa Blanca BM-12
28. Ferriz-Martnez R. BM-07
29. Fierro Reyna BM-04
30. Flores-Carren Arturo BM-01
31. Foulquier F. BM-17
32. Garca-Gasca T. BM-07
33. Garca-Surez MD. BM-02
34. Garca-Surez MD. BM-03
35. Geiser-Dawn BM-08
36. Gmez-Olivares JL. BM-02

56
37. Gmez-Olivares JL. BM-03
38. Gonzlez-Hernndez Roberto de Jess BM-01
39. Guedri K. BM-17
40. Guevara Jorge BM-05
41. Guevara Jorge BM-12
42. Gutirrez-Ruiz MC. BM-03
43. Guzmn-Partida Ana BM-08
44. Hernndez Cruz P. BM-18
45. Hernndez-Chvez Marco Josu BM-01
46. Hernndez-Snchez Fernando BM-16
47. Illescas Barbosa D. BM-09
48. Isidro-Lzaro J. BM-10
49. Lagarda-Daz Irlanda BM-08
50. Lascurain R. BM-15
51. Lefebvre T. BM-17
52. Lefebvre Tony BM-05
53. Len-Galvn Miguel A. BM-04
54. Limon Daniel BM-05
55. Lpez Cortz Mara del Socorro BM-11
56. Lpez Durn RM. BM-02
57. Lpez-Durn RM. BM-03
58. Lpez-Prez Mercedes Guadalupe BM-06

57
59. Lozano Liliana BM-05
60. Martnez Cruz M. BM-18
61. Martnez-Alarcn D. BM-07
62. Martnez-Duncker Ivan BM-13
63. Martnez-Quezada R. BM-02
64. Matias Cervantes Carlos BM-18
65. Michalski J.C. BM-17
66. Mollicone Rosella BM-13
67. Morales Arroyo Ivis Ibrahum BM-03
68. Morales-Arroyo I. BM-02
69. Mora-Montes Hctor Manuel BM-01
70. Mora-Montes Hctor Manuel BM-06
71. Navarro-Arias Mara de Jess BM-06
72. Olivier-Van Stichelen S. BM-17
73. Patio Morales Carlos Csar BM-14
74. Prez-Campos E. BM-18
75. Prez-Cervera Y. BM-09
76. Perez-Cervera Y. BM-17
77. Prez-Santiago A.D. BM-10
78. Pina Canseco MS. BM-18
79. Pina-Canseco M.S. BM-10
80. Quezada-Martnez R. BM-03

58
81. Robles-Burgueo M. Refugio BM-08
82. Rodriguez Tobn Ahizer BM-04
83. Salinas Arreortua N. BM-02
84. Salinas Marin Roberta BM-13
85. Salinas-Arreortua N. BM-03
86. Snchez-Medina M.A. BM-10
87. Serrano H. BM-02
88. Serrano H. BM-03
89. Serrano Luna Armando Levith BM-14
90. Solrzano Mata C. J. BM-17
91. Solrzano-Mata C.J. BM-09
92. Tamez-Castrelln Alma Karina BM-01
93. Torres Martha BM-16
94. Trujillo-Esquivel Jos Elas BM-01
95. Vasquez Murrieta Mara Soledad BM-11
96. Vzquez-Moreno Luz BM-08
97. Vega-Hernndez Ricardo BM-16
98. Winzerling Joy BM-08
99. Zenteno E. BM-15
100. Zenteno Edgar BM-05
101. Zenteno Galindo E. BM-09
102. Zomosa Viviana BM-05

59
Desrdenes de la glicosilacin y
enfermedades raras [D]
103. Asteggiano CG. D-05
104. Asteggiano CG. D-06
105. Bistu Milln MB. D-05
106. Bolado Martnez Enrique D-04
107. Candia Plata Mara del Carmen D-04
108. Chacn S. D-05
109. D Grinberg D-06
110. De la Cruz Castillo Doris Yared D-01
111. Dodelson de Kremer R. D-05
112. EG Martnez Domenech D-06
113. Elso de Berberian G. D-05
114. G Matthijs D-06
115. Gallegos Velasco B. D-02
116. Gutirrez Candia Sergio D-04
117. Hernndez Cruz P. D-01
118. Hernndez Cruz P. D-02
119. Hernndez Cruz Pedro D-03
120. Hernndez Huerta Mara Teresa D-03
121. Lpez Soto Luis Fernando D-04

60
122. Lujn Camarillo Christian I. D-04
123. MA Delgado D-06
124. Martnez Cruz Margarito D-03
125. Martnez Cruz Ruth D-03
126. Mayoral Prez-Campos Laura D-03
127. N Guelbert D-06
128. P Sarrin D-06
129. Prez Campos E. D-01
130. Prez-Campos E. D-02
131. Prez-Campos Eduardo D-03
132. Pina-Canseco Socorro D-03
133. Pineda Orozco Dmaris D-04
134. R Dodelson de Kremer D-06
135. Rios Lpez Karen I. D-02
136. S Balcells D-06
137. Soto Guzmn Jess Adriana D-04
138. Spcola N. D-05
139. Syravegna M. D-05
140. Velasco Belm D-01
Glicobiologa de las enfermedades
infecciosas [EI]

61
141. Acosta-Blanco EI-05
142. Agis- Jurez Ral A. EI-03
143. Amyris Sandra EI-04
144. Barbosa Sabanero EI-06
145. Carmona Baca Karla EI-07
146. Cervantes-Landn A.Y. EI-01
147. Espinoza B. EI-01
148. Fernndez Gonzlez C.D. EI-09
149. Flores Moreno Karen EI-02
150. Flores Villavicencio L. EI-06
151. Fuentes-Romero EI-05
152. Garca-Ruz Viridiana EI-03
153. Gonzlez Canto A. EI-09
154. Gonzlez Garca C. Ramn EI-08
155. Gutirrez Huante Kathya EI-08
156. Huebner J. EI-02

62
157. Lpez Vancell R. EI-09
158. Lpez Vidal Y. EI-02
159. Lpez-Ramrez Luz A. EI-07
160. Lpez-Vidal Yolanda EI-03
161. Lozoya-Prez Nancy E. EI-07
162. Martnez Duncker R. Ivn EI-08
163. Martnez I. EI-01
164. Mndez Capdeville Teresita Mildred EI-10
165. Mora-Montes Hctor M. EI-07
166. Nequiz Avendao M. EI-09
167. Newburg EI-05
168. Ordua Patricia EI-03
169. Ortega Francisco EI-04
170. Ortega-Francisco EI-05
171. Perez Tamayo R. EI-09
172. Ramos Martnez E. EI-09

63
173. Rosas Alquicira Graciela EI-05
174. Ruiz-Palacios Y.J. EI-05
175. Sabanero Lpez Myrna EI-06
176. Servn-Gonzlez Luis EI-03
177. Solano Rodrguez Luciana EI-10
178. Soto Arredondo K. EI-06
179. Soto-Ramrez EI-05
180. Viveros-Rogel M. EI-05
Cncer [C]
181. Acevedo Prez Miguel ngel C-04
182. Aguilar-Lemarroy A. C-07
183. Almazo-Domnguez Roco C-06
184. Apresa-Garca T. C-07
185. Cant de Len D. C-05
186. Ceballos-Reyes Guillermo C-03
187. Ceja-Utrera F.J. C-07

64
188. Daz y Orea A. C-07
189. Encarnacin-Guevara S. C-05
190. Escobar-Snchez Ma. Luisa C-01
191. Fernndez-Herrera Mara Antonieta C-01
192. Flores-Mendoza Lilian C-06
193. Galicia-Morales Isaas C-01
194. Galindo Hernandez O. C-02
195. Gallardo-Rincn D. C-05
196. Gallegos Velasco B. C-04
197. Garca-Lpez Roco Berenice C-06
198. Garibay-Cerdenares OL. C-05
199. Gonzlez-Ballesteros Mauricio M. C-01
200. Gutirrez Iglesias Gisela C-03
201. Hernndez Cruz P. C-04
202. Hernndez-Ortz M. C-05
203. Hernndez-Pacheco R.E. C-07

65
204. Hernndez-Pacheco Raquel Esneidy C-06
205. Hernndez-Ramrez VI. C-05
206. Jave-Suarez L.F. C-07
207. Lpez-Muoz Hugo C-01
208. Mendieta-Carmona V. C-07
209. Njera Garca Nayelli C-03
210. Navarro Tito N. C-02
211. Osorio-Trujillo JC. C-05
212. Palma Lara Icela C-03
213. Prez Campos E. C-04
214. Prez Salazar E. C-02
215. Pina Canseco MS. C-04
216. Ramrez-Gutirrez Ramss Elas C-01
217. Reyes-Leyva J.R. C-07
218. Reyes-Leyva Julio C-06
219. Reyes-Salinas J.S. C-07

66
220. Rivera-Jurez M.A. C-07
221. Rodea-vila C. C-07
222. Rosas-Murrieta N.H. C-07
223. Rubio-Gayosso Ivan C-03
224. Snchez-Snchez Luis C-01
225. Sandoval-Ramrez Jess C-01
226. Santos-Lpez G. C-07
227. Santos-Lpez Gerardo C-06
228. Serna Marquez N. C-02
229. Sosa-Jurado F. C-07
230. Talams-Rohana P. C-05
231. Vallejo-Ruiz V. C-07
232. Vallejo-Ruiz Vernica C-06
233. Varela Castillo Omar C-03
234. Vargas-Maldonado M.T. C-07
235. Vsquez Aquino Marisol C-04

67
236. Vzquez-Zamora V.J. C-07
237. Villegas Comonfort S. C-02
238. Zamora-Guinez I. C-07
Glycobiotechnology [T]
239. Aguilera P. T-15
240. Aragn-Cuevas F. T-11
241. Arellano Crdenas S. T-06
242. Arellano Crdenas Sofa T-03
243. Bedoya-Lpez Andrea T-12
244. Bchs Jochen T-07
245. Cardos San Jorge F. T-01
246. Carranza-Rosales, P. T-10
247. Casabuono A. T-10
248. Chang Caldern J. T-01
249. Contreras-Esquivel J.C. T-08
250. Contreras-Esquivel J.C. T-10
251. Cornejo Mazn Maribel T-03
252. Cornejo Mazn Maribel T-05
253. Couto S.A. T-10
254. Cruz-Guerrero Alma T-04

68
255. Escamilla-Lozano Yolanda T-04
256. Espita C. T-14
257. Espitia Clara T-07
258. Estrada de los Santos P. T-08
259. Estrada Karel T-12
260. Farres A. T-15
261. Fernndez Santana V. T-01
262. Fragoso Castro Inari Idalith T-05
263. Galindo-Msico J.H. T-08
264. Gamboa-Suasnavart T-07
265. Garca Garibay Mariano T-04
266. Garca Rivero D. T-01
267. Garrido Arteaga R. T-01
268. Gavilondo Cowley J.V. T-13
269. Gomez-Ruiz Lorena T-04
270. Guilln D. T-14
271. Guilln D. T-15
272. Hernndez A. Vanessa T-09
273. Hernndez Fernndez M. A. T-06
274. Isidro-Lzaro J. T-02
275. Kloeckner Wolf T-07
276. Lpez Cortz M.S. T-06

69
277. Lpez Cortz Mara del Socorro T-05
278. Luna Ramrez Karem Yazmin T-03
279. Manzo Sandoval A. T-13
280. Marn-Palacio Luz D. T-07
281. Martnez Sotelo Jos A. T-07
282. Martnez-Cruz M. T-11
283. Medina Escutia M.E. T-13
284. Medina Flores Y. T-13
285. Moreno-Mendieta S. T-14
286. Moreno-Mendieta S. T-15
287. Morlett-Chvez J.A. T-10
288. Palomares Laura T-09
289. Pedroso Fernndez J. T-01
290. Prez-Santiago A.D. T-02
291. Pina-Canseco M.S. T-02
292. Ramrez Octavio T. T-09
293. Ramirez Octavio T. T-12
294. Ramn Gallegos E. T-13
295. Ramn-Delgado M.J. T-10
296. Rodrguez Noda L. M. T-01
297. Rodrguez-Sanoja, R. T-14
298. Rodrguez-Sanoja, R. T-15

70
299. Rodrguez-Serrano Gabriela T-04
300. Snchez S. T-15
301. Snchez-Esperanza F. T-11
302. Sanchez-Flores Alejandro T-12
303. Snchez-Medina M.A. T-02
304. Serrano-Rodrguez Y. T-01
305. Serratob Jos A. T-09
306. Servn-Gonzlez Luis T-07
307. Sosa-Ancona Erik T-04
308. Trujillo-Roldn Mauicio A. T-12
309. Trujillo-Roldn Mauricio A. T-07
310. Valds Balbn Y. T-01
311. Valdez-Cruz Norma A. T-07
312. Valdez-Cruz Norma A. T-12
313. Vrez Bencomo V. T-01
Inmunoglicobiologa [I]
314. Acevedo Prez Miguel ngel I-08
315. Agundias Mata C. I-03
316. Agundis Concepcin I-04
317. Alpuche Osorno J.J. I-03
318. Campa Angel I-04

71
319. Cancino Daz J. C. I-12
320. Cancino Daz M. E. I-12
321. Candia Plata Ma. Del Carmen I-07
322. Carvajal Dosamantes Armando I-07
323. Castro Leyva V. I-12
324. Cervantes Alfaro M. I-01
325. Eslava C. I-02
326. Espinosa Cueto P. I-09
327. Estrada Gutierrez G. I-12
328. Fenton Navarro B. I-01
329. Gallegos Velasco B. I-08
330. Galvn Moroyoqui Jos I-07
331. Garca Aguilar T.C. I-09
332. Garca Villa Denisse I-07
333. Garduo Ros D. I-01
334. Garfias Y. I-10
335. Gmez Chvez F. I-12
336. Gonzlez Christien Judith I-05
337. Hernndez Cruz P. I-08
338. Hernndez U I-02
339. Jaimes M. I-10
340. Jimnez-Martnez MC. I-10

72
341. Len L.A. I-02
342. Letechipa Vallejo G. I-01
343. Lpez Castillo Misael I-05
344. Lpez Soto Luis I-07
345. Lpez-Prez Mercedes Guadalupe I-11
346. Mancilla Jimnez R. I-09
347. Martnez Soto Juan I-07
348. Martnez-Duncker Ivn I-06
349. Martin-Manzo Miriam I-04
350. Meja H. I-10
351. Mora-Montes Hctor Manuel I-11
352. Navarro A. I-02
353. Navarro-Arias Mara de Jess I-11
354. Pereyra Ali I-04
355. Pereyra Morales A. I-03
356. Prez Campos E. I-08
357. Pina Canseco MS. I-08
358. Rodrguez Martnez S. I-12
359. Snchez Salgado J. L. I-03
360. Snchez-Salgado Jos Luis I-04
361. Santacruz C. I-10
362. Soto Gastelum Cecilia I-07

73
363. Soto Guzmn Adriana I-07
364. Torner Aguilar L. I-01
365. Villanueva Cabello Tania Mara I-06
366. Vivanco Rojas O. I-03
367. Vivanco-Rojas Oscar I-04
368. Zenteno E. I-03
369. Zenteno Edgar I-04
Presentaciones orales [O]
370. Albert Marina O-1
371. Alonso Daniel Fernando O-1
372. Arciniega-Fuentes M.T. O-4
373. Arteaga-Cabello F.J. O-4
374. Cardoso San Jorge, F. O-3
375. Caretti Anna O-5
376. Chang Caldern J. O-2
377. Chang Caldern, J. O-3
378. Collin M. O-10
379. DallOlio Fabio O-5
380. Dehnnaut V. O-6
381. Escobar-Ramirez Adelma O-6
382. Espitia-Pinzn C. O-10

74
383. Estrada I. O-10
384. Fernndez Santana V. O-2
385. Fernndez Santana V. O-3
386. Fernndez Villalobos A. O-2
387. Ferreira IC. O-9
388. Freire-de-Lima L. O-8
389. Gabri Mariano Rolando O-1
390. Garca-Rivera D. O-3
391. Garrido Arteaga R. O-2
392. Garrido Arteaga, R. O-3
393. Gavilondo Cowley J.V. O-7
394. Gomez Daniel Eduardo O-1
395. Hernandez-Pando R. O-10
396. Hoedt Esthelle O-6
397. Huvent Isabelle O-6
398. Manzo Sandoval A. O-7
399. Marquina B. O-10
400. Mata- Espinoasa D. O-10
401. Medina Escutia M.E. O-7
402. Medina Flores Y. O-7
403. Mendona-Previato L. O-8
404. Mendona-Previato L. O-9

75
405. Moreno Paredes B. O-3
406. Newburg D.S. O-4
407. Olivares- Arzuaga N. O-10
408. Pedroso Fernndez J. O-2
409. Pierce Annick O-6
410. Previato JO. O-8
411. Ramirez Gonzlez U. O-2
412. Ramn Gallegos E. O-7
413. Rodrguez Noda L. O-2
414. Rodrguez Noda, L. O-3
415. Rook G. O-10
416. Ruiz-Palacios G.M O-4
417. S-Diniz JN O-8
418. Santana Fernndez D. O-2
419. Santana Medero D. O-3
420. Segatori Valeria Ins O-1
421. Signorelli Paola O-5
422. Takiya CM O-8
423. Teixeira PAC. O-9
424. Trinchera Marco O-5
425. Valds Balbn Y. O-2
426. Valds Balbn, Y. O-3

76
427. Valente RC O-8
428. Vrez Bencomo V. O-3
429. Vrez Bencomo V. O-2
430. Villar Aneiros A. O-2
431. Villar Aneiros, A. O-3
432. Zatarain-Barron ZL. O-10
433. Zulueta Aida O-5

77
RELACION DE TRABAJOS LIBRES

BIOSNTESIS Y METABOLISMO DE
GLICOCONJUGADOS
BM-01 FUNCIONAL ANALISIS OF Candida albicans MNN4-LIKE GENE
FAMILY. Tamez-Castrelln Alma Karina, Gonzlez-Hernndez
Roberto de Jess, Flores-Carren Arturo, Hernndez-Chvez Marco
Josu, Trujillo-Esquivel Jos Elas, Daz-Jimnez Diana Fabiola and
Mora-Montes Hctor Manuel*.
BM-02 GLYCOSYLATION OF CHOLINESTERASES IN HIPPOCAMPUS FROM
RATS TREATED WITH AF64A Martnez-Quezada R
1
, Morales-Arroyo
I, Lpez-Durn RM
1
, Garca-Surez MD
2
, Salinas-Arreortua N
1
,
Serrano H, Domnguez-Salazar E, Campos-Pea V, Gmez-Olivares
JL.
BM-03 ANALISIS OF THE GLYCOSYLATION IN CHOLINESTERASES EXTRACTED
OF NONALCOHOLIC FATTY LIVER FROM MOUSE TREATED WITH
HYPERCHOLESTEROLEMIC DIET. Ivis Ibrahim Morales Arroyo ,
Quezada-Martnez R1*,Lpez-Durn RM1, Garca-Surez MD2,
Salinas-Arreortua N1, Carbajal-Aguilera K3, Serrano H1, Gutirrez-
Ruiz MC1, Gmez-Olivares JL1
BM-O4 DYNAMICS OF THE CARBOHYDRATES ON SPERM PLASMA
MEMBRANE OF CORYNORHINUS MEXICANUS BAT AS A SPERM
MATURATION MODEL. Rodrguez-Tobn Ahiezer, Fierro Reyna,
Corts Barberena Edith, Len-Galvn Miguel A. and Arenas-Ros
Edith
BM-05 EFFECT OF -AMYLOID 25-35 PEPTIDE ON THE O-GlcNAcylation AND
PHOSPHORYLATION Tau PROTEIN IN HYPERGLICEMIC RATS. Liliana
Lozano, Noe Alvarado-Vsquez, Viviana Zomosa , Daniel Limon ,
Tony Lefebvre , Edgar Zenteno, and Jorge Guevara.

78
BM-06 RELEVANCE OF PROTEIN GLYCOSYLATION PATHWAYS FOR IMMUNE
SENSING OF Candida guilliermondii. Navarro-Arias Mara de Jess
1
,
Lpez-Prez Mercedes Guadalupe, Mora-Montes Hctor Manuel.
BM-07 MODELING A TEPARY BEAN (Phaseolus acutifolius) LECTIN FROM
THE DEDUCED POLYPEPTIDE SEQUENCE OF A mRNA EXPRESSED IN
E. coli. Martnez-Alarcn D, Castro-Guilln JL, Cruz-Hernndez A,
Campos-Guilln J, Ferriz-Martnez R, Blanco-Labra A, Garca-Gasca
T.
BM-08 GLICOPROTEOMICS OF THE MIDGUT LARVAL OF Zabrotes
subfasciatus ASSOCIATED TO THE INSECTICIDAL MECHANISM OF
PF2 LECTIN FROM Olneya tesota. Lagarda-Daz Irlanda, Guzmn-
Partida Ana , Robles-Burgeo M. Refugio, Geiser Dawn, Winzerling
Joy and Vzquez-Moreno Luz
BM-09 O-GLYCOSYLATION EXPRESSION IN CERVICAL CANCER CELL LINES BY
AMARANTHUS LEUCOCARPUS LECTIN. Illescas Barbosa D. Cruz
Santiago J. Aquino Gil M. O. Prez-Cervera Y. Zenteno Galindo E.
Solrzano-Mata C. J.
BM-10 INTERACTION OF PLANT LECTINS WITH Aspergillus parasiticus. Daz-
Garca E.J., Isidro-Lzaro J., Pina-Canseco M.S, Snchez-Medina
M.A, Prez-Santiago A.D.
BM-11 CONTENT OF PICEID FROM MEXICAN GRAPE AND WINE (CABERNET
SAUVIGNON). Acua Avila Pedro Estanislao, Lopz Cortz Ma. del
Socorro ,Vasquez Murrieta Mara Soledad .
BM-12 INDUCTION OF GLYCOSYLATION AND MORPHOLOGICAL
DIFFERENTIATION BY DEXAMETHASONE AND ALL-TRANS RETINAL
IN C6 CELLS. Della-Valle Daniela, Espinosa Blanca, Calvillo
Minerva , Guevara Jorge

79
BM-13 A FUNCTIONAL SPLICING VARIANT OF CMP-SIALIC ACID
TRANSPORTER. Roberta Salinas-Marin,

Rosella Mollicone,

Ivan
Martinez-Duncker
BM-14 RECOGNITION AND DIFFERENTIAL AGLUTINATION OF CERVICAL
CANCER CELLS WITH A NOVEL LECTIN FROM TUNA SHELL. Patio
Morales Carlos Csar 1, Serrano Luna Armando Levith
1
, Chavelas-
Adame Eneas Alejandro
1
, Castaeda Saucedo Eduardo
2
BM-15 COESTIMULACIN DE LINFOCITOS T CD4+ MURINOS MEDIADA POR
N-ACETIL GALACTOSAMINA. Arenas-Del ngel MC, Chvez R,
Zenteno E, Lascurain R
BM-16 THE VITAMIN D RECEPTOR IS TARGET OF O-GLcNAc
GLYCOSYLATION UNDER HYPERGLYCEMIC CONDITIONS IN THP1-
CELLS. Hernndez-Snchez Fernando
1
, Balderas-Anaya Madeline
1
.
Vega-Hernndez Ricardo
1
and Torres Martha
1
.
BM-17
COMPARATIVE STUDY OF GENE EXPRESSION OF GLU1 AND
GLU2 OF BETA-GLUCOSIDASE IN TEOSINTE Z. parviglumis,
Z. diploperennis, Z. mexicana and Z. luxurians

DESRDENES DE LA GLICOSILACIN Y
ENFERMEDADES RARAS
D-01 EXPRESSIN OF ANTIGEN T AND GALECTIN-3 IN TESTIS APPENDIX IN
CHILDREN WITH CRYPTORCHIDISM. De la Cruz Castillo Doris
Yared,Velasco Belm1, Prez Campos E2 y Hernandez Cruz P
D-02 EXPRESSIN OF MUCINS 1, 2, 5, TA998, DF3 AND GALECTIN-3 IN
TESTIS APPENDIX IN CHILDREN WITH CRYPTORCHIDISM. Rios
Lpez Karen I, Gallegos Velasco B, Prez-Campos E y Hernndez
Cruz P.

80
D-03 RECOGNITION OF TRITRICUM VULGARIS LECTIN IN PLATELETS
MEMBRANES OF SUBJECTS RESISTANT INSULIN. Hernndez
Huerta Mara Teresa, Prez-Campos Eduardo, Hernndez Cruz
Pedro , Mayoral Prez-Campos Laura, Martnez Cruz Margarito,
Pina-Canseco Socorro, Martnez Cruz Ruth

D-04 Terminal glycosylation of immunoglobulin G from type 2 diabetes
mellitus patients". Candia Plata Maria del Carmen, Pineda Orozco
Dmaris Lujn Camarillo Christian I. , Bolado Martnez Enrique ,
Lpez Soto Luis Fernando , Gutirrez Candia Sergio , Soto Guzmn
Jess Adriana
D-05 NCX1 AND NCKX1 CA+2 EXCHANGERS IN HUMAN PLATELETS: A
PUTATIVE ROLE IN THE THROMBUS-HEMORRHAGIC EVENTS
ASSOCIATED WITH CONGENITAL DISORDER OF GLYCOSYLATION.
Bistu Milln MB; Syravegna M; Spcola N, Chacn S; Dodelson de
Kremer R; Elso de Berberian G; Asteggiano CG
D-06 SKELETAL DYSPLASIA DUE TO CONGENITAL DISORDERS OF
GLYCOSYLATION. EG Martinez Domenech; MA Delgado; P Sarrin
2
;
G Matthijs
3
; N Guelbert; R Dodelson de Kremer; S Balcells; D
Grinberg; CG Asteggiano.

GLICOBIOLOGA DE LAS ENFERMEDADES
INFECCIOSAS
EI-01 SEROLOGICAL TESTS IMPROVEMENT FOR CHAGAS DISEASE
DIAGNOSIS. Cervantes-Landn A.Y., Martnez I., Espinoza B.
EI-02 CHARACTERIZATION OF THE ADAPTIVE IMMUNE RESPONSE IN E.
FAECIUM" Karen Flores Moreno,Huebner J., Lpez Vidal Y.
EI-03 DIFFERENCES IN THE GLYCOSYLATION PROFILE OF MYCOBACTERIUM
MICROTI RECOMBINANT STRAINS. Viridiana Garca-Ruz , Patricia
Ordua , Luis Servn-Gonzlez , Ral A. Agis- Jurez , and Yolanda
Lpez-Vidal

81
EI-04 MODULATION OF THE SURFACE GLYCOSILATION IN HUMAN
MONOCYTES DURING DENGUE VIRUS INFECTION " Sandra Amyris
Ortega Francisco
EI-05 HUMAN MILK GLYCOCONJUGATES INHIBIT HIV-1 LABORATORY
STRAINS AND PRIMARY ISOLATES IN VITRO" Graciela Rosas
Alquicira,, Acosta-Blanco , Ortega-Francisco, Fuentes-Romero,
Newburg, Ruiz-Palacios Y, J, Soto-Ramrez, Viveros-Rogel M
EI-06 GLYCOPROTEINS FROM A castellanii AND ESTABLISHMENT OF
INFECTION IN NEURONAL CELLS" Myrna Sabanero Lpez ,Soto
Arredondo K.1, Flores Villavicencio L, Barbosa Sabanero
EI-07 ANALYSIS OF THE OCH1 GENE FAMILY IN Sporothrix schenckii" Karla
Carmona Baca, Lozoya-Prez Nancy E., Lpez-Ramrez Luz A.,
Mora-Montes Hctor M
EI-08 ADENOVIRUS HUMANO AD5 MODIFICA LA FUCOSILACIN CELULAR
EN UN MODELO IN VITRO DE EPITELIO PULMONAR HUMANO.
Gutirrez Huante Kathya , Gonzlez Garca C. Ramn , Martnez
Duncker R. Ivn .
EI-09 A MANNOSE RICH GLYCOCONJUGATE PRESENT IN trophozoites from
E. histolytica JUST recovered from experimental liver abscesses.
Fernndez Gonzlez C.D., Nequiz Avendao M., Gonzlez Canto A.,
Ramos Martnez E., Lpez Vancell R. & Perez Tamayo R.
EI-10 SEQUENCE SIMILARITY OF THE ENZYMES INVOLVED IN THE
BIOSYNTHESIS OF CAPSULAR POLYSACCHARIDES IN TWO MEDICAL
IMPORTANT BACTERIA AS POSSIBLE RESPONSIBLE FOR THE
PRESENCE OF CROSSED IMMUNITY. Solano Rodrguez
Luciana ,Mndez Capdeville Teresita Mildred .

GLICOBIOLOGA DEL CNCER

82
C-01 SELECTIVE ANTICANCER ACTIVITY OF SPIROSTANIC
GLYCOCONJUGATES Fernndez-Herrera Mara Antonieta,
Sandoval-Ramrez Jess, Snchez-Snchez Luis, Ramrez-Gutirrez
Ramss Elas, Galicia-Morales Isaas, Escobar-Snchez Ma. Luisa,
Gonzlez-Ballesteros Mauricio M., Lpez-Muoz Hugo.
C-02 ARACHIDONIC ACID INDUCES AN INCREASE OF -1,4-
GALACTOSYLTRANSFERASE I EXPRESSION IN MDA-MB-231 BREAST
CANCER CELLS. Villegas Comonfort S, Navarro Tito N, Serna
Marquez N, Galindo Hernandez O, Prez Salazar E.

C-03 STUDY OF LECTIN BINDING PATTERN IN LUNG CANCER CELLS
TREATED WITH (-)-EPICATECHIN. Njera Garca Nayelli, Varela
Castillo Omar, Rubio-Gayosso Ivan, Palma Lara Icela, Gutirrez
Iglesias Gisela, Ceballos-Reyes Guillermo.
C-04 EXPRESSION OF SIALIC ACID AND GALECTIN-3 IN MCF-7 CELLS
STIMULATED WITH TNF . Acevedo Prez Miguel ngel Vsquez
Aquino Marisol, Hernndez Cruz P, Prez Campos E, Pina Canseco
MS y Gallegos Velasco B.
C-05 PROTEOMIC IDENTIFICATION OF DIFFERENT PROFILES OF
FUCOSYLATED HAPTOGLOBIN ISOFORMS IN ASCITIC FLUID FROM
OVARIAN CYSTADENOCARCINOMA MEXICAN PATIENTS.
CORRELATION WITH ADVANCED CLINICAL STAGES. Garibay-
Cerdenares OL
1
, Osorio-Trujillo JC
1
, Hernndez-Ramrez VI
1
,
Hernndez-Ortz M
3
, Gallardo-Rincn D
2
, Cant de Len D
2
,
Encarnacin-Guevara S
3
and Talams-Rohana P
1
.
C-06 TUMOR ANTIGEN SLE (X) EXPRESSION IN CERVICAL SCRAPES WITH
RECURRING SQUAMOUS INTRAEPITHELIAL LESION. Hernndez-
Pacheco Raquel Esneidy
1
, Garca-Lpez Roco Berenice, Almazo-
Domnguez Roco
2
, Flores-Mendoza Lilian
1
, Santos-Lpez Gerardo
1
,
Reyes-Leyva Julio
1
, Vallejo-Ruiz Vernica
1
. Centro de Investigacin
Biomdica de Oriente, IMSS
1
Hospital General de Zona No. 5
Metepec
2

83
C-07 POLIMORFISMOS DE UN SOLO NUCLETIDO EN EL PROMOTOR B3
DEL GEN ST3GAL 4 EN PACIENTES CON LESIN ESCAMOSA
INTRAEPITELIAL CERVICAL Y CNCER RVICOUTERINO. a-Jurez
M.A.1,2, Rosas-Murrieta N.H.2, Mendieta-Carmona V.1,
Hernndez-Pacheco R.E.4, Rodea-vila C.3, Apresa-Garca T.3,
Jave-Suarez L.F.5, Aguilar-Lemarroy A.5, Ceja-Utrera F.J.6,
Vzquez-Zamora V.J.6, Reyes-Salinas J.S.6, Vargas-Maldonado
M.T.6, Daz y Orea A.4, Zamora-Guinez I.4, Sosa-Jurado F.1, Santos-
Lpez G.1, Reyes-Leyva J.R.1, and Vallejo-Ruiz V.1.

GLICOBIOTECNOLOGA
T-01 RELEVANCE OF O-ACETYL AND PHOSPHOGLYCEROL GROUPS FOR
THE ANTIGENICITY OF STREPTOCOCCUS PNEUMONIAE SEROTYPE
18C CAPSULAR POLYSACCHARIDE.
Chang Caldern J.; Serrano Rodrguez Y.; Garrido Artega R.;
Rodrguez Noda L. M.; Pedroso Fernndez J.; Cardoso San Jorge F.;
Valds Balbn Y.; Garca Rivero D.; Fernndez Santana V.*; Vrez
Bencomo V.

T-02 LECTIN OF CORN COLEOPTILE BEHAVIOR AS A RESPONSE AGAINST
Aspergillusparasiticus in vivo. Snchez-Medina M.A., Isidro-Lzaro
J., Pina-Canseco M.S., Prez-Santiago A.D.
T-03 ANTHOCYANIN CONTENT, PHENOLIC COMPOUNDS AND
ANTIOXIDANT ACTIVITY IN BLACKBERRY FRUIT (RUBUS
FRUCTICOSUS) AND JAM FROM ESTADO DE MXICO. Luna
Ramrez, Karem Yazmin.; Arellano Crdenas, Sofa.; Cornejo
Mazn, Maribel.
T-04 PRODUCTION AND PARCIAL PURIFICATION of fucosyl-
oligosaccharides by transgalactosilation reaction. Escamilla-Lozano
Yolanda, Sosa-Ancona Erik, Garca-Garibay Mariano, Gomez-Ruiz
Lorena, Rodrguez-Serrano Gabriela, Cruz-Guerrero Alma

84
T-05 CHARACTERIZATION AND DETERMINATION OF PHENOLIC
COMPOUNDS, ANTHOCYANIN CONTENT AND REDUCING SUGARS
PRESENT IN MINOR FRUITS (BLUEBERRY, RASPBERRY,
STRAWBERRY AND BLACKBERRY) HARVESTED IN MEXICO. Fragoso
Castro Inari Idalith, Lpez Cortz, Ma. del Socorro, Cornejo Mazn,
Maribel
T-06 PHYSICOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT
PROPERTIES OF GRAPEFRUIT JUICE (CITRUS PARADISI) Hernndez
Fernndez M. A. Arellano Crdenas S. . Lpez Cortz M. S.
T-07 STRATEGIES TO PRODUCE RECOMBINANT GLYCOPROTEINS IN
FILAMENTOUS BACTERIA: FROM SHAKE FLASK TO BIOREACTOR IN
THE PRODUCTION OF APA FROM MYCOBACTERIUM
TUBERCULOSIS IN STREPTOMYCES LIVIDANS.Ramss A. Gamboa-
Suasnavart , Luz D. Marn-Palacio , Wolf Kloeckner , Jos A.
Martnez-Sotelo , Clara Espitia , Luis Servn-Gonzlez , Norma A.
Valdez-Cruz , Jochen Bchs and Mauricio A. Trujillo-Roldn
T-08 INDUSTRIAL GLYCOBIOLOGY OF Burkholderia tropica AS SELECTED
STRAIN FOR CARBOHYDRATE MODIFICATION. Galindo-Msico J.H,
Contreras-Esquivel J.C, Estrada de los Santos P.
T-09 NP-HPLC GLYCOSYLATION ANALYSIS OF RECOMBINANT PROTEINS
PRODUCED BY ANIMAL CELL CULTURE. Vanessa Hernndez A, Jos
A. Serratob, Octavio T. Ramreza and Laura Palomares
T-10 OBTAINMENT A NUTRACEUTICAL OF MARINE ORIGIN BY
MICROWAVE-ASSISTED GREEN TECHNOLOGY" Ramn Delgado
Mara de Jess
T-11 IDENTIFICATION OF MOLECULAR COMPLEX (CCL- -GLUCOSIDASE)
FROM NATIVES MAIZE OF OAXACA STATE.Snchez-Esperanza F,
Aragn-Cuevas. F, Martnez-Cruz M.

85
T-12 EFFECT OF MILD HYPOTHERMIA ON SECRETION AND QUALITY OF
RECOMBINANT GLYCOPROTEIN tPA IN CHO CELLS. Bedoya-Lpez
Andrea 1, Estrada Karel 2,Sanchez-Flores Alejandro 2, Ramrez
Octavio T 3, Trujillo-Roldn Mauricio A.1, Valdez-Cruz Norma A.
T-13
DEVELOPMENT OF SINGLE CHAIN FV ANTIBODY FRAGMENT
AGAINST -GAL EPITOPE WITH POTENCIAL USE FOR IN SITU
DIAGNOSIS OF HUMAN PAPILLOMAVIRUS. Manzo Sandoval A. 1,
Gavilondo Cowley J.V. 2, Medina Escutia M.E. 3, Medina Flores Y. 3,
Ramn Gallegos E.
T-14
STARCH AS DELIVERY SYSTEM FOR ORAL VACCINATION. Guilln D., Moreno-Mendieta S.,
Espita, C., Rodrguez-Sanoja, R.

T-15
THE CARBOHYDRTE BINDING CAPACITY AS A TOOL FOR THE
PURIFICATION OF RECOMBINANT PROTEINS. Rodrguez-Sanoja, R.
1
,
Guilln D.
1
, Aguilera, P.
1
, Moreno-Mendieta S.
1
, Snchez S.
1
, Farres A.
2

INMUNOGLICOBIOLOGA
I-01 CITOKINES AND GALECTIN-3 IN ACUTE GLOBAL CEREBRAL ISCHEMIA
IN RATS TREATED WITH MELATONIN" . Garduo Ros D, Torner
Aguilar L, Cervantes Alfaro M, Letechipa Vallejo G and Fenton
Navarro B.
I-02 PEPTIDE MIMOTOPES SELECTED BY PHAGE DISPLAY FROM A REGION
OF E. coli O157 LPS INDUCE ANTIBODY EXPRESSION AGAINST O157
LPS" Navarro A, Hernndez U, Len L A, Licona D, Eslava C
I-03 Identification of hemocytes lectin homoreceptor of the crayfish
Cherax quadricarinatus" Snchez Salgado J. L., Pereyra Morales A,
Alpuche Osorno J. J, Vivanco Rojas O, Agundis Mata C, Zenteno E

86
I-04 A PARTIAL CHARACTERIZATION OF A SERIC LECTIN OF THE SHRIMP
LITOPENAEUS VANAMEII" Vivanco-Rojas Oscar , Martin-Manzo
Miriam , Campa Angel , Pereyra Ali , Snchez-Salgado Jos Luis ,
Zenteno Edgar , Agundis Concepcin
I-05 INCREASED SIALYLATION ON MACROPHAGES BY LPS TREATMENT".
Lpez Castillo Misael, Gonzlez Christen Judith
I-06 ACTIVATION OF NAVE CD4+ T CELLS IS ASSOCIATED TO
HYPERSIALYLATION AT THE EXPENSE OF GANGLIOSIDE SYNTHESIS
" Tania Mara Villanueva Cabello, Martnez-Duncker Ivn
I-07 FERRITIN GLYCATED AND GLICOXIDATED INCREASED EXPRESSION
AND ACTIVATION OF TOLL-LIKE RECEPTORS 2 AND 4 IN HUMAN
CD14 CELLS." Galvn Moroyoqui Jos , Candia Plata Ma. del
Carmen , Martnez Soto Juan, Soto Guzmn Adriana , , Garca Villa
Denisse , Carvajal Dosamantes Armando, Soto Gastelum Cecilia ,
Lpez Soto Luis
I-08 EXPRESSION OF GALECTIN-3 AND ANTIGEN T IN PLATELETS. Acevedo
Prez Miguel ngel
1
, Gallegos Velasco B
1
, Prez Campos E
1
, Pina
Canseco MS
1
y Hernndez Cruz P*
1
.
I-09 PARTICIPATION OF CARBOHYDRATES IN MYCOBACTERIAL
PHAGOCYTOSIS OF APOPTOTIC BODIES." Garca Aguilar T.C.,
Espinosa Cueto P., Mancilla Jimnez R.
I-10 MUC2 AND TNF-ARE INCREASED IN TEARS OF PATIENTS WITH
ADENOVIRUS OCULAR INFECTIO. Santacruz C , Jaimes M , Meja H ,
Garfias Y , Jimnez-Martnez MC
I-11 RELEVANCE OF PROTEIN GLYCOSYLATION PATHWAYS FOR IMMUNE
SENSING OF Candida guilliermondii. "Navarro-Arias Mara de Jess,
Lpez-Prez Mercedes Guadalupe, Mora-Montes Hctor Manuel

87
I-12 INHIBITORY EFFECT OF GALECTIN-1 OVER IL-6 EXPRESSION IN LPS-
TREATED DECIDUAL CELLS BY REDUCING IB. Gmez Chvez F 1,
Castro Leyva V 2, Estrada Gutierrez G 2, Cancino Daz J C 1, Cancino
Daz M E 1, Rodrguez Martnez S 1.

TRABAJOS ORALES
O-01 INDUCED EXPRESSION OF N-GLYCOLYLNEURAMINIC ACID IN B16
MELANOMA CELLS, MODULATES CAVEOLIN-1 AND INTEGRIN a5
EXPRESSION AND PROMOTES MESENCHYMAL-LIKE MORPHOLOGY.
Segatori Valeria Ins, Albert Marina, Gomez Daniel Eduardo,
Alonso Daniel Fernando, Gabri Mariano Rolando.
0-02 PROCEDURE TO OBTAIN IMMUNOGENIC CONJUGATES FROM THE CAPSULAR
POLYSACCHARIDES OF SEROTYPES 5, 14 AND 18C OF STREPTOCOCCUS
PNEUMONIAE TO TETANUS TOXOID.
Villar Aneiros A., Ramirez Gonzlez U., Chang Caldern J., Pedroso Fernndez J.,
Fernndez Villalobos A., Garrido Arteaga R., Rodrguez Noda L., Santana
Fernndez D., Valds Balbn Y. Fernndez Santana V.*, Vrez Bencomo V.
0-03 AN HEPTAVALENT CONJUGATE VACCINE AGAINST THE LATINOAMERICAN
MOST PREVALENT SEROTYPES OF STREPTOCOCCUS PNEUMONIAE"Valds
Balbn, Y.; Santana Medero, D.; Garca-Rivera, D.; Moreno Paredes, B.; Chang
Caldern, J.; Cardoso San Jorge, F.; Garrido Arteaga, R.; Villar Aneiros, A. ;
Rodrguez Noda, L.; Fernndez Santana, V.;Vrez Bencomo, V.
0-04 USE OF fkp GENE FROM Bacteroides fragilis TO BOOST THE SYNTHESIS OF
FUCOSYLATED OLIGOSACCHARIDES IN E. coli. Arteaga-Cabello F.J, Arciniega-
Fuentes M.T. Newburg D.S., Ruiz-Palacios G.M

88
0-05 TRANSCRIPTIONAL REGULATION OF b1,3 GALACTOSYLTRANSFERASE b3Gal-T5:
ROLE OF HEPATOCYTE NUCLEAR FACTOR HNF1. Zulueta Aida, Caretti Anna,
Signorelli Paola, DallOlio Fabio,Trinchera Marco.
0-06 A CROSSTALK BETWEEN O-GLCNACYLATION, PHOSPHORYLATION,
UBIQUITINATION AND SUMOYLATION CONTROLS THE TRANSCRIPTIONAL
ACTIVITY OF DELTA-LACTOFERRIN. Escobar-Ramirez Adelma, Huvent Isabelle,
Hoedt Esthelle, Dehnnaut V and Pierce Annick
0-07 DEVELOPMENT OF SINGLE CHAIN FV ANTIBODY FRAGMENT AGAINST -GAL
EPITOPE WITH POTENCIAL USE FOR IN SITU DIAGNOSIS OF HUMAN
PAPILLOMAVIRUS. Manzo Sandoval A. , Gavilondo Cowley J.V. , Medina
Escutia M.E. , Medina Flores Y. , Ramn Gallegos E.
0-08 POSSIBLE INVOLVEMENT OF TUMOR-ASSOCIATED LEUKOSIALIN CD43 IN
EPITHELIAL-MESENCHYMAL TRANSITION" S-Diniz JN, Valente RC, Takiya CM,
Mendona-Previato L, Previato JO, Freire-de-Lima L.
0-09 Cryptococcus neoformans MANNOPROTEINS ADHESIVE PROPERTIES. Teixeira
PAC, Mendona-Previato L, Ferreira IC and Previato JO.
0-10 THE THERAPEUTIC EFFECT OF IMMUNOGLOBULIN IN MURINE TUBERCULOSIS
IS DEPENDENT ON IgG GLYCOSYLATION. Olivares- Arzuaga N , Marquina B ,
Mata- Espinoasa D , Zatarain-Barron ZL , Espitia-Pinzn C , Estrada I, Collin M ,
Rook G and Hernandez-Pando R

89

RESMENES DE TRABAJOS LIBRES

BM-01 FUNCTIONAL ANALISIS OF Candida albicans MNN4-LIKE GENE FAMILY.
Tamez-Castrelln Alma Karina, Gonzlez-Hernndez Roberto de Jess, Flores-Carren Arturo,
Hernndez-Chvez Marco Josu, Trujillo-Esquivel Jos Elas, Daz-Jimnez Diana Fabiola and Mora-
Montes Hctor Manuel*. Departamento Biologa, Divisin de Ciencias Naturales y Exactas, Campus
Guanajuato, Universidad de Guanajuato. Divisin de Ciencias Naturales y Exactas, Campus Guanajuato,
Universidad de Guanajuato, Noria Alta s/n, Col. Noria Alta, C.P. 36050, Guanajuato, Gto., Mxico. *
Corresponding author: tel 473 732 0006, ext. 8154; email: hmora@ugto.mx

Candida albicans is an opportunistic fungal pathogen that causes infections in the
human being. Adhesion to host cells is a key process during tissue invasion, and this is
achieved by cell wall adhesins. In addition, the cell wall is a source of several virulence
factors and antigens recognised by the hosts defense mechanisms. The C. albicans
-glucans, and an external layer rich
in mannoproteins. These proteins are modified by mannose-rich oligosaccharides,
which contain mannose residues linked to the oligosaccharide core by phosphodiester
bounds, and thus are named phosphomannan. These structures are relevant during
phagocytosis of the fungal cells by macrophages and for the action of cationic
antimicrobial peptides. The biosynthesis of phosphomannans has been characterized in
Saccharomyces cerevisiae, where the Ktr6 protein is the main
mannosylphosphotransferase and Mnn4 is a positive regulator for enzyme activity.
However, the pathway is different in C. albicans, and so far MNT3 and MNT5 have been
characterized as genes encoding for enzymes that contribute up to 50% of the total
mannosylphosphotransferase activity. The MNN4-like family has seven members with
BIOSNTESIS Y METABOLISMOS DE GLICOCONJUGADOS
(BM)

90
significant similarity to the positive factor MNN4, and therefore, it is likely at least one of
its members participate in the biosynthesis of phosphomannan. In this work, using
deletion and overexpression approaches, we demonstrated that Mnn41, Mnn42, Mnn43
and Mnn44 participate in the synthesis of phosphomannans in C. albicans, as proteins
with both functions mannosylphosphotransferase activity and positive regulators.


BM-02 GLYCOSYLATION OF CHOLINESTERASES IN HIPPOCAMPUS FROM RATS
TREATED WITH AF64A
Martnez-Quezada R
1*
, Morales-Arroyo I
1*
, Lpez-Durn RM
1
, Garca-Surez MD
2
, Salinas-Arreortua N
1
,
Serrano H
1
, Domnguez-Salazar E
3
, Campos-Pea V
4
, Gmez-Olivares JL
1
. Departamentos de Ciencias
de la Salud
1
, Biologa
2
, Biologa de la Reproduccin
3
. Posgrado en Biologa Experimental*. Universidad
Autnoma Metropolitana-Iztapalapa. Instituto Nacional de Neurologa y Neurociruga
4
. Mxico.
Av. San Rafael Atlixco, nmero 186. Colonia: Vicentina. CP. 09340: Iztapalapa. Mxico
rebemarq_710@hotmail.com

One possible explanation for the deficiencies in learning skills, memory and behavioral
alterations associated to the continuous decline in dementia is known as the cholinergic
hypothesis. According to this, alterations are caused, at least in part, by decreased
levels of acetylcholine in the brain, as has been induced by AF64A administration.
However, changes in the processing of cholinesterases (ChEs) are unknown. ChEs are
glycoproteins, whose sugar content are 10-15% from their molecular weight. In the rat,
acetylcholinesterase has three potential glycosylation sites, whereas up to eight sites
can be predicted for butyrylcholinesterase. The glycosylation process of proteins is
regulated by specific glycosydases and glycosyltransferases. Carbohydrate
incorporation is important for the tridimensional structure in cholinesterases. In this
work, we estimated the acetyl- and butyrylcholinesterase activities and analyzed the
glycosylation of cholinesterases in the CA1 hippocampal region from rat lesioned with
AF64A. Results showed differences in percentages interaction with lectins between

91
extracted cholinesterases from control rats and rats administrated with AF64A. These
data indicate that a damage brain may alter protein glycosylation process.

BM-03 ANALISIS OF THE GLYCOSYLATION IN CHOLINESTERASES EXTRACTED
OF NONALCOHOLIC FATTY LIVER FROM MOUSE TREATED WITH
HYPERCHOLESTEROLEMIC DIET
Morales-Arroyo I1*, Quezada-Martnez R1*,Lpez-Durn RM1, Garca-Surez MD2, Salinas-Arreortua
N1, Carbajal-Aguilera K3, Serrano H1, Gutirrez-Ruiz MC1, Gmez-Olivares JL1. Departamentos de
Ciencias de la Salud1, Biologa2, Biologa de la Reproduccin3. Posgrado en Biologa Experimental*.
Universidad Autnoma Metropolitana-Iztapalapa. Instituto Nacional de Pediatra, SS. Mxico.
Ivis.ibrahim@hotmail.com
Av. San Rafael Atlixco No.186 Col. Vicentina C.P. 09340 Iztapalapa Mxico DF.

Nonalcoholic fatty liver disease (NAFLD) is the build up of extra fat in liver cells that is
not caused by excessive alcohol use. It is normal for the liver contains some fat.
However, if more than 5% -10% percent of the livers weight is fat has been
considerated as steatosis. Mammalian posses two cholinesterases (ChEs):
acetylcholinesterase and butyrylcholinesterase. The liver represents source from
butyrylcholinesterase. Both ChEs are glycoproteins with difference N-glycosylation sites
from N-glycosylation. The purpose of this study was to estimate the activities acetyl -
and butyrylcholinesterase and its glycosylation in non-alcoholic fatty liver disease in
mice. Two grupos were formed: a) Control with normal diet Chow and b) Treated with
experimental hypercholesterolemic diet (Dietz, Inc.). Ches activities from extracts
without or with detergent were estimated. Mixture from S1+S2 extracts were incubated
with lectins from distinct specificity by 16-18 h. Results showed low values of liver
cholinesterases, is has frequently been reported in sera of patients suffering from liver
disease. The liver is an important source of Ache activity. Altered Ches levels may be a
useful biomarker for liver disease. Percentage interactions were different on ChEs from
control mice and mice treated with hypercholesterlemic diet. The possible role of post-
translational modifications such as subunit oligomerization, protein glycosylation and

92
oligosaccharide processing imply that of N-glycosylation sites plays a role in the
circulatory life-time of the enzymes.

BM-04 DYNAMICS OF THE CARBOHYDRATES ON SPERM PLASMA MEMBRANE OF
Corynorhinus mexicanus BAT AS A SPERM MATURATION MODEL.
Rodrguez-Tobn Ahiezer
1
, Fierro Reyna
2
, Corts Barberena Edith
2
, Len-Galvn Miguel A.
3
and Arenas-Ros
Edith
4
.
1
Doctorado en Biologa Experimental,
2
Departamento de Ciencias de la Salud,
3
Departamento de
Biologa, and
4
Departamento de Biologa de la Reproduccin. Universidad Autnoma MetropolitanaIztapalapa.
San Rafael Atlixco No. 186, Col. Vicentina, CP 09340. City. Mxico Phone: 58044923. Mail:
ahiezerrod@yahoo.com.mx.

The glycosylations in the sperm plasma membrane are correlated with epididymal sperm
maduration and acquisition of fertilizing capacity. The bat Corynorhinus mexicanus is a good
model for studying the sperm maturational process due to it has a prolonged epididymal
sperm storage period. Therefore has been determined the distribution of mannose, N-
acetylglucosamine, sialic acid and fucose residues in the sperm plasma membrane of C.
mexicanus at different times during maturation. We obtained sperm samples from the caput,
corpus and cauda of epididymis of 12 bats captured from September to October. To identify
the distribution of sugars were used lectins-FITC: WGA, Con A and UEA. Fluorescence
intensity (FI) was measured in 5x10
6
cells by flow cytometry. No significant changes were
found in the FI between epididymal regions in any of the study dates marked with WGA.
However, we found a decrease in the FI of WGA in the cauda of epididymis from September
24. Upon the sperm entering at the epididymis the FI calculated for UEA presents a gradual
increase thus remaining as time goes on storage mainly in the caput. The Con A FI was
constant in the three regions the epididymis, decreasing during time of the storage in the
caudal region. According the results we concluded that glycosylations of proteins found in
sperm membrane of C. mexicanus are related to the epididymal maturation process, which in
this species ends in the caudal region.

93
Supported by CONACYT (0105961/I0110/194/09) and UAM, MEXICO. A. Rodrguez-Tobn is supported by a
CONACYT scholarship, number: 332837.

BM-05 EFFECT OF -AMYLOID 25-35 PEPTIDE ON THE O-GlcNACYLATION AND
PHOSPHORYLATION TAU PROTEIN IN HYPERGLICEMIC RATS.

Liliana Lozano
a
, Noe Alvarado-Vsquez
*a,b
, Viviana Zomosa
c
, Daniel Limon
d
, Tony Lefebvre
e
, Edgar
Zenteno
a
, and Jorge Guevara
*a
.
a
Bioqumica, Facultad de Medicina, Universidad Nacional Autnoma de Mxico, D.F., Mxico.
b
Bioqumica, Instituto Nacional de Enfermedades Respiratorias, Mxico.
c
Laboratorio Experimental de Enfermedades Neurodegenerativas, INNN, Mxico, DF, Mxico.
d
Laboratorio de Neurofarmacologa, Benemrita Universidad Autnoma de Puebla, Puebla, Mxico.
e
UMR-CNRS 8576, UGSF, USTL, IFR 147, 59655 Villeneuve dAscq, France.

Hyperglycemia is considered a risk factor to progress of Alzheimers disease (AD), to
promoter of amyloid beta (A) aggregation, and hyperphosphorylation of tau protein.
The phosphorylation of Tau protein is regulated by O-GlcNAcylation; however, in
presence of high glucose has been reported a major activity of glycogen-synthase-
kinase-3 (GSK3), one of the main kinases involved in the phosphorylation of tau
protein. In this work, we evaluated the effect of 25-35A peptide in phosphorylation/O-
GlcNAcylation of tau, and on GS3K expression in brain of rats treated with
streptozotocin. Four groups of rats were studied: control, A25-35 peptide, A25-35
and STZ, and STZ only treated. Additionally, were evaluated the weight, glucose
concentration, glycosylated hemoglobin % (HbA1c) and insulin. The O-GlcNAcylation
was determinate in hippocampal-brain area by immunofluorescence using lectin Wheat
Germ Aglutinin (WGA); and the phosphorylatin for P-Tau-Thr-231, P-Tau-Ser-396, and
GSK3 were determined using specific antibodies. Our results showed statistically
significant differences between groups treated/or not treated with STZ in the mean
weight, glucose and insulin concentration, and HbA1c percentage. The results of
immunofluorescence showed a diminution of O-GlcNAcylation, linked with an increase
of phosphorylation to P-Thr-231, P-Ser-396 of the Tau protein, mainly in treated animals
with AB and STZ. Also, an enhanced expression of GSK3 was observed. Therefore,
our findings suggest that the damage generated by the presence of hyperglycemia in

94
association with A25-35, induce a major phosphorylation of tau protein that
consequence of over-expression of GSK3.
Key words: Alzheimer disease; tau protein, amyloid , OGT, O-GlcNAcylation, Glycogen
synthase kinase 3.

IN224011 PAPIIT M09S02 CONACYT.

BM-06 RELEVANCE OF PROTEIN GLYCOSYLATION PATHWAYS FOR IMMUNE
SENSING OF Candida guilliermondii.
Navarro-Arias Mara de Jess
1
, Lpez-Prez Mercedes Guadalupe
2
, Mora-Montes Hctor Manuel
1,
*.
1
Departamento de Biologa, Divisin de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad
de Guanajuato, Noria Alta s/n, Col. Noria Alta, C.P. 36050, Guanajuato, Gto., Mxico.
2
Departmento de
Biotecnologa y Bioqumica, CINVESTAV-Irapuato, C.P. 36821, Irapuato, Gto., Mxico. * Corresponding
author: tel 473 732 0006, ext. 8154; email: hmora@ugto.mx

Candida guilliermondii is an opportunistic fungal pathogen usually associated with
immunocompromised patients, is considered less virulent than C. albicans but is one of
the main emergent species of this genus. The cell wall is the first site of interaction
between the fungi and host cells, so this structure is involved in triggering the immune
defense system. The composition and organization of C. guilliermondii cell wall is so far
unknown, but it is assumed that it is similar to that found in C. albicans; where it is
composed by an inner layer of chitin and -glucans, covered with an outer layer of
highly glycosylated proteins (N- and O- mannans). Mannans play important roles in cell
wall integrity, adhesion to host tissues, virulence and during the establishment of a
protective host immune response. The aim of this work is to elucidate the composition
and organization of C. guilliermondii cell wall and its role during immune sensing by
human monocytes. Where the results indicated that the fungus-immune cell interaction:
C. guilliermondii is more recognized that C. albicans this may be due to the morphology,
since it does not form hyphae C. guilliermondii or the composition and organization of
the cell wall may vary with respect to C. albicans and therefore recognition (experiments
to be done).

95

This work is supported by CONACYT (CB2011-166860) and Universidad de Guanajuato.

BM-07 MODELING A TEPARY BEAN (Phaseolus acutifolius) LECTIN FROM THE
DEDUCED POLYPEPTIDE SEQUENCE OF A mRNA EXPRESSED IN E. coli.
Martnez-Alarcn D
1
, Castro-Guilln JL
2
, Cruz-Hernndez A
1
, Campos-Guilln J
1
, Ferriz-Martnez R
1
,
Blanco-Labra A
2
, Garca-Gasca T
1
. 1. Facultad de Ciencias Naturales. Universidad Autnoma de
Quertaro. Av de las Ciencias s/n. Juriquilla, CP 76230, Qro. Mxico. Tel 4421921200 ext 5308. 2.
Departamento de Bioqumica y Biotecnologa de Plantas. 2. Centro de Investigacin y de Estudios
Avanzados del Instituto Politcnico Nacional, Unidad Irapuato, Departamento de Biotecnologa y
Bioqumica. Km 9.6 Libramiento Norte Carretera Irapuato-Len, CP 36821. Irapuato, Gto. Mxico. Tel
4626239640. alejandroblancolabra@gmail.com, tggasca@gmail.com

We have previously demonstrated the anticancer effect of Tepary bean lectins. In order
to know the molecular structure of at least one of those lectins, the deduced polypetide
sequence of one cloned cDNA was modeled. First, RNA was obtained and then a PCR
was performed from the cDNA using 4 oligos: (F1) ATGGCTTCCTCCAACTTCTCCA,
(R1) TAGAGGATTTGGTTGAGGACGA (F2) CCTCTTCCTTCCGCTTCTCAC and (R2)
AAGAGAGGAGGTCGTTCGTTTC in all possible combinations. The amplified product
for F1 and R1 was chosen, purified, and sequenced in both directions, and the one with
the highest homology to the common bean phytohemagglutinin was ligated into a TOPO
2.1 sequencing vector. A total of 19 transformed bacteria were positive for the lectin
gene, then, PCR was run for each one of them. From the purified plasmids, three
samples were sequenced. Alignments were performed in sense and antisense and a
consensus sequence with high homology to that reported by Mirkov et al. (1994) for
Phaseolus acutifolius phytohemagglutinin was obtained. By modeling the deduced
protein from the sequence of the obtained transcript it was found that the three-
dimensional structure has the same highly conserved structure of the lectins that
showed very high identity. As part of this structure, -sheet and an interaction domain
with calcium and manganese were found. In addition, the modeling also confirmed the

96
high variability among possible glycosylation sites and that the most likely site of
glycosylation is near the calcium-manganese domain. This data strongly suggests that
the cloned transcript belongs to a lectin of Phaseolus acutifolius.
Key words: cDNA cloning, Lectin, Phaseolus acutifolius, protein modeling

BM-08 GLICOPROTEOMICS OF THE MIDGUT LARVAL OF Zabrotes subfasciatus
ASSOCIATED TO THE INSECTICIDAL MECHANISM OF PF2 LECTIN FROM Olneya
tesota.
Lagarda-Daz Irlanda
1
, Guzmn-Partida Ana M.
1
, Robles-Burgeo M. Refugio
1
, Geiser Dawn
2
, Winzerling
Joy
2
and Vzquez-Moreno Luz
1
.
1
Centro de Investigacion en Alimentacion y Desarrollo, A.C. Apartado
Postal 1735, Hermosillo, Tel. (662) 2895400.
2
Department of Nutritional Sciences, University of Arizona,
Tucson, Arizona 8572. lvazquez@ciad.mx
.
The gut of insects, as well as other animals, is rich in glycoconjugates that have
important biological functions required for growth and development. Lectins can bind to
these glycoconjugates and induce various insecticidal effects such as blocking of
receptors or transporters and inactivation or activation of enzymes. PF2 is a lectin
isolated from the seeds of Olneya tesota (Palo Fierro), and was recently reported to
cause 100% mortality for Zabrotes subfasciatus larvae (bean pest). In this study we
evaluated recognition of the PF2 lectin to -amylases from Z. subfasciatus midgut and
the effect of PF2 on -amylase activity. Results showed that PF2 prompt a decrease of
total amylase activity. In-gel assay for amylase activity displayed three enzyme
isoforms, but only one isoform was recognized by PF2. The identity of this Z.
subfasciatus -amylase was confirmed by LC-MS/MS. In addition, we evaluated the
binding of PF2 to midgut glycoproteins at different larval stages using techniques such
as chromatography, 2D-gel electrophoresis and lectin blotting. Lectin interaction
revealed differences in glycoprotein patterns at different larval stages. Analysis of LC-
MS/MS and searching in insect databases showed that several glycoproteins could act
as targets for PF2 recognition. Each of these proteins is physiologically important in
such a way that could be associated to the insecticidal mechanism of PF2.

97

BM-09 O-GLYCOSYLATION EXPRESSION IN CERVICAL CANCER CELL LINES BY
Amaranthus leucocarpus LECTIN.
Illescas Barbosa D.
1,3
, Cruz Santiago J.
2,3
, Aquino Gil M. O.
3,5
, Prez-Cervera Y.
1,3
, Zenteno Galindo
E.
3,4
. Solrzano-Mata C. J.
1,3
*. Facultad de Odontologa UABJO
1
, Facultad de Medicina y Ciruga
UABJO
2
, Centro de Investigaciones Multidisciplinarias UNAM-UABJO
3
, Departamento de Bioqumica,
Facultad de Medicina UNAM
4
, Instituto Tecnolgico de Oaxaca
5
. *email: universidad99@hotmail.com

Introduction: The changes in the O-glycosylation is a common event in cervical cancer
(CaCu), the identification of cell lines expressing these modifications glycoconjugates
are useful as models for the study of molecules with potential as tumor markers or
therapeutic targets. Objective: Identifying the expression and cellular localization of O-
glycosylation detected by Amaranthus leucocarpus lectin (ALL) CaCu cells lines and
compared with epithelial cells healthy cervical tissue. Methodology:
Immunocytochemical and western blot performed with cell lines CaLo, Caski, SiHa,
ViBo and healthy cervical tissue biopsies with ALL lectin. Results: ALL lectin strongly
acknowledged the plasma membrane and cytoplasmic granules moderately cytoskeletal
structures in cells Calo, Caski and SiHa, in contrast ViBo cells recognizing the plasma
membrane was lower and showed a greater recognition of cytoplasmic structures.
Cervical epithelial cells healthy showed mild recognition at the plasma membrane,
specifically the intermediate layer and surface staining of the stratum basal layer
showed no staining in the cytoplasm. The western blot showed bands between 10 and
200 kDa, the recognition pattern was similar between cell lines, except for ViBo.
Conclusion: The results showed changes in the location and intensity on expression of
O-glycosidically linked glycans in CaCu cells lines that differ in healthy tissue,
suggesting the importance of the purification and characterization to identify their
participation in tumor progression and use as tumor markers or therapeutic targets.

This project was supported by PROMEP/103.5/12/3782 UABJO, Mxico.

98

BM-10 INTERACTION OF PLANT LECTINS WITH Aspergillus parasiticus.
Daz-Garca E.J.
1
, Isidro-Lzaro J.
1
, Pina-Canseco M.S.
2
, Snchez-Medina M.A.
1
, Prez-Santiago A.D
1
.
1
Instituto Tecnolgico de Oaxaca, Av. Vctor Bravo Ahuja No. 125 Esq. Calz. Tecnolgico, Oaxaca, Oax.
C.P. 68030.
2
Universidad Autnoma Benito Jurez de Oaxaca, Carretera Antigua a San Felipe del Agua
S/N. Oaxaca. C.P. 6820. edg.89@live.com.mx

Aspergillus fungus damages many food products; an important case is the deterioration
in grain stored, which also produces highly carcinogenic compounds called aflatoxins.
Among the most important species causing this damage, they include: A. flavus and A.
parasiticus. Several studies have shown the interaction of lectins with different
microorganisms. The purpose of this study was evaluating the interaction of corn
coleoptile lectin and sorghum lectin (Sorghum bicolor) with the fungus A. parasiticus.
This was made by activation of A. parasticus strains and extracts of lectins, these
extracts were tested through Bradford protein quantification and hemagglutination
activity, the interaction and the effect of lectins were evaluated using three bioassays:
colony forming units (UFC), inoculation pitting and halos of inhibition. The results
obtained during plaque bioassays demonstrate that corn coleoptile lectin showed a
growth activating effect of A. parasiticus. Furthermore, the lectin of sorghum extract
inhibited the development of the fungus.

99

BM-11 CONTENT OF PICEID FROM MEXICAN GRAPE AND WINE (CABERNET
SAUVIGNON).
Acua Avila Pedro Estanislao
1
.Lopz Cortz Ma. del Socorro
1
,Vasquez Murrieta Mara Soledad
2
1 .
Depto. Biofsica 2. Depto. De Microbiologa Industrial. Escuela Nacional de Ciencias Biolgicas. Instituto
Politcnico Nacional Prolongacin de Carpio y Plan de Ayala s/n, Col. Santo Toms, C.P. 11340, Mxico,
D.F. pacua2@gmail.com

Many researches have been demonstrated that resveratrol provides health benefits,
such as the prevention of cardiovascular diseases, arteriosclerosis and cancer. One
source of resveratrol is the red wine; in fact, epidemiological studies indicated an
inverse relation between moderate wine consumption and the risk of coronary heart
disease, the so-called French Paradox. On wines and grapes, resveratrol is also
present as his glycoside form call piceid. The aim of this work was to evaluate the
content of piceid on red grapes and red wine from two different regions of Mexico.
Piceid was determinate by HPLC on elution gradient water-acetonitrile on acetic acid
(0.5 %). Red grapes from Queretaro (QR
Red wine from QRO had the major level of piceid (2.08 0.02 mg/ L), wine from VAL
(0.363 0.03 mg/L) and COL (0.817 0.02 mg/L). Thus results indicated that the red
grape and the red wine from QRO had major content of piceid than the grapes Baja
California. This result is consistent to the total polyphenol content and antioxidant
activity that was previously reported to the same grapes.

BM-12 INDUCTION OF GLYCOSYLATION AND MORPHOLOGICAL
DIFFERENTIATION BY DEXAMETHASONE AND ALL-TRANS RETINAL IN C6
CELLS.

100
Della-Valle Daniela, Espinosa Blanca, Calvillo Minerva , Guevara Jorge Faculty of Medicine,
Postgraduate Department of Biological Sciences, National Autonomous University of Mexico. 2
Biochemistry Department, Faculty of Medicine, National Autonomous University of Mexico 3 Biochemisty
Department, National Institute of Respiratory Diseases. 4. Neurodegenerative Disorders Laboratory,
National Institute of Neurology and Neurosurgery Manuel Velasco Suarez. Av Insurgentes Sur 3877, La
Fama, Tlalpan, C.P 14269, Mexico Distrito Federal, Mexico. 52 (55) 56232510,
jorge.guevara@comunidad.unam.mx

Glycosylation is a cells major protein-post-traductional change that allows it to have
structural and modulatory functions. It is common to find specific temporal and spatial
patterns of glycans expression in relation to cellular activation, differentiation,
inflammation and neurodegenerative disorders such as Alzheimers disease, in which
astrocyte related inflammation has been observed. C6 cells are a rat glioma cell line
that expresses specific astrocyte biological markers (S100 Protein and GFAP).
Glucocorticoids antiproliferative effects and Retinoids growth-promoting and
differentiation effects have been observed. This work aims to identify the effects over
glycosylation and morphological differentiation in C6 cells by Dexamethasone and All-
Trans Retinal (ATRA) in order to establish a relation between these effects and either
inflammatory or anti-inflamatory activity.Methodology: Cell culture, Dexamethasone or
ATRA addition (5M, 10M, 20M, 75% confluency), 3 or 5 days post-treatment
standard lectin cytochemistry (PNA, MrL, SNA, ALL, MAA and WGA), fluorescence
microscopy.Results: Time-dose dependent presence of glycans in C6 cell cultures when
treated with either Dexamethasone or ATRA. Intensity of the recognition varies
according to the drug, the concentration and the lectin used in the culture and
cytochemistry.Conclusions: Significant variations between quantity of mark observed
and drug used were noted, while ATRA acutely induces fibroblast-type morphology and
an increase in the lectin recognition, Dexamethasone slows these morphological
changes and only inducts an increase in lectin recognition with high doses.
Complementary experiments will be conducted to determine the specific roll of these
glycan changes and their relation with an inflammatory response in this cell line.

101
BM-13 A FUNCTIONAL SPLICING VARIANT OF CMP-SIALIC ACID TRANSPORTER
Roberta Salinas-Marin,
1
Rosella Mollicone,
2
Ivan Martinez-Duncker
1
1
Human Glycobiology Laboratory, Science Faculty, Autonomous University of the State of Morelos,
Avenida Universidad 1001,Col. Chamilpa, 62209 Cuernavaca, MOR, Mexico.
2
INSERM 1004, Hpital P.
Brousse, University of Paris Sud XI, Villejuif, France.duncker@uaem.mx

Introduction.The human Golgi CMP-sialic acid transporter (SLC35A1) is responsible for
translocation of CMP-Sialic acid, the donor substrate of sialyltransferases, into the Golgi
lumen. The SLC35A1 generates a wild type isoform and also shorter isoforms by
alternative splicing, including the d177 isoform (skipping of exon 6). Among all the
isoforms, the d177 is the most similar to the wild-type protein but its function has not
been evaluated.
Methodology. HepG2 (Human hepatocellular liver carcinoma cell line) cells were
transfected with antisense morpholinos (MO) to knockdown the SLC35A1 wt isoform at
the expense of inducing the d177 isoform. The cell surface de novo cell sialylation
profile of MO treated HepG2 cells was evaluated with azide-modified mannosamine
(ManNAz). Additionally, the d177 gene was incorporated into a vector and Lec2 cells
deficient in CMP-Sialic acid transport were transfected in order to asses
complementation.
Conclusion. The SLC35A1 d177 isoform was as efficient as the wt isoform in
maintaining surface de novo sialylation in HepG2 cells and was able to complement the
Lec2 mutant cell line. We conclude that the region coded by exon 6 is not essential for
function.

BM-14 RECOGNITION AND DIFFERENTIAL AGLUTINATION OF CERVICAL CANCER
CELLS WITH A NOVEL LECTIN FROM TUNA SHELL. Patio Morales Carlos Csar 1,
Serrano Luna Armando Levith
1
, Chavelas-Adame Eneas Alejandro
1
, Castaeda

102
Saucedo Eduardo
2
.
1
Laboratorio de Bioqumica Termodinmica y Estructura de
Protenas and
2
Laboratorio de Biologa Celular del Cncer, UA
Guerrero.carlosesar@gmail.com, eneas_02@yahoo.com.mx
Oncogenicity is associated in altered carbohydrate profiles in tumor cells membrane.
Lectins are useful tools to recognize specifically carbohydrates and for differential
recognition of tumor cells. Besides its been described that many lectins showed effects
on proliferation and cell viability. In this work we identified a protein fraction from tuna
shell which displayed agglutinating activity toward tumor cell lines and also effected cell
proliferation in culture cells. The tuna fraction was obtained after precipitation of saline
extract with (NH4) 2SO4 at 40% saturation, agglutination assays of the extracts on
three cell lines derived from cervical cancer and a non-tumorigenic cell line was tested.
Assays were performed with violet crystal to observe the effect on the proliferation of
cells in culture. Results showed that tuna extract agglutinates preferentially C33-A
human cell line, this effect was inhibited with GlcNMAc and NeuAc at 200 mM;
whereas, other simple carbohydrates showed no effect on agglutination activity in this
nor in other cervical lines. Our results strongly suggested that tuna extracts represented
a powerful tool to study cervical cancer cells.

BM-15 COSTIMULATION MEDIATED BY N-ACETYL-GALACTOSAMINE ON MURINE
CD3-ACTIVATED CD4
+
T CELLS

Arenas-Del ngel MC
1
, Chvez R
2
, Zenteno E
2
, Lascurain R
1,2
.
1
Depto. de Investigacin en Bioqumica, Instituto Nacional de Enfermedades
Respiratorias.
2
Depto. de Bioqumica, Universidad Nacional Atonoma de Mxico. Tel:
01(55)54871705.

INTRODUCTION. N-Acetyl-D-Galactosamine (GalNAc) in the Galactose(Gal)1-3GalNAc-R
disaccharide is recognized by Amarathus leucocarpus lectin (ALL), which binds a 70 kDa
glycoprotein on murine CD4
+
T cell surface. This glycoprotein is related with CD4
+
T cell
activation and in culture of total cells from murine lymph node has suggested an effect
costimulatory. OBJETIVE. To analyze costimulatory effect of N-Acetyl-Galactosamine
(GalNac) recognized by ALL in purified CD4
+
T cells activated via CD3 and in this condition to
evaluate the cytokines production, besides to analyze the cell membrane glycoproteins
recognized by ALL. MATERIAL AND METHODS. Six-to eight-week old male Balb/c mice

103
were killed by cervical disruption and CD4
+
T cells from axial lymph nodes were obtained by
negative selection using a mAbs-coated magnetic microbeads kit. Cells were stained with CFSE
dye and cultured with either plate-immobilized anti-CD3 mAb alone, or in the presence of anti-
CD28 mAb or ALL for 48 h at 37C in a 5% CO
2
atmosphere. After culture, proliferating cells
were analyzed by flow cytometry, cytokines mRNA production was evaluated by RT-PCR. In
addition, these cells were lysed and lipid rafts isolated by gradient ultracentrifugation, then
protein fractions were examined by two-dimensional electrophoresis and incubated with ALL in
a far-western blot. Finally, protein spots were analyzed by mass spectrometry. RESULTS.
Purified CD4
+
T cells stimulated at 1g/ml of anti-CD3 mAb, showed 39.5% 0.6 proliferation,
which increased 1.7 fold (69.1% 0.9) when these cells were costimulated with 5g/ml ALL (P
= 0.03). The costimulation of CD4
+
T cells by ALL was comparable to those induced via CD3
plus 1g/ml CD28 at 48 h of culture (63.6% 8.1). In contrast, cells cultured with anti-CD28
mAb plus ALL or with these reagents separately were not able to induce cell proliferation.
Concerning to cytokines mRNA synthesis, the cells costimulated by ALL produce transcripts to
IL-2 and TGF-, but no to IL-4, IFN- nor TNF-. By means of mass spectrometry analysis, the
main ALL-recognized lipid raft glycoprotein showed 41% homology with an unnamed protein
product (gi 74186081 from NCBI) that belongs to the Ezrin/radixin/moesin (ERM) family and
exhibit a theoretical mass of 67.8 kDa. ERM family proteins are actin-binding proteins that act as
linkers between the actin cytoskeleton and plasma membrane proteins. CONCLUSION. The
ALL-recognized GalNAc-bearing glycoprotein appears to be involved in the generation of
costimulation signals in CD3-activated CD4
+
T cells.

BM-16 THE VITAMIN D RECEPTOR IS TARGET OF O-GLcNAc GLYCOSYLATION
UNDER HYPERGLYCEMIC CONDITIONS IN THP1-CELLS. Hernndez-Snchez
Fernando
1
, Balderas-Anaya Madeline
1
. Vega-Hernndez Ricardo
1
and Torres Martha
1
.
Departamento de Microbiologa, INER
1
, Mxico DF. Tel 54871700.
marthatorres98@yahoo.com.
Vitamin D plays an important role as regulatory hormone in calcium metabolism and in
glucose homeostasis and insulin secretion through transcriptional mechanisms
mediated by its receptor (VDR). Interestingly, addition of -D-N-acetyl glucosamine
residues to proteins, mediated by the O-GlcNAc transferase (OGA) has been implicated
in the glucose toxicity and in the promotion of insulin resistance in mice models of
diabetes. However it is not known if the VDR is target of the OGA enzyme.
OBJECTIVE: In this work we intend to elucidate if the VDR is O-GlcNAcylated in the
pro-monocytic cell line THP1 grown under increasing glucose concentrations.
METHODS: THP1 cells were cultured at 5.5 mM, 11mM, 20mM and 30mM of glucose
for 24h, and the total cell lysates were obtained. Afterwards the VDR was isolated
through immune precipitation, and then electrophoresed and transferred to PVDF
membranes. The protein glycosylation was revealed by Western Blot using an antibody
that recognizes O-GlcNAc residues. RESULTS: Two main VDR isoforms were
detectable in total extracts: one of 50kDa and one of 100kDa but their relative amounts

104
remained unaffected by glucose; nonetheless the 100kDa isoform was glycosylated at
30 mM. CONCLUSION: A high molecular weight VDR which had not been previously
reported is target of O-GlcNAcylation. A further characterization of the 100kDa isoform,
and its implications on diabetes, should be addressed.

BM-17 INSULIN SIGNALING CONTROLS THE EXPRESSION OF O-GLCNAC
TRANSFERASE AND ITS INTERACTION WITH LIPID MICRODOMAINS.
Aquino Gil M. O.,
2
Perez-Cervera Y.,
1
,
2
Dehennaut V.,
1
Guedri K.,
1
Solrzano Mata C. J.,
1
,
2
Olivier-Van
Stichelen S.,
1
Michalski J. C.,
1
Foulquier F.,
1
Lefebvre T.
1
,
1
Centre National de la Recherche Scientifique
(CNRS)Unit Mixte de Recherche (UMR) 8576, Unit of Structural and Functional Glycobiology, Institut
Fdratif de Recherche (IFR) 147, University of Lille 1, Villeneuve dAscq, France; and
2
Centro
Multidisciplinario de Investigacin, Universidad Nacional Autnoma de Mxico (UNAM)Universidad
Autnoma Benito Jurez de Oaxaca (UABJO), Oaxaca, Mexico. Email: yobanper@gmail.com

Lipid microdomains (rafts) are cholesterol enriched dynamic ordered lipid domains
belonging to cell membranes involved in diverse cellular functions, including signal
transduction, membrane trafficking, and infection. Many studies have reported
relationships between insulin signaling and lipid rafts. Likewise, links between insulin
signaling and O-Glc-NAcylation have also been described. However, the potential
connection between O-GlcNAc and raft dynamics remains unexplored. Here we show
that O-GlcNAc and the enzyme that creates this modification, O-GlcNAc transferase
(OGT), are localized in rafts. On insulin stimulation, we observe time-dependent
increases in OGT expression and localization within rafts. We show that these
processes depend on activation of the phosphatidylinositol 3-kinase (PI3K) pathway.
Inhibition of OGT does not significantly affect cholesterol synthesis and raft building but
decreases insulin receptor expression and PI3K and mitogen activated protein kinase
pathway activation. Taken together, these findings indicate that O-GlcNAcylation, lipid
rafts, and signaling pathways are spatiotemporally coordinated to enable fundamental
cellular functions.

105

BM-18 COMPARATIVE STUDY OF THE EXPRESSION OF BETA-GLUCOSIDASE
GENES (glu1 AND glu2) IN TEOSINTE Z. parviglumis, Z. diploperennis, Z.
mexicana AND Z. luxurians. Matias Cervantes CA
1
, Pina Canseco MS
2
, Hernndez Cruz P
2
,
Prez-Campos E
1
, Cisneros Solano A
3
, Aragn Cuervas F
4
and Martnez Cruz M
1
.
1
Unidad de Bioqumica e Inmunologa. Instituto Tecnolgico de Oaxaca. Av. Ing. Vctor Bravo Ahuja, No.
125, Esq. Calz. Tecnolgico, C.P. 68030, Tel., 01 (951) 516 97 58, Oaxaca de Jurez, Oaxaca.
2
Centro
de Investigacin en Ciencias Mdicas y Biolgicas, UABJO.
3
Escuela de Medicina Veterinaria y
Zootecnia.
4
Instituto Nacional de Investigaciones Forestales, Agrcolas y Pecuarias, Campus Oaxaca.
E-mail: carloscervantes.ox@outlook.com, martinezcu9@hotmail.com

INTRODUCTION. In maize, there have been identified and characterized two
isoenzymes of -glucosidase known as Glu1 and Glu2 which are encoded by the glu1
and glu2 genes, respectively. The main function of -glucosidase in maize is related to
defense mechanisms. We are interested in identifying and characterizing the Glu1 and
Glu2 isoenzymes in some species of teosinte.
MATERIALS AND METHODS. The seeds of teosinte diploperennis, parviglumis,
mexicana and luxurians were germined. At the fifth day of growth, total RNA was
extracted using TRIzol reagent. First-strand cDNA synthesis was performed using the
One-step RT-PCR kit (Qiagen), using glu1 and glu2 primers reported in maize. The
PCR products were analyzed on a 2.5% agarose gel with electrophoresis conditions of
80V for 90 min. The maize actin gene Mac1 was used as an internal control.
RESULTS. Approximately 100 bp and 150 bp were the amplicon sizes of glu1 and glu2,
respectively. The sequence of glu1 amplicons showed 100% identity with the signal
peptide of Glu1 maize protein. The sequence of glu2 amplicons showed 100% identity
with the coding sequence of Glu2 maize protein (exons 3, 4 and 5). We identified a
change of a cytosine by a thymine that did not affect the amino acid sequence in
Z. diploperennis.
CONCLUSIONS. We have identified conserved regions of the gene glu1 and glu2 in
teosinte parviglumis and diploperennis, its thought that the defense mechanism in
maize has been preserved for more than ten thousand years.

106

D-01 EXPRESSIN OF ANTIGEN T AND GALECTIN-3 IN TESTIS APPENDIX IN
CHILDREN WITH CRYPTORCHIDISM
De la Cruz Castillo Doris Y
1
, Gallegos Velasco Belm
1
, Prez Campos E
2
y Hernandez Cruz P*
1

1
Laboratorio de Glicobiologa CICIMEBIO, Facultad de Medicina y Ciruga, Universidad Autnoma Benito
Jurez de Oaxaca.
2
Unidad de Investigacin en Bioqumica, Instituto Tecnolgico de Oaxaca
*fuegoblanco136@yahoo.com.mx

INTRODUCTION: The testis appendix, are embryological remnants composed of loose
connective tissue. Clinically are structures with biological activity, which can result from
ischemia, metaplasia or degeneration after, given stimulus. The undescended testicles
can be an important factor of this stimulus. Because disease is associated with damage
to the gonadal cells, (infertility and increased risk cancer). The glycosylated antigen
expression is associated with the development of cancer and with the capacity of the
tumor cell to evade the immune system. Structures such as the antigen T (Gal
1,3GalNAc1-O-Ser/Thr) and Tn (GalNAc1-O-Ser/Thr). These antigens are expressed
in the normal manner in the mucin, or mucin type glycoproteins, which can be
synthesized in an incomplete manner. OBJECTIVE: Determine the expression of T
antigen and Galectin 3 in testicular appendices in children with cryptorchidism, using
histochemistry. METHODOLOGY: 10 Paraffin-embedded blocks from testis appendix
was cut in 6 m-thick sections. Select sections were labeled with lectins (1g/ml) or
antigalectin-3 (dilution 1:100) overnight at 4C, the samples were labeled with
streptavidin-peroxidase (1:1000 in PBS) for 1 h at 37C. Unbound conjugate was
removed by washing 6 times with PBS. The binding of lectin was revealed by incubating
with 3-amino-9-ethyl-carbazole (AEC), Double labeling of slides were performed as
DESRDENES DE LA GLICOSILACIN Y ENFERMEDADES RARAS
(D)

107
follows: Tissue samples were labeled with lectins (1g/ml) overnight at 4C and
monoclonal anti galectin-3 only that lectin binding was indirectly recognized with
extravidin-FITC conjugated and visualized using green filter. Anti Galectin antibodies
were revealed with extravidin-Red-X conjugate. Slides were observed with AXIOSCOP
40 microscope equipped with digital camera AXIOCAM MRC and micrographs analysed
with ZEN pro 2011 Software. RESULTS: Lectins from Artocarpus integrifolia y Arachis
hypogaea and galectin-3 recognized epithelial and estromal cells. CONCLUSIONS: Our
results suggest that expression of antigen T and galectin 3 seems to participate in
development of testis appendix in children with cryptorchidism

D-02 EXPRESSIN OF MUCINS 1, 2, 5, TA998, DF3 AND GALECTIN-3 IN TESTIS
APPENDIX IN CHILDREN WITH CRYPTORCHIDISM
Rios Lpez Karen I
1
1
, Prez-Campos E
2
y Hernndez Cruz P*
1
.
1
Laboratorio de Glicobiologa CICIMEBIO, Facultad de Medicina y Ciruga, Universidad Autnoma Benito
Jurez de Oaxaca.
2

INTRODUCTION: The testis appendix, are embryological remnants composed of loose
connective tissue. Clinically are structures with biological activity, which can result from
ischemia, metaplasia or degeneration after, given stimulus. The undescended testicles
can be an important factor of this stimulus. Because disease is associated with damage
to the gonadal cells, (infertility and increased risk cancer). The glycosylated antigen
expression is associated with the development of cancer and with the capacity of the
tumor cell to evade the immune system. Structures such as the antigen T (Gal
1,3GalNAc1-O-Ser/Thr) and Tn (GalNAc1-O-Ser/Thr). These antigens are expressed
in the normal manner in the mucin, glycoproteins, which contain in its structure more
than 80% of O-glycans, which can be synthesized in an incomplete manner.

108
OBJECTIVE: To identify the expression of mucins in testis appendix in children with
cryptorchidism using lectin-histochemistry. METHODOLOGY: 10 Paraffin-embedded
blocks from testis appendix was cut in 6 m-thick sections. Select sections were labeled
with lectins (1g/ml) or antigalectin-3 (dilution 1:100) overnight at 4C, the samples
were labeled with streptavidin-peroxidase (1:1000 in PBS) for 1 h at 37C. Unbound
conjugate was removed by washing 6 times with PBS. The binding of lectin was
revealed by incubating with 3-amino-9-ethyl-carbazole (AEC), Double labeling of slides
were performed as follows: Tissue samples were labeled with lectins (1g/ml) overnight
at 4C and monoclonal anti galectin-3 only that lectin binding was indirectly recognized
with extravidin-FITC conjugated and visualized using green filter. Anti Galectin
antibodies were revealed with extravidin-Red-X conjugate. Slides were observed with
AXIOSCOP 40 microscope equipped with digital camera AXIOCAM MRC and
micrographs analysed with ZEN pro 2011 Software. RESULTS: Mucins and galectin-3
recognized epithelial and estromal cells. CONCLUSIONS: Our results suggest that
expression of mucin 1, 2, 5, TA998, DF3 and galectin 3 seems to participate in
development of testis appendix in children with cryptorchidism

D-03 RECOGNITION OF Tritricum vulgaris LECTIN IN PLATELETS MEMBRANES OF
SUBJECTS RESISTANT INSULIN
Hernndez Huerta Mara Teresa
1, 2
, Prez-Campos Eduardo
1, 2
, Hernndez Cruz Pedro A
1
, Mayoral
Prez-Campos Laura
1
, Martnez Cruz Margarito
2
, Pina-Canseco Socorro
1
, Martnez Cruz Ruth
1
,
1
Laboratorio de Glicobiologa, CICIMEBIO, Facultad de Medicina y Ciruga, Universidad Autnoma
Benito Jurez de Oaxaca.
2
Unidad de Bioqumica e Inmunologa, Instituto Tecnolgico de Oaxaca.
Oaxaca de Jurez, Oaxaca. Ex hacienda de Aguilera s/n. camino a San Felipe del Agua s/n. C.P. 68020,
Tel. 9515139784. marte-hh28@hotmail.com.

Introduction. Resistance to insulin is a common foregoing type 2 diabetes and
macrovascular and microvascular abnormalities in the circulation. These complications
are associated with platelet dysfunction. Triticum vulgaris lectin (WGA) is a plant lectin
used as a tool for the isolation and characterization of many glycoconjugates, including
cell surface components. WGA recognizes the GpIb and P-selectin, but apparently the
GPIb shown to be the major protein that binds to WGA at the surface of platelets.
Methodology.Washed platelets insulin resistant subjects (80,000 platelets/ml) were

109
incubated with WGA for 10 min. And labeled with CD31 antibodies, PAC-1, CD41 and
CD62P coupled fluorochromes. The data acquisition and analysis was performed on a
flow cytometer MASCQuant (Miltenyl biotech). Results.65% of the platelet populations
of insulin-resistant subjects were positive for GPIIb/IIIa (PAC-1), when incubated with
lectin expressed 20% less, unlike the control. 10% of the population of platelets insulin-
resistant subjects expressed CD62P, when incubated with WGA increased to 15%, the
5% of healthy subjects showed expression at basal conditions but when incubated with
WGA lectin expressed 25%. For CD31 (PECAM-1) there is no difference in behavior
between healthy subjects and insulin-resistant subjects. Conclusions.Insulin-resistant
subjects membranes expressed GPIIb/IIIa and P-selectin, indicating that the cells are
activated to control difference, when incubated with WGA, the expression change
probably because the membrane is highly glycosylated of specific carbohydrate WGA
unlike control. WGA is capable of modifying the expression of GPIIb/IIIa and P-selectin
on the platelet membranes of subjects with insulin resistance.

D-04 TERMINAL GLYCOSYLATION OF IMMUNOGLOBULIN G FROM TYPE 2
DIABETES MELLITUS PATIENTS.
Candia Plata Maria del Carmen* 1, Pineda Orozco Dmaris 1, Lujn Camarillo Christian I. 1, Bolado
Martnez Enrique 1, Lpez Soto Luis Fernando 1, Gutirrez Candia Sergio 1, Soto Guzmn Jess
Adriana 1. Departamento de Medicina y Ciencias de la Salud, Universidad de Sonora 1, Hermosillo,
Sonora. Luis D. Colosio s/n, entre Reforma y Fco. Q. Salazar C.P. 83,000, Tel (662)2592121.
carmenc@guayacan.uson.mx

We assessed the terminal glycosylation of serum IgG from patients with type 2 diabetes
mellitus (T2DM) and compared it with that of the IgG from normoglycemic, age and sex-
matched, apparently healthy adults (control group). IgG was purified through a three-
step chromatographic scheme using highly acetylated-Sepharose 6B, IgA-agarose and
Protein A-Sepharose. The purity of the IgG obtained from the elution of the last
chromatographic step was confirmed by SDS-PAGE and immunoblotting. Terminal

110
glycosylation of IgG was assessed by enzyme linked-lectin assays (ELLA) using
Sambucus nigra (SNA), Maackia amurensis (MAA), Canavalia ensiformis (Con A),
Arachis hypogaea (PNA), Ricinus communis I (RCAI), Lens culinaris agglutinin (LCA),
Erythrina cristagalli agglutinin (ECA) and Triticum vulgaris (WGA) lectins. The results
obtained with single lectins assays and the lectins ratios (SNA/MAA, RCT/SNA,
RCAI/SNA, WGA/ECA, WGA/RCAI and Con A/WGA ratios) showed that the N-linked
oligosaccharides of serum IgG from patients with T2DM are both undersialylated and
undergalactosylated when compared to the control group. Abscence of terminal
galactose (including sialic acid) from the oligosaccharide chain exposes the following
carbohydrate (GlcNAc). Also, a test with LCA showed that chitobiose core of the IgG
from patients with T2DM is less fucosylated than controls. Further studies are needed to
determine the association between the state of sialylation and function of IgG in patients
with T2DM and high susceptibility to infections.

D-05 NCX1 AND NCKX1 CA
+2
EXCHANGERS IN HUMAN PLATELETS: A PUTATIVE
ROLE IN THE THROMBUS-HEMORRHAGIC EVENTS ASSOCIATED WITH
CONGENITAL DISORDER OF GLYCOSYLATION.
Bistu Milln MB
(1)
; Syravegna M
(2)
; Spcola N
(3)
; Chacn S
(4)
; Dodelson de Kremer R
(1)
; Elso de
Berberian G
(2,6)
; Asteggiano CG
(1,5,6)*
.(1)Centro de Estudio de las Metabolopatas Congnitas (CEMECO),
Facultad de Ciencias Mdicas, Universidad Nacional de Crdoba, Crdoba, Argentina. (2) Laboratorio
Biofsica, Instituto Mercedes y Martn Ferreyra (IMMF-CONICET), Crdoba, Argentina. (3)Unidad
Neurometabolismo, Servicio de Neurologa, Hospital de Nios, La Plata, Buenos Aires, Argentina. (4)
Servicio Neurologa, Gualeguaychu, Entre Ros, Argentina. (5) Facultad de Medicina, Universidad
Catlica de Crdoba, Crdoba, Argentina. (6) Consejo Nacional de Investigaciones Cientficas y
Tcnicas (CONICET), Argentina. Address: Ferroviarios 1250, Crdoba, Argentina. E-mail:
asteggianocarla@hotmail.com /web page: www.cdgargentina.com.ar

Congenital Disorders of Glycosylation (CDG) are genetic diseases due to defects in
glycoproteins or glycolipids synthesis. The phenotype is multisystemic and thrombus-
hemorrhagic events are frequently observed in these patients. In platelets, Ca
+2
signaling is

111
necessary to prevent inappropriate thrombus formation. The Na
+
/Ca
2+
(NCX) and Na
+
/K
+
-
Ca
2+
(NCKX) exchangers play a crucial role in controlling cytosolic [Ca
2+
]. The aim of this
report is to contribute to the characterization of these exchangers in human platelets, with
special emphasis in CDG patients with thrombus-hemorrhagic events. Our results
demonstrate (i) the expression of NCX and NCKX proteins in human platelets, (ii) NCX1
and NCKX1
45
Ca
2+
-uptake, (iii) heavily N- and O-glycosylated proteins by lectin-affinity
chromatography and by Western blot. Proteins were detected after the immunoprecipitation
and, with different lectins, after enzymatic deglycosilation. (iv) Lower levels of NCKX1 (n=3;
P<0.05) and reduced expression of NCX1 in a PMM2-CDG patient (n=3; P< 0.0001) and (v)
a significantly decreased Na
+
-dependent
45
Ca
+2
uptake in a PMM2-CDG patient with
abnormal platelet aggregation (t-test p< 0.05). Our results demonstrated that both
exchangers are glycosylated and that the expression and activity of NCX1 are more
dramatically reduced than NCKX1, suggesting a possible role for these exchangers, in the
abnormal platelet aggregation observed in CDG patients.
CONICETGI11220090100063, FONCyT/PICT2010-2824.

D-06 SKELETAL DYSPLASIA DUE TO CONGENITAL DISORDERS OF
GLYCOSYLATION.
EG Martinez Domenech
1
; MA Delgado
1
; P Sarrin
2
; G Matthijs
3
; N Guelbert; R Dodelson de Kremer
1
; S
Balcells
2
; D Grinberg
2
; CG Asteggiano
1,4,5
. 1 Centro de Estudio de las Metabolopatas Congntias
(CEMECO), Facultad de Ciencias Mdicas, Universidad Nacional de Crdoba, Crdoba, Argentina. 2
Depto de Gentica, Facultad de Biologa, CIBERER, IBUB, Universidad de Barcelona, Barcelona,
Espaa. 3 Center for Human Genetics, University of Leuven, Belgium. 4 Facultad de Medicina,
Universidad Catlica de Crdoba, Crdoba, Argentina. 5 Consejo Nacional de Investigaciones Cientficas
y Tcnicas (CONICET), Argentina. Address: Ferroviarios 1250, Crdoba, Argentina. Phone: 54 9 351
4586473. E-mail: asteggianocarla@hotmail.com Web page: www.cdgargentina.com.ar.

Defects in N-, O-glycosylation and combined glycosylation pathways have been identified
as Congenital Disorders of Glycosylation (CDG). Most of them are autosomal recessive, but
multiple osteochondromatosis (EXT1/EXT2-CDG) was described as a dominant disease
restricted to the cartilage. A peculiar skeletal phenotype has been described in CDG
patients and it has gained special relevance over the past few years. In patients exposed to

112
the cell hypoglycosylation due to altered glycosylation pathway, numerous extracellular
matrix proteins undergo glycosylation defects that lead to skeletal manifestations. The aim
of this work is to communicate the first CDG research program in Argentina to study the
glycobiology in skeletal dysplasia associated with CDG. Here we report the results of (i) a
cohort of 42 patients diagnosed with clinical osteochondromatosis, most of them presenting
a severe phenotype (83%), including condrosarcoma (11%), with mutations detected in
EXT1 (72%) or EXT2 (28%), (ii) a patient with osteopetrosis-like phenotype, transferring IEF
type-II and altered fucosylation observed by mass-spectrometry, in which only a
heterozygous variation was found in COG6, p.V631M (c.1891G>A); and (iii) the screening
for: GALNT3-CDG (hyperfosfatemic tumoral calcinosis); LFNG-CDG (spondylocostal
dysostosis); SLC35D1-CDG (Schneckenbecken dysplasia); B4GALT7-CDG (Progeroid-
variant Ehlers Danlos) and B3GALTL-CDG (Peter Plus Syndrome). Our results highlight the
hypoglycosylation effect on the genesis of skeletal manifestation in CDG.
FONCyT-PICT2010/2824, UCC2012, SAF2011-25431-PIB2010AR-00473.

El-01 SEROLOGICAL TESTS IMPROVEMENT FOR CHAGAS DISEASE DIAGNOSIS.
Cervantes-Landn A.Y., Martnez I., Espinoza B. Instituto de Investigaciones Biomdicas, Departamento
de inmunologa. Universidad Nacional Autnoma de Mxico. Mxico, Distrito Federal C.P. 04510. Tel.
56228944. coolaley@yahoo.com, besgu@biomedicas.unam.mx

Introduction: Chagas Disease is a parasitic infection caused by Trypanosoma cruzi and
is considered endemic of Latin America. In Mexico there are few studies about the
national prevalence. In serological tests a total protein extract is used in order to achive
good sensitivity, but cross reactions with Leishmania sp. infected individuals are also
increased. T. cruzi and Leishmania sp. share glycan structures that are often antigenic.
The objective of this work was to identify and characterize specific glycoproteins of T.
cruzi that can be use to increase specificity of serological tests for Chagas Disease
diagnosis.

GLICOBIOLOGA DE LAS ENFERMEDADES INFECCIOSAS (El)

113
Methods: Different protein fractions were obtained by molecular size exclusion
chromatography from a total extract of T. cruzi epimastigotes and immunodominant
antigens were determined by western blot. High molecular weight proteins were
extracted with detergents and sonication. The presence of antigenic glycan structures
was determined by lectin blot and these were compared to carbohydrate content in high
molecular weight proteins of L. mexicana.
Results: Proteins of 95 to 250 kDa reduced false positive results in Western blot. High
amounts of -manose, -glucose, N-acetilglucosamine and -galactose were found in
the protein fraction. Few -manose, -glucose and N-acetilgalactosamine glycans were
located in the protein fraction of L. mexicana extract.
Conclusions: High molecular weight proteins from T. cruzi decrease cross reactions
observed with Leishmaniasis patients. This fraction is rich in glycan structures in
contrast to proteins of L. mexicana. The glycan structures might be responsible for
specific antigenicity as they are being recognized by chagasic patients antibodies.

El-02 CHARACTERIZATION OF THE ADAPTIVE IMMUNE RESPONSE IN E. faecium.
Flores Moreno K. 1, Huebner J. 2, Lpez Vidal Y. 1. Programa de Inmunologa Molecular Microbiana,
Departamento de Microbiologa y Parasitologa, Facultad de Medicina, U.N.A.M. 1. University Medical
Center Freiburg, Alemania 2. Av. Universidad 3000, CP 04510; Mxico, D.F. Tel (55)56160844. E-mail:
lvidal@unam.mx

Background: Enterococcus faecium (E. faecium) is one of the main causative agents of
nosocomial infections, presents factors associated with membrane and cell wall (e.g.
glycoconjugates, capsular polysaccharide) that protecting it against phagocytosis, so an
alternative treatment with monoclonal antibodies (MAbs) against these factors could
reduce the frequency of invasive infections. Objective: Generate and characterize
monoclonal antibodies against E. faecium E155. Methodology: An inoculum of
membrane and cell wall of E. faecium E155 was immunized a group of female BALB/c.

114
The mouse with the highest antibody titer was selected and their lymphocytes were
removed and fusion with myeloma cells. The hybridomas obtained were selected with
the antibodies that enhanced reactivity against to the strain. The MAbs were
characterized by opsonophagocytc assay and the recognition to carbohydrate
structures. Results: The MAbs generated promote bacterial kill of E. faecium E155 by
opsonophagocytosis and recognition of polysaccharides structures associated to cell
wall in E. faecium E155 and cross-react with other enterococci. Conclusions: We have
MAbs against E. faecium E155 that promote opsonophagocytosis of the bacteria and
recognize polysaccharide structures in cell wall of different enterococci.

El-03 DIFFERENCES IN THE GLYCOSYLATION PROFILE OF Mycobacterium microti
RECOMBINANT STRAINS.
Viridiana Garca-Ruz 1, Patricia Ordua 1, Luis Servn-Gonzlez 2, Ral A. Agis- Jurez 1, and Yolanda
Lpez-Vidal 1. Programa de Inmunologa Molecular Microbiana, Departamento de Microbiologa y
Parasitologa, Facultad de Medicina, U.N.A.M., D.F, Mxico 1, Departamento de Biologa Molecular y
Biotecnologa, Instituto de Investigaciones Biomdicas, U.N.A.M., D.F, Mxico 2 +52 (55) 5616-0844,
Fax: +52 (55) 5616-0844, e-mail: lvidal@unam.mx.

Vaccination with Bacillus Calmette-Gurin (BCG) is the only way to prevent
tuberculosis. However, the effectiveness varies from 0-80%, this reason allows carrying
out the development of new and improved vaccines are an option recombinant
vaccines. The use of Mycobacterium microti (M. microti) was used successfully as a
vaccine in humans, M. microti is closest phylogenetically to M. tuberculosis and is not
pathogenic to humans. In this work, M. microti was recombined with antigens 85B
(Rv1886c) and PstS-1 (Rv0934) of M. tuberculosis H37Rv, to develop a vaccine
candidate against tuberculosis. M. microti have genes homologous to M. tuberculosis
H37Rv to these proteins. However we found differences in the expression of PstS-1,
which was modified by post-translational glycosylation. To identify possible
postraductional changes in proteins we searched and identified two kind of

115
glycosylation; terminal mannose and sialic acid (2-3) to galactose glycosylation with
lectin of Galanthus nivalis (GNA) and Maackia amurensis (MAA) respectively.

El-04 MODULATION OF THE SURFACE GLYCOSILATION IN HUMAN MONOCYTES
DURING DENGUE VIRUS INFECTION

Ortega-Francisco S. A
*
, Fuentes-Garca G, Garca-Pillado J, Barrios-Palacios J, Quiroz-
Garca E, Jmenez-Uribe A. P., Monroy-Martnez V and Ruiz Ordaz B.H.
Departamento de Biologa Molecular y Biotecnologa, Instituto de Investigaciones
Biomdicas UNAM. Ciudad Universitaria Coyoacan C.P. 04510 Tel: 56 22 89 31
* amyrisortega@gmail.com

Dengue is the most important vector-borne viral disease in worldwide. During Severe
Dengue, the levels of pro-inflammatory cytokines (IL-8, TNF-alpha) are increased. It is
known that pro-inflammatory cytokines in different infectious diseases modulate the
expression of cell surface glycans. Recently, it was reported the participation of
carbohydrates (glycoproteins) during dengue virus (DENV) early interactions in different
target cells.
In the present study, we evaluated the potential modulation of cell surface glycans
both, during the DENV infection of human monocytes and/or by the presence of pro-
inflammatory cytokines (IL-8 and TNF-alpha). We observed that DENV-infected
monocytes show an increase in the expression of oligosaccharides which contain sialic
acid alfa2-6. But not significant changes were present in fucoses and O-glycans.

116
However, we founded decreased levels of cell surface mannoses and alpha2-3 sialic
acid.
We observed that monocytes stimulated with TNF-alpha, increase the surface level of
sialic acid alpha2-6, while the alpha2-3 was decreased. Nevertheless, human
monocytes stimulated with IL-8, increase both conformations of sialic acids at cell
surface level.
The present data suggest the differential modulation of cell surface glycans during both,
dengue virus infection of human monocytes and pro-inflammatory cytokines.

El-05 HUMAN MILK GLYCOCONJUGATES INHIBIT HIV-1 LABORATORY STRAINS
AND PRIMARY ISOLATES IN VITRO.
Rosas-Alquicira G
1
, Acosta-Blanco I
1
, Ortega-Francisco
1
S, Fuentes-Romero
1
L, Newburg
2
D, Ruiz-
Palacios
1
G, Mao
3
Y, Zaia
3
J, Soto-Ramrez
1
L, Viveros-Rogel

M.
1
INCMNSZ,
2
Boston College,
3
Boston
University. Mexico City, Vasco de Quiroga 15, CP 14000, Tel 54870900.
moviveros@gmail.com

Background. Sulfated glycolipids (SGs) and glycosaminoglycans (GAGs) present on
colonic, vaginal and neuroglial cell bind to HIVgp120, suggesting a role in HIV infection.
We tested the ability of human milk glycoconjugates (GAGs and SGs) to inhibit in vitro
infection of HIV-1 laboratory strains and primary isolates.
Methods. Glycoconjugates were extracted from pooled human milk. HIV-1 laboratory
strains IIIB,MN and ADA-M and five primary isolates from antiretroviral-nave patients
were used on inhibition assays with MT2, THP-1 cell lines and PBMCs.
Glycoconjugates were incubated with each isolate before cell infection. HIV-1 P24
antigen was quantified by ELISA in culture supernatants. Significant inhibition of
infectivity was defined as 80% reduction of P24 with respect to controls. We also
tested non-human sulfatides and GAGs (Chondroitin sulfate). To determine HIV-1
tropism, V3 loop of gp120 was sequenced.

117
Results. GAGs significantly inhibited THP-1 infection by HIV-1ADA.

SGs fraction
inhibited MT2 infection by HIV-1IIIB and MN. Sulfatides had no inhibitory effect. GAGs
and Chondroitin sulfate inhibited infection by X4 and R5 primary isolates.
Conclusions. Human milk glycoconjugates significantly inhibited in vitro infection of HIV-
1 laboratory strains and primary isolates. Glycoconjugates from human milk are a
unique group of natural compounds that may contribute as new intervention for blocking
HIV infection.
El-06 GLYCOPROTEINS FROM A. castellanii AND ESTABLISHMENT OF INFECTION
IN NEURONAL CELLS.
Sabanero Lpez M.
1
,Soto Arredondo K.
1
, Flores Villavicencio L.L.
1
, Barbosa Sabanero G.
2
.Departamento
de Biologa
1
y Ciencias Medicas
2
, DCNyE y DCS, Universidad de Guanajuato. Noria Alta s/n, Col. Noria
Alta Guanajuato, Gto. Tel (473)7320006x8158 myrna.sabanero@gmail.com

Introduction. Acanthamoebiosis in the human causes amebic keratitis and
granulomatous encephalitis. In A. castellanii little know on surface proteins and actin
cytoskeleton during host cell interaction. In this work, we hypothesize that A. castellanii
express ligands on their surface that binds to a receptor on host cell. Moreover,
analyzed the actin filaments during parasite-host cells interaction.
Methodology. The trophozoites of A. castellanii were cultured in Chang medium with
10% fetal calf serum. Epithelial and neuronal cells were used as host cells. Interaction
assay was performed adding 10
5
trophozoites to cell monolayers at different times (15,
30 and 45 min). After, cells were exposed to antibodies against A. castellanii, secondary
antibody -Rhodamine and co-stain with Ph-FITC. The preparations were observed by
confocal microscopy. Biotin and biotinylated lectins (AGT and ConA) were used to labell
the protein and glycoprotein of trophozoite surface. To determine the proteins that could
present affinity to host cells, biotinylated proteins of A. castellanii were added to the host
cells. The bind proteins were detected by streptavidin-peroxidase.
Results and conclusion. During the infection, there are alterations of cytoskeleton of
host cells and trophozoite maintained the actin filaments in acanthopodia. A. castellanii
show surface proteins with Mr> 180-108kDa, Mr> 85-32kDa, Mr> 28-17kDa. In

118
conclusion, glycoproteins with MW > 130, 90 and 54 kD show affinity to epithelial and
neuronal cells, initiate the adhesion process and cell invasion. We are currently working
to identify these molecules and characterize signal mechanism that regulated the
invasion.

Acknowledgments. Work in our laboratory is supported by grants from the CONACYT 157577 and DAIP,
UG-2011-MSL.

El-07 ANALYSIS OF THE OCH1 GENE FAMILY IN Sporothrix schenckii.
Carmona-Baca Karla A.
1,2
, Lozoya-Prez Nancy E.
1
, Lpez-Ramrez Luz A.
1
, Mora-Montes Hctor M
1,
*.
1
Departamento de Biologa, Universidad de Guanajuato, Noria Alta s/n, Col. Noria Alta, C.P. 36050,
Guanajuato, Gto., Mxico. Tel. 473 732 0006, ext. 8154.
2
Instituto Tecnolgico de Morelia, Av.
Tecnolgico 1500, Col. Lomas de Santiaguito, C.P. 58120, Morelia, Michoacn, Mxico. Tel 443 312
1570, ext 211.
*E-mail: hmora@ugto.mx

S. schenckii is a dimorphic fungal pathogen and the causative agent of sporotrichosis, a
subcutaneous infection that affects humans and other mammals. Thus far, little is
known about basic aspects of its biology, including its cell wall composition and
biosynthesis. The S. schenckii cell wall is composed of 1,3-, 1,6- and 1,4-glucans.
Furthermore it also contains rhamnomannan and rhamnogalactan, which are
oligosaccharides attached to cell wall proteins during the glycosylation pathways. These
biosynthetic routes are poorly studied in S. schenckii, but have been thoroughly revised
in other fungal systems such as Saccharomyces cerevisiae and Candida albicans. The
-mannosyltransferase Och1 is a Golgi-resident enzyme involved in the elaboration
of N-linked glycans and has a key role in the extension of the N-linked outer branch of
S. cerevisiae and C. albicans. However, in molds such as Aspergillus there are OCH1
duplications and the genome contains three OCH1-like genes that are grouped in the
OCH1 gene family. The role of these proteins in the glycosylation pathways is currently
unknown. Here, using bioinformatics tools we have indentified the members of the
S. schenckii OCH1 gene family and have isolated their cDNAs, confirming they are

119
functional genes. We are currently heterologously expressing the genes in Pichia
pastoris to determine their enzyme activity, and performing complementation assays in
C. albicans and S. cerevisiae och1
functional orthologs of Och1.


El-08 ADENOVIRUS HUMANO AD5 MODIFICA LA FUCOSILACIN CELULAR EN UN
MODELO IN VITRO DE EPITELIO PULMONAR HUMANO.
Gutirrez Huante Kathya 1, Gonzlez Garca C. Ramn 2, Martnez Duncker R. Ivn 1. 1 Laboratorio de
Glicobiologa Humana, 2 Laboratorio de Virologa molecular. Facultad de Ciencias, UAEM. Av.
Universidad 1001. Col. Chamilpa, Cuernavaca, Mor. 62209. Tel (777)3297000 ext. 3664
duncker@uaem.mx

Introduccin: Algunas infecciones virales (ej. CMV, VZV, HSV-1 y HTLV-1) aumentan la
expresin de fucosiltransferasas (FUTs) de la clula infectada induciendo la sntesis de
antgenos de la familia Lewis (ej. sLe
x
, Le
y
), lo que se ha sugerido influye en el proceso
de la enfermedad. El objetivo de este trabajo fue determinar si la infeccin con
Adenovirus 5 (Ad5), un virus patgeno pulmonar humano, induce cambios en la
fucosilacin celular, para ello se utiliz la lnea celular A549 como modelo de epitelio
pulmonar humano. Metodologa: Clulas A549 fueron infectadas con Ad5 y su perfil de
fucosilacin analizado a las 8, 16 y 24 h.p.i. por citometra de flujo con las lectinas AAL
(ncleo 1-6 fucosa y 1-2/3 fucosa terminal), LCA (ncleo 1-6 fucosa) y UEA-I (1-2
fucosa terminal). Resultados: Las clulas infectadas presentaron un aumento de IMF
estadsticamente significativo (p<0.05) en la unin de UEA-I en todos los tiempos
estudiados. Se analiz por qPCR a las 24 h.p.i. la expresin de los genes que codifican
para las 1-2 fucosiltransferasas FUT1 y FUT2. Slo el gen FUT2 mostr un aumento
estadsticamente significativo en clulas infectadas (2.3 folds), respecto a clulas
control. La expresin del antgeno 1-2 fucosilado Le
b
como posible candidato de unin

120
de UEA-I fue descartado en vista de que las clulas resultaron negativas para dicho
antgeno. Conclusiones: La infeccin con Ad5 aumenta la expresin de estructuras 1-
2 fucosiladas a expensas de estructuras distintas a Le
b
(ej. Le
y
) y est asociado a un
aumento en la expresin del gen FUT2.

EI-09 A MANNOSE RICH GLYCOCONJUGATE PRESENT IN TROPHOZOITES
FROM E. histolytica JUST RECOVERED FROM EXPERIMENTAL LIVER
ABSCESSES.
Fernndez Gonzlez C.D.
1
, Nequiz Avendao M.
1
, Gonzlez Canto A.
1
, Ramos Martnez E.
1
, Lpez
Vancell R.
1
& Perez Tamayo R.
1
. Depto. Medicina Experimental, Fac. Med.UNAM. Hosp. Gral. de
Mxico, Mxico D.F. Dr. Balmis148. C.P. 06726, Tel. 56232664. lvsario@gmail.com .

Entamoeba histolytica is the protozoan parasite responsible of amebiasis in humans.
The molecular mechanisms that regulate parasite invasion and tissue damage are not
well characterized. Different species and strains of Entamoeba exhibit various levels of
pathogenicity. The strain most commonly used in experimental research is HM1:IMSS,
which is referred as the virulent one because of its ability to induce the formation of
liver abscesses in experimental animals. Although being virulent, this condition is lost
when axenic culture is sustained for long periods, so to maintain it, trophozoites
axenically cultured must be passed through hamster livers twice a month, recovering
the parasites from the abscesses produced to restart the axenic culture. The existence
of the two variants: virulent and non-virulent from the same strain has allowed us to
compare the expression and localization of the Gal/GalNAc lectin, as well as the
glycosylated pattern of this lectin in trophozoites from one or another variants. In this
work, we have analyzed the glycoconjugates present in trophozoites from E. histolytica
HM1:IMSS just recovered (cultured for two days after dissected) from liver abscesses
and found a glycoconjugate rich in mannose, with an approximate molecular weight of
150 kDa which is not present in virulent or non virulent trophozoites. We have isolated it
by Galanthus nivalis affinity chromatography and are currently trying to identify it.

121

El-10 SEQUENCE SIMILARITY OF THE ENZYMES INVOLVED IN THE
BIOSYNTHESIS OF CAPSULAR POLYSACCHARIDES IN TWO MEDICAL
IMPORTANT BACTERIA AS POSSIBLE RESPONSIBLE FOR THE PRESENCE OF
CROSSED IMMUNITY. Solano Rodrguez Luciana 1,Mndez Capdeville Teresita
Mildred 2. Research Dept,1. Production Dept.,BIRMEX 2. Mxico DF. Mariano
Escobedo 20 C.P. 11400, Tel 50820390 ext. 3334. solano0103@yahoo.com.mx .

Introduction.It has been reported the presence of crossed-immunity between of E. coli
K1 and Hib polysaccharides, because between the chemical structures it is not possible
to perform an alignment to find some degree of similarity, was worked with the protein
sequences involved in the polysaccharides production for each bacteria.Methodology.
Sequences were analyzed of the proteins involved in the synthesis of capsular
polysaccharide for each bacteria; these belong to region 2 of the genetic organization of
the gene cluster of capsule E. coli K1 and Hib. The protein sequences of ORF1 and
ORF2 of Hib were aligned against the protein sequence NeuD, NeuB, NeuA, NeuC,
NeuA, NeuS of E. coli K1.The sequences were carried to their FASTA format and
aligned using Geneious 5.5.4 software.Results. Was obtained a small percentage of
similarity between 15 to 19% the above is confirmed by comparing the protein
sequences of ORF 1 and ORF4 in the BLAST and not finding similarity to E. coli
K1.Conclusions.The similarity found among proteins participating in the biosynthesis of
the polysaccharides of E. coli K1 and Hib is very small (15-19%). We suggest that the
presence of crossed immunity between E. coli K1 and Hib is not given by these proteins
of region 2. The reported crossed immunity for these bacteria is mainly given by the
similarity of other proteins (70%) of regions 1 and 3.

C-01 SELECTIVE ANTICANCER ACTIVITY OF SPIROSTANIC GLYCOCONJUGATES
Fernndez-Herrera Mara Antonieta,
1
Sandoval-Ramrez Jess,
1
Snchez-Snchez Luis,
2
Ramrez-
Gutirrez Ramss Elas,
1
Galicia-Morales Isaas,
1
Escobar-Snchez Ma. Luisa,
3
Gonzlez-Ballesteros
Mauricio M.,
2
Lpez-Muoz Hugo.
2

1
Facultad de Ciencias Qumicas, BUAP. Puebla, Pue. Ciudad Universitaria C. P. 72570, Tel. 01-222-229-
55-00 Ext. 2842, Mexico.
2
Facultad de Estudios Superiores-Zaragoza, UNAM. Mexico City, Mexico.
3
Facultad de Ciencias, UNAM. Mexico City, Mexico.
marieta.fernandez@correo.buap.mx

GLICOBIOLOGA DEL CNCER (C)

122
Steroidal glycoconjugates are complex amphiphilic glycosides which display
diverse biological activities including antitumor activity. Such compounds are usually
divided into cholestanic, furostanic, and spirostanic (Figure 1); all of them are
biosynthetically related. Recently, intensive research has been focused on developing
saponins for tumor therapies. Diosgenyl glycosides are the most common spirostanic
saponins in plants and it has been reported that exhibit potent antiproliferative activity in
several human cancer cells.
We have previously
reported the selective
anticancer activity of
new synthetic saponins.

In this work, we describe structural and biological insights of a series of
monodesmosidic diosgenyl saponins containing one, two or three sugar moieties
attached at C-3 (Figure 2). The antiproliferative activity was evaluated on cervicouterine
cancer cells in in vitro assays, as well as the effect of this series of spirostanic
glycoconjugates on non-tumor cells (peripheral blood lymphocytes) We observed a
correlation between the nature of the saccharide and bioactivity.
In order to evaluate specific functional
groups as key pharmacophoric structural
features we performed and analyzed the
MEPs on the molecular surfaces applying
the Fukui function. The MEPs showed the
most susceptible points for the title compounds, and
for the aglycon.

These in vitro and in silico evaluations,
certainly augur well for deeper assays on
mechanistic effects in the next stage of the
Figura 3. Molecular Electrostatic Potential (MEP) of the
aglycon diosgenin, calculated with B3LYP/6-31+G**
(isosurface -0.01,0.08).

123
research. We believe, therefore, that these compounds serve as promising lead
candidates for further biological and theoretical screening.
Data about extraction, synthesis and spectroscopy will be presented; as well as
the biological and in silico studies.

C-02 ARACHIDONIC ACID INDUCES AN INCREASE OF -1,4-
GALACTOSYLTRANSFERASE I EXPRESSION IN MDA-MB-231 BREAST CANCER
CELLS.
Villegas Comonfort S
1
, Navarro Tito N
2
, Serna Marquez N
1
, Galindo Hernandez O
1
, Prez Salazar E
1*
.
Departamento de Biologa Celular. Cinvestav-IPN. Av. IPN # 2508. San Pedro Zacatenco. Mexico, DF.
07360. Mxico y
2
Laboratorio de Biologa Celular del Cncer UACQB-UAGro, Guerrero, Mxico.
*Corresponding author. e-mail address: jperez@cell.cinvestav.mx

Background. Arachidonic acid (AA) and its metabolites are implicated in FAK activation
and cell migration in MDA-MB-231 breast cancer cells, and an epithelial-to-
mesenchymal-like transition process in mammary non-tumorigenic epithelial cells
MCF10A. During malignant transformation an altered expression of glycosiltransferases
is present, which promotes changes on the glycosilation leves of cell-surface proteins.
The -1,4-galactosyltransferase I (GalT I) is an enzyme localized in two distinct
subcellular compartments, the trans-Golgi apparatus and the cell surface. In the trans-
Golgi apparatus, it performs biosynthetic functions, while on the cell surface participates
like a receptor for extracellular matrix proteins, mediating a variety of biological
functions, including cell growth, migration and spreading. However, the participation of
AA in the regulation of GalT I expression and the role of this enzyme in the cell
adhesion process in breast cancer cells remains to be investigated. Methodology By
using western blot, flow cytometry and cell adhesion assays, we analyzed the
expression of GalT I. Results and conclusions We demonstrate that AA induces an
increase of GalT I expression through a PLA2, Src, ERK1/2 and LOXs activities-
dependent pathway in MDA-MB-231 breast cancer cells. Moreover, MDA-MB-231 cells

124
adhere to laminin via GalT I expression and pretreatment of cells with AA induces and
increase of cell adhesion to laminin. In conclusion, our findings demonstrate, that AA
promotes GalT I expression through an AA metabolism, Src and ERK1/2 activities-
dependent pathway, and that GalT I plays a pivotal role in cell adhesion to laminin in
MDA-MB-231 breast cancer cells.

C-03 STUDY OF LECTIN BINDING PATTERN IN LUNG CANCER CELLS TREATED
WITH (-)-EPICATECHIN.
Njera Garca Nayelli, Varela Castillo Omar, Rubio-Gayosso Ivan, Palma Lara Icela,
Gutirrez Iglesias Gisela, Ceballos-Reyes Guillermo. Laboratorio de Investigacin
Integral Cardiometablica, Seccin de estudios de Posgrado en Investigacin. Escuela
Superior de Medicina, IPN. Plan de San Luis y Salvador Diaz Mirn s/n. Mxico, D.F.
C.P. 11340. Tel. 57296300 Ext. 62820. gceballosr@ipn.mx

In Mexico malignant tumors from the trachea, bronchus and lungs are neoplasias
leading cause of death, smoking associates in 90% of cases. Treatment depends on
several factors such as; cell lineage, size, location and spread of the tumor. Usually,
combination of therapies such as surgery, radiotherapy and chemotherapy are
employed for their treatment. Chemotherapy is indicated in advanced stages (IIIB and
IV) and cisplatin-based regimens are commonly used however, adverse toxicological
effects are frequent. Flavonoids are molecules that shown anti-tumoral and
cardioprotective effects. We explored the possibility of use flavonoids as adjuvants in
cisplatin-based cancer treatment. Cisplatin therapy combined with (-)-epicatechin, the
most abundant flavanol in cacao and green tea showed a synergistic effect on the
inhibition of proliferation of lung cancer cells in culture. The mechanism through (-)-
epicatechin increases the effect of cisplatin remains unknown. Tumor cell glycosilation
has an important role in growth, invasion and metastasis. In this work, we present
evidence demonstrating that (-)-epicatechin modifies the lectin binding pattern in lung

125
cancer cells in culture, showing the existence of increased fucose and mannose
residues in plasmmalema surface proteins or lipids. This increase would lead to
activation of the lectin pathway, promoting the recognition by specific binding proteins
such as the Mannose-Binding-Lectin (MBL). This process might translate in vivo, to a
higher possibility of specialized cells to recognize cancer cells in the process of
apoptosis to be removed more efficiently from the tissue.

C-04 EXPRESSION OF SIALIC ACID AND GALECTIN-3 IN MCF-7 CELLS
STIMULATED WITH TNF
Acevedo Prez Miguel ngel
1
Vsquez Aquino Marisol
1
, Hernndez Cruz P
1
, Prez Campos E
1
, Pina
Canseco MS
1
y Gallegos Velasco B*
1
.
1
Laboratorio de Glicobiologia CICIMEBIO, Facultad de Medicina y Ciruga, Universidad Autnoma Benito
Jurez de Oaxaca.
2
*ITANBEL@hotmail.com

INTRODUCTION: infiltrating ductal carcinoma is the most common in women who have
some alteration in the breast tissue. It has been found that in the majority of the cells of
carcinomas there is an incomplete of the elongation oligosaccharide chains, forming
structures less complex as the antigens T (Gal 1,3GalNAc1-O-Ser/Thr) and Tn
(GalNAc1-O-Ser/Thr), which are prematurely linked with acid sialic, these antigens are
associated with the uncontrolled growth of cells and the evasion of the immune system
by the cells which express. These antigens are expressed in various, type mucin
glycoproteins present in the membranes of MCF-7 cells and may be responsible for the
accession and cellular transformation. OBJECTIVE: Study the expression of sialic acid
and galectin-3 in cells MCF-7, stimulated with TNF . METHODOLOGY: MCF-7 cells
are grown in RPMI-1640 supplemented with 10% inactivated fetal bovine serum, 100
U/ml penicillin, 100 g/ml streptomycin, and 2 mm L-glutamine and incubated at 37 C
with 5% CO
2
. Cytochemistry 300.000 cells are deposited on a plate with six wells and

126
grown during 24; plates are washed three times with PBS. The plates were incubated
with the lectins at concentration of 5 g/ml during 24 hours, and/or antigalectina-3
during 1 hour, after; the plates were washed with PBS and incubated for 30 minutes
with streptavidin-peroxidase prepared 1 to 1000 on PBS. Washed 3 times with PBS and
add the substrate 3-amino-9-ethyl-carbazol. Some tests were performed with TNF
stimulation at a concentration of 5 g/ml. Double labeling of slides were performed as
follows: Plates were labeled with lectins (1g/ml) overnight at 4C and monoclonal anti
galectin-3 only that lectin binding was indirectly recognized with streptavidin-FITC
conjugated and visualized using green filter. Anti Galectin antibodies were revealed with
streptavidin-Red-X conjugate. Plates were observed with AXIOSCOP 40 microscope
equipped with digital camera AXIOCAM MRC and micrographs analysed with ZEN pro
2011 Software. Some experiments were carried out for flow cytometry using a flow
cytometer (Macs QuantMR MiltenyiBiotec) and data were analyzed with the software of
cytometry MACSQuantify version 2.4 RESULTS: There is an expression of sialic acid in
the cells MCF-7, which recognized by the lectins from Maackia amurensis and
Sambucus nigra, also, there was expression of galectin-3, the expression of the sialic
acid decreases when the cells are stimulated with TNF CONCLUSIONS: The
expression of the sialic acid decreases when the cells are stimulated with TNF.

PROJECT FUNDED BY PROMEP UABJO-PTC-060

C-05 PROTEOMIC IDENTIFICATION OF DIFFERENT PROFILES OF FUCOSYLATED
HAPTOGLOBIN ISOFORMS IN ASCITIC FLUID FROM OVARIAN
CYSTADENOCARCINOMA MEXICAN PATIENTS. CORRELATION WITH ADVANCED
CLINICAL STAGES.
Garibay-Cerdenares OL
1
, Osorio-Trujillo JC
1
, Hernndez-Ramrez VI
1
, Hernndez-Ortz M
3
, Gallardo-
Rincn D
2
, Cant de Len D
2
, Encarnacin-Guevara S
3
and Talams-Rohana P
1
.
1
CINVESTAV-IPN.
Depto.IPM. Mxico D.F. Av. IPN 2508, Col. San Pedro Zacatenco, Del. Gustavo A. Madero, Cdigo
Postal 07360. Apartado Postal: 14-740, 07000 Mxico, D.F. Tel: +52 (55) 5747 3800. Ext. 5636.

127
ptr@cinvestav.mx
2
INCan. Mxico, D.F.. Av. San Fernando No. 22, Col. Seccin XVI Delegacin Tlalpan,
C.P. 14080.
3
CCG, UNAM, Cuernavaca, Morelos. Universidad 565 Chamilpa, 62214.
Abstract
Ovarian cystadenocarcinoma is the most lethal gynecologic malignancy worldwide.
Over 70% of patients present accumulation of peritoneal fluid or ascites as a sign
associated with the final stage of the disease. Ascites are a rich source of growth factor
activity; tumoral cells have the ability to survive in the ascites while lacking immediate
proximity to vasculature.
The project overall goal was to perform a proteomic profile of ascitic fluid from patients
with ovarian cancer to identify proteins with differential expression profiles. Once
identified, to determine the probable function of this selected protein.
Samples (50) were collected from ovarian cancer diagnosed patients who have not
undergone chemotherapy treatment.
2D-electrophoresis using IPG strips pH 5-8. More than 400 spots were resolved, and an
area of nine spots with differential expression pattern among samples was selected.
Mass Spectrometry identification of these 9 spots showed 7 Haptoglobin isoforms and 2
isoforms of Transthyretin. Densitometric analysis established that Transthyretin
isoforms were constitutively expressed in all samples while clear differences in the
expression pattern of Haptoglobin were found. In addition, the fucosylated state of
Haptoglobin isoforms in 19 samples was analyzed by Aleuria aurantia Lectin binding by
1D Western blot assays. Both Haptoglobin isoforms number and its fucosylated state
showed a strong correlation with advanced clinical staging of patients.
Based on the above results, we suggest that fucosylated-Hpt could become an ovarian
cancer progression biomarker. This hypothesis needs to be validated in a future
prospective project.

128

C-06 TUMOR ANTIGEN SLE (X) EXPRESSION IN CERVICAL SCRAPES WITH
RECURRING SQUAMOUS INTRAEPITHELIAL LESION.
Hernndez-Pacheco Raquel Esneidy
1
, Garca-Lpez Roco Berenice, Almazo-Domnguez Roco
2
, Flores-
Mendoza Lilian
1
, Santos-Lpez Gerardo
1
, Reyes-Leyva Julio
1
, Vallejo-Ruiz Vernica
1
. Centro de
Investigacin Biomdica de Oriente, IMSS
1
Hospital General de Zona No. 5 Metepec
2

veronica_vallejo@yahoo.com

INTRODUCTION. During malignant transformation, changes occur at the level of
cellular glycosylation which modify cell-cell and cell-extracellular matrix. Several studies
in patients with cervical carcinoma have reported increases in sialic acid levels in
cervical tissue, blood and urine. Other studies report that sialic acid levels decrease in
patients who respond to therapy. The increased expression of various sialylated
antigens have been reported in cancer. The sLe (x) antigen is increased in malignant
cervical as well as premalignant lesions. MATERIALS AND METHODS. Cervical
scrapings were obtained from healthy patients with normal cytological diagnosis and
from patients diagnosed with squamous intraepithelial lesion (LEI) who were treated by
loop electrosurgical excision procedure and some of which had recurrent LEI. The
samples were processed to determine the presence of tumor antigen sLe (x) using the
technique of flow cytometry using the antibody anti-sLe (x) coupled to phycoerythrin.
RESULTS. The total number of samples collected was 29. Twelve of them form the
group of women with normal cytology, nine samples were processed for groups without
recurrence of injury and eight for the group with recurrence. Preliminary results from
cytometric analysis showed a higher percentage of antigen-positive cells sLe (x) in the
samples of patients with recurrent LEI. CONCLUSIONS. The expression of sLe (x) was
higher in samples with recurrent LEI.

129

C-07 SINGLE NUCLEOTIDE POLYMORPHISMS IN THE B3 PROMOTER OF ST3GAL 4
GENE IN PATIENTS WITH CERVICAL SQUAMOUS INTRAEPITHELIAL LESIONS AND
CERVICAL CANCER
Rivera-Jurez M.A.
1,2
, Rosas-Murrieta N.H.
2
, Mendieta-Carmona V.
1
, Hernndez-Pacheco R.E.
4
, Rodea-
vila C.
3
, Apresa-Garca T.
3
, Jave-Suarez L.F.
5
, Aguilar-Lemarroy A.
5
, Ceja-Utrera F.J.
6
, Vzquez-
Zamora V.J.
6
, Reyes-Salinas J.S.
6
, Vargas-Maldonado M.T.
6
, Daz y Orea A.
4
, Zamora-Guinez I.
4
, Sosa-
Jurado F.
1
, Santos-Lpez G.
1
, Reyes-Leyva

J.R.
1
, and Vallejo-Ruiz V.
1
1 Laboratorio de Biologa Molecular, Centro de Investigacin Biomdica de
Oriente, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Federal Atlixco-Metepec, Atlixco, Puebla,
Mxico C.P. 74360
2 Laboratorio de Bioqumica del Instituto de Ciencias y Posgrado en Ciencias Qumicas, Universidad
Autnoma de Puebla, Puebla, Puebla, Mxico. Ap.Postal 1613, 72000
3 Hospital de Oncologa, Centro Mdico Nacional Siglo XXI, IMSS, D.F., Mxico.
4 Facultad de Medicina, Universidad Autnoma de Puebla, Puebla, Puebla, Mxico.
5 Centro de Investigacin biomdica de Occidente, IMSS, Guadalajara, Jalisco, Mxico.
6 Hospital de Especialidades Manuel vila Camacho, IMSS, Puebla, Puebla, Mxico.
Email, veronica_vallejo@yahoo.com)

Abstract
Cervical cancer is a multifactorial disease, one of the features of cancer changes are
alterations in the glycosylation. During malignant transformation changes occur in the
expression of mucin type mainly oligosaccharides and sialic acids. The levels of sialic acid
(SA) on the cell surface are associated with the levels of messenger RNA (mRNA) of
certain genes of the sialyltransferases (STs). The mRNA levels of the ST3Gal III, ST6Gal I
and ST3Gal IV is altered during malignant transformation suggesting that these enzymes
are strongly implicated in the development and progression to cervical cancer.

They have
identified single nucleotide polymorphisms (SNPs) associated with the risk of developing
cervical cancer. The purpose of our study was to investigate the association of
polymorphisms (SNPs) in the B3 promoter of st3gal 4 gene with the presence of pre-
malignant cervical lesions and cervical cancer, as well as the activity of the promoters. The
study included 154 patients with cervical lesions, 100 patients with cervical cancer and 100

130
women with normal cervix. The alleles of the SNPs were analyzed by direct sequencing of
PCR products. The SNP located at position -22 (rs10893506) is significantly associated with
the risk for developing cervical intraepithelial neoplasia and cervical carcinoma. Both
promoters with different alleles (SPN rs10893506) were cloned in the reporter vector
pGL4.12. Luminescence assays were used to determine the promoter activity. The
promoter with the C allele of SNP rs10893506 associated with the risk for developing
cervical lesions and / or cervical cancer showed greater activity than promoter with allele T.

POLIMORFISMOS DE UN SOLO NUCLETIDO EN EL PROMOTOR B3 DEL GEN
ST3GAL 4 EN PACIENTES CON LESIN ESCAMOSA INTRAEPITELIAL CERVICAL Y
CNCER CERVICOUTERINO.
Rivera-Jurez M.A.
1,2
, Rosas-Murrieta N.H.
2
, Mendieta-Carmona V.
1
, Hernndez-Pacheco R.E.
4
, Rodea-
vila C.
3
, Apresa-Garca T.
3
, Jave-Suarez L.F.
5
, Aguilar-Lemarroy A.
5
, Ceja-Utrera F.J.
6
, Vzquez-
Zamora V.J.
6
, Reyes-Salinas J.S.
6
, Vargas-Maldonado M.T.
6
, Daz y Orea A.
4
, Zamora-Guinez I.
4
, Sosa-
Jurado F.
1
, Santos-Lpez G.
1
, Reyes-Leyva

J.R.
1
, and Vallejo-Ruiz V.
1.
Email,
veronica_vallejo@yahoo.com)
1 Laboratorio de Biologa Molecular, Centro de Investigacin Biomdica de Oriente, Instituto Mexicano
del Seguro Social, Km 4.5 Carretera Federal Atlixco-Metepec, Atlixco, Puebla, Mxico C.P. 74360
2 Laboratorio de Bioqumica del Instituto de Ciencias y Posgrado en Ciencias Qumicas, Universidad
Autnoma de Puebla, Puebla, Puebla, Mxico. Ap.Postal 1613, 72000
3 Hospital de Oncologa, Centro Mdico Nacional Siglo XXI, IMSS, D.F., Mxico.
4 Facultad de Medicina, Universidad Autnoma de Puebla, Puebla, Puebla, Mxico.
5 Centro de Investigacin biomdica de Occidente, IMSS, Guadalajara, Jalisco, Mxico.
6 Hospital de Especialidades Manuel vila Camacho, IMSS, Puebla, Puebla, Mxico.

Resumen
El cncer cervicouterino (CaCu) es una enfermedad multifactorial, uno de los cambios
caractersticos del cncer son las alteraciones a nivel de la glicosilacin. Durante la
transformacin maligna ocurren cambios en la expresin de oligosacridos
principalmente tipo mucina

y cidos silicos.

Los niveles de cido silico (AS) en la
superficie celular se han asociado con los niveles de ARN mensajero (ARNm) de los
genes de ciertas sialiltransferasas (STs). Los niveles de ARNm de la ST3Gal III,

131
ST3Gal IV y ST6Gal I se alteran durante el proceso de transformacin maligna.

Se han
identificado polimorfismos de un slo nucletido (SNPs) vinculados con el riesgo para
desarrollar CaCu. El propsito de nuestro estudio fue investigar la asociacin de
polimorfismos (SNPs) dentro del promotor B3 del gen st3gal 4 en pacientes con
lesiones premalignas de crvix y CaCu, as como evaluar la actividad de los
promotores. En el estudio se incluyeron 154 pacientes con lesiones premalignas, 100
pacientes con CaCu y 100 mujeres sin alteracin citolgica. Los alelos de los SNPs
fueron identificados por secuenciacin directa de los productos de PCR. El SNP
localizado en la posicin -22 (rs10893506) est asociado de manera significativa con
el riesgo de desarrollar lesiones premalignas y/o CaCu. Se clonaron ambos alelos del
SPN rs10893506 en el vector reportero PGL4.12 y a travs de ensayos de
luminiscencia se determin la actividad del promotor. El promotor B3 con el alelo C del
SNP rs10893506 asociado con el riesgo para desarrollar lesiones cervicales y/o CaCu
mostr mayor actividad que el promotor con el alelo T.

T-01 RELEVANCE OF O-ACETYL AND PHOSPHOGLYCEROL GROUPS FOR THE
ANTIGENICITY OF Streptococcus pneumoniae SEROTYPE 18C CAPSULAR
POLYSACCHARIDE.
Chang Caldern J.; Serrano Rodrguez Y.; Garrido Artega R.; Rodrguez Noda L. M.;
Pedroso Fernndez J.; Cardoso San Jorge F.; Valds Balbn Y.; Garca Rivero D.;
Fernndez Santana V.
*
; Vrez Bencomo V. janoi.chang@cqb.cu
Center for Biomolecular Chemistry, Playa, Havana, Cuba.

Capsular polysaccharides are important virulence factors of Streptococcus pneumoniae.
The polysaccharide has been used as a component of vaccines against pneumococcal
GLOCOBIOTECNOLOGA (T)

132
diseases either as plain polysaccharide or better conjugated to a protein. The last one is
the vaccine of choice to target child protection. The immune responses depend on
several polysaccharide physicochemical properties that can be affected during either
purification or modification in the case of conjugate vaccines. In serotype 18C, the
repeating unit has a complex structure having a branched pentasaccharide with two
apparently labile subtituents: glycerol-phosphate and O-acetyl group. The loss of these
groups may potentially reduce the ability of the 18C polysaccharide to induce the
desired immune response. Therefore, the relationship of both groups with the
antigenicity and immunogenicity of 18C capsular polysaccharide is explored. It is shown
that glycerol-phosphate must be preserved for conserving adequate antigenicity of the
18C capsular polysaccharide. At the same time, it was proved that O-acetyl groups do
not play any role for the antigenicity and immunogenicity

* Dedicated to her memory. Deceased 20
th
November 2011

T-02 LECTIN OF CORN COLEOPTILE BEHAVIOR AS A RESPONSE AGAINST
Aspergillus parasiticus IN VIVO.
Snchez-Medina M.A.
1
, Isidro-Lzaro J.
1
, Pina-Canseco M.S.
2
, Prez-Santiago A.D.
1

1
Instituto
Tecnolgico de Oaxaca, Av. Vctor Bravo Ahuja No. 125 Esq. Calz. Tecnolgico, Oaxaca, Oax. C.P.
68030.
2
Universidad Autnoma Benito Jurez de Oaxaca, Carretera Antigua a San Felipe del Agua S/N.
Oaxaca, Oax. C.P. 6820. mmedinaito@gmail.com

Aspergillus parasticus is a fungal pathogen that produces aflatoxins in cereals such as
maize, where it finds ideal parameters for its development. Due to the damages
suffered by this grain, studies have been carried out for looking for metabolites which
confer it resistance against this fungus. A previous study shows that plant lectins can
mediate pathogen recognition; these proteins play an important role within the plant. In
order to determine if there is a variation in corn coleoptile lectin inoculated with A.
parasiticus, the behavior of two maize samples was evaluated: one of them resistant to

133
aflatoxin production, and the other, susceptible. The lectin activity of protein was
monitored every 24 hours in healthy and infected seedlings. In kinetics for the resistant
sample, it was determined that the lectin activity and protein in infected plant have a
higher concentration than in healthy plant. The susceptible maize sample showed
higher activity in healthy plant. The UV microscopic analysis showed large
agglomerates of cells indicating a strong interaction between fungus-LCM in the
resistant maize sample. The results show that the lectin activation is expressed in
greater concentration in the resistant sample than in the susceptible one.

T-03 ANTHOCYANIN CONTENT, PHENOLIC COMPOUNDS AND ANTIOXIDANT
ACTIVITY IN BLACKBERRY FRUIT (Rubus fructicosus) AND JAM FROM ESTADO DE
MXICO.
Luna Ramrez, Karem Yazmin.
1
; Arellano Crdenas, Sofa.
1
; Cornejo Mazn, Maribel.
1
1
Instituto Politcnico Nacional. Escuela Nacional de Ciencias Biolgicas. Departamento de Biofsica
Prolongacin de Carpio y Plan de Ayala s/n Col. Santo Toms. C.P. 11340. Mxico, D.F. Tel:
015557296300. Ext: 62314
ky_luna@yahoo.com; sofiare@hotmail.com; maribelpabe2@hotmail.com

In the last years great emphasis has given to antioxidants present in fruit and vegetables,
especially in natural pigments as anthocyanins which had been attributed the capacity to reduced
coronary heart disease, chronic disease such as cancer, hypertension, cataract and diabetes type
2. Blackberry is an excellent source of antioxidants especially in anthocyanins where the
cyanidine-3-glucoside (C3G) is highlight. It is demonstrated that there is a reduction in
antioxidant activity during the processing and storage of fruits that it may decrease many of the
health beneficial effects of these products, for example: jams.
In this work, physicochemical characterization of fresh fruit of blackberry has been determined
(humidity, pH, total soluble solids, acidity, density and reducing sugars) and also performing an
analysis of the product components in a jam prepared and stored at 4 C for three months.

134
As well to both fruit and jam, monomeric anthocyanin quantification has been performed by pH-
differential method thus obtaining 2273.55 31.1 and 592.92 3.16 mg eq C3G/kg FW for
fresh fruit and jam respectively, at the end of the jam analysis were obtained 439.18 1.2 mg eq
C3G/kg FW. The phenolic compound content (by the Folin-Ciocalteu assay), antioxidant activity
(by DPPH assay) and, ascorbic acid were also determined in this research, all determinations by
triplicate.
Although the samples were in a cool constant temperature, we could watch degradation in the
principal compounds that give antioxidant activity.

T-04 PRODUCTION AND PARCIAL PURIFICATION OF FUCOSYL-
OLIGOSACCHARIDES BY TRANSGALACTOSILATION REACTION
Escamilla-Lozano Yolanda
1
, Sosa-Ancona Erik
1
, Garca-Garibay Mariano
1,2
, Gomez-Ruiz Lorena
1
,
Rodrguez-Serrano Gabriela
1
, and Cruz-Guerrero Alma
1
,
1
Dpto. de Biotecnologa, Universidad Autnoma
Metropolitana-Iztapalapa, CP 09340,
2
Dpto.de Ciencias de la Alimentacin Universidad Autnoma
Metropolitana-Lerma. CP 52006.

email: yescamilla_lozano@hotmail.com

Introduction. Synthesis of oligosaccharides is currently receiving a great deal of
attention due to the important role of these compounds in many biological processes.
The use of glycosylhydrolases in the synthesis of oligosaccharides is an attractive way
1
.
There is a growing interest in the availability of human milk oligosaccharides, especially
fucosyl-oligosaccharides, to protect infants from enteric pathogens during early
development
2
. The aim of this study was to synthesize a fucosyl-oligosaccharide by
transgalactosilation reaction with galactosidase from Aspergillus oryzae.
Methodology. The enzymatic reaction was performed at 60 C with constant agitation at
200 rpm at pH 4.5, for 12 h, using lactose as donor and fucose as acceptor. -
galactosidase from Aspergillus oryzae was used. The synthesis of the fucosyl-
oligosaccharide was determined by HPLC.
Results. Fucosyl-disaccharide was synthesized by transgalactosilation in a
concentration of 6 g / ml. This disaccharide consists of galactose and fucose
determined by acid hydrolysis. And it was purified by fermentation with the use of

135
Saccharomyces cerevisea var. chevaliere eliminating monosaccharides (glucose and
galactose from hydrolysis of lactose) where it was observed that fermentation depleted
100% of these monosaccharides.
Conclusions. The synthesis of a fucosyl-oligosaccharide by -galactosidase proved to
be a good alternative for the production of this compound. The fact that the
oligosaccharide synthesized by this method contains fucose in its structure is of great
importance, since the main objective of this study was to synthesize compounds
structurally similar to those present in breast milk.

T-05 CHARACTERIZATION AND DETERMINATION OF PHENOLIC COMPOUNDS,
ANTHOCYANIN CONTENT AND REDUCING SUGARS PRESENT IN MINOR FRUITS
(BLUEBERRY, RASPBERRY, STRAWBERRY AND BLACKBERRY) HARVESTED IN
MEXICO.
Fragoso Castro, Inari Idalith
1
, Lpez Cortz, Ma. del Socorro
1
, Cornejo Mazn, Maribel
1

1
Instituto Politcnico Nacional. Escuela Nacional de ciencias Biolgicas. Departamento de Biofsica.
Prolongacin de Carpio y Plan de Ayala s/n Col. Santo Toms. C.P. 11340. Mxico, D.F. Tel: 57296300.
Ext. 62314.
dampness_123@hotmail.com

The very important nutritional value of fruits and vegetables is well known, since they
are the best carriers of vitamins, minerals, dietary fiber, phenolic antioxidants,
glucosinolates and other bioactive substances, such as anthocyanins and phenolic
compounds, which have pharmacological and therapeutic properties such as reduction
of coronary artery disease, anti-cancer, anti-tumor, anti-inflammatory and antidiabetic
effects. Likewise, the phenolic compounds are a complex group of substances including
flavonols, anthocyanins and catechins, and may be found in the plants in isolated forms
or binded with sugars (glycosides). Both phenolics and anthocyanins are present in
different fruits, especially in minor fruits such as blueberries, raspberries, strawberries
and blackberries.

136
In the present research was studied the physicochemical properties (total soluble solids,
acidity, pH, density, humidity and reducing sugars) of blueberry, strawberry, raspberry
and blackberry from Michoacan and Jalisco State. Also anthocyanins were
determination by differential pH assay, being the blueberry with the highest amount of
anthocyanins with 404.6 0.5 mg eq of cyanidin-3-glucoside (C3G)/kg fw, followed by
blackberry with 104.76 0.31 mg eq C3G/kg fw, strawberry with 5.02 0.06 mg eq
C3G/kg fw and raspberry with 3.50 0.07 mg eq C3G/kg fw. Phenolic compounds were
determined by the Folin-Ciocalteu assay, obtaining 380.470.8 mg EAG/100g fw for
blueberry, followed by blackberry with 378.29 1.8 mg EAG/100g fw, raspberry with
154.052.67 mg AGE/100g fw and Strawberry with 135.87 13.3 mgEAG/100g fw, all
analyzes were performed by triplicate.

T-06 PHYSICOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT PROPERTIES
OF GRAPEFRUIT JUICE (Citrus paradisi).
Hernndez Fernndez M. A
1
. Arellano Crdenas S.
1
. Lpez Cortz M. S.
1

1 Instituto Politcnico Nacional. Escuela Nacional de Ciencias Biolgicas. Depto de Biofsica. Laboratorio
de Investigacin II. Prolongacin de Carpio y Plan de Ayala S/N Colonia Sto Toms. CP.11340. Mxico,
D.F. Tel. 015557296300 Ext 62314. mikkess_10@hotmail.com

Grapefruit (Citrus paradisi) comes from the tree of the Rutaceae family, cultivated for its
fruit. It is a spontaneously produced hybrid between Shaddock (Citrus maxima) and
sweet orange (Citrus sinensis). It has been shown that consumption of citrus favors the
prevention of degenerative and chronic diseases. Citrus benefits have been mainly
attributed to the presence of bioactive compounds, such as phenols (flavanones,
glycosides, hydroxycinnamic acid), Vitamin C and carotenoids. Flavonoid components
of the grapefruit juice are abundant, particularly naringin. Antioxidant activity,
antiinflammatory and antiulcerica are functional properties of naringin. The top
producing states are: Veracruz, Michoacan and Tamaulipas.

137
Physicochemical analyzes (determination of pH, Brix, acidity), vitamin C, phenolics,
antioxidant activity and amount of naringin were made to natural grapefruit juice (Citrus
paradisi)
Total phenol determination was performed by Folin-Denis, obtaining a value of
1370.8333 86.2168 ppm of tannic acid. Antioxidant capacity was quantified with
DPPH, getting 2819.0221 18.0448 mol of trolox. Ascorbic acid determination was
performed with indophenol assay, obtaining 48.0215 0.2003 mg/100 mL. Naringin
content was determined by the Davis method, obtaining 1.6053 0.0211 mg/mL.

T-07 STRATEGIES TO PRODUCE RECOMBINANT GLYCOPROTEINS IN
FILAMENTOUS BACTERIA: FROM SHAKE FLASK TO BIOREACTOR IN THE
PRODUCTION OF APA FROM Mycobacterium tuberculosis IN Streptomyces lividans
Ramss A. Gamboa-Suasnavart
1,2
, Luz D. Marn-Palacio
1,2,5
, Wolf Kloeckner
4
, Jos A. Martnez-Sotelo
3
, Clara Espitia
3
, Luis Servn-Gonzlez
2
, Norma A. Valdez-Cruz
2
, Jochen Bchs
4
and Mauricio A.
Trujillo-Roldn
1,2
*.
1
Unidad de Bioprocesos,
2
Departamento de Biologa Molecular y Biotecnologa,
3
Departamento de Inmunologa. Instituto de Investigaciones Biomdicas, Universidad Nacional Autnoma
de Mxico. AP. 70228, Mxico, D.F., CP. 04510, Mxico.
4
AVT Biochemical Engineering, RWTH
Aachen University, Aachen, Germany.
5
Universidad EAFIT. Medelln -Colombia. maurotru@gmail.com

Alanine and Proline rich Antigen (APA) from Mycobacterium tuberculosis has been
proposed as a new candidate to generate a new vaccine or diagnosis kit against
Tuberculosis. Its immunological activity depends of its O-mannosylation pattern. In this
work, the three most commonly used shake flasks (SF) geometries in filamentous
bacteria cultures were evaluated (Conventional Erlenmeyer, baffled and a conventional
with a spring at the bottom). These geometries provide different aeration/mixing
conditions. The impacts of those variations were evaluated on the S. lividans bacterial
pellet morphology (by image analysis), bacterial growth (dry weight), rAPA production
(Western Blot and SDS-PAGE) and O-mannosilation (MALDI-TOF). Besides bacterial
growth remained unchanged, bacterial morphology is affected by the flask geometry.

138
Moreover, morphology is inversely correlated with rAPA productivity and its O-
mannosylation degree, being higher in smallest aggregates (5 mannoses attached to C-
terminal) and lower in biggest ones (2 mannoses). Additionally, rAPA production was
scaled-up from coiled SF to 1.0 liter bioreactor based on S. lividans morphology.
Previous reported data from the literature on the effect of agitation on Streptomyces sp.
morphology were used, in order to obtain favourable culture parameters to operate the
bioreactor in rAPA production by S. lividans. Scale-up was successfully achieved
obtaining same morphology and similar O-mannosylation degree, finding up to five
mannoses SF and up to six in bioreactor. Data suggests that manipulating culture
conditions, that affects filamentous bacterial morphology, is probable to favour the
production of a specific O-mannosilation pattern with the possibility to manipulate the
immunogenic properties.

T-08 INDUSTRIAL GLYCOBIOLOGY OF Burkholderia tropica AS SELECTED STRAIN
FOR CARBOHYDRATE MODIFICATION.
Galindo-Msico J.H.
(1)
Contreras-Esquivel J.C.
(1)
*, Estrada de los Santos P.
(2)

1) Universidad Autnoma de Coahuila, Departamento de Investigacin en Alimentos, Facultad de
Ciencias Qumicas, Blvd. Venustiano Carranza e Ing. Jos Crdenas s/n., Colonia Repblica. Saltillo
25280, Coahuila, Mxico. Tel: (844) 415-9534 y 416-9213, e-mail: logan_joma@hotmail.com
2) Instituto Politcnico Nacional. Departamento de Microbiologa, Laboratorio de Microbiologa
Industrial. Escuela Nacional de Ciencias Biolgicas, IPN Telfono: +52 (55) 57296000 Extensin: 62382,
E-mail: pestradadelossantos@gmail.com

ABSTRACT. Carbohydrate-oxidases are enzymes with widely application in
glycobiology for transformation of molecules for industrial or medical purposes.
Burkholderia tropica is a Gram negative bacterium isolated from industrial crops (sugar
cane) and show potential for oxidation of carbohydrates. The aim of this work was
evaluate the oxidative capacity of Burkholderia tropica fermented extracts on
monosaccharaides and oligosaccharides. Strains of Burkholderia tropica: MMi-786,
TTe-225, MOc-725, BM-273 and MTI-582, B. Ferrarie PAS44T) were procured from

139
Instituto Politecnico Nacional, Mexico DF, Mexico. Fermentation was performed in liquid
medium containing dextrin and water soluble chitosan as carbon source and casein
peptone for nitrogen source for 72 h at 37C and 250 rpm. The fermented extract and
catalase were used for enzymatic oxidation of monosaccharides (inositol, glucose,
glucosamine, xylose, arabinose, galactose, and rhamnose) and oligosaccharides
(maltose, cellobiose, lactose and raffinose). Oxidation analysis was incubated at 38 C
for 96 hours. The results showed that Burkholderia tropica BM-273 strain showed
highest oxidation capacity for maltose, cellobiose, lactose, raffinose, glucose,
glucosamine, xylose, galactose and rhamnose. The enzymatic activity of this strain was
negative for inositol and lactose hydrolysis.

1*) Corresponding autor. Tel.: +52 844 416 9213: fax: +52 844 439 051, e-mail address:
coyotefoods@hotmail.com

T-09 NP-HPLC GLYCOSYLATION ANALYSIS OF RECOMBINANT PROTEINS
PRODUCED BY ANIMAL CELL CULTURE.
Vanessa Hernndez
a
, Jos A. Serrato
b
, Octavio T. Ramrez
a
and Laura A. Palomares
a

a
Instituto de Biotecnologa, Universidad Nacional Autnoma de Mxico. Av. Universidad 2001 Col.
Chamilpa, Cuernavaca Morelos. C.P. 62210, Mxico. Tel. (777) 3291617,
b
Enfermedades Respiratorias Ismael Coso Villegas. Calz. Tlalpan 4502 Col. Seccin XVl Deleg. Tlalpan
D.F. C.P. 14080
vanessa@ibt.unam.mx

A large number of proteins produced by mammalian cells are glycoproteins. These
proteins have highly diverse oligosaccharide structures (glycans) covalently attached to
the polypeptide chain. As glycans are located on the surface of proteins, their
occurrence plays an important role in the molecule biological activity. Due to the
structural complexity and ubiquitous influence of glycans on proteins, high performance
analytical techniques are required for a proper structural and functional understanding
of the proteins and its glycans, such as Normal Phase Liquid Chromatography (NP-

140
HPLC). The N-glycosylation profile of Secreted Alkaline Phosphatase (SeAP), BCF2
Monoclonal Antibody (MAb) and Influenza virus Hemagglutinin (HA) produced by cell
culture was performed by NP-HPLC. Differences were observed among proteins as well
as between the same protein produced under different culture conditions or by a
different host. SeAP produced during perfused CHO cell cultures had a higher content
of sialylated structures than fed batch cultures (16.6 +/- 3.4% and 13.4 +/- 2.4%
respectively). However, SeAP produced by insect cells had mostly paucimannose and
high mannose glycans. Different subtypes of HA produced by insect cells were
analyzed. Each subtype showed a characteristic N-glycosylation profile. A MAb
produced by hybridoma cells in batch cultures showed a high microheterogeneity of
glycosylation. Agalactosylated, mono and di-galactosylated glycans accounted for the
majority of structures. Little amounts of sialylated glycans were also identified (<10%).
The results obtained show the power of the analysis tool presented here.

T-10 OBTAINMENT A NUTRACEUTICAL OF MARINE ORIGIN BY MICROWAVE-
ASSISTED GREEN TECHNOLOGY.
Ramn-Delgado M.J.
1,2
, Contreras-Esquivel J.C.
1,2
., Couto S.A.
3
., Casabuono A.
3
., Morlett-Chvez
J.A.
1
, Carranza-Rosales, P.
4

1
Departamento de Investigacin en Alimentos, Facultad de Ciencias
Qumicas, Universidad Autnoma de Coahuila, Saltillo 25280, Coahuila, Mxico. Tel: (844) 4161238.
2
Coyotefoods Biopolymer and Biotechnology S.R.L.MI, Saltillo 25000, Coahuila, Mxico.
3
Departamento
de Qumica Orgnica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires,
Argentina.
4
CIBIN-IMSS, Monterrey, Nuevo Leon, Mxico. E-mail: jes_ary8@hotmail.com

Fucoidan is a sulphated polysaccharide with various biological activities such as
anticoagulant, anticancer, antitumor, antioxidant, among others. The aim of this work
was depolymerize fucoidan by free-solvent microwave-assisted treatment and evaluate
its biological activities. Fucoidan (4%, w/v) was depolymerizated at 120C for 0, 0.5, 1,
3, 6, 12, 24 and 48 minutes. Anticoagulant activity (aPTT) of fucoidan hydrolysates was
evaluated in the range 0 to 400 g/mL. For aPTT assay fucoidan (400 g/mL)

141
depolymerized for 48 minutes showed a clotting time above 2327.4 seconds, whilst non-
depolymerized sample was about 3681.3 seconds (Figure 1a). Control samples without
fucoidan showed a clotting time of 25 seconds. Infrared spectroscopy revealed the
presence of sulfate groups in treated as non-treated fucoidan (0 and 48 minutes
depolymerized) in the region of 1229 cm
-1
(Figure 1b) These results indicate not lose of
sulfate groups during depolymerization and maintain anticoagulant activity. Low
molecular weight fucoidan is a novel additive for development of functional foods
produced by microwave heating under green technology.

Fucoidan (g/mL)
a
P
T
T

(
s
e
c
)
0
2500
3000
3500
4000
0 min
48 min
0 5 10 20 50 100 400
22.6
34.9
47.7 46.9 46.2
60.3 59.2
121.5
125.1
372.6
274.8
3681.3
2327.4
22.6

A
B
Effect of level of fucoidan and microwave treatment on anticoagulant (A) and infrared spectrum of native
fucoidan and treated fucoidan by microwave heating for 48 min (B).

T-11 IDENTIFICATION OF MOLECULAR COMPLEX (CCL- -GLUCOSIDASE) FROM
NATIVES MAIZE OF OAXACA STATE
Snchez-Esperanza F
a
*., Aragn-Cuevas. F
b
., Martnez-Cruz M
a
.
a
Unidad de Bioqumica e Inmunologa del Instituto Tecnolgico de Oaxaca
Ave. Ing. Vctor Bravo Ahuja # 125 esq. Clz. Tecnolgico C.P. 68030 Oaxaca, Oax.
b
Instituto Nacional de Investigaciones Forestales, Agrcolas y Pecuarias
Campo Experimental Valles Centrales
Correo electrnico: faby.sanchez.e.@hotmail.com

B
wave number (cm
-1
)
700 1000 1300 1600 3000 3500
A
b
s
o
r
b
a
n
c
e

(
n
m
)
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
Control
48 min
carbohydrate region
1638 cm
-1
1733 cm
-1
1229 cm
-1
840 cm
-1

142
This work data describes the analysis of ten local landraces of maize of the state of
Oaxaca in Mexico and a hybrid (Berentsen SB- -glucosidase and lectin
activity it was monitored in raw extracts of coleoptiles of five days of growth. The data
showed differences in two of the analyzed Tuxpeo and Conejo races, compared with
wild-type K55 used as control. The Conejo showed 9,758 UHA/mL of lectin and 164
- glucosidase and the Tuxpeo 9,136 UHA/mL and 178 U/mg, both
cases the activities were greater to the control (SB-302).The maize extracts analysis
from Tuxpeo and Conejo races, from gel filtration data, showed low contained in larges
-glucosidase-lectin with high molecular mass
complex > 1 X10
6
D, it is showed a containing of the order to 80%, of a component of
-glucosidase dimer. Evidences are presented that both
analyzed maize race, they are similar to wild-type K55 previously reported Esen Et al
(2000).

T-12 EFFECT OF MILD HYPOTHERMIA ON SECRETION AND QUALITY OF
RECOMBINANT GLYCOPROTEIN tPA IN CHO CELLS.
Bedoya-Lpez Andrea
1
, Estrada Karel
2
,Sanchez-Flores Alejandro
2
, Ramrez Octavio T
3
, Trujillo-Roldn
Mauricio A.
1
, Valdez-Cruz Norma A.
1
.
1
Departamento de Biologa molecular y Biotecnologa, Instituto de
Investigaciones Biomdicas, UNAM.
2
Unidad Universitaria de Apoyo Bioinformtico, Instituto de
Biotecnologa, UNAM.
3
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologa,
UNAM. Av. Universidad No. 3000, Col. Universidad Nacional Autnoma de Mxico, CU. Delegacin
Coyoacn, CP 05410. Tel 56229192. adrivaldez1@gmail.com

Recombinant glycoproteins (RGP) are widely used in the treatment of many diseases.
However, their manufacturing and processing are expensive hence the need to improve
their production. Temperature downshift (TDS) from 37C to 28-34C normally increase
culture length and specific productivity (Q
p
) of RGP. Particularly, the Q
p
of tissue
plasminogen activator (tPA) increased 1.6 fold in Chinese hamster ovary (CHO) cells
line TF70R with TDS. The objective of this work is to understand, at molecular level, the
effect of TDS over TF70R by transcriptomic and secretomic analysis to identify some

143
elements that could improve Q
p
. Biphasic cultures were growth at 37C for 48 h, then
changed to 30C and a comparison with the control was made (37C). Transcriptomic
analysis were done at 24 and 48 h after TDS, and compared with the sample obtained
just before TDS. Transcriptomic analysis showed differentially expressed genes related
with secretion (endoplasmic reticulum, Golgi, vesicles, and cytoskeleton). Around 31
genes were overexpressed and 44 repressed in 24 h after TDS and 28 genes up-
regulated and 21 down-regulated at 48 h after TDS. Samples for SDS-PAGE 2D
analysis were collected 48 h after TDS. Some differences were observed in those
secreted at 30C (6 spots) with respect to control at 37C (5 spots). Using western blot,
differences in molecular weight and in isoelectric point of the RGP were detected,
suggesting changes in the glycosylation profile.

T-13 DEVELOPMENT OF SINGLE CHAIN FV ANTIBODY FRAGMENT AGAINST -
GAL EPITOPE WITH POTENCIAL USE FOR IN SITU DIAGNOSIS OF HUMAN
PAPILLOMAVIRUS. Manzo Sandoval A. 1, Gavilondo Cowley J.V. 2, Medina Escutia
M.E. 3, Medina Flores Y. 3, Ramn Gallegos E. 1. Escuela Nacional de Ciencias
Biolgicas (IPN), Av. Wilfrido Massieu Esq. Cda. Miguel Stampa s/n C.P. 07738,
Mxico D.F. Tel. 57 29 60 00 ext. 52399 (1). Centro de Ingeniera Gentica y
Biotecnologa (CIGB), Ave 31 e/ 158 y 190, Playa, P.O. Box 6162, La Habana 10600,
Cuba. Tel. 53-7-2718008 (2). Instituto de Diagnstico y Referencia Epidemiolgicos
(InDRE), Prolongacin de Carpio 470 Col. Casco de Santo Tomas C.P. 11340, Mxico
D.F. Tel. 53 42 75 50 ext. 260 (3). eramong@ipn.mx

The -Gal epitope is an oligosaccharide component of glycolipids and glycoproteins
displayed on the cell surface of non-primate mammals but not in humans. However, -
Gal is present in cervical mucus, koilocytes and keratinocytes from pacients with
cervical cancer and human paillomavirus (HPV). Our working group generated an anti
-Gal specific IgM monoclonal antibody (mAb) which recognizes cells infected with
HPV-16 (cervical cancer biopsy sections) and HPV-18 (HeLa). The objective of this
work is to obtain a single chain Fv (scFv) from anti -Gal mAb, to be used in
colposcopic diagnosis. Primers were designed to amplify the light (VL) and heavy (VH)
mAb variable regions. Total RNA was extracted from hybridoma ATCC PTA-13359 that
produces anti -Gal specific mAb, cDNA was synthesized and PCR was standardized
for VH and VL amplification. The PCR products were sequenced and were design new
primers to be used in an overlapping PCR technique, for the insertion of a short linker or
long linker between VH and VL. It was determined that VL is constituted by 318 pb and

144
VH by 363 pb. It were obtained two products of 700 pb by overlapping. Monobodies and
diabodies generated will be analyzed in the BIACORE system and will be tested, in
positive HPV cell lines 16 and 18 and in cervical cancer biopsy sections. It were
constructed two types of scFvs and currently we are working on the expression of these
fragments that can be used in vivo diagnosis of HPV.

T-14 STARCH AS DELIVERY SYSTEM FOR ORAL VACCINATION
Guilln D., Moreno-Mendieta S., Espita, C., Rodrguez-Sanoja, R.

Instituto de Investigaciones Biomdicas, Nacional Autnoma de Mxico; Av.
Universidad 3000 C.U. Distrito Federal, 04510. Tel 56229191
romina@biomedicas.unam.mx

New vehicles for mucosal drug delivery are currently under study due to the advantages
that this route presents over parenteral or subcutaneal administration. Microparticles are
among the most studied vehicles for oral administration since their size improves the
capture and/or presentation of peptides or proteins in the intestinal environment.
Starch is an abundant substrate, biocompatible, inexpensive, with an authorized use in
food and pharmaceutical industry, as well as being naturally particulate, characteristics
that make it ideal for use as oral vehicle for therapeutic protein and antigens.
In this study we propose a new immobilization and delivery system using raw starch
microparticles and a starch binding domain (SBD
tag
) fusion protein. The heat shock
protein alpha crystalline from Mycobacterium tuberculosis and the fragment C of tetanus
toxin (Tc) were used as model antigens. These immobilized proteins were orally
administered with the purpose of analyze and evaluate the systemic response in mice.
We demonstrated starch immobilization through the SBD
tag
allows antigen delivery in
mucosal associated lymphoid tissue, as determined by the serum levels of IgG specific
response detected in mice. Since no extra adjuvants were used we remark the system
may be suitable for delivery and presentation of antigens, haptens or proteins.

T-15 THE CARBOHYDRTE BINDING CAPACITY AS A TOOL FOR THE
PURIFICATION OF RECOMBINANT PROTEINS. Rodrguez-Sanoja, R.
1
, Guilln D.
1
,
Aguilera, P.
1
, Moreno-Mendieta S.
1
, Snchez S.
1
, Farres A.
2
1
Instituto de Investigaciones Biomdicas,
2
Facultad de Qumica. Universidad Nacional
Autnoma de Mxico; Av. Universidad 3000 C.U. Distrito Federal, 04510. Tel 56229191
romina@biomedicas.unam.mx

Affinity tags are the most used tools for recombinant protein purification. These
tags are either proteins or short that confer binding specificity to specific ligands
immobilized on a solid support. Affinity resins are generally expensive and consequently
not useful for large-scale protein purification. We have designed a starch binding
domain (SBD
tag
) as a tag for purification or immobilization of proteins on raw starch,

145
starch derivatives or starch analogues. The natural availability of starch and its chemical
properties, e.g., that it is naturally particulate, inert, and approved for pharmaceutical
and human uses, makes it an attractive, low-cost matrix for biotechnological processes.
To demonstrate the efficiency of the system, four proteins of different origins
were fused to a His
tag
, the most common tag used for protein purification, and the
proposed SBD
tag.
Resulting chimeras were purified by affinity chromatography using a
metal ion charged column (His
tag
) or a coupled -cyclodextrin sepharose column
(SBD
tag
). The results showed that the SBD
tag
is superior to the His
tag
for protein
purification. The efficient adsorption of the fusion proteins to raw corn starch was also
demonstrated, and two fusions were selected to test purification directly using raw
starch from rice, corn, potato, and barley. The two fusion proteins were successfully
recovered from crude bacterial extract using raw starch, thus demonstrating that the
SBD
tag
can be used as an efficient affinity tag for recombinant protein purification on an
inexpensive matrix, allowing the use of the system both laboratory and industrial scale.

I-01 CITOKINES AND GALECTIN-3 IN ACUTE GLOBAL CEREBRAL ISCHEMIA IN
RATS TREATED WITH MELATONIN
Garduo Ros D
1
, Torner Aguilar L
2
, Cervantes Alfaro M
3
, Letechipa Vallejo G
3
and Fenton Navarro B
1*
.
1) Laboratorio de Glicobiologa. Divisin de Estudios de Posgrado, Facultad de Ciencias Mdicas y
Biolgicas Dr. Ignacio Chvez, UMSNH. Dr. Rafael Carrillo esq. Dr. Salvador Gonzlez Herrern
S/N. Col. Bosque-Cuauhtmoc, Centro CP 58000. Morelia, Mich. Mxico. Tel: (01-443)- 312-0014 y
312-0510 ext. 234. bertha00_mx@yahoo.com
2) Centro de investigacin Biomdica de Michoacn, IMSS
3) Laboratorio de Neurociencias, Posgrado, Fac. Cs. Med y Biol. UMSNH

In the Acute Global Cerebral Ischemia (AGCI) the inflammatory response plays a crucial
role in brain damage. Cytokines and Galectin-3 (Gal-3) may be related to brain damage
after ischemia. In AGCI there are no reports circulating levels variations in a time frame
around this event. Methods: Circulating concentrations 0, 30 min, 6 h and 24 h
subsequent to I/R of TNF-, IL-6, IL-10, and Gal-3 were quantified using commercial
ELISA kits in adult male rats. The four-vessel occlusion method was used, followed by 6
h iv infusion of melatonin or vehicle. Also the locomotion activity (open field test) was
INMUNOGLICOBIOLOGA

146
evaluated after 24h. Two groups of control animals Sham-melatonin and sham-vehicle
were also included in the study. Results: AGCI followed by reperfusion induced a
decrease in serum concentrations of TNF-, and an increase in IL-6 at 24 h after
ischemia, whereas than melatonin caused a significant reduction in serum
concentrations of the cytokines at 6 h (TNF-) and 24 h (IL-6) after I/R. Circulating
levels of Gal-3 was reduced from 30 min after I/R which could be related to the increase
of reactive oxygen species. Neuroprotective treatment with melatonin and ischemia
caused a reduction of serum concentrations of Gal-3 at 6 h. The results of the open field
test showed less damage in the hippocampus and less anxious state in ischemic
animals treated with melatonin compared to ischemic animals treated with vehicle.
Conclusion This is the first report of the involvement of cytokines and Galectin-3 in
immediate times following AGCI.

Keywords: Galectin-3, Cytokines, Global Cerebral Ischemia, Inflammation, Melatonin, Rats.

I-02 PEPTIDE MIMOTOPES SELECTED BY PHAGE DISPLAY FROM A REGION OF E. coli O157 LPS INDUCE
ANTIBODY EXPRESSION AGAINST O157 LPS
Navarro A
1
, Hernndez U
1
, Len L A
1
, Licona D
1
, Eslava C
1
.
1
Departamento de Salud Pblica, Facultad de Medicina. Universidad Nacional Autnoma de Mxico. Ciudad
Universitaria, Coyoacn, C.P. 04510 Mxico. D. F. Tel 56232257. arnava@unam.mx
Introduction. The lipopolysaccharide (LPS) is an important component of the Gram
negative bacteria cell wall. The LPS is composed of lipid A or endotoxin, a core region
and repeating units consisting of carbohydrates. These structures are involved in both
the pathogenesis of the microorganism and the host immune response. Objective.
Using Phage display to selected peptides, evaluate the immunogenic properties of
selected peptide mimotopes of E. coli O157 LPS. Material and methods. Biopanning
was performed with a 12 amino acid peptide library (Biosynthesis) and rabbit IgG

147
polyclonal anti-O157 LPS was used to select phagotopes and obtain the peptide
sequence. With the peptide sequence, synthetic peptides conjugated to KLH were
developed and used to immunize rabbits. The reactivity of the sera was evaluated using
ELISA against both the homologous peptides and O157 LPS. The peptide PS12 was
incubated at 37C with anti-LPS O157 serum to produce a competitive inhibition assay
for the binding of anti-LPS O157 antibodies to the homologous antigen. Results. The
peptides obtained presented the motif SLXP- and PPLWX- like consensus sequence.
Four rabbit sera against the peptide-KLH conjugate were obtained, namely SPS5/KLH,
SPS11/KLH, SPS/12 and SPS16/KLH. The SPS12/KLH serum showed a positive
reactivity against unconjugated peptide PS12 at a 1:800 serum dilution and a peptide
concentration of
The
results of the competitive inhibition assay looking at the reactivity of O157 anti-serum
with the homologous antigen showed inhibition by the PS12 peptide at concentrations
from . Sequencing of PS12 peptide shows it to be a mimotope of
O157 LPS. Further tests demonstrate it to have immunogenic properties that induce the
production of antibodies, which react with both the homologous peptide and O157 LPS.
In addition, the PS12 peptide was shown to share the same binding site of anti-serum
O157 to LPS O157, suggesting that this peptide could be potentially useful in designing
a protective immunogen.

This work was supported by grants from DGAPA/PAPIIT IN218311 (UNAM).
I-03 IDENTIFICATION OF HEMOCYTES LECTIN HOMORECEPTOR OF THE
CRAYFISH Cherax quadricarinatus.
Snchez Salgado J. L.
1
, Pereyra Morales A.
1
, Alpuche Osorno J. J.
2
, Vivanco Rojas O.
1
, Agundis Mata
C.
1
, Zenteno E
1
.
1
Laboratorio de Inmunologa, Departamento de Bioqumica, Facultad de Medicina,
Universidad Nacional Autnoma de Mxico, Mxico.
2
Instituto Tecnolgico de Estuidos Superiores de
Champotn. *jlsanchezsalgado@gmail.com

148
Introduction. Crayfish Cherax quadricarinatus presents a serum lectin (CqL) that
recognizes Gal, Glc and NeuAc. The function of this lectin still remains uncertain in the
organism. Other lectins have the ability to recognize carbohydrates in the pathogen cell
surface and participate in the defense mechanisms of crustaceans. In this study we
identified the hemocyte receptor and the participation of this molecule in the
phagocytosis mechanism.
Methodology. We obtained the hemolymph and cells samples from male adult
organisms. CqL was purified by affinity chromatography and coupled to Biotin for the
incoming experiments. We identified the homo receptor of CqL in the cell membrane of
hemocytes by immunocytochemistry and Western blot technique. Moreover, the effect
of this lectin was determined in the production of free radicals in the hemocytes by NBT
reduction technique.
Results. In WB of hemocytes, the CqL recognizes a 120 kDa fraction composts CqL
homoreceptor in the membrane cells. This protein is located in the cell membrane of the
hyaline hemocytes and different molecules trigger its expression of the receptor. The
production of free radicals is significant higher (p < 0.025) in presence of CqL. The
effect was inhibited by 200 mM of Galactose and Glucose as well as by superoxide
dismutase (SOD).
Conclusions. These results suggest that Cherax quadricarinatus presents lectin
hemocytes homoreceptor specific for CqL and participates in the production of free
radicals, this suggest that this lectin intervenes in intracellular signal pathways from
recognition of Pathogen associated molecular patrons.

Financed by PAPIIT IN226211 (UNAM, Mxico).

I-04 A PARTIAL CHARACTERIZATION OF A SERIC LECTIN OF THE SHRIMP
Litopenaeus vanameii.
Vivanco-Rojas Oscar 1 , Martin-Manzo Miriam 2 , Campa Angel 2 , Pereyra Ali 1 , Snchez-Salgado Jos
Luis 1 , Zenteno Edgar 1, Agundis Concepcin 1 . Laboratorio de inmunologa, Departamento de
Bioqumica, Facultad de Medicina, UNAM, P.O. Box 70159, 04510, D. F. Mxico. Tel: +52 (55) 5623-

149
2168; Fax: +52 (55) 5616-2419, 1. Centro de Investigaciones Biolgicas del Noroeste, S.C. (CIBNOR),
La Paz, Baja California Sur, Mxico 2. c_so99@hotmail.com.

Invertebrates lack an adaptive immune system, but they have developed various
defense mechanisms by which they recognize surface determinants on potential
pathogens. These mechanisms include hemolymph coagulation, cell agglutination,
melanization, and phagocytic activity, production of reactive oxygen intermediates, and
release of antimicrobial peptides. Because of their specific recognition of sugar
determinants in the wall or the capsule of bacteria, lectins have been suggested to
participate in innate immune response by inducing bacterial agglutination, or as
opsonins, by enhancing phagocytosis rates of microorganisms by hemocytes. A lectin
from the hemolymph of adult freshwater prawn was purified in a single step by affinity
chromatography on glutaraldehyde-fixed stroma from rat erythrocytes. The lectin is a
tetramer whit MW of 83.1, 64, 46.2 and 33.3 kDa; each protein is composed by glycine
and asparagine; and methionine, tyrosine, histidine or proline, with relatively lower
contents. The lectin agglutinate rat and rabbit, when erythrocytes are treated whit
syalidase or trypsin, the activity was higher than erythrocytes without treatment. The
hemagglutinating activity of the lectin was inhibited by fetuin and lactose. The
characteristics founded to this lectin suggest recognition to glycolipids as no other lectin
identified in crustaceans.PAPIIT IN226211 (UNAM, Mxico).

I-05 INCREASED SIALYLATION ON MACROPHAGES BY LPS TREATMENT.
Lpez Castillo Misael, Gonzlez Christen Judith. Lab. Inmunidad Innata. Facultad de Farmacia UAEM.
Av. Universidad 1001. Col Chamilpa. Cuernavaca Mor. Cp 62209. Tel 777-3297089.
misael.pez@me.com, judith.gonzalez@uaem.mx

BACKGROUND: The macrophage (Mf) and dendritic cells (DC) are important immune
cells that play key roles in activating immune responses during infection by linking the
hosts innate and adaptive immune responses against pathogen infections. TLR are a

150
family of receptors that recognize pathogens and activate these cells. It has been
suggested that TLR4 activation by LPS increase the sialidase Neu3 activity on the
murine DC, and in Mf and DC TLR 2,3 or 4 ligand binding induces Neu-1 sialidase
activity. Surprisingly the overall change in the sialylation profile of these activated cells
has not been studied METHODS. THP-1 cells (ATCC Tib 202) were firstly differentiated
to macrophages with 10 nM PMA for 72 h. Following, the macrophages were treated
with PBS or 20 g/mL of LPS (E. coli, Sigma) for 24-48 hrs, washed and stained with
SNA-biotin or MAL-I-biotin and streptavidin-HRP. Positive cells were evaluated by light
microscopy. RESULTS: Macrophages without treatment showed the lowest reactivity for
both lectins MAL-I (32%) and SNA (38.5%). After LPS treatment, MAL-I binding
increased to 67.5 (24 hrs) and 74.5% (48 hrs). Meanwhile Sial 2,6 terminal glycan
(SNA binding) also increased to 70.5%, showing not change between the two times. In
order to associate these results with the immune response of macrophage, we are
evaluating secretion profile of TNF, IL-1 , IL-6, IL-10, TGF- and sialidases activity.
CONCLUSION. Activation of macrophages with LPS induces an increase in terminal
2,6-linked Sias and Sia 2-3-Gal residues.

I-06 ACTIVATION OF NAVE CD4+ T CELLS IS ASSOCIATED TO
HYPERSIALYLATION AT THE EXPENSE OF GANGLIOSIDE SYNTHESIS
Villanueva Cabello Tania Mara
1
, Martnez-Duncker Ivn
1
.
Laboratorio de Glicobiologa Humana, Facultad de Ciencias, Universidad Autnoma del Estado de
Morelos
1
. Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos. C. P. 62209. duncker@uaem.mx

Introduction: Human CD4+ T lymphocytes organize the adaptive immune response after
being activated by antigen presenting cells. Activation of these cells is characterized by
specific cytokine secretion but also by glycosylation changes that play an important role

151
in their homeostasis. Activation has been reported to induce an asialophenotype
because of reduced binding of SNA (2,6) and MAL II (2,3) lectins caused by
downregulation of ST3Gal I and ST6Gal I. Nonetheless in this work we show that
activation of CD4+ T human cells is in fact associated to increased de novo sialylation
associated to 2,8 sialic acid glycoconjugates. Methods: Human CD4+ T cells were
activated for 24, 48 and 72 hours. At each time the incorporation of Sia de novo was
determined on surface glycoconjugates together with gangliosides and sialyltransferase
expression. Results: Activation was associated to an increase in the expression of "b"
series gangliosides and the sialyltransferase ST8Sia 1 gene responsible for their
synthesis. Conclusions: Overexpression of ST8Sia 1 participated in the increased de
novo Sia incorporation at the expense of b" series ganglioside synthesis that could play
an important role during activation.

I-07 FERRITIN GLYCATED AND GLICOXIDATED INCREASED EXPRESSION AND
ACTIVATION OF TOLL-LIKE RECEPTORS 2 AND 4 IN HUMAN CD14 CELLS. Galvn
Moroyoqui Jos 1, Candia Plata Ma. del Carmen 2, Martnez Soto Juan 3, Soto Guzmn Adriana 1, ,
Garca Villa Denisse 1, Carvajal Dosamantes Armando 2, Soto Gastelum Cecilia 2, Lpez Soto Luis 1.
Depto. de Medicina y Ciencias de la Salud 1, Laboratorio estatal de Salud Pblica 2, Depto. de Ciencias
Qumico Biolgicas 3. Hermosillo Sonora. Blvd. Luis Encinas y Rosales S/N Col. Centro, CP 83000. Tel.
(52) 6622592269 ext 27.
galvan.moroyoqui.jm@guayacan.uson.mx

Introduction: Recent studies it has been reported a possible association between
increased serum ferritin levels with type 2 diabetes mellitus (T2DM) and the degree of
atherosclerosis in these patients. Possible biochemical mechanism by which
hyperferritinemia contributes to T2DM development is currently unknown. During the
initiation and progression of atherosclerosis in T2DM non-enzymatic glycation of
proteins generates advanced glycation and glycoxidation endproducts (AGEs), which
contribute to the development and progression of atherosclerosis. It is likely that the

152
degree of hyperglycemia and hyperferritinemia in T2DM could favors that ferritin
undergoes modification by glycation and glycoxidation as has been observed with other
serum proteins, contributing to the activation of CD14 cells involved in inflammatory
responses during atherosclerosis development. Methods: We prepared in vitro glycated
and glicoxidated ferritin, which were used to stimulate CD14 cells. Was also evaluated
the role of MyD88 and NF-kB signaling pathways using specific inhibitors of these
signaling pathways. Results: We observed that glycated and glicoxidated ferritin
increased expression of Toll-like receptors 2 and 4 of CD14 cells, and increased
cytokines IL-6 and IL-8 production. Additionally noted that these changes dependent on
MyD88 and NF-kB signalong pathways. Conclusions: These results allow us to suggest
that the modification by glycation and glycoxidation of ferritin may contribute to the risk
of vascular complications in diabetic patients by inducing an inflammatory type
response in CD14 cells, activating signaling pathways dependent on MyD88 and NF -
kB.

I-08 EXPRESSION OF GALECTIN-3 AND ANTIGEN T IN PLATELETS.
Acevedo Prez Miguel ngel
1
1
, Prez Campos E
1
, Pina Canseco MS
1
y Hernndez
Cruz P*
1
.
1
Laboratorio de Glicobiologia CICIMEBIO, Facultad de Medicina y Ciruga, Universidad Autnoma Benito
Jurez de Oaxaca.
2

INTRODUCTION: The platelets (anucleate cells) play a significant role in the
modulation of the innate and adaptive responses, for example, at the start of the
inflammation, angiogenesis, atherosclerosis, lymphatic development and tumor growth.
That way the platelets carried out these different tasks and functions related to the
immune system is an enigma, but the different lines of research suggest that the various
facets of the biology of platelets are important; this includes the unique origin and
structure, the presence of oligosaccharides structures in special antigen T(Gal

153
1,3GalNAc1-O-Ser/Thr) as well as galectin-3. OBJECTIVE: observe the expression of
galectin-3 and antigen T in platelets from healthy individuals. METHODOLOGY: Getting
washed platelets.10 ml of peripheral blood be dumped 5ml in 2 tubes, containing 500 l
of citrate containing dextrose anticoagulant, pH 6.5, were centrifuged at 317 rpm for 10
min at room temperature, making the platelet-rich plasma, which is added an equal
volume of Buffer CGS-EDTA pH 6.5. Centrifuge at 317 rpm for 15 min at room
temperature, the pellet is again suspended in 3 ml of buffer pH 6.5 CGS and centrifuged
at f 317 rpm for 12 min at room temperature. The supernatant is discarded and the
platelets are once more resuspended in 300 l of CGS buffer pH 6.5. 200 Thousand
Platelets, in 300 l of CGS buffer pH 6.5, were incubated with lectin of Artocarpus
integrifolia or Arachis hypogea at concentration of 20 g/ml, for 20 minutes, then 25 l
were placed on a microscope slide. Double labeling of slides were performed as follows:
Plates were labeled with lectins (1g/ml) overnight at 4C and monoclonal anti galectin-
3 only that lectin binding was indirectly recognized with streptavidin-FITC conjugated
and visualized using green filter. Anti-Galectin antibodies were revealed with
streptavidin-Red-X conjugate. Slides were observed with AXIOSCOP 40 microscope
equipped with digital camera AXIOCAM MRC and micrographs analysed with ZEN pro
2011 Software. Some experiments were carried out for flow cytometry using a flow
cytometer (Macs QuantMR MiltenyiBiotec) and data were analyzed with the software of
cytometry MACSQuantify version 2.4 RESULTS: Lectins from Artocarpus integrifolia
and Arachis hypogaea, recognized expression of the antigen T, also expression of
galectin-3 was observed CONCLUSIONS: There is an expression of T antigen and
Galectin-3 in platelets obtained from clinically healthy individuals.

I-09 PARTICIPATION OF CARBOHYDRATES IN MYCOBACTERIAL
PHAGOCYTOSIS OF APOPTOTIC BODIES.

154
Garca Aguilar T.C., Espinosa Cueto P., Mancilla Jimnez R. Depto. Inmunologa. IIB.UNAM. Sede del
Circuito Escolar. Ed. A,1er piso, Ofic.127. Mxico D.F. Delg. Coyoacn. CP. 04510. Tel. 5622-3866.
eretbla@gmail.com

INTRODUCTION
Since the removal of cells dead in apoptosis is an important mechanism aimed to avoid
inflammation, in this study we analyzed in vitro the mechanisms involved in the
phagocytosis of apoptotic cells induced by the O-glicosilated M. tuberculosis 19 kDa
glycoprotein (LpqH). Because there is the translocation of cytosolic mannose containing
glycoproteins to the cell surface of apoptotic cells we considered possible the
participation the mannose receptor (MR) in the removal of apoptotic cells.
METODOLOGY
Apoptotic bodies were obtained by centrifuging the cells treated with cell-walls of M.
smegmatis transformed by LpqH an apoptogenic M. tuberculosis glycoprotein.
Subsequently was purified with magnetic beads coated with Annexin V. Inhibition
assays were performed by pre-incubating the macrophages with N-acetylglucosamine,
mannan and a monoclonal antibody against MR blocker.
RESULTS
In the inhibition assays with 5g of the AcMn with anti-MR was 49% and 10 ug was
76%. Moreover, inhibition of phagocytosis of apoptotic bodies with N-acetylglucosamine
was 52% and mannan was 43%.
CONCLUSIONS
Observations suggest that MR participates in phagocytosis of apoptotic bodies by
binding to carbohydrate residues that could be mannose, fucose and/or N-
acetylglucosamine exposed on the surface of apoptotic bodies. These residues may be
present in lectins that have been exposed or exchanged to the cell surface during
apoptosis.

I-10 MUC2 AND TNF-ARE INCREASED IN TEARS OF PATIENTS WITH

155
ADENOVIRUS OCULAR INFECTION. Santacruz
C 1, Jaimes M 1, Meja H 1, Garfias Y 2, Jimnez-Martnez MC 2. Research Unit, Institute of
Ophthalmology Conde de Valenciana 1. Immunology Lab, Department of Biochemistry, Faculty of
Medicine, UNAM 2. Mxico D.F. Chimalpococa 14 C.P. 06800 tel. 54421700 ext. 3212.
mcjimenezm@institutodeoftalmologia.org

Introduction. Mucin content at the ocular surface of MUC1, MUC3, MUC5AC MUC5B,

MUC7, MUC16 and low levels of MUC2 are consistently detected

in normal human tear
samples. However in pathologic conditions

it has been described modifications in their
quantities as described

in atopic keratoconjunctivitis patients where the increase of

MUC
1, 2 and 4 elicits a significant epithelial disease compared

with insignificant epithelial
disease in eyes of control subjects.

To date, there are no studies describing an
association of ocular

adenovirus infection and modification in tear mucins.
Purpose:To quantify MUC2 and MUC5AC and proinflammatory cytokines in tears
samples obtained from patients

with ocular adenovirus infection.
Methods:Tear samples of twenty four eyes from 12 patients with acute adenoviral

infection determined by PCR as well as 20 eyes of 10 age-and-sex-matched

non
infected subjects were obtained. Protein normalization in

each sample was performed
by Lowry method; 1 g/ ml was

submitted to determine MUC2 and MUC5AC analysis by
ELISA technique. Inflammatory cytokines were determined by cytometric bead arrays.
Results:All patients with clinical adenovirus infection were confirmed

by PCR analysis
for adenovirus type 8. An average of one hundred

microliters was obtained from all tear
samples. MUC2 was significantly higher in infected samples

0.246 ( 0.2) versus non-
infected eyes 0.065 (

0.01) (p<0.001). TNF-a was 3.73 times increased in tears
samples of adenovirus patients when compared to healthy controls (p=0.02)
Conclusions:Up-regulation of MUC2 was observed in patients with adenovirus

infection.
It is possible that the proinflammatory microenvironment

enriched with TNF-a could
increase MUC2 expression.

156
I-11 RELEVANCE OF PROTEIN GLYCOSYLATION PATHWAYS FOR IMMUNE
SENSING OF Candida guilliermondii.
Navarro-Arias Mara de Jess
1
, Lpez-Prez Mercedes Guadalupe
2
, Mora-Montes Hctor Manuel
1,
*.
1
Departamento de Biologa, Divisin de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad
de Guanajuato, Noria Alta s/n, Col. Noria Alta, C.P. 36050, Guanajuato, Gto., Mxico.
2
Departmento de
Biotecnologa y Bioqumica, CINVESTAV-Irapuato, C.P. 36821, Irapuato, Gto., Mxico. * Corresponding
author: tel 473 732 0006, ext. 8154; email: hmora@ugto.mx

Candida guilliermondii is an opportunistic fungal pathogen usually associated with
immunocompromised patients, is considered less virulent than C. albicans but is one of
the main emergent species of this genus. The cell wall is the first site of interaction
between the fungi and host cells, so this structure is involved in triggering the immune
defense system. The composition and organization of C. guilliermondii cell wall is so far
unknown, but it is assumed that it is similar to that found in C. albicans; where it is
composed by an inner layer of chitin and -glucans, covered with an outer layer of
highly glycosylated proteins (N- and O- mannans). Mannans play important roles in cell
wall integrity, adhesion to host tissues, virulence and during the establishment of a
protective host immune response. The aim of this work is to elucidate the composition
and organization of C. guilliermondii cell wall and its role during immune sensing by
human monocytes. Where the results indicated that the fungus-immune cell interaction:
C. guilliermondii is more recognized that C. albicans this may be due to the morphology,
since it does not form hyphae C. guilliermondii or the composition and organization of
the cell wall may vary with respect to C. albicans and therefore recognition (experiments
to be done).


I-12 INHIBITORY EFFECT OF GALECTIN-1 OVER IL-6 EXPRESSION IN LPS-
TREATED DECIDUAL CELLS BY REDUCING IB. Gmez Chvez F 1, Castro Leyva
V 2, Estrada Gutierrez G 2, Cancino Daz J C 1, Cancino Daz M E 1, Rodrguez
Martnez S 1. Department of Immunology, Escuela Nacional de Ciencias Biolgicas,
Instituto Politcnico Nacional 1, Prolongacin de Carpio y Plan de Ayala s/n, C.P.

157
11340, Tel 57296000 ext 62498. Department of Immunobiochemistry, Instituto Nacional
de Perinatologa Isidro Espinosa de los Reyes 2, Montes Urales 800, C.P. 11000, Tel
55209900 ext 375.
sandrarodm@yahoo.com.mx
Abstract. Pregnancy is a complex process where several physiological pathways
interact. A down-regulated inflammatory response and the abundance of anti-
inflammatory molecules during gestation may explain the non-recognition of the fetus as
foreign tissue, and the lack of immune response against it. NF-B is a key regulator of
inflammatory gene transcription such as IL-6, IL-8, IL-1, and TNF-. Low activity of this
transcription factor is associated with the beneficial anti-inflammatory environment
during fetus development until delivery. However, NF-B activity may eventually
increase, up regulating pro-inflammatory molecules at certain pregnancy stages or
conditions. The increased production of pro-inflammatory cytokines could be
pathological during fetus development in pregnancy. On the other hand, Galectin-1
(Gal-1) is a glycan binding protein like lectin that is able to down-regulates inflammation.
It has been shown that Gal-1 is abundantly expressed at the feto-maternal interface in
humans, where it promotes maternal immune tolerance to the fetal semi-allograft. Gal-1
tolerance promotion mechanisms have been established over adaptive immune cells
like T cells and dendritic cells. Nevertheless, it has not been established the role of this
lectin in non-immune cells at the feto-maternal interface. Here, we determined that Gal-
1 is able to negatively regulate IL-6 expression in LPS-treated human decidual cells.
Our results show that Gal-1 acts down regulating IB expression, a recently described
NF-B activity promoter involved in the IL-6 gene transcription.
Methods. Isolation and Culture of Decidual Cells. Fetal membranes were collected from
women who delivered by cesarean section. Decidua was scraped from the chorion and
digested with collagenase (200 U/mg) and DNase (6.25 U/mL). Decidual cells were
obtained from a (60:50:40:20) discontinuous Percoll gradient and cultured with 1%
antibiotic-antimycotic and 10% FBS at 37C in 5% CO
2
. Gal-1 and LPS treatment.
Decidual cells were pre-incubated with or without Gal-1 (100ng/mL) and then stimulated
or not with LPS at the final concentration of 100 ng/mL for 2 h. RT-qPCR. RNA was
extracted using Trizol (Invitrogen). One g of total RNA was used to generate cDNA to
perform real time PCR reactions using taqman gene expression assays for IB
(Hs00230071_m1), IL-6 (Hs00985639_m1), IBNS (Hs01076336_m1), IB
(Hs00355671_g1) and endogenous Rplp0 (Hs99999902_m1) using a Step One Applied
Biosystems thermocycler. Gene expression was analysed using Ct method.
Results. Gal-1 down-regulates LPS-induced IL-6 and IB expression but did not show
effect over IBNS or IB.
Conclusions. Gal-1 might act at the feto-maternal interface promoting an anti-
inflammatory environment by reducing the expression of IB, a known promoter of the
activity of NFB.

158

O-01 INDUCED EXPRESSION OF N-GLYCOLYLNEURAMINIC ACID IN B16
MELANOMA CELLS, MODULATES CAVEOLIN-1 AND INTEGRIN 5 EXPRESSION
AND PROMOTES MESENCHYMAL-LIKE MORPHOLOGY.
Segatori Valeria Ins, Albert Marina, Gomez Daniel Eduardo, Alonso Daniel Fernando, Gabri Mariano
Rolando. Laboratorio de Oncologa Molecular, Universidad Nacional de Quilmes, Buenos Aires,
Argentina. Roque Saenz Pea 352. CP B1876BXD, Tel +54-11-4365-7100. mrgabri@unq.edu.ar.

Abstract.Caveolin-1 was recently proposed as an antimetastatic marker in melanoma
since it is downregulated in malignant phenotypes. In addition, its overexpression
reduces the presence of integrin in the highly metastatic B16F10 melanoma cell line.
Participation of glycosphingolipids seems to be important in caveolin-1 signaling
complex function. In mammalian cells, most prominent sialic acids are N-
Acetylneuraminic acid (NAc) and N-Glycolylneuraminic acid (NGc), usually found as
terminal constituents of different membrane glycoconjugates such as the GM3
ganglioside (NacGM3 or NGcGM3). Since NGcGM3 is overexpressed in several
malignant cancers, we evaluated the participation of GM3 gangliosides in the
development of the malignant phenotype. In this regard, we have cloned and
transfected B16F0 -low metastatic melanoma cell line- with the mRNA sequence of the
cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH), the enzyme that
catalyzes NGc synthesis. The resulting cell line was designated B16-H. In these cells,
we observed a dramatic down-regulation of the plasma membrane expression of
NacGM3 -highly expressed in B16F0 cells- and an increase in the expression of
NGcGM3. The presence of NGcGM3 in B16-H cells promotes in vitro cell proliferation
and adhesion. We observed that B16-H cells present reduced levels of caveolin-1 in
plasma membrane and a positive regulation of Integrin 5. Also, in vitro cultured B16-H
TRABAJOS ORALES (O)

159
cells showed substantial morphological changes from parental B16F0 cells, presenting
a spindle type morphology and a decrease in membrane protrusion number. Our results
suggest that GM3 ganglioside variants participate in the balance between caveolin and
integrin cell membrane expression in the development of malignant phenotype.

O-02 PROCEDURE TO OBTAIN IMMUNOGENIC CONJUGATES FROM THE
CAPSULAR POLYSACCHARIDES OF SEROTYPES 5, 14 AND 18C OF Streptococcus
pneumoniae TO TETANUS TOXOID.
Villar Aneiros A.
1
, Ramirez Gonzlez U.
2
, Chang Caldern J.
1
, Pedroso Fernndez J.
1
, Fernndez
Villalobos A.
1
, Garrido Arteaga R.
1
, Rodrguez Noda L.
1
, Santana Fernndez D.
1
, Valds Balbn Y.
1
,
Fernndez Santana V.
1*
, Vrez Bencomo V.
1
1
Center for Biomolecular Chemistry, Playa, Havana, Cuba.
2
Finlay Institute. Research-Production Center of Vaccines, La Lisa, Havana, Cuba.
annette.villar@cqb.cu

The urgent need for pneumococcal conjugate vaccines requires the incorporation of
new manufacturers who are facing highly complex technology since the entire
conjugation process can not affect the structural features for which the polysaccharide
is immunologically recognized. This paper develops a methodology for obtaining
immunogenic conjugates from the capsular polysaccharide serotypes 5, 14 and 18C
Streptococcus pneumoniae to tetanus toxoid as carrier protein. The capsular
polysaccharides were fragmented by acid hydrolysis and then oxidized with sodium
periodate. The modified polysaccharides were covalently conjugated to tetanus toxoid
by reductive amination method. The products obtained were quantified by colorimetric
techniques and characterized by size exclusion chromatography and proton nuclear
magnetic resonance. The conjugates obtained were evaluated from the immunological
point of view. As a result we established a technology for obtaining immunogenic
conjugates that allows performing stability studies and clinical investigation.

*
Dedicated to her memory, deceased 20
th
November 2011.

160

O-03 AN HEPTAVALENT CONJUGATE VACCINE AGAINST THE LATINOAMERICAN
MOST PREVALENT SEROTYPES OF Streptococcus pneumoniae.
Valds Balbn, Y.; Santana Medero, D.; Garca-Rivera, D.; Moreno Paredes, B.; Chang Caldern, J.; Cardoso San
Jorge, F.; Garrido Arteaga, R.; Villar Aneiros, A. ; Rodrguez Noda, L.; Fernndez Santana, V
1
.;Vrez Bencomo, V.
yury.valdes@cqb.cu. Center for Biomolecular Chemistry, Havana, Cuba.

A new conjugate vaccine containing the seven serotypes of S. pneumoniae more frequently
associated with infection in Latina-American was designed for its introduction in Cuba and other
countries of the region. The vaccine candidate contains each capsular polysaccharide 1, 5, 6B,
14, 18C, 19F and 23F conjugated to tetanus toxoid and aluminum phosphate as adjuvant. The
glycoconjugates were generated using a general procedure that conserved the molecular feature
needed for adequate recognition. The process involves 3 steps: i) fragmentation of capsular
polysaccharide by acid hydrolysis, ii) activation by peryodic oxidation and iii) conjugation to a
carrier protein by reductive amination.
The preclinical evaluation was realized in New Zealand rabbits; prove of memory by a booster
with the unconjugated CPS after 4 months and opsonophagocytic activity of serum antibodies.
The results proved that the vaccine elicited high titer of total IgG with high avidity and
specificity to the CPS after the two doses. Antibodies were functional as shown by their
opsonophagocytic titer over 1:8 for each serotype. On the other hand, safety was assessed by
several toxicological studies in rats, demonstrating that the vaccine candidate doesnt induce any
sign of unexpected toxicity
The clinical evaluation started evaluating the security in healthy adults using PSV23 as control
vaccine. The results of the phase I clinical trial in adults demonstrated that the conjugated
vaccine candidate is safe and able to activate the immune system.
In conclusion, the first Latin-American conjugate vaccine candidate against S. pneumonia is
immunogenic and safe in laboratory animals and adults

Dedicated to her memory. Deceased 20
th
November 2011

161

O-04 USE OF fkp GENE FROM Bacteroides fragilis TO BOOST THE SYNTHESIS OF
FUCOSYLATED OLIGOSACCHARIDES IN E. coli.
Arteaga-Cabello F.J 1; Arciniega-Fuentes M.T. 1; Newburg D.S. 2; Ruiz-Palacios G.M 1. National Institute
of Medical Sciences and Nutrition. Mxico City, Vasco de Quiroga 15, 14000. +52(55)56559675, 1.
Boston College, Chestnut Hill, MA; 2. ferartc@gmail.com

Recently, there has been a growing interest in fucosylated oligosaccharides. The main
drawback to their synthesis in E. coli has been the low intracellular levels of GDP-
fucose used by the fucosyltransferases, producing unacceptable yields. However
excellent yields have been obtained by modifying the de novo pathway to synthesize
GDP-fucose. Here we propose an alternative route to synthesize GDP-fucose, the
salvage pathway reported in Bacteroides fragilis, similar to the salvage pathway from
mammalians. An E. coli unable to use the D-lactose and L-fucose (LacZ
-
and FucI
-
) as
sources of energy was subcloned with genes futC (1,2-fucosyltransferase) from H.
pylori, fkp (bifunctional enzyme fucokinase/L-fucose-1-P-guanylyltransferase) from B.
fragilis, and fucR (transcriptional activator of the fuc operon). Using this construct, we
standardized a high-cell density fermentation process, where 2-FL and
Lactodifucotetraose (LDFT) were synthesized (confirmed by NMR spectroscopy) at
concentrations up to 10 g/L and 0.5 g/L, respectively, starting from concentrations of 10
g/L lactose and 5 g/L of L-fucose, with complete consumption of lactose. By modifying
the initial concentrations of these sugars, we improved the ratio 2-FL:LDFT up to 5:1.
Although the production of LDFT as a byproduct was unexpected, contrary to being a
problem, it adds value to the process because with 2-FL, LDFT is one of the major
fucosylated oligosaccharide constituents of milk from secretor mothers. This process is
highly efficient and possible to escalate for the massive production of fucosylated
oligosaccharides for clinical use.

162
O-05 TRANSCRIPTIONAL REGULATION OF 1,3 GALACTOSYLTRANSFERASE
3Gal-T5: ROLE OF HEPATOCYTE NUCLEAR FACTOR HNF1.
Zulueta Aida
1
, Caretti Anna
1
, Signorelli Paola
1
, DallOlio Fabio
2
, Trinchera Marco
3
.
1
Department of Health
Sciences, San Paolo Hospital, University of Milan;
2
Department of Experimental, Clinical and Specialty
Medicine (DIMES), University of Bologna;
3
Department of Medicine Clinical and Experimental, University
of Insubria Medical School, Varese, Italy
Department of Health Sciences, San Paolo Hospital, University of Milan, via A. di Rudin 8, 20142 Milan,
Italy. Tel (+39)50323262 aida.zulueta@unimi.it; aidazulueta@gmail.com

1,3 galactosyltransferase 3Gal-T5 is responsible for the synthesis of type 1 chain
oligosaccharides, including Lewis antigens Lewis a, Lewis b, and sialyl-Lewis a, the
epitope of tumor marker CA19.9 and an E-selectin ligand potentially involved in cancer
metastasis and malignancy. 3Gal-T5 transcription occurs through two main promoters.
In the mammary gland, thymus, and trachea, as well as in some human cancer cell
lines, it is driven by a native promoter that is epigenetically modulated and sensitive to
nuclear factor NF-Y. In the organs of the gastrointestinal tract (as the colon, stomach
and pancreas) another stronger promoter is active and named the LTR promoter after
its retroviral origin. It is supposed to be regulated through homeoproteins such as
hepatocyte nuclear factor HNF1 and caudal-related homeobox Cdx. Surprisingly,
3Gal-T5 is strongly down regulated in cancer and our long-term objective is to
understand why and through what mechanism it happens. To this aim we determined
the expression levels of putative transcription factors by western blot and the amounts
of 3Gal-T5 transcripts by competitive RT-PCR in cancer tissues and cell lines.
Moreover, we silenced HNF1 or in two different cell lines, through a shRNA
approach, and expressed them in another by permanent cDNA transfection. Our results
suggest that HNF1 and HNF1 are necessary but not sufficient to drive expression of
LTR promoter: they play an interchangeable and not cumulative role and are not
immediately responsible for cancer down-regulation. Conversely, the role of Cdx1/2
appeared negligible suggesting that other unknown factors are involved.

163

O-06 A CROSSTALK BETWEEN O-GLCNACYLATION, PHOSPHORYLATION,
UBIQUITINATION AND SUMOYLATION CONTROLS THE TRANSCRIPTIONAL
ACTIVITY OF DELTA-LACTOFERRIN
Escobar-Ramirez Adelma, Huvent Isabelle, Hoedt Esthelle, Dehnnaut V and Pierce Annick
Unit de Glycobiologie Structurale et Fonctionnelle, UMR 8576 CNRS-Universit des Sciences et
Technologies de Lille, IFR 148, 59655 Villeneuve d'Ascq, France

Deltalactoferrin (Lf) is a transcription factor the expression of which is downregulated
in cancer. It is a healthy tissue marker and a high expression level of its transcripts was
correlated with a good prognosis in breast cancer. Lf results from alternative promoter
usage. Alternative lactoferrin gene promoter usage leads to the production of two
isoforms with alternative N-termini: lactoferrin, which is secreted, and Lf, its
nucleocytoplasmic counterpart. Lf possesses anti-proliferative effects and induces cell
cycle arrest. It is an efficient transcription factor interacting in vivo via
element found in the Skp1, Bax, DcpS and SelH promoters. Since Lf possesses
different target genes, modifications in its activity or concentration may have crucial
incidences on cell homeostasis. Posttranslational modifications efficiently modulate
transcription factor activity. Our earlier investigations showed that O-GlcNAcylation
negatively regulates Lf transcriptional activity while it inhibits its ubiquitination and
increases its half-live. On the other hand, phosphorylation activates Lf transcriptional
activity. Recently we showed that Lf is also modified by SUMOylation. SUMO stands
for Small Ubiquitin-like MOdifier is an Ub family member. SUMOylation is a
widespread mechanism for rapid and reversible changes in protein function.
SUMOylation targets mainly nuclear factors and regulates numerous cellular processes
such as nuclear transport, transcriptional activity and DNA repair. In most cases
SUMOylation triggers transcriptional repression. We screened Lf primary structure for
the presence of SUMO sites and four putative SUMO consensus sequences
(SCIK
13
RDSP, NLRK
308
SEE, LVLK
361
GEA, ENYK
391
SQQ) were found. We are currently

164
mapping the SUMO sites and studying the role of SUMOylation on Lf transcriptional
activity and stability. Our preliminary results showed that the four sites are SUMOylated
and invalidation of the four SUMO sites leads to an increased transcriptional activity
suggesting that SUMOylation represses Lf activity.
Transcription factors are often regulated by combinations of different PTMs which might
act as a molecular barcode. We are currently investigating the crosstalk between them
in order to decrypt the Lf code responsible for the control of its activity. SUMO
usually competes with ubiquitination and phosphorylation. Ub/SUMO are mutually
exclusive whereas SUMO/phosphorylation can be agonistic or antagonistic modification
depending of substrates. Interestingly, among the four SUMO sites, two are located in
areas which are targeted by other modifications. At the N-terminus the O-
GlcNAcylation/phosphorylation site is adjacent to the SUMO-1 site. Therefore the two
regulatory sites are in close proximity. The crosstalk between these sites could
constitute the Lf code responsible of the control of the transactivation of Lf target
genes. Now, at the C-terminus SUMO-4 site is in the heart of the PEST sequence.
SUMO is known to compete with ubiquitination therefore K391 is either ubiquitinated or
sumoylated. So, in that way SUMOylation would positively regulate stability. Therefore
at the PEST motif a crosstalk between phosphorylation/SUMOylation/ ubiquitination
could constitute the Lf code responsible of the control of Lf stability. These
agonistic and/or antagonistic modifications may contribute to the establishment of finely
regulated mechanisms of Lf transcriptional activity and stability depending on the type
of target genes and cellular homeostasis.

O-07 DEVELOPMENT OF SINGLE CHAIN FV ANTIBODY FRAGMENT AGAINST -
GAL EPITOPE WITH POTENCIAL USE FOR IN SITU DIAGNOSIS OF HUMAN
PAPILLOMAVIRUS.
Manzo Sandoval A. 1, Gavilondo Cowley J.V. 2, Medina Escutia M.E. 3, Medina Flores Y. 3, Ramn
Gallegos E. 1. Escuela Nacional de Ciencias Biolgicas (IPN), Av. Wilfrido Massieu Esq. Cda. Miguel
Stampa s/n C.P. 07738, Mxico D.F. Tel. 57 29 60 00 ext. 52399 (1). Centro de Ingeniera Gentica y
Biotecnologa (CIGB), Ave 31 e/ 158 y 190, Playa, P.O. Box 6162, La Habana 10600, Cuba. Tel. 53-7-

165
2718008 (2). Instituto de Diagnstico y Referencia Epidemiolgicos (InDRE), Prolongacin de Carpio 470
Col. Casco de Santo Tomas C.P. 11340, Mxico D.F. Tel. 53 42 75 50 ext. 260 (3). eramong@ipn.mx

The -Gal epitope is an oligosaccharide component of glycolipids and glycoproteins
displayed on the cell surface of non-primate mammals but not in humans. However, -
Gal is present in cervical mucus, koilocytes and keratinocytes from pacients with
cervical cancer and human paillomavirus (HPV). Our working group generated an anti
-Gal specific IgM monoclonal antibody (mAb) which recognizes cells infected with
HPV-16 (cervical cancer biopsy sections) and HPV-18 (HeLa). The objective of this
work is to obtain a single chain Fv (scFv) from anti -Gal mAb, to be used in
colposcopic diagnosis. Primers were designed to amplify the light (VL) and heavy (VH)
mAb variable regions. Total RNA was extracted from hybridoma ATCC PTA-13359 that
produces anti -Gal specific mAb, cDNA was synthesized and PCR was standardized
for VH and VL amplification. The PCR products were sequenced and were design new
primers to be used in an overlapping PCR technique, for the insertion of a short linker or
long linker between VH and VL. It was determined that VL is constituted by 318 pb and
VH by 363 pb. It were obtained two products of 700 pb by overlapping. Monobodies and
diabodies generated will be analyzed in the BIACORE system and will be tested, in
positive HPV cell lines 16 and 18 and in cervical cancer biopsy sections. It were
constructed two types of scFvs and currently we are working on the expression of these
fragments that can be used in vivo diagnosis of HPV.

O-08 POSSIBLE INVOLVEMENT OF TUMOR-ASSOCIATED LEUKOSIALIN CD43 IN
EPITHELIAL-MESENCHYMAL TRANSITION.
S-Diniz JN, Valente RC, Takiya CM, Mendona-Previato L, Previato JO, Freire-de-Lima L. Laboratrio
de Glicobiologia, Instituto de Biofsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio
de Janeiro, Brasil. Tel. (21) 2562-6646. previato@biof.ufrj.br

166
Introduction: Epithelial-mesenchymal transition (EMT) plays critical roles in chronic
diseases such as cancer. Changes in protein glycosylation are striking features of
transformed cells and are directly related to tumor malignancy. Studies have
demonstrated that CD43, which is normally expressed on the surface of leukocytes is
also expressed in carcinomas. However, the role of CD43 in tumor development and
progression is still unknown. Objectives: The current study aims to evaluate the level of
expression of CD43 during EMT, as well as to identify the metabolic pathways that may
be modulated by this signaling mucin. Material and Methods: A549 cell line was
maintained in RPMI supplemented with 10% fetal bovine serum at 37C/5% CO2. The
EMT was induced by TGF or hypoxia (N2
94
%/ CO2
5
%/O
2
1%). The expression of
epithelial and mesenchymal markers, as well as, CD43 were determined by Western
blot. Cell motility was monitored using phagokinetic assay, and the morphological
changes were quantified with the aid of the Image J program. Results: Cells treated with
TGF or maintained in hypoxia showed fibroblastic morphology, alterations in the
expression of the epithelial and mesenchymal markers, as well as increased cell motility
when compared to control cells. In addition, the cells exposed to hypoxia showed
inscreased expression of CD43, as well as the transcription factor Twist. Conclusion:
Since CD43 plays important roles in leukocytes biology it is reasonable to speculate that
the increased expression of CD43 in carcinoma cells undergoing EMT may confer a
selective advantage to the invasive and migratory cells during tumor dissemination,
which would render the CD43 as a promising target in cancer therapy.

O-09 Cryptococcus neoformans MANNOPROTEINS ADHESIVE PROPERTIES.
Teixeira PAC, Mendona-Previato L, Ferreira IC and Previato JO. Laboratrio de Glicobiologia, Instituto
de Biofsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil. Tel.
(21) 2562-6646. luciamp@biof.ufrj.br

Introduction: Cryptococcus neoformans is an oportunistic fungal pathogen, causative
agent of cryptococcosis. The disease kills about 630,000 people per year around the

167
world. The major determinant of virulence of this fungus is the capsule production. The
capsular network consists of highly hydrated polysaccharides, including
glucuronoxylomannan (GXM), the most abundant component of the capsule, and
glucuronoxylomannogalactan (GXMGal). C. neoformans capsule is also composed by
mannoproteins (MPs), which corresponds of less than 1 % of the capsular weight.
Considering that during the infection, the MPs, as the other capsule components, are
the first substrate to contact the host tissues, we aimed to study the role of these
glycomolecules in the interaction of C. neoformans with host cells.
Methodology: We used a wild type C. neoformans strain (NE-241) and an acapsular
mutant strain (CAP67) throughout this work. The capsule constituents GXM, GXMGal
and a recombinant mannoprotein MP84 were previously purified in our laboratory. We
performed interaction assays of the fungus to epithelial lung cells (A549), in the
presence or not of capsule constituents (GXM, GXMGal e MP84), during 1 hour at 37
C.
Results: We observed that encapsulated (NE-241) and acapsular (CAP67) strains were
able to adhere to A549 cells, and that GXM inhibits the NE-241 adhesion and not
CAP67. In contrast, CAP67 adhesion was only inhibited in the presence of MP84. The
binding of MP84 to A549 cells was observed by ELISA and immunofluorescense. The
results suggest that MPs are involved in the adhesion of C. neoformans to lung
epithelial cells.

O-10 THE THERAPEUTIC EFFECT OF IMMUNOGLOBULIN IN MURINE
TUBERCULOSIS IS DEPENDENT ON IgG GLYCOSYLATION
Olivares- Arzuaga N 1.2, Marquina B 1, Mata- Espinoasa D 1, Zatarain-Barron ZL 1, Espitia-Pinzn C 2,
Estrada I 3, Collin M 4, Rook G 5and Hernandez-Pando R1.
Departamento de Patologa Experimental, INCMNSZ 1. Investigaciones Biomdicas, UNAM 2.
Departamento de Inmunologa, IPN 3. Department of Clinical Sciences, Lund University 4.

Centre for
Clinical Microbiology, University College London 5. INCMNSZ Vasco de Quiroga 15, Colonia Seccin
XVI, Delegacin Tlalpan, Mexico, DF. CP 14000, rhpando@hotmail.com

168

Abstract. Introduction: antibodies have demonstrated having a therapeutic effect in
animal models of tuberculosis. These experiments have considered the specificity of
antigen recognition and their different classes as significant contributors of this effect.
However, the carbohydrate chain heterogeneity on the Fc region of IgG (Fc-IgG) can
play an important role modulating the immune response. Tuberculosis patients usually
have high titers of specific IgG, however the carbohydrate associated to Fc-IgG usually
lacks galactose. Material and Method: the specificity of recognition against proteins from
M. tuberculosis of human intravenous immunoglobulin (intact IVIg) and IVIg treated with
endoglycosidase S (EndoS-treated IVIg) which hydrolyzes the glycan on Asparagine-
297 in the Fc-IgG, was made. Kinetics of distribution of IVIg administrated by
intraperitoneal (i.p.) rout, in serum and lung lavage of mice were determinates. Finally a
challenge experiment was performed: mice were infected with MTB H37Rv
intratracheally; different groups of animals were treated intraperitonially on days 3 and 5
with intact IVIg, EndoS-treated IVIg or saline solution. Results: equal recognition against
proteins of intact IVIg and EndoS-treated IVIg. IVIg administered by i.p. route reaches
the bronchial tree during the first 24 hours post-inoculation, and was detected in serum
for at least 21 days. Intact IVIg treatment caused a striking reduction in lung colony
forming units on day 14 and 28, and also increased the area of granulomas and
reduced pneumonia. This effect was totally abrogated by EndoS treated-IVIg.
Conclusion: IVIg has a therapeutic effect in a mouse model of TB, but this effect
requires intact Fc oligosaccharides.

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