Natura Biotech

Process flow chart for production of Biofertilisers & Biocontrol Agents

(Powder form)
Mother culture in test tube (purchased)
Preparation and sterilization of media in test tube (slant/seed culture/inoculum)
Transfer of inoculum from mother culture to slant
Preparation and sterilization of broth in test tube
Transfer of inoculum from slant to broth
Preparation and sterilization of broth in 100 ml conical flask
Transfer of inoculum from broth test tube to broth in 100 ml conical flask
Shaking it continuously in shaker
Preparation and sterilization of broth in 1 litre conical flask
Transfer of inoculum from broth in 100 ml conical flask to 1 litre conical flask
Shaking it continuously in shaker
Preparation and sterilization of broth in litre conical flask
Transfer of inoculum from broth in 1 litre conical flask to litre conical flask
Shaking it continuously in shaker
!uality checking
Preparation and sterilization of broth in 10 litre conical flask
1
Natura Biotech
Transfer of inoculum from broth in litre conical flask to 10 litre conical flask
Shaking it continuously in shaker
!uality checking
Sie"ing of purchased pul"erized carrier material
Sterilization of carrier material in autocla"e
#lending the broth $ith the carrier material on a metal tray
%uring the blended material by spreading in metal tray $ith polythene co"ering
!uality checking
Packing in printed lo$ density polyethylene bags
Mass packing in carton bo&es ' Storage
(ispatch / !uality checking of the stored material at the end of 1
st
) *
rd
and
th
month
+
Natura Biotech
Process flow chart for production of Biofertilisers & Biocontrol Agents
(Liquid form)
Mother culture in test tube (purchased)
Preparation and sterilization of media in test tube (slant/seed culture/inoculum)
Transfer of inoculum from mother culture to slant
Preparation and sterilization of broth in test tube
Transfer of inoculum from slant to broth
Preparation and sterilization of broth in 100 ml conical flask
Transfer of inoculum from broth in test tube to broth in 100 ml conical flask
Shaking it continuously in shaker
Preparation and sterilization of broth in 1 litre conical flask
Transfer of inoculum from broth in 100 ml conical flask to 1 litre conical flask
Shaking it continuously in shaker
Preparation and sterilization of li,uid media in litre conical flask
Transfer of inoculum from broth in 1 litre conical flask to li,uid media in litre
conical flask
Shaking it continuously in shaker
!uality checking
Preparation and sterilization of li,uid media in 10 litre conical flask
*
Natura Biotech
Transfer of inoculum from li,uid media in litre conical flask to 10 litre conical flask
Shaking it continuously in shaker
!uality checking
-ddition of preser"ati"e and stabilizing agent
-ddition of degasification agent
Packing in high density polyethylene bottles
Sticking labels on bottles
Mass packing in carton bo&es ' Storage
(ispatch / !uality checking of the stored material at the end of 1
st
) *
rd
and
th
month
.
Natura Biotech
Process flow chart for production of Vermicompost
%ollection of earth$orms
Placing the organic matter in barrels
/ntroduction of $orms into the barrels
Moisture maintenance in barrels
0ar"esting the compost once in a fortnight