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(Introduction to Microscopy)
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Definition of Microscope
An instrument used in magnifying objects too small to be seen with the naked eye.
Different Types of Microscope
Generally, microscopes are subdivided into two categories, light microscopes which utilize transmitted light for
detection of small objects, and electron microscopes which uses electron beams reflected on the electrostatic and
electromagnetic lenses to illuminate and magnify objects up to 2 million times.
Several types of microscope have been invented as a result of the different requirements of various scientific
fields (see table below for different microscopes and their distinctive feature).
Microscope
Simple Microscope
Compound Microscope
Dissecting Microscope
Fluorescence Microscope
Phase Contrast
Microscope
Microscope
Scanning Electron
Microscope
Transmission Electron
Microscope
Light Microscope
Distinctive Feature/s
-utilizes a single lens to magnify
-has a system of lenses for magnifying objects; different stains are utilized to provide
image contrast
-gives a three-dimensional image of the specimen
-reveals transparent specimens when these are treated with special dyes and
illuminated with ultraviolet
-converts normally invisible differences in the optical path of light into visible lightdark differences
Electron Microscope
Distinctive Feature
-used to produce detailed pictures of specimens surface features
-gives similar image as in a compound microscope, but can reach magnifications of
up to 750,000 times
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Compound Microscope
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