Results from Compound Optimization

Compound Optimization in MS and MS/MS

08:55:56: Optimizing flat device(s) using ion 329.07 m/z
08:55:57: Optimizing Spray Voltage for Q1MS
08:56:12: Previous Setting = 4000.00, New Setting = 3000.00
08:56:12: Maximum Intensity = 2.34e+01
08:56:12: Insignificant improvement, kept the previous setting
08:56:13: Optimizing Sheath Gas Pressure for Q1MS
08:56:33: Previous Setting = 5.00, New Setting = 10.00
08:56:33: Maximum Intensity = 1.43e+00
08:56:33: Insignificant improvement, kept the previous setting
08:56:33: Optimizing Ion Sweep Gas Pressure for Q1MS
08:57:08: Previous Setting = 0.00, New Setting = 6.00
08:57:08: Maximum Intensity = 1.44e+00
08:57:08: Insignificant improvement, kept the previous setting
08:57:08: Optimizing Aux Gas Pressure for Q1MS
08:57:33: Previous Setting = 0.00, New Setting = 15.00
08:57:33: Maximum Intensity = 3.86e+02
08:57:33: 1691 % Improvement
08:57:33: Optimizing Vaporizer Temperature for Q1MS
08:57:33: Heating Vaporizer Temperature up to 400 (C)
08:58:14: Collecting data......
08:59:00: Finished collecting data for Vaporizer Temperature
08:59:00: Previous Setting = 34.04, New Setting = 331.75
08:59:00: Maximum Intensity = 7.62e+04
08:59:00: 7179 % Improvement
08:59:00: Optimizing Capillary Temperature for Q1MS
08:59:01: Heating Capillary Temperature up to 400 (C)
09:01:20: Collecting data......
09:10:07: Finished collecting data for Capillary Temperature
09:10:07: Previous Setting = 270.28, New Setting = 281.46
09:10:07: Maximum Intensity = 7.79e+04
09:10:07: 23 % Improvement
09:10:08: Optimizing S-Lens RF Amplitude for ion 329.07 m/z
09:10:14: Previous Setting = 83.13, New Setting = 145.19
09:10:14: Maximum Intensity = 3.43e+04
09:10:14: 198 % Improvement
09:10:15: Old Parent Mass: 329.065, New Parent Mass: 329.132
09:10:15: Optimizing collision energy at 1.5 mTorr
09:10:15: Waiting for the collision gas to stabilize
09:10:36: Finding the product ions of ion 329.1 m/z
09:11:12: Constructing the breakdown curve of ion 329.1 m/z
09:11:14: Product Ion: 267.03 Maximum Intensity: 4.19e+04
09:11:16: Product Ion: 297.17 Maximum Intensity: 8.15e+04
09:11:18: Product Ion: 249.08 Maximum Intensity: 1.87e+04
09:11:20: Product Ion: 265.07 Maximum Intensity: 1.16e+04
09:11:20: Collision Energy Optimization Results:
09:11:20: Product Ions (m/z) Coll. Energy (v)
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09:11:20: 267.03 29
09:11:20: 297.17 14
09:11:20: 249.08 40
09:11:20: 265.07 22
09:11:21: Finish compound optimization
Comments:
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Optimizing Spray Voltage for Q1MS
3000 3500 4000 4500 5000
Spray Voltage Value (V)
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PreviousSetting=4000.00
Insignificant improvement,kept the previoussetting
Optimizing Vaporizer Temperature for Q1MS
200 250 300 350 400
Vaporizer Temperature (C)
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PreviousSetting=34.04
Optimum Setting=331.75
7179 %Improvement
Optimizing Sheath GasPressure for Q1MS
10 20 30 40 50 60
SheathGasPressure Value (psi)
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PreviousSetting=5.00
Insignificant improvement,kept the previoussetting
Optimizing Ion Sweep GasPressure for Q1MS
0 2 4 6 8
IonSweepGasPressure Value (psi)
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PreviousSetting=0.00
Insignificant improvement,kept the previoussetting
Optimizing Aux GasPressure for Q1MS
5 15 25 35 45 55
Aux GasPressure Value (psi)
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PreviousSetting=0.00
Optimum Setting=15.00
1691 %Improvement
Optimizing Capillary Temperature for Q1MS
200 250 300 350 400
Capillary Temperature (C)
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PreviousSetting=270.28
Optimum Setting=281.46
23 %Improvement
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Optimizing S-LensRF Amplitude for Q1MS
0 80 160 240 320
S-LensRFAmplitude Voltage (V)
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Apr 22,2014
PreviousSetting=83.13
NewSetting=145.19
198 %Improvement
Breakdown Curve of Ion 329.1 m/z
5 20 35 50 65 80
CollisionEnergy (V)
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Product Ions Coll.Energy
267.0 m/z 29 v
297.2 m/z 14 v
249.1 m/z 40 v
265.1 m/z 22 v
Intensity: 8.15e+04
Pressure: 1.5 mTorr
SRIGAMP DAC Scan #A: 19 Peak: 126.84; 3.62e+004
0 50 100 150 200 250 300
Parameter Setting (Arb)
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126.84
90.47
53.77
188.52
208.14
27.20
245.78
269.18
303.98
S#: 12819 FULL: PRO: 329 CT: 0.51 #A: 9 8.98e2
50 100 150 200 250 300
m/z
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205.264
73.212
267.154
229.380
248.991
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Signature: __________________________________________