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sulfur. This thiophosphate acts the same as a normal phosphate group, although there is a slight bias
against it by most polymerases. The maximum theoretical specic activity is 1,494 Ci/mmol (55.28
PBq/mol).
Examples of radionuclei
Tritium (hydrogen-3) is a very low energy emitter
that can be used to label proteins, nucleic acids,
drugs and toxins, but requires a tritium-specic lm
or a tritium-specic phosphor screen. In a liquid
scintillation assay (LSA), the eciency is 2050%,
depending on the scintillation cocktail used . The
maximum theoretical specic activity of tritium is
28.8 Ci/mmol (1.066 PBq/mol). However, there
is often more than one tritium atom per molecule:
for example, tritiated UTP is sold by most suppliers with carbons 5 and 6 each bonded to a tritium
atom. C-14, S-35 and P-33 have similar emission
energies. P-32 and I-125 are higher energy emitters
-> inaccurate, see beta vs gamma radiation.
6 SAFETY
2.1
Quantitative
In a liquid scintillation assay (LSA), or liquid scintillation counting (LSC), a small aliquot, lter or swab
is added to scintillation uid and the plate or vial
counter in a scintillation counter.
A Geiger counter is a quick and rough approximation of activity. Lower energy emitters such as tritium can not be detected.
2.3
Microscopy
Scientic methods
Advantages are:
uorescence is much safer and more convenient to
use
Several uorescent molecules can be used simultaneously (given that they do not overlap, cf. FRET),
whereas with radioactivity two isotopes can be used
(tritium and a low energy isotope, e.g. 33 P due to
dierent intensities) but require special machinery
(a tritium screen and a regular phosphor-imaging
screen or a specic dual channel detector, e.g. ).
Several properties are extremely useful (cf. next section)
Note: a channel is similar to colour but distinct, it is
the pair of excitation and emission lters specic for a
dye, e.g. agilent microarrays are dual channel, working
on cy3 and cy5, these are colloquially referred to as green
and red.
Radioactivity concentration
6 Safety
If good health physics controls are maintained in a laboratory where radionuclides are used, it is unlikely that
the overall radiation dose received by workers will be of
much signicance. Nevertheless the eects of low doses
are mostly unknown so many regulations exist to avoid
unnecessary risks, such as skin or internal exposure. Due
3
to the low penetration power and many variables involved
it is hard to convert a radioactive concentration to a dose.
1 Ci of P-32 on a square centimetre of skin (through
a dead layer of a thickness of 70 m) gives 7961 rads
(79.61 grays) per hour . Similarly a mammogram gives
an exposure of 300 mrem (3 mSv) on a larger volume
(in the US, the average annual dose is 620 mrem or 6.2
mSv[2] ).
See also
Radiation poisoning
Background radiation
Uranium Medical Research Centre (UMRC)
References
9.1
Text
9.2
Images
9.3
Content license