Escolar Documentos
Profissional Documentos
Cultura Documentos
Weblinks:
1) McGraw Hill RNAi animation
http://highered.mheducation.com/sites/9834092339/student_view0/chapter16/rna_interfer
ence.html
2) Epigenetics NOVA video at https://youtu.be/M4boKud1MRk
3) RNAi NOVA video at https://youtu.be/uiObPBeof5s
March 2015
March 2015
mRNA degradation
Post-translational regulation
Protein processing and degradation (see Fig. 18.14)
Role of ubiquitin and proteasome
Role of noncoding RNAs
siRNAs
RNAi (RNA interference- see figure 18.15)
Role of Dicer
Results in either mRNA degradation or blockage of Translation
Differentiation
Know what it is
Know what morphogenesis means
Role of signals in embryonic development: (see p. 367-68)
cytoplasmic determinants
extracellular signals lead to induction
Pattern formation in drosophila
Role of nurse cells and follicle cells
Maternal effect genes: General Role and effect of mutations in:
a) Bicoid (Bicoid mRNA ( a morphogen involved in axis establishmentconcentrated in anterior end)
b) Nanos (establishes posterior structures)
Zygotic Genes: General Role and effect of mutations in:
Gap Genes, Pair-Rule Genes, Segment Polarity Genes,
Homeotic Genes
Be able to interpret graph of Anterior-Posterior Gradient involving the Maternal
effect (Bicoid and Nanos) and Zygotic Genes (Hunchback and Caudal)
Know that Maternal Effect Genes and Zygotic Genes are transcription factors that
activate each subsequent set of genes. (Know hierarchy)
Evolutionary conservation of HOX genes across many species
Role of HOX genes
Mutations in HOX genes
Relationship between location of HOX genes on chromosome and order of
anatomical structure in the animal
Cancer (Section 18.5- See Figure 18.24)
Oncogenes
Proto-oncogenes
3 ways that can transform proto-oncogenes into onco-genes
Role of ras gene and effect of mutations in ras (normal ras relies on external signal,
normally a growth factor)
March 2015
Role of p53 gene and effect of mutation in p53 (activated when it receives signal from
within nucleus DNA damage)
March 2015
Purpose
Role of:
Electricity,
Charge,
Size,
Reference Bands,
Resolution,
Apparatus (Agarose Gel, Buffer, Tracking Dye, DNA cut with different enzymes,
Wells)
PCR- purpose; materials needed; general steps; (See Figure 20.8)
Restriction Length Polymorphisms (see handout)
VNTRs (Variable Number of Tandem Repeats)
STRs (Short Tandem Repeats)
DNA fingerprinting analysis of DNA banding pattern from gel (See PowerPoint)
Use of 13 Loci in FBI database (CODIS)
Effect of Mutation at restriction site on banding patterns (Ex: Sickle v. Normal Hemoglobin- See
Figure 20.10)