PCR Guru - PCR Troubleshooting, Optimization, Tips, Tricks, Good Pra... http://www.pcrguru.

com/

This 62-page book was written with an aim of providing researchers in molecular biology, whether at
their beginner or expert stage, with a handy reference for challenging the problems associated with PCR
setup and optimization. Since it is a laboratory guide, the emphasis was done on the technical aspects of
employing PCR as a tool in molecular biology laboratories. In the Background chapter, we shortly talk
about the history of PCR and the basic science underlying it. The Procedure chapter describes what PCR
is on the bench level starting with detailed description and tips on primer design and continuing with what
are the standard protocols used to perform PCR nowadays. Various types of modifications of the standard
protocols are described the Troubleshooting chapter. The pride of this book are the unique Good
Practices and Tips chapters that are indispensible for a beginner and are something an expert would like
to have a look at. The Special Cases chapter is describing several extraordinary applications of PCR and
their optimization and troubleshooting. One of your favourite parts of this book will be the extensive
Appencices, which are listing several useful tables, figures and key protocols.

Our customers thank us for such a useful publication and ask for similar guides for other techniques such
as RNAi and Westen Blotting, which are on their way...

1. Preface
2. Chapter 1: Introduction
a. A Bit of History
b. What is PCR?
3. Chapter 2: Procedure
a. Primer Design
b. Standard PCR setup
c. Controls
d. Cycling Parameters
4. Chapter 3: Good PCR Practices
5. Chapter 4: Troubleshooting
6. Chapter 5: Tips
7. Chapter 6: Special Cases
a. Long-PCR
b. RAPD-PCR
c. Multiplex PCR
8. Appendix A: Agarose Gel Electrophoresis Protocol
9. Appendix B: Silver Staining of DNA Gels
10. Appendix C: Plasmid Miniprep Protocol
11. Appendix D: Restriction Enzyme Digestion Protocol
12. Appendix E: Protocol for DNA Concentration Estimation
by using Hoechst 33258
13. Appendix F: Formulas
14. Appendix G: Primers for Common Promoters,
Housekeeping Genes, Reporters, etc.
15. Appendix H: Tags
16. Appendix I: Thermostable DNA polymerases
17. Appendix J: Example of a Daily PCR Log
18. Appendix K: Common Buffers and Solutions
19. Useful websites
20. Figure Table of Contents
21. Glossary
22. Index
23. Acknowledgements.

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Format: PDF (Adobe Acrobat)

Delivery: e-mail

Size: 62 pages

We accept Credit Cards, PayPal and Google Checkout.

The e-Book will delivered to you via e-mail as soon as the payment is received.
You will be sent both PDF and LIT versions of the eBook.

Price: $9.99
(shipping & handling is free)

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