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Abstract
Motile Aeromonas is one of several causes of
septicemia disease at fish. Cultivation activity does
not considering suitability factor, whether fish
density, food and environment condition that may
increase stress at fish which later decrease
immunity, therefore it is very easy for pathogen to
infect it. The purpose of this research is detecting
the existence of Motile Aeromonas and observing
colony morphology characteristics, cell and its
physiology. Isolation from fish ulcer at TSA media,
acid and gas that resulted from glucose metabolism
and positive motility test indicates the existence of
these genus and the result will then followed by
several physiologic test, colony with white yellow
color at TSA media and forming colony with
yellow at TCBS, and it is the characteristics of
Aeromonas genus colony. This bacteria is including
in proteolotic bacteria that signed with gelatinase
enzyme that being result in, and this enzyme is able
to decipher gelatin as the biggest compound in fish
scales, therefore it gives easiness on infection
process. Treatment with novobiocin antibiotic,
zone average obstruct of these genus <21mm
(Barry table), thus it can be concluded that this
bacteria is resistant to novobiocin.
Keywords : Motile Aeromonas,
characterization, goldfish
1
2.1
Clinical symptom observation and
infected fish behavior
Early detection is performed by observing the most
prominent clinical symptom that is ulcer and gold
fish behavior that directed to Motile Aeromonas
infection.
2.2
Water quality measurement
Water quality measurement includes temperature,
pH, and soluble oxygen.
2.3
Isolation and detection of Motile
Aeromonas existence
Isolation comes from ulcer that assumed infected
by Motile Aeromonas at TSA media that already
incubated 48 hours at 25C in temperature. At TSA
media, the color of bacteria colony is the white and
yellow, round, and flat edge, negative gram, shaft
cell, no spore (Holt et al., 1994). If there is colony,
then there will be identification with biochemical
testing to make sure that the colony is Motile
Aeromonas. Biochemical testing is performed by
using TSIA media, Sulfide Indol Motility, Simon
sitrate and urease agar.
isolation,
Introduction
2.4
Sensitivity Test at Novobiocin
Muller Hinton media is used for sensitivity testing
on novobiocin. Light zone diameter is compared
with Barry table to find out its sensitive or resistant
nature.
3
Results
Methodology
Agmaza martapija, Detection of Motile Aeromonas Existence at Golden Fish and Its Sensitivity on
Novobiocin
oxygen content, nutrient deficiency and pollution
and general condition that support the growth of
this bacteria (Post, 1983).
Temperatur
e
pH
DO
270C
6,4
7,35
Loc.
2
280C
6,5
7,6
Loc.
3
280C
6,3
7,85
Average
27,70C
6,4
7,6
Conclusions
B002
Parameter
3rdInternationalConferencesandWorkshopsonBasicandAppliedSciences2010ISBN:9789791909617
B002