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INTRODUCTION
DEVELOPMENT
PHYSICAL PROPERTIES
CHEMICAL PROPERTIES
TYPES OF DENTIN
HISTOLOGY OF DENTIN
CLINICAL CONSIDERATIONS
REFERENCES
CONCLUSION
Submitted by:
Dr. Anshuman Khaitan
Dept. of Cons. and Endo.
College of Dental Sciences
Davanagere
INTRODUCTION
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Dentin provides the bulk and general form of the tooth and is characterized as a
hard tissue with tubules throughout its thickness. It forms slightly before the
enamel; it determines the shape of the crown, including the cusps and ridges and
the number and size of the roots. Along the crown, the dentin is covered by
enamel, along the root by cementum. It encloses the dental pulp, with which it
shares a common origin from the dental papilla. The dentin and pulp can be
considered as a single developmental and functional unit, often described as
pulpo dentinal complex.
DEVELOPMENT
Dentinogenesis : It is the process of dentin formation. The dentin formation
begins in late-bell stage. Dentine is formed by the odontoblast cells that
differentiate from ecto-mesenchymal cells of dental papilla. Thus dental papilla
is a formative organ of the dentin. The dental pulp also develops from dental
papilla which is mesodermal in origin.
Dentinogenesis occurs in the following 3 stages:
A)
Cyto-differentiation.
B)
Matrix formation.
C)
Mineralization.
increases. The nucleus moves away from basement membrane. It reverses the
polarity of cells. These cells are called true odontoblasts which secrete predentin.
Once the formation of predentin at cuspal/ incisal region begins the
differentiation of new odontoblasts takes place further apically in dental papilla.
Initially daily increments of approximately 4 m/day of dentin formed. Once
crown formation completes the dentin formation slows down to 1m / day. The
root dentine formation resembles the cyto-differentiation of crown but it requires
proliferation of epithelial sheath (Hertwigs epithelial root sheath).
Matrix formation : The differentiated odontoblasts will have all features of the
secretory cell i.e. an abudance of rough endo-plasmic reticulum, a welldeveloped golgi apparatus, mitochondria and secretory granules. The procollagen
synthesized in the rough endoplasm reticulum is transferred to golgi-apparatus,
and finally appear in secretory granules. The matrix mainly consists of collagen
(Type I), proteoglycans and glycoproteins. As each increment of pre-dentine
formed it remains for a day before calcified layer of predentine is formed. The
initial dentin deposition along the cusp tip is called VonKorffs fiber. These are
argyrophilic fiber (Stains black with silver). As the matrix formation continues,
the odontoblast leaves an extension called the odontoblastic process.
Mineralization : The mineralization occurs in a globular pattern. The earliest
crystal deposition is in the form of very fine plates of hydroxyapatite on the
surface of collagen fibrils and in the ground substances. Subsequently HA
crystals are laid down within the collagen fibrils. These crystals are arranged
with their long aixs is parallel to the fibril axis. This crystal deposition appears
to takes place from a common center in a spherulite form.
The peritubular region becomes highly mineralized at a very early stage. The
final crystal size remains very small about 3nm in thickness and 100nm in
length. The HA crystals of dentin resembles that of cementum and bone and are
300 times smaller than the enamel crystals.
STRUCTURE OF DENTIN
The dentinal matrix of collagen fibers is arranged in a random network. As
dentin calcifies, the hydroxyapatite crystals mask the individual collagen fibers.
The bodies of the odontoblasts are arranged in a layer on the pulpal surface of
the dentin and only their cytoplasmic processes are included in the tubules in the
mineralized matrix.
predentin and calcified dentin within one tubule and terminates in a branching
network at the junction with enamel or cementum. Tubules are found throughout
normal dentin and are therefore characteristic of it.
PHYSICAL PROPERTIES
It is light yellow in colour and becomes darker with age and less
translucent It is harder than bone and cementum but softer and less brittle
than enamel.
Dentine has greater compressive strength and tensile strength than enamel
because it is traversed by tubules.
The lower mineral salt content in dentin renders it more radiolucent than
enamel.
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MATERIAL
DENSITY
THERMAL
COEFFICIENT OF THERMAL
G/CM3
CONDUCTIVITY
EXPANSION
W/MK
ENAMEL
DENTIN
2.14
0.57
0.75
GIC
2.19
0.51-0.72
0.96
ENAMEL
2.47
0.93
COMPOSITE
1.6-2.4
1.09-1.37
1.2-4.4
RELATIVE
CHEMICAL PROPERTIES
70% - Inorganic material
20% - Organic Materials
10% - Water
TO
Intensity of stimulus.
b.
Duration of stimulus.
c.
Vitality of pulp.
The tertiary dentin may or may not have a regularly arranged of tubules or no
tubules at all. Sometimes the odontoblasts are trapped in the dentin and they are
called osteodentin.
It differs from the primary dentin by having both type I, Type II collagen
fibers. Usually there is no continuity between tertiary and 1 and 2 dentin
tubules. This helps in minimizing the dentin permeability and protecting
underlying dental pulp.
HISTOLOGY OF DENTIN
Dentinal tubules:
The dentinal matrix contains tubules, each or which ranges from about 1 to 2m
in diameter at its outer end and 3 to 4m at its pulpal side. The number of
tubules is about 15,000 /mm 2 near the dentin enamel junction and it is
65,000mm 2 near the pulpal surface.
The ratio between the outer and inner surfaces of dentin is about 5:1.
Accordingly tubules are farther apart in periphery and more closely packed near
the pulp (3-4 m) and smaller at their outer ends (1 m).
The ratio between the number of tubules per unit area on pulpal and outer
surface of dentin is about 4:1.
The dentinal tubules are fine canals that extend across the entire width of the
dentin. They contain odontoblastic process. The course of the dentinal tubules
follows a gentle curve, which is S Shaped. They show two curvatures primary
curvature and secondary converters.
Primary curvature start at right angle from the pulpal surface, the convexity of
this curved course is directed towards the apex of the root and the curvature in
the outer half is directed towards the occlusal or incisal surface.
These tubules end perpendicular to the D.E. Junction and D-C junction. It is
almost straight at the root apex, incisal edges and cusps. Over their entire,
length, the tubules exhibit minute relatively regular secondary curvatures.
Canaliculi or microtubules:
The dentinal tubules have lateral branches throughout the dentin termed as
canaliculi. These canaliculi are1m or less in diameter and originate more or less at
right angle to the main tubule.
Enamel spindles:
Near the DEJ, the dentinal tubule divides into several terminal branches and
forms an intercommunicating and anatomizing network. Some dentinal tubules
extend into the enamel for several millimeters. These are termed as enamel
spindles.
Peritubular dentin:
The dentin that immediately surrounds the dentinal tubules is called peritubular
dentin. This dentin forms the walls of the tubules. It is more highly mineralized
than the intertubular dentin. It is completely broken down and disappears on
being subjected to routine decalcification methods.
Intertubular dentin:
The main body of the dentin is composed of intertubular dentin. It is located
between the dentinal tubules or between the zones of peritubular dentin.
Although it is highly mineralized, this matrix, like bone and cementum is
retained after decalcification. About one half of its volume is organic matrix,
especially collagen fibres that are randomly oriented around the dentinal tubules.
The fibres have a lattice like arrangement coursing in gentle curves between the
tubules and their peri-tubular zones. Hydroxyapatite crystals are formed along
the fibres. Provides tensile strength to dentin.
Inter globular dentin:
Sometimes mineralization of dentin begins in small globular areas that fail to
fuse into a homogenous mass. This results in zone of hypo-mineralization
between the globules. These zones are known as interglobular dentin. Inter
globular dentin forms in the crown of teeth in the circumpulpal dentin just below
the mantle dentin, and it follows the incremental pattern. The dentinal tubules
pass uninterruptedly through interglobular dentin, thus demonstrating defects of
mineralization and not of matrix formation.
Pre dentin:
Predentin is located adjacent to the pulpal tissue and is 2m to 6m wide. It is
the first formed dentin and is not mineralized. As the collagen fibres undergo
mineralization at the pre-dentin front, the predentin then becomes dentin and a
new layer of predentin forms circumpulpally. NOTE:-loss of predentin is one of
the factors for internal resorption.
Granular layer:
When dry ground section of the root dentin is visualized in transmitted light,
there is a zone adjacent to the cementum that appears granular. This is known as
tomes granular layer or hyaline layer of Hopewell smith. This zone increases
slightly in amount from the Cementoenamel junction to the root apex and is
believed to be caused by a coalescing and looping of the terminal portions of the
dentinal tubules.
The cause of development of this zone is probably similar to the branching and
beveling of the tubules at the dentinoenamel junctions. It Serves to bond
cementum to dentin.
Incremental Lines:
The incremental lines von ebner or imbrigation lines or Stria of Retzius appear
as fine lines (or) striations in dentin. They run at right angles to the dentinal
tubules and correspond to the incremental lines in enamel (or) bone.
These lines reflect the daily rhythmic, recurrent deposition of dentin matrix as
well as interruption in the daily formative process. The distance between lines
varies from 4 to 8 m in the crown to much less in the root. The course of the
lines indicates the growth pattern of the dentin.
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INNERVATIONS OF DENTIN
Intertubular nerves:
Dentinal tubules contain numerous nerve endings in the predentin and inner
dentin no further than 100 to 150 m from the pulp. Most of these small
vesiculated endings are located in the tubules in the coronal zone, specifically in
the pulp horns. The nerves and their terminals are found in close association with
the odontoblast process within the tubule. It is believed that most of these are
terminal processes of the myelinated nerve fibres of the dental pulp.
DEVELOPMENTAL DISTURBANCES OF DENTIN:
Dentinogenesis imperfecta
Dentin dysplasia
Regional odontodysplasia
the abnormal DEJ, where normal scalloping is absent. The dentin undergoes
rapid attrition (occlusal, incisal surface). These teeth are less suitable to caries
than the normal teeth.
Radiographically there is a partial obliteration of pulp chambers. There is a
continued formation of dentin in root canals.
In type III the important radiographic feature is shell teeth. Here the enamel
appears normal and dentin is very thin with enormous pulp chambers. This large
pulp chamber is due to defective dentin and enamel formation.
Dentin-dysplasia (root less teeth)
It is rare dental anomaly characterized by normal enamel, abnormal dentin with
atypical pulp morphology.
Witkop classified it into following types:
Type I Radicular dentin dysplasia.
Type II Coronal dentin dysplasia.
Type I is more common.
Both are hereditary in origin and A.D. type.
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Type I Radicular: The roots are short and blunt. In deciduous dentition there is a
complete or partial obliteration of pulp chamber. In permanent teeth crescent
shaped pulp chamber is seen.
Type II Coronal: The pulp chambers are obliterated in deciduous tooth as in type
I. The permanent teeth show abnormal large pulp chamber in coronal portion
described as thistle tube appearance. Small pulp stones are also seen in the
chamber.
Histological features:
It shows tubular dentin, osteodentin and fused denticles. The pulp chamber is
obliterated. Normal dentin formation is blocked so the dentin formation occur
around the obstacles giving characteristic appearance called lava flowing
around boulders.
Regional odontodysplasia or Ghost teeth
The maxillary anteriors are more commonly involved here. It affects both
dentition. The etiology of disease is unknown but it is thought that it could be
due to a latent virus in odontogenic epithelium, which become active in
subsequent period.
The teeth affected shows delay or failure of eruption. The shape of teeth is
altered, with irregular mineralization.
Radiographically teeth show marked reduction in radiodensity gives Ghost teeth
appearance. The enamel and dentin is very thin and pulp chamber is usually
large. Histologically characterized by a marked reduction in the amount of
dentin, widening of predentin layer and large areas of interglobular dentin with
irregular tubular pattern. Due to poor cosmetic appearance extraction of teeth
and restoration with prosthetic appliances is usually indicated.
AGE AND FUNCTIONAL CHANGES
i.
Vitality of dentin.
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ii.
Reparative dentin.
iii.
Dead tracts.
iv.
Vitality of dentin:
Since the odontoblast and its process are an integral part of the dentin, there is
no doubt that dentin is vital tissue. If vitality is understood to be the capacity of
the tissue to react to physiologic and pathologic stimuli, dentin must be
considered a vital tissue.
Dentin is laid down throughout life, although after the teeth have erupted and
have been functioning for a short time, dentinogeneis slows and further dentin
formation is at a much slower rate.
Reparative dentin:
If by extensive abrasion, erosion, caries or operative procedures, the odontoblast
process are exposed or cut, the odontoblasts die or if they live, deposit reparative
dentin. The odontoblasts that are killed are replaced by the migration of
undifferentiated cells arising in deeper regions of the pulp.
Reparative dentin is characterized as having fewer and more twisted tubules than
dentin.
Dead tracts:
In dried ground sections of normal dentin the odontoblast processes disintegrate
and the empty tubules are filled with air. They appear black in transmitted light
and white in reflected light. They extend from the DEJ to the corresponding area
of dentin pulp interface. In most instances, the dead tracts are sealed at their
pulpal aspect by the formation of reparative dentin. These areas demonstrate
decreased sensitivity and appear to a greater extent in older teeth.
Sclerotic / transparent dentin:
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and
dissolves;
3) The loss of structural integrity is followed by invasion of bacteria.
The five different zones are most clearly distinguished in slowly advancing
lesions:
1) Zone 1: Normal dentin
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Permeability of occlusal dentin is higher over the pulp horns than at the center of the
occlusal surface, proximal dentin is more permeable than occlusal dentin, and the
coronal dentin is more permeable than root dentin.
Protective function of dentin
A key function of enamel and dentin is thermal insulation of pulp. Most
restorative materials are not as insulating as dentin and thermal insults may
occur during intraoral temperature changes. The need for thermal insulation is
greatest for metallic restorations. Thermal insulation is proportional to the
thickness of insulating material. Approximately 2mm of dentin or an equivalent
thickness of material should exist to protect the pulp.
Bacterial toxins, strong drugs, undue operative trauma, unnecessary thermal
changes or irritating restorative materials should not insult the cells of the
exposed dentin. It should be remembered that when 1 mm 2 of dentin is exposed,
about 30,000 living cells are damaged. It is advisable to seal the exposed dentin
surface with a non-irritating insulating substance.
LINERS: liners are thin layers of material used primarily to provide a barrier to protect the dentin
from residual reactants diffusing out of a restoration and or oral fluids, which may penetrate
leaky tooth restoration interfaces. they also contribute initial electric insulation and generate
some thermal protection. the need for liners is greatest with metallic restorations that are not well
bonded to tooth structure and which are not insulating, such as amalgam and cast gold.
BASES : they replace the missing dentin and provide thermal insulation to the
pulp. They are placed in a thickness of 0.5 to 2 mm and they have sufficient
strength to support the overlying restoration.
Pulp capping
Pulp capping is the procedure by which a layer of calcium hydroxide is placed
over a thin layer dentin remaining over the pulp.
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TRANSDUCTION THEORY
HYDRODYNAMIC THRORY
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It was proposed by Scott Stella. It states that stimuli in some manner (which is
unknown) reach the nerve endings of inner dentin. There is little scientific
support for this theory.
Hydrodynamic Theory
Most popular theory proposed by Gysi, supported by Branstorm.
Various stimuli such as heart, cold, air, blast dessication or mechanical pressure
affect fluid movement in the dentinal tubules. The fluid movement, either inward
or outward stimulates pain mechanism in the tubules by mechanical disturbance
of the nerves closely associated with odontoblast and its processes. Thus, these
endings may act as mechanoreceptors as they are affected by mechanical
displacement of tubular fluid.
Transduction theory
According to this theory, the odontoblast is the primary structure excited by the
stimulus and that the impulse is transmitted to the nerve endings in the inner
dentin. This theory is not popular since there are no neurotransmitter vesicles in
the odotoblast process to facilitate the synapse.
Treatment
The treatment options are
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Use of topical agents containing potassium nitrate, silver nitrate, CPP ACP,
fluorides, oxalates etc.
Placement of restorative material such as glass ionomer cement.
Dentin bonding
While bonding to enamel is predictable, bonding to dentin is not so easy. Dentin
bonding is difficult due to the following reasons:
Dentin is a dynamic tissue that shows changes due to aging, caries or
restorative procedures.
It has considerable amount of organic material and water when compared to
enamel
Dentinal tubules are filled with dentinal fluid.
Cut dentin surface is covered by a smear layer that blocks the dentinal tubules
and reduces its permeability.
Dentin is in close proximity to the pulp, so different chemicals used for
etching and bonding may irritate the pulp.
Role of smear layer in dentin bonding:
The smear layer is an amorphous, relatively smooth layer of microcrystalline
debris, which appears on all cut surface of the dentin regardless of the cutting
procedure used.
It has 2 components:
1.
2.
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It cannot be seen with the naked eye. Whether or not the smear layer should be
left in place is a matter of controversy. One view is that the smear layer should
be left in place because it effectively seals the dentinal tubules. Removal of the
smear layer by acid treatment would open and widen the tubules about three fold
at the surface. This would greatly increase dentin permeability. The other view is
that the smear layer contains microorganism, which would multiply and produce
infection if left alone. Moreover the smear layer would prevent proper bonding
of restorative material to the dentinal wall by serving as a barrier to the
penetration of resin to the underlying dentin substrate.
Recent generations of dentin adhesives involves modification of the smear layer
to improve the bond strength to dentin.
Conditioning of dentin:
Conditioning of dentin is defined as any alteration of dentin done after the
creation of dentin cutting debris, termed the smear layer.
The objective of dentin conditioning is to create a surface capable of micro
-mechanical and possibly chemical bonding to a dentin-bonding agent.
Conditioning of dentin may be done by several means :
Chemicals
-
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Etching removes the smear layer and exposes the collagen fibrils.
Primers penetrates the collagen network.
Adhesive resins along with the primers form resin microtags within the
intertubular dentin and surround the collagen fibers upon curing.
The hybrid layer is also called as the resin reinforced layer or the resin dentin
interdiffusion zone
Moist vs dry dentin surfaces :
Vital dentin is inherently wet; therefore, complete drying of dentin is difficult to
achieve clinically. The use of adhesive systems on moist dentin is made possible
by incorporation of the organic solvents acetone or ethanol in the primers or
adhesives. Because the solvent can displace water from both the dentin surface
and the moist collagen network, it promotes the infiltration of resin monomers
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throughout the collagen network. The "wet bonding" technique has been shown
repeatedly to enhance bond strengths because water preserves the porosity of
collagen network available for monomer interdiffusion.
dried with air, the collagen undergoes immediate collapse and prevents resin
monomers from penetrating it. Thus excess water after acid etching and rinsing
should be removed with a damp cotton pellet, bush or a tissue paper and air
drying should be avoided.
REFERENCES
Orbans: Oral Histology and Embrology
Oral Anatomy Histology and Embryology: Berkovitz
Oral Histology: James K Avery
Oral Histology: Tencate
Dental Pulp: Seltzer and Bender
Operative Dentistry: Sturdevant
Operative dentistry - Modern theory and practice: Marzouk
Text book of operative dentistry - Vimal Sikri
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CONCLUSION
The complexity of dentinal structures makes restorative and rehabilitative
procedures highly challenging. Thus it is imperative that every clinician should
assimilate and apply the knowledge of dentinal histology and morphology to
obtain successful clinical result.
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