Materials & Streams Report

October 21, 2014

for insulferm1
1. OVERALL PROCESS DATA
Annual Operating Time
Recipe Batch Time
Recipe Cycle Time
Number of Batches per Year
MP = Undefined

7,805.93h
147.28h
147.28h
53.00

- Page 1 -

2.1 STARTING MATERIAL REQUIREMENTS (per Section)

Section

Starting Material Active Product

Amount
Needed
(kg
Sin/kg
MP)

Main Section

(none)

Unknown Unknown Unknown Unknown

(none)

Molar
Yield
(%)

Mass
Yield
(%)

Gross
Mass
Yield
(%)

Sin = Section Starting Material, Aout = Section Active Product

2.2 BULK MATERIALS (Entire Process)

Material
Water
Glucose
Sodium Chloride
Air
Ammonia
TOTAL

kg/yr

kg/batch

2,599,887
259,170
23,638
1,208,400
24,910
4,116,005

49,054.465
4,890.000
446.000
22,800.000
470.000
77,660.465

kg/yr

kg/batch

2,599,887
259,170
23,638
1,208,400
24,910
4,116,005

49,054.465
4,890.000
446.000
22,800.000
470.000
77,660.465

kg/kg MP

2.3 BULK MATERIALS (per Section)

SECTIONS IN: Main Branch
Main Section
Material
Water
Glucose
Sodium Chloride
Air
Ammonia
TOTAL

kg/kg MP

- Page 2 -

2.4 BULK MATERIALS (per Material)

Water
Procedure
Main Section (Main Branch)
P-1
P-6
P-8
TOTAL

Glucose
Procedure
Main Section (Main Branch)
P-1
TOTAL

Sodium Chloride
Procedure
Main Section (Main Branch)
P-1
TOTAL

Air
Procedure
Main Section (Main Branch)
P-3
TOTAL

Ammonia
Procedure
Main Section (Main Branch)
P-4
TOTAL

% Total

kg/yr

kg/batch

76.92
9.27
13.80
100.00

1,999,957
241,016
358,913
2,599,887

37,735.042
4,547.474
6,771.949
49,054.465

% Total

kg/yr

kg/batch

100.00
100.00

259,170
259,170

4,890.000
4,890.000

% Total

kg/yr

kg/batch

100.00
100.00

23,638
23,638

446.000
446.000

% Total

kg/yr

kg/batch

100.00
100.00

1,208,400
1,208,400

22,800.000
22,800.000

% Total

kg/yr

kg/batch

100.00
100.00

24,910
24,910

470.000
470.000

kg/kg MP

kg/kg MP

kg/kg MP

kg/kg MP

kg/kg MP

2.5 BULK MATERIALS: SECTION TOTALS (kg/batch)

Raw Material
Water
Glucose
Sodium Chloride
Air
Ammonia
TOTAL

Main Section
49,054.465
4,890.000
446.000
22,800.000
470.000
77,660.465

- Page 3 -

2.6 BULK MATERIALS: SECTION TOTALS (kg/yr)

Raw Material
Water
Glucose
Sodium Chloride
Air
Ammonia
TOTAL

Main Section
2,599,887
259,170
23,638
1,208,400
24,910
4,116,005

- Page 4 -

3. STREAM DETAILS
Stream Name
Source
Destination
Stream Properties

Air Compressed Air

INPUT
P-3
P-3
P-4

Ammonia
INPUT
P-4

Gas to Filter
P-4
P-5

Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

0.00
25.00
1.00
1.16
160.31
6.05
0.24

0.00
40.00
6.00
6.65
256.55
9.68
0.24

0.00
25.00
6.00
4.12
176.17
322.51
0.50

0.00
39.38
6.00
6.57
432.72
16.00
0.25

0.000
17,490.288
5,309.712
22,800.000
19,590,974.088

0.000
17,490.288
5,309.712
22,800.000
3,429,433.471

470.000
0.000
0.000
470.000
114,024.372

470.000
17,490.288
5,309.712
23,270.000
3,542,194.437

Component Flowrates (kg/batch)

Ammonia
Nitrogen
Oxygen
TOTAL (kg/batch)
TOTAL (L/batch)

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Filter to
Fermenter
P-5
P-6

Salts

Glucose

Water

INPUT
P-1

INPUT
P-1

INPUT
P-1

0.00
39.38
6.00
6.57
432.72
16.00
0.25

0.00
25.00
1.01
2,164.00
3.99
7.69
0.31

0.00
25.00
1.01
1,562.00
41.47
7.30
0.29

0.00
25.00
1.01
994.70
971.04
24.96
1.00

470.000
0.000
17,490.288
5,309.712
0.000
0.000
23,270.000
3,542,207.905

0.000
0.000
0.000
0.000
446.000
0.000
446.000
206.100

0.000
4,890.000
0.000
0.000
0.000
0.000
4,890.000
3,130.602

0.000
0.000
0.000
0.000
0.000
33,480.000
33,480.000
33,658.243

Component Flowrates (kg/batch)

Ammonia
Glucose
Nitrogen
Oxygen
Sodium Chloride
Water
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 5 -

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

P-1
P-2

Sterilizer to
Fermenter
P-2
P-6

P-6
OUTPUT

Biomass to
Cooler
P-6
P-7

0.00
25.09
10.12
1,049.19
1,020.15
22.61
0.90

0.00
37.00
10.12
1,045.03
1,504.41
33.35
0.90

0.00
20.00
1.01
1.23
397.85
14.19
0.24

0.00
37.00
1.01
1,009.82
1,579.13
35.75
0.97

0.000
0.000
0.000
4,890.000
0.000
0.000
446.000
33,480.000
38,816.000
36,996.082

0.000
0.000
0.000
4,890.000
0.000
0.000
446.000
33,480.000
38,816.000
37,143.603

232.742
0.000
2,335.901
0.000
17,525.124
4,033.850
0.000
0.000
24,127.616
19,686,658.511

0.000
1,129.134
0.000
1,502.598
0.000
0.000
220.173
35,151.118
38,003.023
37,633.439

To sterilizer

Off gas

Component Flowrates (kg/batch)

Ammonia
Biomass
Carb. Dioxide
Glucose
Nitrogen
Oxygen
Sodium Chloride
Water
TOTAL (kg/batch)
TOTAL (L/batch)

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

S-110
P-7
P-8
0.00
10.00
1.01
1,019.28
426.80
9.66
0.97

Component Flowrates (kg/batch)

Biomass
Glucose
Sodium Chloride
Water
TOTAL (kg/batch)
TOTAL (L/batch)

1,129.134
1,502.598
220.173
35,151.118
38,003.023
37,284.159

- Page 6 -

4. OVERALL COMPONENT BALANCE (kg/batch)

COMPONENT

INITIAL

INPUT

OUTPUT

FINAL

IN-OUT

Ammonia
Biomass
Carb. Dioxide
Glucose
Nitrogen
Oxygen
Sodium Chloride
Water
TOTAL

0.000
0.000
0.000
0.000
120.051
36.445
0.000
0.000
156.496

470.000
0.000
0.000
4,890.000
17,490.288
5,309.712
446.000
49,054.465
77,660.465

232.742
0.000
2,335.901
0.000
17,525.124
4,033.850
0.000
15,574.465
39,702.081

0.140
1,129.134
1.407
1,502.598
85.215
25.095
220.173
35,151.118
38,114.880

237.118
- 1,129.134
- 2,337.308
3,387.402
0.000
1,287.213
225.827
- 1,671.118
0.000

- Page 7 -

5. EQUIPMENT CONTENTS
BT-101
Procedure

Operation

P-1
P-1
P-1
P-1
P-1
P-1
P-1
P-1

START
CHARGE-1 (Charge)
CHARGE-2 (Charge)
CHARGE-3 (Charge)
AGITATE-1 (Agitation)
TRANSFER-OUT-1 (Transfer Out)
CIP-1 (In-Place-Cleaning)
SIP-1 (In-Place-Steaming)

FR-101
Procedure

Operation

P-6
P-6
P-6
P-6
P-6
P-6

START
PULL-IN-1 (Pull In)
FERMENT-1 (Batch Stoich. Fermentation)
TRANSFER-OUT-1 (Transfer Out)
CIP-1 (In-Place-Cleaning)
SIP-1 (In-Place-Steaming)

V-101
Procedure

Operation

P-8
P-8
P-8
P-8

START
TRANSFER-IN-1 (Transfer In)
CIP-1 (In-Place-Cleaning)
SIP-1 (In-Place-Steaming)

Time (in h)

Volume (in L)

0.000
0.833
2.500
3.493
4.493
7.493
7.743
8.243

0.000
33,658.243
36,788.845
36,994.945
36,996.066
0.000
0.000
0.000

Time (in h)

Volume (in L)

0.000
64.943
82.943
146.532
146.782
147.282

0.000
37,143.582
37,633.417
0.000
0.000
0.000

Time (in h)

Volume (in L)

0.000
31.919
32.169
32.669

0.000
37,284.219
37,284.219
37,284.219

- Page 8 -