Escolar Documentos
Profissional Documentos
Cultura Documentos
February 6, 2014
Name: __________________________________________ eID ________________
Helpful Equations:
degrees K = 273 + C
D) quaternary
E) none of these choices
D) Cys
E) Gln
D) Gly
Page 1
C) H2 O
E) Cys
B.
C.
A) A
B) B
C) C
D.
D) D
E) all of the above
11. Which of the following would be a proper designation of the deoxy position on DNA?
A) 3' (3 prime)
B) 3
C) 2' (2 prime)
D) 2
E) 1
12. If the following two reactions were coupled, what would be the G for the overall exergonic reaction?
ATP + H 2O ADP + P i
G = -31 kJ/mol
Glucose + Pi glucose-1-phosphate + H2O
A) -52 kJ/mol
B) -10 kJ/mol
C) 10 kJ/mol
G = 21 kJ/mol
D) 52 kJ/mol
E) none of the above
13. The most important buffering system for maintaining proper blood pH is:
A) the charges on the amino acids
B) the bicarbonate buffer system of CO2 , carbonic acid, and bicarbonate
C) phosphate groups of serum phosphoproteins
D) none of the above
E) all of the above
14. A protein that consists of a single polypeptide chain will lack which level of protein structure?
A) Primary
B) Secondary
C) Tertiary
D) Quaternary
E) Pentanary
Scrambled version A
Page 2
15. What level of protein structure is characterized by the three dimensional structure of an entire polypeptide
including all amino acid side chains?
A) primary
D) quaternary
B) secondary
E) none of these choices
C) tertiary
16. The Bohr effect describes a lowered O2 -affinity of Hb when the pH _____.
A) increases
B) decreases
17. The higher the dielectric constant, the more ________ the compound.
A) polar
D) nonpolar
B) negatively charged
E) oxidized
C) positively charged
18. Which RNA molecules are involved in the process of translation?
A) mRNA
B) tRNA
C) rRNA
D) A and C
19. Which of the following is a simple statement of Chargaffs rules?
A) A+G = C+T
B) A = C
C) C = T
20. Heme is considered a _____.
A) catalyst
B) prosthetic group
C) coenzyme
E) A, B and C
D) G = A
D) cofactor
E) none of the above
21. Which of the following is the most abundant structural element in the human body?
A) nitrogen
D) phosphorous
B) carbon
E) none of the above
C) oxygen
22. What bonding interactions are broken during DNA denaturation?
A) Covalent bonds
D) B and C
B) Hydrogen bonds
E) A, B and C
C) van der Waals interactions
23. The tertiary structures of myglobin, -hemoglobin and -hemoglobin are nearly identical and are therefore
nearly identical in primary structure.
A) True
B) False
24. Which of the following assembles by association of multiple protofilaments?
A) myosin
B) actin
C) tubulin
D) keratin
Scrambled version A
Page 3
E) collagen
25. What term describes the process of converting the information found in DNA into the sequence of a
protein?
A) replication
D) expression
B) transcription
E) none of the above
C) translation
26. Which of the two proteins below has a longer neck region?
A) myosin
B) keratin
C) kinesin
D) collagen
E) actin
Page 4
E) Met
34. Would DNA-binding proteins be more likely to associate with the major or the minor groove?
A) Major groove
B) Minor groove
35. Identify the amino acid structure below.
A) H
B) I
C) R
D) D
E) V
36. At pH ___, the pentapeptide Ala-Glu-His-Val-Cys would contain two positively charged groups and one
negatively charged group.
A) 1
B) 3.5
C) 5
D) 7
E) 9
37. If a phosphate buffer (pK 6.82) was formulated such that its pH was 7.3, it would be best suited to buffer
against _____. If instead, it was formulated such that its pH was 6.3, it would be best suited to buffer
against _____.
A) acid; base
B) acid; acid
C) base; acid
D) base; base
E) a buffer with a pH that far from the pK would not be an effective buffer
38. Which of the biopolymers is correctly paired with its major function?
A) protein: information encoding
D) polysaccharide: energy storage
B) nucleic acids: energy storage
E) none of the above
C) lipids: information encoding
39. Each kinesin head contains a binding site for _____ and a binding site for _____.
A) tubulin; GTP
D) actin; ATP
B) another kinesin molecule; GTP
E) tubulin; ATP
C) myosin; ATP
40. Which allosteric effector has the greatest ability to stabilize the deoxy state of hemoglobin?
A) BPG
D) H+
B) CO2
E) all of the above have equal ability
C) O2
Scrambled version A
Page 5
41. A plot of the binding of oxygen to myoglobin as a function of pO2 gives a _____ shape; a similar plot for
hemoglobin gives a _____ shape.
A) sigmoidal; sigmoidal
D) hyperbolic; hyperbolic
B) sigmoidal; hyperbolic
E) hyperbolic; exponential
C) hyperbolic; sigmoidal
42. If the following mixture of proteins was applied to a size-exclusion chromatography column, what would
be the order of elution?
Proteins with molecular weights (kDa = kiloDalton = kg/mol): myoglobin (17.7 kDa) , hemoglobin (64.5
kDa) , lysozyme (14.3 kDa) and triose phosphate isomerase (57.4 kDa)
A)
B)
C)
D)
E)
43. You are involved in the purification of Protein X with a pI of 6.0 using a buffer with a pH of 7.5. What
type of resin would you choose to ensure initial binding of Protein X?
A) DEAE (pK ~10)
B) CM (pK ~4)
44. Which of the following processes are involved in gene expression?
A) Replication
B) Transcription
C) Translation
D) Both B and C
E) A, B and C
45. The pK of CH3 CH2 NH3 + is 10.7. Would the pK of FCH2 CH2 NH3 + be higher or lower?
A) Higher
B) Lower
46. Which of the following methods of amino acid sequence determination requires an unmodified amino
terminus?
A) Mass spectroscopy
D) Edman sequencing
B) Ion exchange
E) X-ray crystallography
C) SDS-PAGE
47. Which of the following is an example of a conservative amino acid substitution?
A) Ile Leu
B) Ser Glu
C) Phe Tyr
D) Asp Gln
E) Arg Cys
48. What is the fractional saturation (p5 0 = 2.8 torr) of myoglobin when pO2 = 15 torr?
A) 0.65
D) 1.23
B) 0.58
E) None of the above are correct
C) 0.84
Scrambled version A
Page 6
49. What would be the resulting pH if one drop (0.05 ml) of 1.0 M HCl was added to one liter of pure water
(assume pH 7.0)?
A) 2.7
D) 7.0 (there would be no significant change)
B) 4.3
E) 9.7
C) 5.0
50. What is the net charge of the heptapeptide His-Ala-Cys-Asp-Ser-Glu-Asn at pH 7?
A) -2
D) +1
B) -3
E) none of these choices
C) 0
51. Do Chargaffs rules apply to RNA as well as to DNA?
A) Yes
B) No
52. For a globular protein that is found in the cytosol, ___ would most likely be found in the proteins interior
while ___ would most likely be found on the surface.
A) Val; Phe
D) Thr; His
B) Leu; Ile
E) none of these choices
C) Leu; Ser
53. Which of the following would represent an increase in entropy?
A) Ice freezes
D) Sewing a garment
B) Traffic congestion on highway
E) Filing papers
C) Dismissal of class when bell rings
54. Which of the following atoms would be considered a hydrogen bond donor in an -helix?
A) a
B) b
C) c
D) d
E) none of these choices
55. What is the pH of a solution that contains one part acetic acid and three parts sodium acetate? The pK for
acetic acid is 4.76.
A) 5.24
B) 5.06
C) 4.46
D) 4.28
E) cannot be determined from the given information
Scrambled version A
Page 7
56. Which of the following fibers is correctly paired with the protein that forms the fiber?
A) microfilaments: actin
D) intermediate filaments: keratin
B) microtubule: tubulin
E) all of the above
C) extracellular support fibers: collagen
57. Which of the following explains the attractive forces between hydrophobic molecules in an aqueous
solution?
A) in an aqueous environment, London dispersion forces between hydrophobic molecules become
stronger
B) in an aqueous environment, London dispersion forces between hydrophobic molecules and water
become stronger
C) since nonpolar molecules do not form hydrogen bonds with hydrogen bonds with water, they can form
hydrogen bonds with other nonpolar molecules
D) there is no increase in attractive forces between nonpolar molecules in an aqueous environment
E) none of the above
A) (i) Hb A; (ii) Hb A
B) (i) Hb A; (ii) Hb Great Lakes
C) (i) Hb Great Lakes; (ii) Hb A
Scrambled version A
Page 8
59. Biochemists sometimes link a recombinant (modified) protein to green fluorescent protein (GFP). The
fluorophore in GFP is a derivative of three consecutive amino acids that undergo cyclization of the
polypeptide chain and an oxidation. Using the structure below, identify: (i) the three amino acid residues;
and (ii) the bond that results from oxidation.
A) Met-Tyr-Ala; c
B) Ser-Tyr-Gly; a
C) Ser-Phe-Gly; c
D) Ser-Tyr-Gly; b
E) Met-Tyr-Ala; a
60. Based on the following Tm values, what can be said about these two DNA molecules: Strand A = 80C;
Strand B = 100C?
A) Strand A has a higher percentage of adenine bases.
B) Strand B has a higher percentage of cytosine bases.
C) Strand B has stronger stacking interactions.
D) All of the above
61. Rank the solubilities of the following amino acids in water at pH 7: W, R, S, V, T.
A) V < W < T < S < R
D) W < V < T < S < R
B) W < V < S < T < R
E) R < T < S < V < W
C) V < W < S < T < R
62. Urea (NH2 CONH2 ) dissolves readily in water. The beaker containing the dissolved compound is cold to
the touch. What conclusions can you make about the sign of the (i) enthalpy change; and (ii) entropy
change for this process?
A) + H; + S
D) - H; + S
B) - H; - S
E) Cannot be determined
C) + H; - S
63. A solution is made by mixing 25 mL of a stock solution of 2.0 M K2 HPO4 (pK 6.82) and 50 mL of a stock
solution of 2.0 M KH2 PO4 . The solution is diluted to a final volume of 200 mL. What is the final pH of
the solution?
A) 7.12
B) 6.82
C) 6.52
D) 6.22
E) 7.42
Scrambled version A
Page 9
A
B
A
B
D
D
D
B
C
A
C
B
B
D
C
B
A
E
A
B
B
D
B
C
D
A
E
C
C
C
E
B
C
A
B
B
A
D
E
A
C
D
A
D
Scrambled version A
Page 10
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
57.
58.
59.
60.
61.
62.
63.
B
D
A
C
B
A
A B
C
C
A
A
E
D
C
B
D
D
A
C
Scrambled version A
Page 11
CH 369 Exam 1
February 6, 2014
Name: __________________________________________ eID ________________
Helpful Equations:
degrees K = 273 + C
D) quaternary
E) none of these choices
E) collagen
4. Which of the following is the most abundant structural element in the human body?
A) nitrogen
D) phosphorous
B) carbon
E) none of the above
C) oxygen
5. Which of the 20 standard amino acids is optically inactive?
A) Arg
B) Pro
C) Ile
D) Gly
6. In a muscle cell, what is the name for the bundles of myosin tails?
A) cytoskeleton
D) thin filaments
B) thick filaments
E) none of the above
C) intermediate filaments
Scrambled version B
Page 1
E) Cys
A) A
B) B
C) C
B.
C.
D.
D) D
E) all of the above
E) Met
12. The most important buffering system for maintaining proper blood pH is:
A) the charges on the amino acids
B) the bicarbonate buffer system of CO2 , carbonic acid, and bicarbonate
C) phosphate groups of serum phosphoproteins
D) none of the above
E) all of the above
13. A protein that consists of a single polypeptide chain will lack which level of protein structure?
A) Primary
B) Secondary
C) Tertiary
D) Quaternary
E) Pentanary
Scrambled version B
Page 2
14. Which of the following would have the lowest melting point?
A) NH3
B) CH4
C) H2 O
15. The tertiary structures of myglobin, -hemoglobin and -hemoglobin are nearly identical and are therefore
nearly identical in primary structure.
A) True
B) False
16. Heme is considered a _____.
A) catalyst
B) prosthetic group
C) coenzyme
D) cofactor
E) none of the above
D) Cys
E) Gln
18. Which of the following amino acids has a nonpolar side chain?
A) Ser
D) Asn
B) Val
E) none of these choices
C) Lys
19. Which of the two proteins below has a longer neck region?
A) myosin
B) keratin
C) kinesin
D) collagen
E) actin
Scrambled version B
Page 3
23. If the following two reactions were coupled, what would be the G for the overall exergonic reaction?
ATP + H 2O ADP + P i
G = -31 kJ/mol
Glucose + Pi glucose-1-phosphate + H2O
A) -52 kJ/mol
B) -10 kJ/mol
C) 10 kJ/mol
G = 21 kJ/mol
D) 52 kJ/mol
E) none of the above
24. The Bohr effect describes a lowered O2 -affinity of Hb when the pH _____.
A) increases
B) decreases
25. Which of the following would be a proper designation of the deoxy position on DNA?
A) 3' (3 prime)
B) 3
C) 2' (2 prime)
D) 2
E) 1
26. What term describes the process of converting the information found in DNA into the sequence of a
protein?
A) replication
D) expression
B) transcription
E) none of the above
C) translation
27. What is the conjugate acid of H2 PO4 -?
A) HPO4 2B) H2 PO4
C) H3 PO4
28. Which of the following molecules contains the most oxidized form of carbon?
A) acetaldehyde
D) ethylene
B) ethanol
E) carbon dioxide
C) acetic acid
29. What bonding interactions are broken during DNA denaturation?
A) Covalent bonds
D) B and C
B) Hydrogen bonds
E) A, B and C
C) van der Waals interactions
30. Which RNA molecules are involved in the process of translation?
A) mRNA
B) tRNA
C) rRNA
D) A and C
31. The higher the dielectric constant, the more ________ the compound.
A) polar
D) nonpolar
B) negatively charged
E) oxidized
C) positively charged
Scrambled version B
Page 4
E) A, B and C
D) G = A
36. Which of the following methods of amino acid sequence determination requires an unmodified amino
terminus?
A) Mass spectroscopy
D) Edman sequencing
B) Ion exchange
E) X-ray crystallography
C) SDS-PAGE
37. If a phosphate buffer (pK 6.82) was formulated such that its pH was 7.3, it would be best suited to buffer
against _____. If instead, it was formulated such that its pH was 6.3, it would be best suited to buffer
against _____.
A) acid; base
B) acid; acid
C) base; acid
D) base; base
E) a buffer with a pH that far from the pK would not be an effective buffer
38. Do Chargaffs rules apply to RNA as well as to DNA?
A) Yes
B) No
39. Which of the following processes are involved in gene expression?
A) Replication
B) Transcription
C) Translation
D) Both B and C
Scrambled version B
Page 5
E) A, B and C
40. At pH ___, the pentapeptide Ala-Glu-His-Val-Cys would contain two positively charged groups and one
negatively charged group.
A) 1
B) 3.5
C) 5
D) 7
E) 9
41. Each kinesin head contains a binding site for _____ and a binding site for _____.
A) tubulin; GTP
D) actin; ATP
B) another kinesin molecule; GTP
E) tubulin; ATP
C) myosin; ATP
42. What is the net charge of the heptapeptide His-Ala-Cys-Asp-Ser-Glu-Asn at pH 7?
A) -2
D) +1
B) -3
E) none of these choices
C) 0
43. Which of the following fibers is correctly paired with the protein that forms the fiber?
A) microfilaments: actin
D) intermediate filaments: keratin
B) microtubule: tubulin
E) all of the above
C) extracellular support fibers: collagen
44. What would be the resulting pH if one drop (0.05 ml) of 1.0 M HCl was added to one liter of pure water
(assume pH 7.0)?
A) 2.7
D) 7.0 (there would be no significant change)
B) 4.3
E) 9.7
C) 5.0
45. What is the fractional saturation (p5 0 = 2.8 torr) of myoglobin when pO2 = 15 torr?
A) 0.65
D) 1.23
B) 0.58
E) None of the above are correct
C) 0.84
46. You are involved in the purification of Protein X with a pI of 6.0 using a buffer with a pH of 7.5. What
type of resin would you choose to ensure initial binding of Protein X?
A) DEAE (pK ~10)
B) CM (pK ~4)
47. Which of the following would represent an increase in entropy?
A) Ice freezes
D) Sewing a garment
B) Traffic congestion on highway
E) Filing papers
C) Dismissal of class when bell rings
48. Which of the following statements regarding RNA is FALSE?
A) The ribose in RNA contains an O atom that the ribose in DNA lacks.
B) RNA does not form a 2-stranded double helix like DNA.
C) Since RNA does not form a double helix, it tends to be linear in shape.
D) RNA can form Watson-Crick base pairs with DNA.
Scrambled version B
Page 6
49. Which of the following explains the attractive forces between hydrophobic molecules in an aqueous
solution?
A) in an aqueous environment, London dispersion forces between hydrophobic molecules become
stronger
B) in an aqueous environment, London dispersion forces between hydrophobic molecules and water
become stronger
C) since nonpolar molecules do not form hydrogen bonds with hydrogen bonds with water, they can form
hydrogen bonds with other nonpolar molecules
D) there is no increase in attractive forces between nonpolar molecules in an aqueous environment
E) none of the above
50. What is the pH of a solution that contains one part acetic acid and three parts sodium acetate? The pK for
acetic acid is 4.76.
A) 5.24
B) 5.06
C) 4.46
D) 4.28
E) cannot be determined from the given information
51. Identify the amino acid structure below.
A) H
B) I
C) R
D) D
E) V
E) Arg Cys
53. For a globular protein that is found in the cytosol, ___ would most likely be found in the proteins interior
while ___ would most likely be found on the surface.
A) Val; Phe
D) Thr; His
B) Leu; Ile
E) none of these choices
C) Leu; Ser
Scrambled version B
Page 7
54. Which of the following atoms would be considered a hydrogen bond donor in an -helix?
A) a
B) b
C) c
D) d
E) none of these choices
55. Which of the biopolymers is correctly paired with its major function?
A) protein: information encoding
D) polysaccharide: energy storage
B) nucleic acids: energy storage
E) none of the above
C) lipids: information encoding
56. Which allosteric effector has the greatest ability to stabilize the deoxy state of hemoglobin?
A) BPG
D) H+
B) CO2
E) all of the above have equal ability
C) O2
57. The pK of CH3 CH2 NH3 + is 10.7. Would the pK of FCH2 CH2 NH3 + be higher or lower?
A) Higher
B) Lower
Page 8
61. The oxygen-binding curves for normal hemaglobin (Hb A) and a mutant hemoglobin (Hb Great Lakes) are
shown in the figure below. Which of the two hemoglobins: (i) has a higher affinity for oxygen when pO2 =
20 torr; and (ii) is most efficient at delivering oxygen from arterial blood (pO2 = 75 torr) to active muscle
(pO2 = 20 torr)?
A) (i) Hb A; (ii) Hb A
B) (i) Hb A; (ii) Hb Great Lakes
C) (i) Hb Great Lakes; (ii) Hb A
62. A solution is made by mixing 25 mL of a stock solution of 2.0 M K2 HPO4 (pK 6.82) and 50 mL of a stock
solution of 2.0 M KH2 PO4 . The solution is diluted to a final volume of 200 mL. What is the final pH of
the solution?
A) 7.12
B) 6.82
C) 6.52
D) 6.22
E) 7.42
63. Biochemists sometimes link a recombinant (modified) protein to green fluorescent protein (GFP). The
fluorophore in GFP is a derivative of three consecutive amino acids that undergo cyclization of the
polypeptide chain and an oxidation. Using the structure below, identify: (i) the three amino acid residues;
and (ii) the bond that results from oxidation.
A) Met-Tyr-Ala; c
B) Ser-Tyr-Gly; a
C) Ser-Phe-Gly; c
D) Ser-Tyr-Gly; b
E) Met-Tyr-Ala; a
Scrambled version B
Page 9
C
B
C
B
D
B
A
A
C
C
C
B
D
B
B
B
D
B
A
E
D
A
B
B
C
D
C
E
D
E
A
A
A
C
D
D
A
A B
D
B
E
A
E
B
Scrambled version B
Page 10
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
57.
58.
59.
60.
61.
62.
63.
C
A
C
C
D
A
B
A
C
A
D
A
B
D
A
D
C
C
B
Scrambled version B
Page 11
CH 369 Exam 1
February 6, 2014
Name: __________________________________________ eID ________________
Helpful Equations:
degrees K = 273 + C
D) Gly
E) Cys
5. What level of protein structure is characterized by the three dimensional structure of an entire polypeptide
including all amino acid side chains?
A) primary
D) quaternary
B) secondary
E) none of these choices
C) tertiary
6. The tertiary structures of myglobin, -hemoglobin and -hemoglobin are nearly identical and are therefore
nearly identical in primary structure.
A) True
B) False
7. What bonding interactions are broken during DNA denaturation?
A) Covalent bonds
D) B and C
B) Hydrogen bonds
E) A, B and C
C) van der Waals interactions
8. Which of the following is a simple statement of Chargaffs rules?
A) A+G = C+T
B) A = C
C) C = T
D) G = A
9. Which of the following would be a proper designation of the deoxy position on DNA?
A) 3' (3 prime)
B) 3
C) 2' (2 prime)
D) 2
E) 1
Scrambled version C
Page 1
12. Which of the following amino acids has a nonpolar side chain?
A) Ser
D) Asn
B) Val
E) none of these choices
C) Lys
13. Which of the following would have the lowest melting point?
A) NH3
B) CH4
14. Which amino acid is critical for crosslinking of keratin fibers?
A) Ser
B) Lys
C) His
C) H2 O
D) Cys
E) Gln
15. The higher the dielectric constant, the more ________ the compound.
A) polar
D) nonpolar
B) negatively charged
E) oxidized
C) positively charged
16. What term describes the process of converting the information found in DNA into the sequence of a
protein?
A) replication
D) expression
B) transcription
E) none of the above
C) translation
17. -helices and -sheets comprise ___ structure.
A) primary
B) secondary
C) tertiary
D) quaternary
E) none of these choices
18. Which of the two proteins below has a longer neck region?
A) myosin
B) keratin
C) kinesin
D) collagen
Page 2
E) actin
E) A, B and C
Scrambled version C
Page 3
E) Met
B.
C.
A) A
B) B
C) C
D.
D) D
E) all of the above
D) cofactor
E) none of the above
30. Which of the following is the most abundant structural element in the human body?
A) nitrogen
D) phosphorous
B) carbon
E) none of the above
C) oxygen
31. Which of the following assembles by association of multiple protofilaments?
A) myosin
B) actin
C) tubulin
D) keratin
E) collagen
32. If the following two reactions were coupled, what would be the G for the overall exergonic reaction?
ATP + H 2O ADP + P i
G = -31 kJ/mol
Glucose + Pi glucose-1-phosphate + H2O
A) -52 kJ/mol
B) -10 kJ/mol
C) 10 kJ/mol
G = 21 kJ/mol
D) 52 kJ/mol
E) none of the above
Scrambled version C
Page 4
A) a
B) b
C) c
D) d
E) none of these choices
A) H
B) I
C) R
Scrambled version C
D) D
Page 5
E) V
41. Which allosteric effector has the greatest ability to stabilize the deoxy state of hemoglobin?
A) BPG
D) H+
B) CO2
E) all of the above have equal ability
C) O2
42. What is the pH of a solution that contains one part acetic acid and three parts sodium acetate? The pK for
acetic acid is 4.76.
A) 5.24
B) 5.06
C) 4.46
D) 4.28
E) cannot be determined from the given information
43. Would DNA-binding proteins be more likely to associate with the major or the minor groove?
A) Major groove
B) Minor groove
44. What is the fractional saturation (p5 0 = 2.8 torr) of myoglobin when pO2 = 15 torr?
A) 0.65
D) 1.23
B) 0.58
E) None of the above are correct
C) 0.84
45. What would be the resulting pH if one drop (0.05 ml) of 1.0 M HCl was added to one liter of pure water
(assume pH 7.0)?
A) 2.7
D) 7.0 (there would be no significant change)
B) 4.3
E) 9.7
C) 5.0
46. Which of the following would represent an increase in entropy?
A) Ice freezes
D) Sewing a garment
B) Traffic congestion on highway
E) Filing papers
C) Dismissal of class when bell rings
47. Which of the following is an example of a conservative amino acid substitution?
A) Ile Leu
B) Ser Glu
C) Phe Tyr
D) Asp Gln
E) Arg Cys
48. For a globular protein that is found in the cytosol, ___ would most likely be found in the proteins interior
while ___ would most likely be found on the surface.
A) Val; Phe
D) Thr; His
B) Leu; Ile
E) none of these choices
C) Leu; Ser
49. Which of the following processes are involved in gene expression?
A) Replication
B) Transcription
C) Translation
D) Both B and C
Scrambled version C
Page 6
E) A, B and C
50. Which of the following fibers is correctly paired with the protein that forms the fiber?
A) microfilaments: actin
D) intermediate filaments: keratin
B) microtubule: tubulin
E) all of the above
C) extracellular support fibers: collagen
51. If the following mixture of proteins was applied to a size-exclusion chromatography column, what would
be the order of elution?
Proteins with molecular weights (kDa = kiloDalton = kg/mol): myoglobin (17.7 kDa) , hemoglobin (64.5
kDa) , lysozyme (14.3 kDa) and triose phosphate isomerase (57.4 kDa)
A)
B)
C)
D)
E)
52. You are involved in the purification of Protein X with a pI of 6.0 using a buffer with a pH of 7.5. What
type of resin would you choose to ensure initial binding of Protein X?
A) DEAE (pK ~10)
B) CM (pK ~4)
53. Which of the following statements regarding RNA is FALSE?
A) The ribose in RNA contains an O atom that the ribose in DNA lacks.
B) RNA does not form a 2-stranded double helix like DNA.
C) Since RNA does not form a double helix, it tends to be linear in shape.
D) RNA can form Watson-Crick base pairs with DNA.
54. Each kinesin head contains a binding site for _____ and a binding site for _____.
A) tubulin; GTP
D) actin; ATP
B) another kinesin molecule; GTP
E) tubulin; ATP
C) myosin; ATP
55. Which of the following explains the attractive forces between hydrophobic molecules in an aqueous
solution?
A) in an aqueous environment, London dispersion forces between hydrophobic molecules become
stronger
B) in an aqueous environment, London dispersion forces between hydrophobic molecules and water
become stronger
C) since nonpolar molecules do not form hydrogen bonds with hydrogen bonds with water, they can form
hydrogen bonds with other nonpolar molecules
D) there is no increase in attractive forces between nonpolar molecules in an aqueous environment
E) none of the above
Scrambled version C
Page 7
56. A plot of the binding of oxygen to myoglobin as a function of pO2 gives a _____ shape; a similar plot for
hemoglobin gives a _____ shape.
A) sigmoidal; sigmoidal
D) hyperbolic; hyperbolic
B) sigmoidal; hyperbolic
E) hyperbolic; exponential
C) hyperbolic; sigmoidal
57. If a phosphate buffer (pK 6.82) was formulated such that its pH was 7.3, it would be best suited to buffer
against _____. If instead, it was formulated such that its pH was 6.3, it would be best suited to buffer
against _____.
A) acid; base
B) acid; acid
C) base; acid
D) base; base
E) a buffer with a pH that far from the pK would not be an effective buffer
A) (i) Hb A; (ii) Hb A
B) (i) Hb A; (ii) Hb Great Lakes
C) (i) Hb Great Lakes; (ii) Hb A
59. Based on the following Tm values, what can be said about these two DNA molecules: Strand A = 80C;
Strand B = 100C?
A) Strand A has a higher percentage of adenine bases.
B) Strand B has a higher percentage of cytosine bases.
C) Strand B has stronger stacking interactions.
D) All of the above
Scrambled version C
Page 8
A) Met-Tyr-Ala; c
B) Ser-Tyr-Gly; a
C) Ser-Phe-Gly; c
D) Ser-Tyr-Gly; b
E) Met-Tyr-Ala; a
62. A solution is made by mixing 25 mL of a stock solution of 2.0 M K2 HPO4 (pK 6.82) and 50 mL of a stock
solution of 2.0 M KH2 PO4 . The solution is diluted to a final volume of 200 mL. What is the final pH of
the solution?
A) 7.12
B) 6.82
C) 6.52
D) 6.22
E) 7.42
63. Urea (NH2 CONH2 ) dissolves readily in water. The beaker containing the dissolved compound is cold to
the touch. What conclusions can you make about the sign of the (i) enthalpy change; and (ii) entropy
change for this process?
A) + H; + S
D) - H; + S
B) - H; - S
E) Cannot be determined
C) + H; - S
Scrambled version C
Page 9
B
D
B
D
C
B
D
A
C
A
C
B
B
D
A
D
B
A
E
E
D
B
C
A
C
E
C
A
B
B
C
B
A B
B
A
D
D
B
A
B
A
A
A
C
Scrambled version C
Page 10
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
57.
58.
59.
60.
61.
62.
63.
B
C
A
C
D
E
D
A
C
E
D
C
A
C
D
D
B
C
A
Scrambled version C
Page 11