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Investigated influencing factors and the corresponding levels (s.

Table 5)

influencing factors
1 staining time
2 destaining time
3 temperature of staining/staining solutions
4 temperature of selectrophoretic process
5 sample buffer
6 age of gels
7 gels

parameters
1h
2h
fridge
fridge
SDS
old
pre-casted

+
5h
20 h
room temperature
room temperature
LDS
new
self-casted

general Plackett-Burman schedule for 12 experiments (s. Table 4)


no.
1
2
3
4
5
6
7
8
9
10
11
12
sum +
sum total sum
difference
effect

mean
+
+
+
+
+
+
+
+
+
+
+
+

1
+
+
+
+
+
+
-

2
+
+
+
+
+
+
-

no.

mean

1
2
3
4
5
6
7
8
9
10
11
12
sum +
sum total sum
difference
effect

+
+
+
+
+
+
+
+
+
+
+
+
129.80
----129.80
129.80
21.6333

1
staining
time
+
+
+
+
+
+
69.00
60.80
129.80
8.20
1.3667

2
destaining
time
+
+
+
+
+
+
89.80
40.00
129.80
49.80
8.3000

3
+
+
+
+
+
+
-

-Galactosidase

1. Calculation of the effects

influencing factors
3
temp. of
staining
+
+
+
+
+
+
49.70
80.10
129.80
-30.40
-5.0667

Equation 2

Ex =

x(+)

N/2

x(-)

N/2

2. Calculation of the experimental error using the effects of the dummy factors
2.1. the square of dummy effects were summed up:

2.2. the variance of the effects was calculated by dividing this sum by the number of dummy ef
2.3. the standard deviation of the effects was calculated by taking the square root of the varian

3. Statistical interpretation
the Minimum Significant Factor Effect (MIN) is the limit value which represents the experimenta
and was calculated by multiplying the standard deviation with a particular t-value

t-value:
probability value :
degrees of freedom
= number of dummy factors:

1.533
0.2
4

Phosphorylase b
no.

mean

1
2
3
4
5
6
7
8
9
10
11
12
sum +
sum total sum

+
+
+
+
+
+
+
+
+
+
+
+
115.90
----115.90

1
staining
time
+
+
+
+
+
+
61.75
54.15
115.90

influencing factors
2
3
destaining temp. of
time
staining
+
+
+
+
+
+
+
+
+
+
+
+
56.60
36.30
59.30
79.60
115.90
115.90

difference
effect

115.90
19.3167

7.60
1.2667

-2.70
-0.4500

-43.30
-7.2167

1. Calculation of the effects


Equation 2

Ex =

x(+)

N/2

x(-)

N/2

2. Calculation of the experimental error using the effects of the dummy factors
2.1. the square of dummy effects were summed up:

2.2. the variance of the effects was calculated by dividing this sum by the number of dummy ef
2.3. the standard deviation of the effects was calculated by taking the square root of the varian

3. Statistical interpretation
the Minimum Significant Factor Effect (MIN) is the limit value which represents the experimenta
and was calculated by multiplying the standard deviation with a particular t-value

t-value:
probability value :
degrees of freedom
= number of dummy factors:

1.533
0.2
4

BSA
no.

mean

1
2
3
4
5
6
7
8
9
10

+
+
+
+
+
+
+
+
+
+

1
staining
time
+
+
+
+
+
+

2
destaining
time
+
+
+
+
+
-

influencing factors
3
temp. of
staining
+
+
+
+
+

11
12
sum +
sum total sum
difference
effect

+
+
108.10
----108.10
108.10
18.0167

54.10
54.00
108.10
0.10
0.0167

+
58.10
50.00
108.10
8.10
1.3500

+
43.45
64.65
108.10
-21.20
-3.5333

1. Calculation of the effects


Equation 2

Ex =

x(+)

N/2

x(-)

N/2

2. Calculation of the experimental error using the effects of the dummy factors
2.1. the square of dummy effects were summed up:

2.2. the variance of the effects was calculated by dividing this sum by the number of dummy ef
2.3. the standard deviation of the effects was calculated by taking the square root of the varian

3. Statistical interpretation
the Minimum Significant Factor Effect (MIN) is the limit value which represents the experimenta
and was calculated by multiplying the standard deviation with a particular t-value

t-value:
probability value :
degrees of freedom
= number of dummy factors:

1.533
0.2
4

Glutamic dehydrogenase
no.

mean

1
2
3
4
5

+
+
+
+
+

1
staining
time
+
+
+
+

2
destaining
time
+
+
+
+

influencing factors
3
temp. of
staining
+
+
+
-

6
7
8
9
10
11
12
sum +
sum total sum
difference
effect

+
+
+
+
+
+
+
90.75
----90.75
90.75
15.1250

+
+
42.25
48.50
90.75
-6.25
-1.0417

+
+
43.10
47.65
90.75
-4.55
-0.7583

+
+
+
45.45
45.30
90.75
0.15
0.0250

1. Calculation of the effects


Equation 2

Ex =

x(+)

N/2

x(-)

N/2

2. Calculation of the experimental error using the effects of the dummy factors
2.1. the square of dummy effects were summed up:

2.2. the variance of the effects was calculated by dividing this sum by the number of dummy ef
2.3. the standard deviation of the effects was calculated by taking the square root of the varian

3. Statistical interpretation
the Minimum Significant Factor Effect (MIN) is the limit value which represents the experimenta
and was calculated by multiplying the standard deviation with a particular t-value

t-value:
probability value :
degrees of freedom
= number of dummy factors:

1.533
0.2
4

Lactate dehydrogenase
no.

mean

1
staining
time

2
destaining
time

influencing factors
3
temp. of
staining

1
2
3
4
5
6
7
8
9
10
11
12
sum +
sum total sum
difference
effect

+
+
+
+
+
+
+
+
+
+
+
+
59.20
----59.20
59.20
9.8667

+
+
+
+
+
+
31.05
28.15
59.20
2.90
0.4833

+
+
+
+
+
+
29.45
29.75
59.20
-0.30
-0.0500

+
+
+
+
+
+
29.45
29.75
59.20
-0.30
-0.0500

1. Calculation of the effects


Equation 2

Ex =

x(+)

N/2

x(-)

N/2

2. Calculation of the experimental error using the effects of the dummy factors
2.1. the square of dummy effects were summed up:

2.2. the variance of the effects was calculated by dividing this sum by the number of dummy ef
2.3. the standard deviation of the effects was calculated by taking the square root of the varian

3. Statistical interpretation
the Minimum Significant Factor Effect (MIN) is the limit value which represents the experimenta
and was calculated by multiplying the standard deviation with a particular t-value

t-value:
probability value :
degrees of freedom
= number of dummy factors:

1.533
0.2
4

Carbonic anhydrase
no.

mean

1
2
3
4
5
6
7
8
9
10
11
12
sum +
sum total sum
difference
effect

+
+
+
+
+
+
+
+
+
+
+
+
62.25
----62.25
62.25
10.3750

1
staining
time
+
+
+
+
+
+
34.00
28.25
62.25
5.75
0.9583

2
destaining
time
+
+
+
+
+
+
34.35
27.90
62.25
6.45
1.0750

influencing factors
3
temp. of
staining
+
+
+
+
+
+
27.65
34.60
62.25
-6.95
-1.1583

1. Calculation of the effects


Equation 2

Ex =

x(+)

N/2

x(-)

N/2

2. Calculation of the experimental error using the effects of the dummy factors
2.1. the square of dummy effects were summed up:

2.2. the variance of the effects was calculated by dividing this sum by the number of dummy ef
2.3. the standard deviation of the effects was calculated by taking the square root of the varian

3. Statistical interpretation
the Minimum Significant Factor Effect (MIN) is the limit value which represents the experimenta
and was calculated by multiplying the standard deviation with a particular t-value

t-value:
probability value :
degrees of freedom

1.533
0.2

= number of dummy factors:

Trypsin inhibitor
no.

mean

1
2
3
4
5
6
7
8
9
10
11
12
sum +
sum total sum
difference
effect

+
+
+
+
+
+
+
+
+
+
+
+
80.95
----80.95
80.95
13.4917

1
staining
time
+
+
+
+
+
+
39.65
41.30
80.95
-1.65
-0.2750

influencing factors
2
3
destaining temp. of
time
staining
+
+
+
+
+
+
+
+
+
+
+
+
39.45
41.95
41.50
39.00
80.95
80.95
-2.05
2.95
-0.3417
0.4917

1. Calculation of the effects


Equation 2

Ex =

x(+)

N/2

x(-)

N/2

2. Calculation of the experimental error using the effects of the dummy factors
2.1. the square of dummy effects were summed up:

2.2. the variance of the effects was calculated by dividing this sum by the number of dummy ef
2.3. the standard deviation of the effects was calculated by taking the square root of the varian

3. Statistical interpretation
the Minimum Significant Factor Effect (MIN) is the limit value which represents the experimenta
and was calculated by multiplying the standard deviation with a particular t-value

t-value:
probability value :
degrees of freedom
= number of dummy factors:

1.533
0.2
4

Two further proteins (Myosin and Lysozym) were investigated. However, these proteins appear
areas was not possible in these cases. Therefore, these proteins were not used for the evaluat
For completeness the results of these proteins are shown in the following two tables:

Myosin
no.

mean

1
2
3
4
5
6
7
8
9
10
11
12
sum +
sum total sum
difference
sum + / n +
sum - / n effect

+
+
+
+
+
+
+
+
+
+
+
+
128.55
----128.55
128.55

21.4250

1
staining
time
+
+
+
+
+
+
62.80
65.75
128.55
-2.95
15.70
13.15
2.5500

influencing factors
2
3
destaining temp. of
time
staining
+
+
+
+
+
+
+
+
+
+
+
+
68.30
59.50
60.25
69.05
128.55
128.55
8.05
-9.55
17.08
11.90
12.05
17.26
5.0250
-5.3625

1. Calculation of the effects


Equation 2

Ex =

x(+)

N/2

x(-)

N/2

2. Calculation of the experimental error using the effects of the dummy factors

2.1. the square of dummy effects were summed up:

2.2. the variance of the effects was calculated by dividing this sum by the number of dummy ef
2.3. the standard deviation of the effects was calculated by taking the square root of the varian

3. Statistical interpretation
the Minimum Significant Factor Effect (MIN) is the limit value which represents the experimenta
and was calculated by multiplying the standard deviation with a particular t-value

t-value:
probability value :
degrees of freedom
= number of dummy factors:

Lysozym
no.

1
2
3
4
5
6
7
8
9
10
11
12
sum +
sum total sum
difference
sum + / n +
sum - / n effect

mean

+
+
+
+
+
+
+
+
+
+
+
+
60.2
----60.2
60.2

10.0333

1
staining
time
+
+
+
+
+
+
39.3
21.0
60.2
18.3
7.9
4.2
3.6600

1.533
0.2
4

2
destaining
time
+
+
+
+
+
+
20.6
39.7
60.2
-19.1
5.1
6.6
-1.4708

1. Calculation of the effects


Equation 2

Ex =

x(+)

N/2

x(-)

N/2

influencing factors
3
temp. of
staining
+
+
+
+
+
+
29.9
30.4
60.2
-0.5
6.0
6.1
-0.1000

Ex =

x(+)

N/2

x(-)

N/2

2. Calculation of the experimental error using the effects of the dummy factors
2.1. the square of dummy effects were summed up:

2.2. the variance of the effects was calculated by dividing this sum by the number of dummy ef
2.3. the standard deviation of the effects was calculated by taking the square root of the varian

3. Statistical interpretation
the Minimum Significant Factor Effect (MIN) is the limit value which represents the experimenta
and was calculated by multiplying the standard deviation with a particular t-value

t-value:
probability value :
degrees of freedom
= number of dummy factors:

1.533
0.2
4

4
+
+
+
+
+
+
-

influencing factors
4
temp. of
electroph.
+
+
+
+
+
+
70.45
59.35
129.80
11.10
1.8500

5
+
+
+
+
+
+
-

6
+
+
+
+
+
+
-

7
+
+
+
+
+
+
-

8
+
+
+
+
+
+
-

5
sample
buffer
+
+
+
+
+
+
72.15
57.65
129.80
14.50
2.4167

6
age of
gels
+
+
+
+
+
+
65.15
64.65
129.80
0.50
0.0833

7
gels

+
+
+
+
+
+
62.65
67.15
129.80
-4.50
-0.7500

+
+
+
+
+
+
70.65
59.15
129.80
11.50
1.9167

9
+
+
+
+
+
+
-

10
+
+
+
+
+
+
-

11
+
+
+
+
+
+
-

dummy factors
9
10

11

+
+
+
+
+
+
71.40
58.40
129.80
13.00
2.1667

+
+
+
+
+
+
59.15
70.65
129.80
-11.50
-1.9167

+
+
+
+
+
+
54.30
75.50
129.80
-21.20
-3.5333

The effect was calculated by determining the difference between the average
of the + and - responses Y for each independent and dummy variable.
N is the number of experiments of the design (in this case N = 12 experiments
consisting of 6 + and - experiments, respectively)

ts of the dummy factors

sum:

his sum by the number of dummy effects


taking the square root of the variance

3.6736
4.6944
3.6736
12.4844
24.5261
6.1315
2.4762

The steps 2.1. to 2.3. are summe


equation 3

e which represents the experimental variability within the design


th a particular t-value

influencing factors
4
temp. of
electroph.
+
+
+
+
+
+
50.75
65.15
115.90

equation 4
MIN =

an effect is considered as signific

MIN:
3.7965
MIN = Minimun Significant Factor Effect

5
sample
buffer
+
+
+
+
+
+
74.60
41.30
115.90

6
age of
gels
+
+
+
+
+
+
48.40
67.50
115.90

7
gels

+
+
+
+
+
+
71.05
44.85
115.90

+
+
+
+
+
+
77.65
38.25
115.90

dummy factors
9
10

+
+
+
+
+
+
55.45
60.45
115.90

+
+
+
+
+
+
57.40
58.50
115.90

11

+
+
+
+
+
+
62.25
53.65
115.90

-14.40
-2.4000

33.30
5.5500

-19.10
-3.1833

26.20
4.3667

39.40
6.5667

-5.00
-0.8333

-1.10
-0.1833

8.60
1.4333

The effect was calculated by determining the difference between the average
of the + and - responses Y for each independent and dummy variable.
N is the number of experiments of the design (in this case N = 12 experiments
consisting of 6 + and - experiments, respectively)

ts of the dummy factors

sum:

his sum by the number of dummy effects


taking the square root of the variance

43.1211
0.6944
0.0336
2.0544
45.9036
11.4759
3.3876

The steps 2.1. to 2.3. are summe


equation 3

e which represents the experimental variability within the design


th a particular t-value

influencing factors
4
temp. of
electroph.
+
+
+
+
+
+

equation 4
MIN =

an effect is considered as signific

MIN:
5.1939
MIN = Minimun Significant Factor Effect

5
sample
buffer
+
+
+
+
+
-

6
age of
gels
+
+
+
+
+

7
gels

+
+
+
+
+

+
+
+
+
+
+

dummy factors
9
10

+
+
+
+
+
+
-

+
+
+
+
+
+
-

11

+
+
+
+
+
-

49.10
59.00
108.10
-9.90
-1.6500

+
52.70
55.40
108.10
-2.70
-0.4500

+
58.80
49.30
108.10
9.50
1.5833

+
45.05
63.05
108.10
-18.00
-3.0000

66.60
41.50
108.10
25.10
4.1833

56.95
51.15
108.10
5.80
0.9667

44.90
63.20
108.10
-18.30
-3.0500

+
51.65
56.45
108.10
-4.80
-0.8000

The effect was calculated by determining the difference between the average
of the + and - responses Y for each independent and dummy variable.
N is the number of experiments of the design (in this case N = 12 experiments
consisting of 6 + and - experiments, respectively)

ts of the dummy factors

sum:

his sum by the number of dummy effects


taking the square root of the variance

17.5003
0.9344
9.3025
0.6400
28.3772
7.0943
2.6635

The steps 2.1. to 2.3. are summe


equation 3

e which represents the experimental variability within the design


th a particular t-value

influencing factors
4
temp. of
electroph.
+
+
+
-

equation 4
MIN =

an effect is considered as signific

MIN:
4.0837
MIN = Minimun Significant Factor Effect

5
sample
buffer
+
+
-

6
age of
gels
+
+

7
gels

+
-

+
+

dummy factors
9
10

+
+
+

+
+
+
-

11

+
+
+

+
+
+
40.10
50.65
90.75
-10.55
-1.7583

+
+
+
+
46.95
43.80
90.75
3.15
0.5250

+
+
+
+
49.05
41.70
90.75
7.35
1.2250

+
+
+
+
+
45.45
45.30
90.75
0.15
0.0250

+
+
+
+
54.90
35.85
90.75
19.05
3.1750

+
+
+
50.45
40.30
90.75
10.15
1.6917

+
+
+
47.80
42.95
90.75
4.85
0.8083

+
+
+
47.20
43.55
90.75
3.65
0.6083

The effect was calculated by determining the difference between the average
of the + and - responses Y for each independent and dummy variable.
N is the number of experiments of the design (in this case N = 12 experiments
consisting of 6 + and - experiments, respectively)

ts of the dummy factors

sum:

his sum by the number of dummy effects


taking the square root of the variance

10.0806
2.8617
0.6534
0.3701
13.9658
3.4915
1.8685

The steps 2.1. to 2.3. are summe


equation 3

e which represents the experimental variability within the design


th a particular t-value

influencing factors
4
temp. of
electroph.

equation 4
MIN =

an effect is considered as signific

MIN:
2.8649
MIN = Minimun Significant Factor Effect

5
sample
buffer

6
age of
gels

7
gels

dummy factors
9
10

11

+
+
+
+
+
+
29.40
29.80
59.20
-0.40
-0.0667

+
+
+
+
+
+
26.70
32.50
59.20
-5.80
-0.9667

+
+
+
+
+
+
31.75
27.45
59.20
4.30
0.7167

+
+
+
+
+
+
27.85
31.35
59.20
-3.50
-0.5833

+
+
+
+
+
+
34.55
24.65
59.20
9.90
1.6500

+
+
+
+
+
+
30.85
28.35
59.20
2.50
0.4167

+
+
+
+
+
+
29.85
29.35
59.20
0.50
0.0833

+
+
+
+
+
+
33.30
25.90
59.20
7.40
1.2333

The effect was calculated by determining the difference between the average
of the + and - responses Y for each independent and dummy variable.
N is the number of experiments of the design (in this case N = 12 experiments
consisting of 6 + and - experiments, respectively)

ts of the dummy factors

sum:

his sum by the number of dummy effects


taking the square root of the variance

2.7225
0.1736
0.0069
1.5211
4.4242
1.1060
1.0517

The steps 2.1. to 2.3. are summe


equation 3

e which represents the experimental variability within the design


th a particular t-value
equation 4
MIN =

MIN:
1.6125
MIN = Minimun Significant Factor Effect

an effect is considered as signific

influencing factors
4
temp. of
electroph.
+
+
+
+
+
+
33.65
28.60
62.25
5.05
0.8417

5
sample
buffer
+
+
+
+
+
+
30.75
31.50
62.25
-0.75
-0.1250

6
age of
gels
+
+
+
+
+
+
32.50
29.75
62.25
2.75
0.4583

7
gels

+
+
+
+
+
+
30.60
31.65
62.25
-1.05
-0.1750

+
+
+
+
+
+
37.50
24.75
62.25
12.75
2.1250

dummy factors
9
10

+
+
+
+
+
+
30.10
32.15
62.25
-2.05
-0.3417

+
+
+
+
+
+
28.70
33.55
62.25
-4.85
-0.8083

11

+
+
+
+
+
+
31.50
30.75
62.25
0.75
0.1250

The effect was calculated by determining the difference between the average
of the + and - responses Y for each independent and dummy variable.
N is the number of experiments of the design (in this case N = 12 experiments
consisting of 6 + and - experiments, respectively)

ts of the dummy factors

sum:

his sum by the number of dummy effects


taking the square root of the variance

4.5156
0.1167
0.6534
0.0156
5.3014
1.3253
1.1512

The steps 2.1. to 2.3. are summe


equation 3

e which represents the experimental variability within the design


th a particular t-value
equation 4
MIN =

MIN:

1.7651

an effect is considered as signific

MIN = Minimun Significant Factor Effect

influencing factors
4
temp. of
electroph.
+
+
+
+
+
+
36.40
44.55
80.95
-8.15
-1.3583

5
sample
buffer
+
+
+
+
+
+
42.25
38.70
80.95
3.55
0.5917

6
age of
gels
+
+
+
+
+
+
48.85
32.10
80.95
16.75
2.7917

7
gels

+
+
+
+
+
+
39.95
41.00
80.95
-1.05
-0.1750

+
+
+
+
+
+
45.05
35.90
80.95
9.15
1.5250

dummy factors
9
10

+
+
+
+
+
+
38.70
42.25
80.95
-3.55
-0.5917

+
+
+
+
+
+
34.75
46.20
80.95
-11.45
-1.9083

11

+
+
+
+
+
+
42.70
38.25
80.95
4.45
0.7417

The effect was calculated by determining the difference between the average
of the + and - responses Y for each independent and dummy variable.
N is the number of experiments of the design (in this case N = 12 experiments
consisting of 6 + and - experiments, respectively)

ts of the dummy factors

his sum by the number of dummy effects


taking the square root of the variance

sum:

e which represents the experimental variability within the design


th a particular t-value

2.3256
0.3501
3.6417
0.5501
6.8675
1.7169
1.3103

The steps 2.1. to 2.3. are summe


equation 3

equation 4
MIN =

an effect is considered as signific

MIN:
2.0090
MIN = Minimun Significant Factor Effect

ed. However, these proteins appear at the top or rather at the bottom of the gel and a correct integration of the peak
oteins were not used for the evaluation of the Plackett-Burman design.
n the following two tables:

influencing factors
4
temp. of
electroph.
+
+
+
+
+
+
51.15
77.40
128.55
-26.25
10.23
19.35
-9.1200

5
sample
buffer
+
+
+
+
+
+
56.20
72.35
128.55
-16.15
14.05
14.47
-0.4200

6
age of
gels
+
+
+
+
+
+
93.35
35.20
128.55
58.15
15.56
11.73
3.8250

7
gels

+
+
+
+
+
+
32.05
96.50
128.55
-64.45
10.68
16.08
-5.4000

+
+
+
+
+
+
64.60
63.95
128.55
0.65
16.15
12.79
3.3600

dummy factors
9
10

+
+
+
+
+
+
60.85
67.70
128.55
-6.85
15.21
13.54
1.6725

+
+
+
+
+
+
49.65
78.90
128.55
-29.25
12.41
15.78
-3.3675

The effect was calculated by determining the difference between the average
of the + and - responses Y for each independent and dummy variable.
N is the number of experiments of the design (in this case N = 12 experiments
consisting of 6 + and - experiments, respectively)

ts of the dummy factors

11

+
+
+
+
+
+
67.85
60.70
128.55
7.15
13.57
15.18
-1.6050

11.2896
2.7973
11.3401
2.5760
28.0029
7.0007
2.6459

sum:

his sum by the number of dummy effects


taking the square root of the variance

The steps 2.1. to 2.3. are summe


equation 3

e which represents the experimental variability within the design


th a particular t-value
equation 4
MIN =

an effect is considered as signific

MIN:
4.0567
MIN = Minimun Significant Factor Effect

influencing factors
4
temp. of
electroph.
+
+
+
+
+
+
37.4
22.8
60.2
14.6
7.5
4.6
2.9200

5
sample
buffer
+
+
+
+
+
+
36.6
23.7
60.2
12.9
6.1
5.9
0.1792

6
age of
gels
+
+
+
+
+
+
27.2
33.0
60.2
-5.8
5.4
6.6
-1.1600

7
gels

+
+
+
+
+
+
30.2
30.1
60.2
0.1
5.0
7.5
-2.4875

+
+
+
+
+
+
22.6
37.6
60.2
-15
5.7
6.3
-0.6167

dummy factors
9
10

+
+
+
+
+
+
25.6
34.7
60.2
-9.1
5.1
6.9
-1.8200

The effect was calculated by determining the difference between the average

+
+
+
+
+
+
24.8
35.5
60.2
-10.7
5.0
7.1
-2.1400

11

+
+
+
+
+
+
23.6
36.7
60.2
-13.1
5.9
6.1
-0.2208

of the + and - responses Y for each independent and dummy variable.


N is the number of experiments of the design (in this case N = 12 experiments
consisting of 6 + and - experiments, respectively)

ts of the dummy factors

sum:

his sum by the number of dummy effects


taking the square root of the variance

0.3803
3.3124
4.5796
0.0488
8.3210
2.0803
1.4423

The steps 2.1. to 2.3. are summe


equation 3

e which represents the experimental variability within the design


th a particular t-value
equation 4
MIN =

MIN:
2.2114
MIN = Minimun Significant Factor Effect

an effect is considered as signific

response

response
RSD%

19.5
6.6
9.0
11.5
17.7
6.9
5.3
7.0
23.3
6.8
8.9
7.4

The steps 2.1. to 2.3. are summerized in

2
(Di)

an effect is considered as significant at a given if

Ex

> MIN

response
RSD%

7.8
5.0
5.0
4.2
12.0
26.2
6.4
7.9
20.0
6.6
7.7
7.2

The steps 2.1. to 2.3. are summerized in

2
(Di)

an effect is considered as significant at a given if

Ex

> MIN

response
RSD%

9.0
5.8
7.7
4.0
18.5
8.1
5.3
10.9
12.6
8.8

6.4
11.2

The steps 2.1. to 2.3. are summerized in

2
(Di)

an effect is considered as significant at a given if

Ex

> MIN

response
RSD%

4.3
5.4
7.4
6.1
10.2

9.4
7.9
12.8
8.2
7.0
7.0
5.3

The steps 2.1. to 2.3. are summerized in

2
(Di)

an effect is considered as significant at a given if

Ex

> MIN

response
RSD%

3.7
4.6
6.3
3.8
8.0
5.4
5.3
5.4
3.9
5.7
3.8
3.5

The steps 2.1. to 2.3. are summerized in

2
(Di)

an effect is considered as significant at a given if

Ex

> MIN

response
RSD%

6.1
4.0
5.9
3.3
8.1
5.8
3.8
3.9
7.1
6.8
3.9
3.7

The steps 2.1. to 2.3. are summerized in

2
(Di)

an effect is considered as significant at a given if

Ex

> MIN

Ex

> MIN

response
RSD%

5.5
5.6
5.2
3.2
9.9
6.5
5.5
8.9
5.7
9.0
10.1
6.0

The steps 2.1. to 2.3. are summerized in

2
(Di)

an effect is considered as significant at a given if

Ex

> MIN

on of the peak

response
RSD%

16.1
8.4
9.3
N.N.
28.2
N.N.
7.3
17.0
N.N.
10.1
14.7
17.5

The steps 2.1. to 2.3. are summerized in

2
(Di)

an effect is considered as significant at a given if

Ex

> MIN

response
RSD%

9.0
11.2
N.N.
3.8
N.N.
6.1
3.3
2.6
4.7
9.3
3.1
7.3

The steps 2.1. to 2.3. are summerized in

2
(Di)

an effect is considered as significant at a given if

Ex

> MIN