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Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Laboratory of Lipids Corps, National Institute of Research and Physico-Chemical Analysis (INRAP), Technical Pole, 2020 Sidi Thabet, Ariana, Tunisia
a r t i c l e
i n f o
Article history:
Received 18 January 2010
Received in revised form 24 May 2010
Accepted 25 May 2010
Keywords:
Phoenix canariensis
Date seed oil
Fatty acid composition
DSC
Chemical and physical parameters
a b s t r a c t
Studies were conducted on properties of seeds and oil extracted from fully ripened Phoenix canariensis
date seeds. The percentage composition of the P. canariensis seeds found is: ash 1.18%, oil 10.36%, protein
content 5.67%, total carbohydrate 72.59% and moisture 10.20%. The major nutrients (mg/100 g of oil)
determined were: potassium (255.43), magnesium (62.78), calcium (48.56) and phosphorus (41.33). The
physicochemical properties of the oil observed include: the saponication number 191.28; the iodine
number 76.66, the p-anisidine value 3.67; the peroxide value 3.62 meq/kg; the unsaponiable matter
content 1.79%, the free fatty acids content 0.59%; the carotenoid content 5.51 mg/100 g; the chlorophyll
content 0.10 mg/100 g and the refractive index 1.45. The main fatty acids of oil were oleic (50.10%),
linoleic (19.23%), lauric (10.24%). palmitic (9.83%) and stearic (7.51%). The main triacylglycerols found in
P. canariensis seed oil were: LaMM + LaLaP (18.9%), LaMP + MMM (15.31%) and LaOO + PLL + MPL (12.86%).
The DSC melting curves revealed that: melting point = 3.71 C and melting enthalpy = 62.08 J/g. The sterol
marker, -sitosterol, accounted for 76.06% of the total sterols content in the seed oil followed by campesterol (8.89%) and 5 avenesterol (8.79%). -Tocotrienol was the major tocol (66%) with the rest being
-tocotrienol and -tocopherol.
2010 Elsevier B.V. All rights reserved.
1. Introduction
Much attention has recently been focused on the seed oil from
different vegetable species such as Moringa oleifera (Lalas and
Tsaknis, 2002), hemp (Oomah et al., 2002), prickly pear (Ennouri
et al., 2005), Pinus pinea L. (Nasri et al., 2005), Bauhinia purpurea
L. (Ramadan et al., 2006), Echinacea (Oomah et al., 2006), Maclura
pomifera (Fatnassi et al., 2009), Spartium junceum L. (Cerchiara et al.,
2010). Scientists of various specialities invest to highlight their new
product and its industrial application as cooking oil or for pharmaceutical and cosmetic purposes and nowadays as bio-fuel sources
(Giannelos et al., 2005; Ramadhas et al., 2005).
To our knowledge so far nobody has investigated the potencies
of the Phoenix canariensis date seed oil. The mean date seed oils
extracted and studied were from different species of the Phoenix
Dactylifera genus (Al-Showiman, 1990; Devshony et al., 1992; AlShahib and Marshall, 2003; Besbes et al., 2004, 2005).
Phoenix is the family name of 17 of palm species (canariensis,
dactylifera, reclinata, sabal, rupicola,. . .) (Burnie et al., 2006). It is a
member of the palmae family. Its native to the Canary Islands which
are located in the Atlantic Ocean off the coast of northeast Africa.
361
Table 1
Norm of chemical parameters of Phoenix canariensis.
Parameter
Norm
ISO 3657
ISO 6885
ISO 3960
ISO 660
ISO 3961
ISO 3596
362
Table 2
Chemical composition, physical and chemical properties from seeds and seed oil of
Phoenix canariensis.
Component
Chemical composition of seeds
Moisture content (%)
Oila dry matter (%)
Ash dry matter (%)
Proteinb
Carbohydratec
Potassiumd
Magnesiumd
Calciumd
Phosphorusd
Sodiumd
Irond
10.20 0.25
10.36 0.29
1.18 0.02
5.67 0.15
72.59 0.28
255.43 0.02
62.78 0.18
48.56 0.56
41.33 0.66
8.77 0.22
3.21 0.34
191.28 0.50
76.66 0.28
0.59 0.02
3.67 0.18
3.62 0.56
1.79 0.22
1.456 0.01
5.51 + 0.03
0.10 + 0.02
Liquid
a
b
c
d
are comparable with those of the date pits of P. dactyliferia L. family reported by Besbes et al., 2004. The date seeds also contained
signicant amount of important minerals (Table 2). The potassium
concentration was the highest, followed in descending order by
magnesium, calcium, phosphorus, sodium and iron. Such a chemical composition reveals the valuable potencies of such a date
seeds.
3.2. Physical properties of date seed oil
At room temperature P. canariensis seed oil is a yellow liquid
having refractive index of 1.456 (Table 2). Concerning the thermal prole, the DSC method is used to study different triglycerides
formed in P. canariensis seed oil and the thermogram seemed
to correspond to one triglyceride (Fig. 1). P. canariensis seed oil
exhibited a melting peak (3.71 C), a melting enthalpy (62.08 J/g)
and an onset temperature (13.64 C). The melting point of P.
canariensis seed oil is higher than M. pomifera (Fatnassi et al., 2009).
This difference is likely due to P. canariensis seed oils high saturated fatty acids content compared to the M. pomifera seed oil
(Table 3).
Crude P. canariensis seed oil exhibited an absorbance in the
UV-C (100290 nm) and UV-B (290320 nm) range (Fig. 2). Thus,
P. canariensis seed oil may be used in formulation of UV protectors that provide protection against both UV-B and UV-A. The
optical transmission of P. canariensis seed oil, especially in the
UV range (290400 nm) is comparable to that of raspberry seed
oil (Oomah et al., 2000) and M. pomifera seed oil (Fatnassi et al.,
2009).
The low absorbance at 232 nm (0.35) can be explained by the
presence of a minute quantity of hydroperoxides of the linoleic acid
and other decomposition products. However, the weak absorbance
at 268 nm (0.060) indicates the absence of the secondary products of oxidation and specially -diketones or -unsaturated
ketones. These results are in agreement with the low values of
the peroxide and p-anisidine values (Table 2). Green pigments,
particularly chlorophyll content, usually measured at 630, 670
363
Table 3
Fatty acid composition of Phoenix canariensis seed oil (%).
Fatty acid
Composition (%)
Saturated
C8:0
C10:0
C12:0
C14:0
C15:0
C16:0
C17:0
C18:0
C20:0
C22:0
C23:0
C24:0
0.08 0.02
0.11 0.03
10.24 0.12
7.51 0.10
0.07 0.02
9.83 0.12
0.15 0.05
1.66 0.03
0.19 0.08
0.09 0.01
0.02 0.01
0.02 0.01
Monoinsaturated
C15:1
C16:1 9
C16:1 7
C17:1
C18:1 9
C18:1 11
C20:1 11
C22:1
0.03 0.01
0.07 0.02
0.06 0.01
0.08 0.02
50.00 0.39
0.10 0.03
0.32 0.04
0.02 0.01
Polyinsaturated
C18:2
C18:3
SAFA
MUFA
PUFA
U/S
19.23 0.29
0.11 0.03
29.97 0.60
50.69 0.53
19.34 0.32
2.33
Fig. 2. Ultra violet/visible spectra of Phoenix canariensis seed oil (gure derived from
scans). ( = 200290 nm) of oil diluted 1:1000; from scans ( = 290400 nm) of oil
diluted 1:100 and from scans ( = 400800 nm) of oil diluted 1:10, all in hexane.
364
Table 4
Triacylglycerols composition of Phoenix canariensis seed oil.
Triacylglycerol
ECN
LaLaLa
LaLaM
LaLaO
LaMM + LaLaP
LLL
LaMO
LaMP + MMM
OLL
LaPO
LaPP
LaOO + PLL + MPL
OOL
PPL
MPP
POL + MOP + MOO + SLL
OOO
POO
POP
SOO
POS
36
38
40
40
42
42
42
44
44
44
44
46
46
46
46
48
48
48
50
50
2.11
4.64
1.04
18.90
0.67
4.98
15.31
1.29
11.33
0.34
12.86
2.44
4.63
0.33
6.82
3.77
3.81
2.05
1.08
1.06
Table 5
Tocols and phytosterols contents of Phoenix canariensis seed oil
(mg/100 g oil).
Compound
mg/100 g oil
-Tocopherol
-Tocopherol
-Tocopherol
-Tocopherol
-Tocotrienol
-Tocotrienol
0.61
0.92
10.30
1.03
34.01
4.63
Total
51.54
Cholesterol
Campesterol
Stigmasterol
-Sitosterol
5-Avenasterol
5,24-Stigmastadienol
7-Stigmastenol
7-Avenasterol
1.42 0.01
29.90 0.12
3.69 0.03
255.63 0.42
29.56 0.41
9.20 0.09
2.68 0.01
3.99 0.03
Total
336.07
4. Conclusion
Considering the protein, fat, mineral and carbohydrate contents
of P. canariensis seed, we can conclude that date pits could be used
to meet part of the nutritional requirements of animal feeds.
P. canariensis seed oil is extracted by hexane solvent. The unique
fatty acid and tocols composition, high absorbance of UV light, and
other desirable physicochemical characteristics indicate potential
uses of P. canariensis seed oil in food, pharmaceutics, cosmetics and
other non-food industries. However, the safety of this oil must be
tested before use for human nutrition. The production of oil from
P. canariensis seed provides the use of renewable resource, and at
the same time adding value to agricultural products.
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