Escolar Documentos
Profissional Documentos
Cultura Documentos
Bray, Ireland
September 16th, 2009
Resolution Software Cont.
Peak Labeling
Error Codes
1 = Wide A peak (the A peak in the sample is 1.3 times wider)
Possible causes: A peak and variant peak merging
Underconcentrated FASC
Overconcentrated patient samples
2 = Area of A <80%
3 = Peak area greater than expected (>5%)
4 = Peak is after A2
5 = A1c is greater than 10% (in order to confirm A1c or variant)
6 = HbF or variant is present
7 = peak area too small / big
Consequences: Total area of the chromatogram is not within specs
Will not be reflexed to HR
Will not be transmitted to LIS
8 = %A2 is not within the normal range
Hemoglobin F rules
• Normal range is less than 2%
• Specify the minimum %F to be labeled 0.5 – 5.0%
• There are 2 RRT windows for F, F>10% and F<10%
• If 14%<%F<35% the peak will be labeled with Code 6
Otherwise it will be “labeled consistent with F”
• Acetyl F will be labeled if 10% or more of %F
Normally it is 10 – 20% of F
Hemoglobin A rules
• If a peak in the A window is 15% or greater it will be labeled as A
• If <15% Code 3
• If <5% will be ignored
• If <80% Code 2
Hemoglobin A2 rules
• Normal range 1.5 – 3.0%
• If >0.1% and <10% labeled A2
• If >10% Code 3
• If less than or greater than expected Code 8
Genesys Software
Beta version (has not been released yet)
Only differences will be noted
Setup – Assay
There’s an option to print QS/HR side-by-side report after reflex. It only prints once after
reflex testing. Report cannot be regenerated.
Exception report can be generated.
The set-up of the micro-well titer plate one can indicate which positions to dilute or skip
in the option “Enter Dilution Template”. Red wells will not be sampled. Yellow wells
will not be diluted e.g. hemolysate control samples.
One can use either traditional round bottom plates or tall micro-well plates. Sampling
will start at position A-1. Reminder: The set-up of the micro-well titer plate is completely
customizable.
You can use tall micro-well titer plate for adult samples (5µ L EDTA blood and fill it up
to 1 mL) and run HR (the 2 min. QS on Genesys doesn’t identify/quantify A2 and F).
Hemoglobin Genetics
HbA α 2β 2
HbA2 α 2δ 2
HbF α 2γ 2
HbH β 4
Hb Bart’s γ 4