Escolar Documentos
Profissional Documentos
Cultura Documentos
2014
Journal of Biodiversity and Environmental Sciences (JBES)
ISSN: 2220-6663 (Print) 2222-3045 (Online)
Vol. 5, No. 6, p. 190-199, 2014
http://www.innspub.net
RESEARCH PAPER
OPEN ACCESS
Abstract
A variability genetic analysis was carried out among Minthostachys verticillata populations collected in the
central and northwest region of Argentina. 93 plants from nine locations were analyzed by PCR, developing ESTSSR primers from the database of Mentha spp. AMOVA analysis revealed that variability was higher among
populations than within them (93% vs. 7%).The highest percentage of polymorphic loci corresponded to the
locations from Padre Monti (75%) and Cortaderas (70.83%). Three groups were identified by cluster analysis
following a geographic gradient. The genetic variability found in this study is greater than the phytochemical
variability represented in previous studies.
*Corresponding
development
is
very
demanding
in
time,
sequences
soft
herbs
While
terpenoids,
drinks,
there
several
are
teas
many
and
aromatic
studies
about
the
(ESTs)
markers
demonstrating
available
the
in
public
potential
of
genetic
within
heterogeneity
of
individuals
et al., 2010).
In the present study, we employed EST-SSR markers
The study of genetic variability by using molecular
of Argentina.
Plant material
2010).
San Luis
Cordoba
Tucuman
Populations
Geoposicion
Altitude (masl)
Latitude
Longitude
Cortaderas (COR)
22
32 29,169 S
64 58,472 O
1047
11
32 19,164 S
64 58,824 O
1094
Hornillos (HOR) 7
31
54,227 S
64 58,706 O
1084
Chacras1 (CHA1)
32 13,417 S
65 25,769 O
1000
Chacras2 (CHA2)
32 13,549 S
65 00,525 O
941
Embalse (EMB)
32 11,154 S
64 23,368 O
526
Unquillo (UNQ)
31 11,534 S
64 21,767 O
800
Ongamira (ONG)
10
30 46,013 S
64 27,656 O
1277
17
26 29,377 S
64 59,521 O
982
P
M
O
O
HU
N
C
N
CO G E
PH
Q
CHR M
M
OAA B
A
R12
(You
et
al.,
with
50%
as
optimum.
The
others
were
synthesized
in
Bio-Synthesis,
Inc.
that
were
classified
as
generated.
polymorphic,
The
highest
percentage
of
polymorphic
loci
non-reproducible.
Genetic
include
diversity
parameters
analyzed
shortest
genetic
distance
corresponds
to
the
(Fig. 2).
populations
From the twenty primers that were used, five showed
%P
Na
Ne
He
COR
70,83
1,458
1,174
0,201
0,117
HOR
25,00
0,625
1,113
0,118
0,075
CHA1
45,83
1,000
1,147
0,174
0,105
PMA
58,33
1,208
1,188
0,220
0,132
PMO
75,00
1,500
1,289
0,290
0,182
ONG
50,00
1,042
1,210
0,212
0,134
CHA2
20,83
0,500
1,091
0,088
0,056
EMB
8,33
0,333
1,059
0,050
0,035
UNQ
37,50
0,833
1,128
0,143
0,087
TOTAL
43,52
0,944
1,155
0,166
0,102
COR
HOR
CHA1
PMA
PMO
COR
****
0.9786
0.9829
0.9936
HOR
0.0217
****
0.9684
0.9644
CHA1
0.0172
0.0321
****
PMA
0.0065
0.0363
PMO
0.0677
ONG
ONG
CHA2
EMB
UNQ
0.9346 0.9633
0.9887
0.9383
0.9893
0.9069 0.9559
0.9686
0.9596
0.9899
0.9863
0.9239 0.9694
0.9852
0.9340
0.9910
0.0138
****
0.9339 0.9661
0.9910
0.9274
0.9836
0.0977
0.0792
0.0684
****
0.9223
0.8699
0.9278
0.0373
0.0451
0.0311
0.0345
0.0363 ****
0.9621
0.9263
0.9738
CHA2
0.0114
0.0319
0.0149
0.0091
0.0809 0.0386
****
0.9263
0.9876
EMB
0.0637
0.0412
0.0683
0.0754
0.1394
0.0765
0.0765
****
0.9527
UNQ
0.0107
0.0101
0.0090
0.0166
0.0749 0.0265
0.0125
0.0484
****
0.9644
df
SS
MS
Est. Var.
Among Population
54,051
6,756
0,300
7%
Within Population
84
316,390
3,767
3,767
93%
Total
92
370,441
4,067
100%
df= degrees of freedom, SS= Sum of Squares, MS= Mean Squares, Est. Var.= estimate of variance, %= percentage
of total variation.
distances
among
populations
of
M.
verticillata.
Discussion
Genetic diversity and differentiation
The microsatellite markers developed in peperina
from Mentha spp., generated polymorphic bands in
spite of being species of different genus, which
allowed
us
differentiate
the
nine
populations
principal axes.
Conclusions
Acknowledgement
The authors thank the financial support of the
1106094
gradient
Agropecuaria (INTA).
in
which
the
closest
populations
of
Instituto
Nacional
de
Tecnologia
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