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Certain plants products widely used in the traditional medicine system of India were studied for
immunostimulant activity. In the present study, strong stimulation of antigen specific and non specific
immunity was evidenced by increase in haemagglutinating antibody (HA) titre, plaque forming cells
(PFC) and in macrophage migration index (MMI), in mice fed with 50% ethanolic extract of these plants.
The observation provides scientific basis for wide spread use of these plant products which are likely
to play a role in Indian traditional medicine as tonic for rejuvenation therapy and chronic ailments.
Key words: Macrophage migration index, haemagglutinating antibody, immunostimulant.
INTRODUCTION
Immunostimulant activity has been demonstrated in
several plants used in the traditional medicine of India,
China and European countries for rejuvenation therapy
and treatment of chronic ailments (Atal et al., 1986;
Wagner, 1983, 1984a, b). Strong immunostimulant
activity has been reported from this laboratory in Panax
pseudoginseng (Araliaceae) (Dua et al., 1989), Picrorhiza
kurroa (Scrophulariacae) (Puri et al., 1992), Andrographis
paniculata (Acanthaceae) (Puri et al., 1993), Nyctanthes
arbortristis L. (Nyctanthaceae) (Puri et al., 1994),
Curculigo orchioides (Hypoxidaceae) (Lakshmi et al.,
2004), and certain dry fruits and plant material, namely:
Prunus
amygdalus
(Rosaceae),
Euryale
ferox
(Nymphaceae), Buchanania lanzan (Anacardiaceae),
Phoenix dactylifera (Arecaceae) and Zingiber officinale
(Zingiberaceae) given to mothers after child birth and to
invalids in both of whom the immune status is low (Puri et
al., 2000).
In addition to the immunostimulant activity, P. kurroa
Puri et al.
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Table 1. Cell mediated and humoral immune response in certain plants used in Indian system of medicine.
S/No.
Plants
Cell (MMI)
mediated immune
response
Tinospora cordifolia
3.2
HA titre
meanSD
614.4228.97
Achyranthus asper
3.0
716.8280.4
12614.47a
Tephrosia purpurea
2.9
1228.8457.94b
9017.67
Ocimum- sanctum
2.9
1433.6560.8a
13023.71a
1433.6560.8a
13021.5a
9619.18a
Tonic, fever, hepatitis, spleen, enlargement, tetanus, prevents ageing and premature graying of hears.
Antitubercular, jaundice, antiseptic bronchitis
Bitter tonic, general debility, febrifuge anti-rheumatic antigonorrhoeic, jaundice, antileprotic, skin disorder.
Tonic, anti asthmatic, anti rheumatics, bronchitis, diseases of lung and chest, spleen enlargement, liver diseases,
antigonorrhoeic, anti syphilitic eczema
5
6
Eclipta alba
Luffa echinata
2.9
2.8
1228.8457.94b
Evolvulus alisinoides
2.3
1433.6560.8a
12012
8
9
Amoora rohituka
Cinnamon tamala
2.3
2.0
614.4228.97
307.2114.48
8016.55
6510.09
10
Cichorium intybus
2.0
358.4140.21
8017.13
11
12
Zizyphus sativa
Zingiber officinale
1.5
1.3
460.8114.48
1443.6560.8a
9018.54
16048.08a
13
Foeniculum vulgare
1.2
1228.8457.94b
10513.17b
14
Cassia occidentalis
1.0
1638.4560.86a
19022.06a
15
16
Pureria tuberosa
Memordica diocea
1.0
1.0
1443.6560.86a
1228.8457.94b
15035.06a
12514.88a
17
Solanum nigrum
0.9
716.8280.4
12012.74a
18
19
Hedychium spicatum
Control
0.8
1.0
912.6228.97
307.2114.48
9619.17
6515.47
Puri et al.
3245
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