Forensic Science International: Genetics Supplement Series Manuscript number FSIGSS-D-09-00005

ISSN: 18751768.

DATABASE OF THE POLYMORPHIC GENETIC MARKERS D19S433 AND

D2S1338 FROM THE POPULATION OF BUENOS AIRES PROVINCE,
ARGENTINA.

Celia Iudica, Stella Maris Jaureguiberry, Mara Laura Parolin, Lorena Sambuco.
Laboratorio de Gentica Forense, Superintendencia de Polica Cientfica. Buenos Aires,
Argentina.

Abstract
Short tandem repeat (STR) loci are the most informative DNA genetic markers for
attempting to individualize biological material for application at paternity and forensic
cases. Before a new marker system can be introduced into forensic casework, a database
for the relevant population must be established for statistical evaluation of the evidence.
Therefore, this report presents allele frequency data and parameters of biological or
legal interest, such as heterozygosity value and power of exclusion in Buenos Aires
Province (Argentina) population sample for the loci D19S433 and D2S1338. Blood
samples (N= 473) were collected form individuals unrelated throughout Buenos Aires
province on FTA cards. Following DNA extractions, multiplex PCR amplifications
were carried out

using AmpFlSTR Identifiler PCR Amplification kit

(Applied

Biosystems) and amplified products analized on an Applied Biosystems DNA sequencer

(Model 3130). Heterozygosity value is estimated as 0.81184 for D19S433, and 0.85201
for D2S1338. Both STR loci are in Hardy-Weinberg equilibrium. For D19S433, power
of exclusion and power of discrimination are estimated as 0.621 and 0.937. For
D2S1338 the same parameters are 0.699 and 0.968. The allele frequency data can be
used for deriving estimates of multiple locus profile frequencies for identity testing
purposes.
Keywords: database, Short Tandem Repeat, D19S433, D2S1338, Buenos Aires,
Argentina.
Introduction

Short tandem repeat (STR) loci are the most informative DNA genetic markers for
attempting to individualize biological material for application at paternity and forensic
cases. Before a new marker system can be introduced into forensic casework, a database
for the relevant population must be established for statistical evaluation of the evidence.
Therefore, this report presents allele frequency data and parameters of biological or
legal interest, such as heterozygosity value and power of exclusion in Buenos Aires
Province (Argentina) population sample for the loci D19S433 and D2S1338.

Materials and Methods

Blood samples (N = 473) were collected from unrelated, randomly selected individuals
throughout Buenos Aires province on FTA classic cards. DNA was extracted as
described by the manufacturer (Whatman bioscience, MA, USA) [1]. Multiplex PCR
amplifications were carried out using AmpFlSTR Identifiler PCR Amplification kit
(Applied Biosystems, Foster City, USA) following manufacturer`s instructions [2].
Analysis of the PCR products was performed with the help of an Applied Biosystems
DNA sequencer (Model 3130).
The frequency of each allele for each locus tested was calculated using the number of
observed genotypes in the sample by PowerStats software ver. 1.2 (Promega Corp) [3].
Forensic and paternity parameters were performed using the same software.
The exact test for the Hardy-Weinberg equilibrium and the comparisons between pairs of
populations were carried out using ARLEQUIN software version 3.1 [4].

Results: Allele frequencies for both loci and statistical parameters are shown in Table1.
Analysis across the database reveled 14 D19S433 alleles and 12 D2S1338 alleles. Both
STR loci are in Hardy-Weinberg equilibrium. Heterozygosity value is estimated as
0.81184 for D19S433, and 0.85201 for D2S1338. For D19S433, power of exclusion and
power of discrimination are estimated as 0.621 and 0.937. For D2S1338 the same
parameters are 0.699 and 0.968. Typical Paternity Index is estimated as 2.66 for
D19S433 and 3.38 for D2S1338.
There were no significant differences (P >0.05) between our population data and those
obtained from the province of Cordoba, Argentina [5].
The allele frequency data can be used for deriving estimates of multiple locus profile
frequencies for identity testing purposes.

References:
[1] Whatman FTA from bood DNA. Technical protocol to collect, transport, store and
purify blood DNA: http://www.whatman.com/References/WGP_1351_Blood_B.pdf.
Whatman bioscience, MA, USA.
[2] AmpFlSTR Identifiler PCR Amplification kit. Users manual. Applied Biosystems.
[3] PowerStats V1.2, Promega Corp. Free program distributed by Promega Corp. over
the internet from: http://www.promega.com/geneticidtools/powerstats/Default.htm.
[4] L. Excoffier, G. Laval and S. Schneider. ARLEQUIN, Version 3.1: Software for
population genetics data analysis. Genetics and Biometry Laboratory. Department of
Anthropology, University of Berne. September, 2006. Free program distributed by the
authors over the internet from: http://cmpg.unibe.ch/software/arlequin3.
[5] Agencia Crdoba Ciencia - CEPROCOR STRs data for the Loci D3S1385, vWA,
FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, THO1,
TPOX, CSF1PO, FABP, D6S366, FESFPS, F13A01, Penta E, Penta D, D2S1338 and
D19S433 from Crdoba (Argentina). Recovery at 3 July, 2009 from
http://www.mincyt.cba.gov.ar/multimedia/strs_data.pdf.

Table 1: Allele frequencies and statistical parameters for D19S433 and D2S1338 loci
from Buenos Aires Province, Argentina (N = 473).
Allele
9
10
11
11.2
12
12.2
13
13.2
14
14.2
15
15.2
16
16.2
17
17.2
18
18.2
19
20
21
22
23

D19S433
0.0074
0.0011
0.0751
0.0106
0.2040
0.0518
0.3266
0.0370
0.1670
0.0666
0.0285
0.0222
0.0011
0.0011
-

D2S1338
0.0233
0.2156
0.1004
0.1734
0.1321
0.0349
0.0518
0.1226

24
25
26
27
28
Hob
Hex
PD
PE
1-PE
TPI
PIC
MP
P

0.0772
0.0560
0.0116
0.0011
-

0.812
0.809
0.937
0.621
0.379
2.660
0.790
15.90
0.566

0.852
0.868
0.968
0.699
0.301
3.380
0.850
30.90
0.327

Hob: observed heterozigocity; Hex: expected heterozigocity; PD: power of discrimination; PE:
probability of exclusion; TPI: Typical Paternity Index; MP: matching probability; P: Hardy-Weinberg
equilibrium, exact test based on 10.000 permutations.