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HORTICULTURAL ENTOMOLOGY
ABSTRACT Floating row covers or insect-proof nets with ne mesh are effective at protecting
vegetable crops against aphids but negatively impact plant health, especially under warm conditions.
Furthermore, in control of cabbage insect pests, aphid parasitoids cannot enter the ne-mesh nets,
leading to frequent aphid outbreaks. To surmount these difculties, a 40-mesh-size repellent net
treated with alphacypermethrin was studied in laboratory and eld tests. Results showed both irritant
and repellent effects of the alphacypermethrin-treated net on Myzus persicae (Sulzer) (Hemiptera:
Aphididae) and its parasitoid Aphidius colemani (Haliday) (Hymenoptera: Braconidae). Under eld
conditions, there were no pests on cabbage protected with the repellent net. The repellent net allowed
combining a visual and repellent barrier against aphids. Because of this additive effect, repellent nets
allowed covering cabbage permanently with adequate protection against all pests.
KEY WORDS repellent netting, alphacypermethrin, Myzus persicae, Aphidius colemani, cabbage
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August 2013
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Fig. 1. Contact toxicity of the alphacypermethrin-treated net (AgroNet 0.9) on winged aphids. Aphids from Myzus persicae
and Lipaphis erysimi were compared with the nontreated net (AgroNet 0.9NT). Percentage of mortality with 95% CIs.
Results
Toxic Effect. Contact toxicity test in Munger cells
(Fig. 1) showed a signicant toxicity (P 0.047) of the
alphacypermethrin-treated net (80% mortality) on
Fig. 2. Contact toxicity of cypermethrin on winged Myzus persicae and Lipaphis erysimi from eld populations.
Percentage of mortality with 95% CIs.
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AgroNet 0.9
AgroNet 0.9NT
P-value*
20
20
Mobility
(s SE)
Distance
(mm SE)
Speed
(mm/s SE)
517 (27)aa
342 (41)b
0.0005
245 (20)a
172 (16)b
0.008
0.32 (0.03)a
0.22 (0.02)b
0.0008
AgroNet 0.9
AgroNet 0.9NT
P value*
34
34
Time
(s SE)
Speed
(mm/s SE)
Distance
(mm SE)
338 (49)aa
562 (49)b
0.011
0.37 (0.04)
0.33 (0.03)
0.252
104 (14)a
139 (15)b
0.029
a
Different letters represent statistically signicant differences at
0.05.
* Statistical analysis with MannWhitney test.
AgroNet 0.9
AgroNet 0.9NT
P value*
500
500
M. persicae
(n SE)
A. colemani
(n SE)
6.00% (0.09)a
16.00% (0.27)b
0.017
0.88% (0.07)
1.40% (0.11)
0.098
The aphid B. brassicae was observed mainly on cabbage in nonprotected plots (control). The infestation
of B. brassicae began 2 wk after planting out and
increased to a maximum of 300 aphids per 21 cabbages,
observed the rst of July. Then the infestation decreased gradually until harvest. The differences of
aphid numbers between no net in the control and the
three AgroNets were statistically signicant (Table 4).
Few B. brassicae (0.2/plant) were observed on cabbage protected by the AgroNet 0.9NT. However, no B.
brassicae was observed on cabbage under the AgroNet
0.9 or AgroNet 0.4NT.
The green aphids, M. persicae and L. erysimi, were
observed 2 wk after planting on the nonprotected
cabbage and on cabbage protected with the nontreated nets (Fig. 3). No green aphid was observed on
cabbage protected with the treated net. The outbreak
increased on cabbage under the nontreated nets compared with the nonprotected cabbage. In addition, as
the net mesh size increased, so did the aphid outbreak.
The ANOVA on the cumulated data of 10 sampling
dates showed a signicant difference of green aphids
between the treatments. From the highest to the lowest green aphid infestations, the treatments were
ranked as follows: AgroNet 0.9NT, AgroNet 0.4NT,
nonprotected control, and AgroNet 0.9 (Table 4).
The outbreak of green aphids disappeared 2 wk before
harvest in all treatments.
Parasitized aphids observed as aphid mummies
were mainly observed on nonprotected cabbage (control), suggesting an exclusion of benecial insects by
nets, especially with ner mesh (Table 4). The maximum proportion of parasitized aphids (mummies)
was 25.6%, observed at the peak of aphid outbreak. A
few parasitized aphids were also observed on cabbage
protected by the nontreated larger-mesh-size net,
with a maximum of 4.6% aphid mummies. Practically
no parasitized aphids were observed on cabbage protected by the nontreated lower-size mesh. As there
was no aphid on cabbage protected by AgroNet 0.9,
there were no aphid mummies. The statistical analysis
on the mean of parasitized aphids showed a higher
parasitism with the control than with the nets (Table
4). The average of parasitized aphids was also significantly higher with the AgroNet 0.9NT than with the
AgroNet 0.4NT.
Cabbage were harvested per plot, counted, and
weighted individually. The low yield in all treatments
was because of small snails coming from under the
plastic mulch, which damaged the plants in all plots 7 d
August 2013
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Table 4. Cumulative data of Plutella xylostella larva, Brevicoryne brassicae, green aphids (gathering Myzus persicae and Lipaphis
erysimi) and mean rate of parasitized aphids over the 10 sampling dates on cabbage covered permanently with an alphacypermethrintreated net (AgroNet 0.9) or nontreated nets (AgroNet 0.9NT and AgroNet 0.4NT) in comparison with a nonprotected control
Treatments
P. xylostella
N/plant (SE)
B. brassicae
N/plant (SE)
Green aphids
(N/plant SE)
Parasitized aphids
(% SE)
Marketable cabbagea
(% SE)
Control
AgroNet 0.4NT
AgroNet 0.9NT
AgroNet 0.9
F
P value
1.04 (0.38)ab
0.02 (0.02)b
0.0b
0.0b
4.12
0.03
49.3 (9.53)a
0.0b
0.18 (0.05)b
0.0b
15.25
0.001
19.8 (12.8)b
55.4 (17.7)ab
114.8 (59.4)a
0.0c
3.46
0.05
8.42 (1.47)a
0.02 (0.01)c
1.35 (0.34)b
0.0c
9.84
0.001
14.3 (11.3)a
33.3 (6.4)ab
27.4 (9.0)ab
56.0 (6.3)b
4.09
0.03
a
b
Fig. 3. Dynamic of aphid infestation on cabbage. Number of aphids (SE) of Brevicoryne brassicae, Myzus persicae, and
Lipaphis erysimi observed on 21 cabbages covered permanently with an alphacypermethrin-treated net (AgroNet 0.9) or
a nontreated net (AgroNet 0.9NT or AgroNet 0.4NT) in comparison with a nonprotected control.
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August 2013
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Received 4 January 2013; accepted 13 March 2013.