OBJECT: TO DETERMINE SERUM URIC ACID CONCENTRATION

FROM GIVEN SAMPLE OF BLOOD.

INTRODUCTION
Uric acid is the cata!"ic e#d $r!d%ct !& $%ri#e 'eta!"is' that are i#(ested)
s*#thesi+ed !r c!'e &r!' destr%cti!# !& tiss%e ce""s.
The %ric acid is &i"tered thr!%(h ,id#e* ("!'er%"%s a#d is &%""* reas!red thr!%(h
$r!-i'a" c!#.!"%ted t%%"es a#d is e-creted * dista" t%%"es S'a"" a'!%#t is
cata!"i+ed * %#,#!/# 'echa#is'.
It is rich i# $%ri#e c!#tai#i#( &!!ds "i,e !r(a#s 'eat )s/eet read ) heart) ,id#e* a#d
&"esh 'eat !& $!%"tr* a#d &ish a#d s'a"" a'!%#t i# e((s a#d "itt"e !r #! a'!%#t i#
'i",.
MET0OD OF DETERMINATION
E#+*'atic ca"!ri'etric 'eth!d 1%ricase 'eth!d2
PRINCIPLE
The deter'i#ati!# !& %ric acid * reacti!# /ith %ricase. The &!r'ed 0
3
O
3r
react %#der cata"*sis !& $er!-idase /ith 4)5 6 di7ch"!r! 737h*dr!-*
e#+e#s%"&!#ic acid 1Dc 0BS2 a#d 87 a'i#! $he#a+!#e 1PAP2 t! (i.e a red
6.i!"e#t 9%i#!#e'i#e d*e as i#dicat!r a#d that ca# e 'eas%red
$h!t!'eterca""*.
COLLECTION OF SAMPLE
Ta,e 3 CC !& "!!d sa'$"e i# c"ea# test t%e a#d ce#tri&%(e it &!r &e/
'i#%tes t! (et the ser%' 1Ser%' he$ari#i+ed !r Ser%' :ith EDTA
1 a#tic!a(%"a#t 2 is re9%ired.
EQUIPMENTS:
Photometry, centrifuge machine, water bath, pipettes,
Cuette, test Tubes, syringes!
SAMPLE:
Ser%' is re9%ired.
REAGENTS:
E#+*'atic reacti!# Rea(e#t C!#tai#s 8 6 a'i#! $he#a+!#e) $er!-idase
Uricase a#d B%&&er 1;.<<2
Sta#dard S!"%ti!# that c!#tai#s Uric acid = '(>d"
PROCEDURE
:a.e"e#(th 53< #'
O$tica" $ath ?c'
Te'$erat%re 4;
<
C
Meas%re'e#t: A(ai#st rea(e#t B"a#, test t%e

S!"%ti!# Sa'$"e Sta#dard "a#,
Seri%' <.<8'" 7 7
Sta#dard 7 <.<8 '" 7
Rea(e#t ? '" ? '" ?'"
Mi-) i#c%ates &!r ?< 'i#%tes at 3< t! 35
<
C

!r 5 'i#%tes at 4;
<
C
Meas%re the as!ra#ce !& the sa'$"e > sta#dard a(ai#st the rea(e#t "a#, /ithi# ?5
'i#%tes
Ca"c%"ati!#
C@ = -


A sa'$"e
'( >d"
A Sta#dard
C@ 8;A -


A sa'$"e
B'!" >d"
A Sta#dard
VALUES
Ma# "!!d 4.8 7;.< '(>d"
Fe'a"e "!!d 3.8 75.; '(>d"
Uri#e 35< 7;5<'(>38
th

CLINICAL IMPORTANCE
?. I#creased %ric acid a!.e ; '( $er ?<< '" is ca""ed h*$er%ricae'ia
3. %ric acid 'a* e i#&"%e#ced * #%'er !& &act!rs
Race: 'ar,ed"* raised i# Ma!ris
Se-
0i(her i# 'a"es 1<.=> ?.A '(>d"2 d%e t! i#crease re#a" c"ear#ess !&
%ric acid i# &e'a"es a#d i#crease !d* 'ass !& 'a"es
B!d* 'ass: i#creased i# !esit*) $r!a"* d%e t! dietr* haits
i#creased $%ri#e t%r#!.er a#d h*$ertri("*ceridae#ia
S!ci!ec!#!'ic: '!re i# hi(her S!ci!ec!#!'ic (r!%$ tha# the "!/er !#es.
DIET:
?. E"e.ated "e.e"s /ith hi(h 'eat i#ta,e
3. A"c!h!" i#creases $"as'a %ric acid "e.e" *
1a2 i#hiiti!# !& %ric acid e-creti!# * "actic acid 1c!'$etiti.e2
12 i#creased t%r#!.er !& ade#i#e.
1c2 I#creased $%ri#e i#ta,e d%e t! ear *east
4. Fasti#( i#crease ,et!#es "e.e") the C h*dr! %t*ric acid c!'$etiti.e"*
i#hiits %ric acid rete#ti!# * re#a" t%%"es.
8. Decreased &"%id i#ta,e "eads t! i#creased rete#ti!# !& %ric acid * the
,id#e*.
EDCERCISE:
I#creased "actic acid "e.e" i# the "!!d i#creases the $"as'a %rate.
Pre(#a#c*:
Le.e"s 'a* i#itia""* decrease %$ t! 35 E 1&irst tri'ester2) %t rise %$ t! 3<
E hi(her at ter' as c!'$ared t! the #!# $re(#a#t &e'a"es. This e"e.ati!#
'a* $ersist &!r a '!#th !r t/!.
0FPERURICAEMIA:
1I#creased %ric acid "e.e" i# the "!!d a!.e ; '( > ?<< '"2 /hich ca# "ead
t! (!%t is $r!d%ced * a #%'er !& ca%ses. I# '!st !& the ca%ses the
i!che'ica" de&ect ca# #!t e e-$"ai#ed. 0!/e.er) the ,#!/# i!che'ica"
de&ect are as &!""!/s.
?. Reacti!# "eadi#( t! !.er $r!d%cti!# !& $h!s$h!ri!s*" $*r!$h!s$hate.
3. Reacti!# "eadi#( t! !.er$r!d%cti!# !& ("%ta'i#e.
4. De&icie#c* !& h*$!-a#thi#e> (%a#i#e> $h!s$h!ri!s*" tra#s&erase10GPRT2.
8. I#creased $r!d%cti!# !& !r(a#ic acids ca# a"s! "ead t! e"e.ated "e.e"s
!& %ric acid.
0*$er%ricae'ia %ric acid "e.e" a!.e ; '( > ?<<'" "eads t! (!%t) /hich is
!& t/! t*$es.
PRIMARF GOUT
Pri'ar* (!%t is a (e#etic dis!rder !& $%ri#e 'eta!"is'. Its i#cide#ce is
'!re i# 'a"es tha# &e'a"e.
SECONDARF GOUT
Sec!#dar* (!%t is ac9%ired de&ect. E-a'$"es are Chr!#ic Ne$hritis)
Le%,e'ia a#d $!"*c*the'ia