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-AFIQ FAHIMY-
2nd year
Section 3
-Staph.Epidermitis
Staph.Aureus
-Staph.saprophyticus
NUTRIENT AGAR :
BLOOD AGAR :
-β-haemolysis
(iv)Clumping factor
(v)Capsular polysaccharide
(vi)Surface receptor
TOXINS ENZYMES
-septic metastasis
• Enterotoxin(A-F) :
-superantigens
• Liquefies Gelatin
Staph.Epidemidis Staph.Saprophyticus
DIFFERENTIATION
Sensitive Resistant
(Novo-Biocin test)
Disease : Disease :
Important group
Differentiation
GROUP A GROUP B
(Strept.Pyogenes) (Strept.Agalactiae)
Bacitracin test
α- haemolytic:
-green pigmentation
-Strept.Viridans
-Strept pneumoniae
Haemolytic
Properties
β- haemolytic:
-Strept.Pyogenes
Non-heamolytic:
-No haemolysis
-green pigmentation
-Strept.Viridans
-Strept.pneumoniae
β- haemolytic:
-Strept.Pyogenes
Streptococci in chain
-facultative anaerobes
Clinical findings:
Biochemical reaction :
- catalase –ve
• Haemolysins:
(a)Streptolysin O (SLO)
-oxygen labile
(b)Streptolysin S(SLS)
-not antigenic
-Oxygen stable
(ASO)
STREP.VIRIDANS
STREP.PNEUMONIAE
VIRULENCE FACTOR :
TYPING OF STREP.PNEUMONIAE
STREPTOCOCCI PNEUMONIAE
Neisseria smear by
methylene blue stain
-aerobe organism
Culture:
Biochemical reactions :
-Oxidase +ve
Determinants of pathogeneticity :
(i)capsular polysaccharide
(iii)Pili
(iv)Ig A proteases
Clinical Findings:
Treatment :
Penicilin G
to differentiate it with
diplococci pneumoniae
which are spherical..
Morphology :
Biochemical reactions :
-Selective Media : Mcleod’s Tullerite blood agar (appear grey or black colonies)
-Blood agar
• powerful exotoxin
• Only lysogenised strain are toxigenic & virulent
• heat labile
• Highly toxic
• Highly Anitgenic
• Separate into 2 fragments :
Fragment A : inhibit polypeptide chain elongation in the presence of NAD by inactivating the
Toxigenicity(virulence) tests
• Elek’s test
• PCR
• Enzyme-linked immunosorbent assays (ELISA)
• Historical in-vivo test
Treatment :
Prophylaxis :
• Fluid toxoid
• Aluminium precipitated toxoid (common combined with tetanus toxoid(T) and/or with pertussis
Vacccine(P))
Fluid Media
Nutrient Broth
Uses :
Test motility of bacteria
(in tube)
• Deep Agar
Constituents : Broth + 2-3% Agar
Uses :
to differentiate bacteria’s growth according
oxygen requirement
Uses:
1)Keeping stock for cultures for stab
inoculation
SOLID MEDIA
NAME OF MEDIA CHARACTERISTICS
Blood Agar
Uses :
Uses :
Uses :
demonstrate morphology of
C.Diphteria
Uses :
Sugar : Lactose
(fermented by prevalent intestinal
organism,e.g: E-coli)
Uses :
To grow Gram –ve bacilli
Indicator : Bromothymol-blue
pH : 8.0 -8.6
Uses :
Selective isolation of Vibrio
Cholerae
Sugar : Mannitol
Uses :
LJ + Glycerol :
Enhance growth of human type
(M.Tuberculosis)
LJ + pyruvate :
Enhance growth of bovine type
(M.Bovis)
Uses :
cultivation of tubercle Bacilli
A liquid media
Uses :
Favour growth of
Thypoid & Parathypoid
Enterococci
Culture :
Selective test :
Clinical Findings:
Urinary tract infections, endocariditis , biliary tract infections , ear infections, suppurative abdominal
lesions
Novobiocin Test
Optochin Test
Bacitracin Test
àepidemiological study
Widal Rack
(haemolysis)
+ve Serum
(no-haemolysis)