SUBJECT: BIOLOGY 1 SUBJECT CODE: FIS0414 TIME ALLOWED: 3 HOURS (2.00 PM !.00 PM" DATE: APRIL 24# 2012 T$%& '()*%+),%-+ .--/0', 1-+,)%+& TWENTY THIRD (23" 23%+,'4 2)5'& %+1064%+5 ,$' 1-7'3 2)5'. INSTRUCTIONS TO CANDIDATE: 1. Answer ALL questions. 2. There are FORTY (40" questions in SECTION A. 3. There are SIX (8" questions in SECTION B. INFORMATION TO CANDIDATE: 1. You are permitted to use a non-programmable electronic calculator in this examination. SECTION A A+&9'3 ALL :6'&,%-+& 1. When using a light microscope, magniication o the structure being obser!ed can be increased b" a !ariation in the A. light intensit". #. stage position. $. ob%ecti!e lens power. &. iris diaphragm setting. R';'3 ,- ,$' ;-00-9%+5 4%)53)* -; ) 1'00 ,- )+&9'3 :6'&,%-+ 2. 2. A protein being pac'aged and modiied or export would be ound in A. W #. ( $. Y &. ) 2 3. Which o the ollowing t"pes o molecules are s"nthesi*ed in the rough endoplasmic reticulum+ A. lipids #. proteins $. nucleic acids &. carboh"drates R';'3 ,- ,$' ;-00-9%+5 4%)53)* -; ) 1'00 ,- )+&9'3 :6'&,%-+ 4. ,. #ased on the internal structure, the cell is li'el" to A. be a chee' epithelial cell. #. be a mature red blood cell. $. require high le!els o ox"gen. &. produce high le!els o sugars. -. The organelle which contains inormation or the production o protein in a cell is A. the nucleus. #. the nucleolus. $. the .olgi bod". &. the smooth endoplasmic reticulum.
R';'3 ,- ,$' ;-00-9%+5 4%)53)* -; ) 1'00 -35)+'00' ,- )+&9'3 :6'&,%-+ 8. 3 /. 0ne unction o structure X is to A. replicate &1A. #. produce am"lase. $. transcribe m21A. &. h"drol"*e nuclease. R';'3 ,- ,$' ;-00-9%+5 4%)53)* -; ) 1'00 *'*.3)+' ,- )+&9'3 :6'&,%-+ <. 3. 4 a substance temporaril" intereres with the unction o the structure labelled X, which o the ollowing would occur+ A. The brea'down o worn-out cell organelles would stop. #. There would be an increase in the production o m21A. $. The number o amino acid molecules entering the cell would decrease. &. The acti!e transport o water across the cell membrane would decrease. R';'3 ,- ,$' 4%)53)* -; ) 9),'3 *-0'160' .'0-9 ,- )+&9'3 =6'&,%-+ >. , 5. The polarit" o the molecule abo!e accounts or which o the ollowing characteristics+ A. An acidic p6 #. 7ow densit" as a liquid $. 4ts abilit" to act as a sol!ent &. 4ts abilit" to buer reactions T$' 4%)53)* %& ) *-4'0 -; )+ %*2-3,)+, .%-0-5%1)0 23-1'&&. 8. What prediction can be made using the model+ A. 9alt ions mo!e b" osmosis into an area o low solute concentration rom an area o high solute concentration. #. 9alt ions mo!e b" diusion rom an area o high solute concentration to an area o low solute concentration. $. Water molecules mo!e b" diusion rom an area o high solute concentration to an area o low solute concentration. &. Water molecules mo!e b" osmosis rom an area o low solute concentration to an area o high solute concentration. 1:. A characteristic o unsaturated att" acids is that the" - A. are made up o gl"cerol. #. are pol"mers o glucose. $. are pol"mers o nucleotides. &. ha!e double bonds between some carbon atoms. 11. The ma%or component o a plant cell wall is a product ormed rom the deh"dration s"nthesis o A. att" acids #. nucleotides $. amino acids &. monosaccharides 12. 6ow are starch and cellulose similar+ A. #oth are pol"mers o glucose. #. #oth are produced in human cells. $. #oth are components o the cell membrane. &. #oth can be digested b" en*"mes produced in the pancreas. 13. ;rimar" protein structure is dependent on A. peptide bonding. #. h"drogen bonding. $. bonding between 2-groups. &. bonds between protein molecules. 1,. .l"cogen is A. a pol"saccharide ound in animal cells. #. an energ"-storing lipid molecule. $. a molecule in which plants store sugars. &. a pol"saccharide ound in plant cell walls. 1-. Two water molecules are produced b" the union o glucose molecules during deh"dration s"nthesis. The new molecule ormed is a A. dipeptide. #. disaccharide. $. pol"saccharide. &. monosaccharide. 1/. $arboh"drates, lipids, proteins and nucleic acids are similar because the" A. act as catal"sts. / #. are made up o carbon atoms. $. ha!e a tertiar" shape held together b" h"drogen bonds. &. orm part o the phospholipid bila"er in the cell membrane. 13. <xperiments were carried out to show the eects o p6 and temperature on en*"me acti!it". The experiments also tested the eects o a chemical called an inhibitor. The results are shown in the graphs. The best conclusion that can be drawn rom these results is that the inhibitor aects A. p6. #. temperature. $. en*"me acti!it". &. en*"me concentration.
15. <n*"mes increase the rate o a reaction b" A. producing energ". #. becoming denatured. $. producing coen*"mes. &. decreasing the energ" o acti!ation. 18. =itamins unction as 3 A. en*"mes. #. coen*"mes. $. emulsiiers. &. competiti!e inhibitors. 2:. Which o the ollowing is +-, a part o the theor" o how an en*"me unctions+ A. The shape o the acti!e site acilitates a reaction. #. The en*"me and substrate it li'e a loc' and 'e". $. The en*"me lowers the acti!ation energ" required or the reaction. &. The shape o the en*"me is permanentl" changed b" the chemical reaction. R';'3 ,- ,$' ;-00-9%+5 4%)53)* ,- )+&9'3 :6'&,%-+ 21. METABOLIC PATHWAY 21. 4 substance ? inhibits en*"me 2, this will in turn inhibit the production o A. en*"me 1. #. en*"me 2. $. substance W. &. substance Y. R';'3 ,- ,$' ;-00-9%+5 4%)53)* -; )+ '+@A*' &6.&,3),' %+,'3)1,%-+ ,- )+&9'3 :6'&,%-+ 22. 5 22. The structure labelled ( in the reaction abo!e is A. product. #. en*"me. $. substrate. &. en*"me-substrate complex. R';'3 ,- ,$' 4%)53)* -+ '+@A*' )1,%7%,A .'0-9 ,- )+&9'3 :6'&,%-+ 23 23. Which one o the graphs abo!e represents the data rom an experiment exploring the eect o p6 on the acti!it" o pepsin+ A. W #. ( $. Y &. ) 2,. The ma%orit" o AT; is produced b" A. gl"col"sis. #. the >rebs c"cle. $. the respirator" chain. &. $02 reducing reactions. 2-. The autotrophic acti!ities o plant cells occur in the 8 A. c"toplasm. #. chloroplast. $. ribosome. &. nucleus. R';'3 ,- ,$' ;-00-9%+5 4%)53)* -; ) 1$0-3-20)&, ,- )+&9'3 :6'&,%-+ 28. 2/. The area o the diagram in which 1A&; is produced is A. W #. ( $. Y &. ) 23. The most eecti!e wa!elengths o the light spectrum or photos"nthesis are A. red and "ellow. #. blue and "ellow. $. red and blue. &. blue and purple. 25. &uring the light-dependent reactions o plants the ollowing occurs A. electron transport. #. chemiosmosis. $. splitting o water. &. all o the abo!e. 28. &uring c"clic photophosphor"lation, 1: A. ox"gen is generated. #. water is split. $. AT; is produced. &. 1A&;6 is produced. 3:. Which one o the ollowing acts as an electron carrier in cellular respiration+ A. A&;. #. AT;. $. 0x"gen. &. 1A& ? 31. 7actic acid ermentation occurs in
A. bread dough. #. an" en!ironment containing ox"gen. $. muscle cells. &. mitochondria. 32. The process common to aerobic and anaerobic respiration is A. oxidation. #. gl"col"sis. $. >erb@s c"cle. &. electron transport chain. 33. Which o the ollowing shows &1A replication in the correct order+ A. Two &1A double helices strands separate matching bases pair up &1A double helix. #. &1A double helix strands separate matching bases pair up two &1A double helices. $. 9trands separate two &1A double helices matching bases pair up &1A double helix. &. &1A double helix strands separate two &1A double helices matching bases pair up. 3,. Which one o the ollowing statements correctl" describes &1A+ A. .uanine bonds to c"tosine and adenine bonds to uracil. #. ;eptide bonds are ound between guanine and c"tosine. $. 6"drogen bonds are ound between th"mine and guanine. &. <ach nucleotide is composed o a sugar, a phosphate and a base. 3-. The term codon is used to describe a sequence o bases in 11 A. 21A. #. r21A. $. t21A. &. m21A. R';'3 ,- ,$' ;-00-9%+5 4%)53)* ,- )+&9'3 :6'&,%-+& 38# 3< )+4 3>. 3/. The process which produces molecule ( is A. translation. #. replication. $. denaturation. &. transcription. 33. The t"pe o bond which %oins the structure in box Y with the structure in box ) is A. ionic. #. peptide. $. co!alent. &. h"drogen. 35. The two components which are part o the structure shown in box Y are A. ribose and guanine. #. ribose and c"tosine. 12 $. deox"ribose and uracil. &. deox"ribose and adenine. 38. Which one o the ollowing is the best deinition o recombinant &1A+ A. ;lasmids. #. #acterial &1A. $. &1A which has been cloned. &. &1A rom more than one source ,:. 4n a !ariet" o garden peas, the allele or tall plants ATB is dominant o!er the allele or short plants AtB. A cross between a tall plant and a short plant resulted in -:C o the ospring being short. What were the genot"pes o the parents+ A. Tt and tt #. Tt and Tt $. TT and Tt &. TT and tt (T-,)0: 40 *)3/&" SECTION B A+&9'3 ALL :6'&,%-+& =6'&,%-+ 1 aB $omplete T).0' 1 in "our answer paper ,- gi!e TWO (2" wa"s in which the structure o a plant cell dier rom that o a pro'ar"otic cell. (2 *)3/&" T).0' 1 13 P0)+, 1'00 P3-/)3A-,%1 1'00 1. 2.
AiiB the smallest percentage o nitrogen-containing substances in its seeds+ (1 *)3/" cB &escribe how "ou could test a #ra*il nut to show that it contained lipids. (3 *)3/&" T$' 2$-,-53)2$ &$-9& 1'00& ;3-* )+ -+%-+ 3--, ,%2. T$' 3--, ,%2 $)& .''+ &:6)&$'4 )+4 &,)%+'4 ,- &$-9 ,$' &,)5'& -; *%,-&%&. 1, dB AiB At what stage o mitosis is cell A+ (1 *)3/" AiiB What is the e!idence that cell B is in anaphase+ (1 *)3/"
AiiiB $ell C is in interphase. .i!e TWO (2" processes which occur during interphase that enable cell di!ision to occur. (2 *)3/&" eB <xplain how "ou would calculate the magniication o the photograph. (1 *)3/" (T -,)0: 12 *)3/&" =6'&,%-+ 2 1- T$' T).0' .'0-9 &$-9& ,$' 1-+1'+,3),%-+ -; &-*' 4%;;'3'+, 23-,'%+& )+4 '&&'+,%)0 )*%+- )1%4& %+ &)*20'& -; $6*)+ *%0/. AaB &escribe how "ou could use a biochemical test to show that a mil' sample contain protein. (2 *)3/&"
AbB At birth an inant@s intestine secretes relati!el" small amounts o digesti!e en*"mes. AiB <xplain the ad!antage o mil' containing lipase stimulated b" bile salts. (2 *)3/&" AiiB <xplain the ad!antage to the inant o the concentration o essential amino acids in the mil' sample 3 da"s ater birth. (2 *)3/&" T$' T).0' .'0-9 &$-9& ,$' ;),,A )1%4 1-+,'+, -; &)*20'& -; $6*)+ *%0/ -.,)%+'4 ;3-* 9-*'+ %+ ,$' U+%,'4 B%+54-* %+ 1C!3 )+4 1C<<. 1/ AcB &escribe and suggest an explanation or the dierence between the saturated att" acid contents o the 18-3 and 1833 samples. (2 *)3/&" AdB &escribe FOUR (4" wa"s that water is important to li!ing organisms. (4 *)3/&" (T-,)0: 12 *)3/&"
=6'&,%-+ 3 Am"lase is an en*"me. 4t catal"ses the reaction am"lase 13 starch maltose 9tudents mixed a starch solution with am"lase. The" recorded the concentration o maltose at inter!als or 3: minutes. The graph below shows their results. AaB &escribe how the concentration o maltose changed o!er the period shown in the graph abo!e. (2 *)3/&" AbB AiB A straight line has been drawn on the graph abo!e. <xplain how "ou could use this line to calculate the initial rate o the reaction. (1 *)3/" AiiB The rate o reaction was lower ater - minutes than it was at the start. <xplain wh". (2 *)3/&" AcB The graph below shows the eect o p6 on the acti!it" o am"lase. 15
9uggest the region o the human gut in which this am"lase would be acti!e. <xplain "our answer. (3 *)3/&" AdB 6ow would the shape o the graph below change i a large amount o concentrated acid were added to the en*"me-catal"*ed reaction at time X+ 4n "our answer paper 43)9 the change on the graph and explain "our answer.
(3 *)3/&" (T-,)0: 11 *)3/&" =6'&,%-+ 4 18 AaB The diagram summarises some o the light-dependent reactions o photos"nthesis. AiB Ese the diagram to describe what happens to a molecule o chloroph"ll in photos"stem 44 when it absorbs a photon o light. (2 *)3/&" AiiB Dolecules o AT; are ormed as electrons are transerred rom photos"stem 44 to photos"stem 4. <xplain how this is possible. (2 *)3/&" AbB 2educed 1A&; produced during the light-dependent reactions o photos"nthesis is used in the light-independent reactions. <xplain how. (2 *)3/&" (c) In your answer paper complete the following table contrasting photosynthesis and cellular respiration.
(T-,)0: 10 *)3/&" =6'&,%-+ ! AaB 1ucleic acids, such as &1A, are pol"mers, made up o man" repeating monomer units. 1ame the monomer rom which nucleic acids are made. (1 *)3/" 21 AbB The table shows the percentage o dierent bases in the &1A o some organisms. AiB $alculate the missing igures or rat &1A bases. (3 *)3/&" AiiB The !irus has single-stranded &1A as its genetic material. <xplain the e!idence rom the table which suggests that the &1A is single-stranded. (2 *)3/&" AcB 4n "our answer paper complete the table to gi!e TWO (2" wa"s in which the structure o &1A diers rom the structure o m21A (2 *)3/&" DNA *RNA
(T-,)0: > *)3/&" =6'&,%-+ 8 AaB AiB 9ome human &1A was cut into separate pieces using a restriction en*"me which produced a staggered cut. A scientist wanted to insert these pieces o &1A into plasmids and used the same restriction en*"me to cut the plasmids. 22 <xplain wh" the pieces o human &1A would be able to %oin to the cut &1A o the plasmids. (2 *)3/&"
AiiB Which other en*"me must the scientist ha!e added to the mixture to orm recombinant plasmids+ (1 *)3/" AbB A plasmid ma" be used as a !ector. <xplain what is meant b" a vector. (2 *)3/&"
AcB Dolecular biologists oten use plasmids which contain antibiotic resistance genes. <xplain the reason or this. (2 *)3/&"