VI TEKMS Simplified User Guide Version 4 page 1

VI TEKMS Simplified User Guide Version 4 page 2

Table of contents
VITEK MS instrument
Configuration...
Workflow
Material and consumables ..
Protocol ID&AST..
Protocol ID
Protocol AST. page 10
page 4
page 5
page 6
page 7
page 8
page 9
VI TEKMS Simplified User Guide Version 4 page 3
VITEK MS Prep Station page 11
MYLA Slides composition.... page 12
VITEK MS Acquisition Station. pages 13-15
MYLA : Results to be reviewed... pages 16-19
MYLA : Results to be approved.. pages 20-21
MYLA : Electronic Signature page 22
MYLA : AST review..... page 23
VITEK MS Users maintenance... page 24
Table of contents
VI TEKMS Simplified User Guide Version 4 page 4
VITEK MS Instrument
Indicates an alarm
Status OK
VI TEKMS Simplified User Guide Version 4 page 5
MS-ID Database
( CE/IVD) embedded into Myla
TM
P ow er Io n is i ng Vac uu m Negat iv e P ow er Io n is i ng Vac uu m Negat iv e P ow er Io n is i ng Vac uu m Negat iv e
P ow er Io n is i ng Vac uu m Negat iv e P ow er Io n is i ng Vac uu m Negat iv e P ow er Io n is i ng Vac uu m Negat iv e
Prep Station
Configuration
VI TEKMS Simplified User Guide Version 4 page 6
Workflow
Acquisition Station
Po w er I o n i s i n g Vac u u m Neg a t i v e Po w er I o n i s i n g Vac u u m Neg a t i v e Po w er I o n i s i n g Vac u u m Neg a t i v e
Prep Station
Slide
description
Spectra
Slide
description
Peak lists
Slide
description
MS-ID Database
( CE/IVD) embedded into MylaTM
Step 1
Operator
Information
Step 3
Step 2
VI TEKMS Simplified User Guide Version 4 page 7
Material & consumables
For Bacteria & Fungi
Pipette ( 0.1-2.5l) +tips without filter
Loops 1l
VITEK MS-DS Target Slides 48 spots ref 410893
Calibrant : LyfoCults Plus E.coli ATCC8739Ref. 301145
Columbia agar + 5 % Sheep blood Ref. 43 041 / 43 049
or Trypcase Soy agar + 5 % Sheep blood Ref. 43 001 / 43 009
Matrix: VITEK MS-CHCA ref 411071
For Molds & Mycoplasma
Please refer to :
For Yeast
VITEK MS-FA ref 411072
VI TEKMS Simplified User Guide Version 4 page 8
Protocol
Identification VITEK MS
& AST VITEK 2
Prep Station
Matrix deposit
1L CHCA *
Calibrant
Deposit
+ Matrix
Step 2
Step 4
A
B
Step3
McF
* Prior extraction for yeasts only: 0,5L Formic acid deposit
Step 1
VI TEKMS Simplified User Guide Version 4 page 9
Protocol
Identification VITEK MS only
Prep Station
Matrix deposit
1L CHCA *
Calibrant
Deposit
+ Matrix
Step 2
Step 3
* Prior extraction for yeasts only: 0,5L Formic acid deposit
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Step 1
VI TEKMS Simplified User Guide Version 4 page 10
Protocol
AST VITEK 2 only
Prep Station
McF
* Prior extraction for yeasts only: 0,5L Formic acid deposit
VI TEKMS Simplified User Guide Version 4 page 11
6 .Scan the
VITEK2 Card
barcode and
place the card
on the cassette
1 . Scan the
target slide
2 . Scan the Lab
ID on the plate
3 . Choose
between
Bacteria or Fungi
4 . Deposit the
spots
5 . Prepare the
bacterial McF
suspension
7 . F4 Validate
Prep Station
VI TEKMS Simplified User Guide Version 4 page 12
Slides Composition
Homepage
VI TEKMS Simplified User Guide Version 4 page 13
Acquisition Station
1. Open the door
2. Scan the
target slides
barecodes
3. VITEK MS
retrieves target
slides
information
4. Load the
target slides into
the VITEK MS
5. Start the
Acquisition
6. Acquisition in
progress
7. End of
acquisition
VI TEKMS Simplified User Guide Version 4 page 14
Acquisition Station:
Spots color code
VI TEKMS Simplified User Guide Version 4 page 15
Acquisition Station:
Spectra features
Average of all the spectra
collected for the sample
Averaged data after
processing
(smoothing, threshold
substraction, peak
detection)
Peak list sent to the MS-ID
server
VI TEKMS Simplified User Guide Version 4 page 16
Results to be reviewed
Detail of VITEKMS ID to be
reviewed
Homepage
Technologist Dashboard
VI TEKMS Simplified User Guide Version 4 page 17
Refresh the list of results
Review selected identification results.
The results are removed from this list
and will be displayed in the Reviewed tab
Review all identification results with
a high confidence level.
The results are removed from this list
and will be displayed in the Reviewed tab
Discard selected identification results.
The results are removed from this list
and will be displayed in the Reviewed tab
Enter and assign a comment for the
selected result(s) if applicable.
Print a report file of the selected
result(s) to be reviewed
Results to be reviewed
Indicates that an organism is a highly pathogenic
organism or a critical pathogen
This result must be review using the following icon
VI TEKMS Simplified User Guide Version 4 page 18
Results to be reviewed
Rules for Consolidation :
-The consolidated identification result can be sent as it is.
- Consolidate the identification by selecting a result
- Result cannot be consolidated, repeat the analysis
(1) Select the
organism name
(2) Validate the selected
organism name
Optional: Display a pop-up
window to enter comments
for the selected result(s)
Indicates that an organism has been selected
VI TEKMS Simplified User Guide Version 4 page 19
Results to be reviewed
VI TEKMS Simplified User Guide Version 4 page 20
Results to be approved
Only if the 'Review and Approve for result
Validation' setting is enabled in MYLA
Detail of VITEKMS ID to be
approved
Homepage
Manager Dashboard
VI TEKMS Simplified User Guide Version 4 page 21
Refresh the list of results
Approve the selected result(s)
The results are removed from this list and
will be displayed in the Approved tab
Approve all identification results with
a high confidence level
The results are removed from this list
and will be displayed in the Approved tab
Reject the selected result(s)
The rejected results are removed from this
list and will be displayed in the To be
reviewedtab with the status To rework
Display a pop-up window to enter
comments for the selected result(s)
Print a report file of the selected
result(s) to be approved
Results to be approved
Only if the 'Review and Approve for result
Validation' setting is enabled in MYLA
When a comment is entered, the button
is displayed on the result line
VI TEKMS Simplified User Guide Version 4 page 22
Electronic Signature
Only if the Electronic Signature
option setting is enabled in MYLA
22
When you review, discard, approve & reject result(s), an Electronic Signature
has to be entered to confirm the sending of results.
The 1st time, you have to enter the User name and Password, then only the
Password is required.
VI TEKMS Simplified User Guide Version 4 page 23
AST Review
VI TEKMS Simplified User Guide Version 4 page 24
VITEK

MS Users maintenance
Maintenance Operation Frequency Refer to
Cleaning the VITEK MS
and the Acquisition Station
+ Acquisition Station monitor
To be established according your
internal procedure .
To be written in your SOP
Chapter 5 Maintenance
Cleaning the Adapter To be established according your
internal procedure .
To be written in your SOP
Changing the desiccant When the desiccant turns to pink
Cleaning of the O-ring seal & the
sealing face of the door To be established according your
internal procedure .
To be written in your SOP
Chapter 12