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Ejercicio N 2:
FISIOLOGA DEL MSCULO ESQUELTICO-ACTIVIDADES DEL 1 AL 7.
Exercise 2: Skeletal Muscle Physiology: Activity 1: The Muscle Twitch and the Latent Period Lab Report
Pre-lab Quiz Results
You scored 100% by answering 5 out of 5 questions correctly.
1. Skeletal muscles are connected to bones by
You correctly answered: b. tendons.
2. Skeletal muscles are composed of hundreds to thousands of individual cells called
You correctly answered: c. fibers.
3. The term motor unit refers to
You correctly answered: c. one motor neuron and all of the skeletal muscle fibers it innervates.
4. The motor neuron and muscle fiber intersect at what is called
You correctly answered: d. the neuromuscular junction.
5. A twitch is
You correctly answered: a. one contractile response to a single action potential.
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Experiment Results
Predict Question:
Predict Question: Will changes to the stimulus voltage alter the duration of the latent period?
Your answer : b. No, changing the stimulus voltage will not change the latent period duration.
Stop & Think Questions:
What is the period of time that elapses between the generation of an action potential and the start of muscle tension
development in a muscle fiber?
You correctly answered: c. the latent period
What occurs during the latent period of these isometric contractions?
You correctly answered: b. All the steps of excitation-contraction coupling occur.
Experiment Data:
Voltage
0.0
3.0
4.0
6.0
8.0
10.0
Length
75
75
75
75
75
75
Active Force
0.00
1.04
1.32
1.65
1.81
1.82
Passive Force
0.00
0.00
0.00
0.00
0.00
0.00
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Total Force
0.00
1.04
1.32
1.65
1.81
1.82
Latent Period
----0.40
----------
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Exercise 2: Skeletal Muscle Physiology: Activity 2: The Effect of Stimulus Voltage on Skeletal Muscle Contraction Lab
Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. Skeletal muscle fibers are innervated (stimulated) by
You correctly answered: c. motor neurons.
2. A single action potential propagating down a motor axon results in
You correctly answered: d. a single action potential and a single contractile event in the muscle fibers it innervates.
3. In resting skeletal muscle, calcium is stored in
You correctly answered: c. the sarcoplasmic reticulum.
4. During the latent period for an isometric contraction
You correctly answered: c. the cellular events involved in excitation-contraction coupling occur.
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Experiment Results
Predict Question:
Predict Question: As the stimulus voltage is increased from 1.0 volt up to 10 volts, what will happen to the amount of active
force generated with each stimulus?
Your answer : c. The active force will first increase and then plateau at some maximal value as the stimulus voltage
increases.
Stop & Think Questions:
What do you see in the active force display when the stimulus voltage is set to 0.0, and why does this observation make
sense?
You correctly answered: a. 0.00 g; there was no activation of skeletal muscle fibers by this stimulus.
What is the lowest stimulus voltage that induces active force in the skeletal muscle?
You correctly answered: b. threshold voltage
6. Enter the threshold voltage for this experiment in the field below and then click Submit to record your answer in the lab
report
You answered: 0.8 volts
12. Enter the maximal voltage for this experiment in the field below and then click Submit to record your answer in the lab
report.
You answered: 10 volts
Experiment Data:
Voltage
0.0
0.2
0.8
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
Length
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
75
Active Force
0.00
0.00
0.02
0.15
0.43
0.66
0.87
1.04
1.19
1.32
1.42
1.51
1.59
1.65
1.70
1.74
1.78
1.81
1.82
1.82
1.82
1.82
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Passive Force
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
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Total Force
0.00
0.00
0.02
0.15
0.43
0.66
0.87
1.04
1.19
1.32
1.42
1.51
1.59
1.65
1.70
1.74
1.78
1.81
1.82
1.82
1.82
1.82
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Exercise 2: Skeletal Muscle Physiology: Activity 3: The Effect of Stimulus Frequency on Skeletal Muscle Contraction Lab
Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. During a single twitch of a skeletal muscle
You correctly answered: b. maximal force is never achieved.
2. When a skeletal muscle is repetitively stimulated, twitches can overlap each other and result in a stronger muscle
contraction than a stand-alone twitch. This phenomenon is known as
You correctly answered: c. wave summation.
3. Wave summation is achieved by
You correctly answered: a. increasing the stimulus frequency (the rate of stimulus delivery to the muscle).
4. Wave summation increases the force produced in the muscle. Another way to increase the force produced by a muscle
is to
You correctly answered: d. increase the number of activated motor units.
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Experiment Results
Predict Question:
Predict Question 1: As the stimulus frequency increases, what will happen to the muscle force generated with each
successive stimulus? Will there be a limit to this response?
Your answer : d. As the stimulus frequency increases, the muscle force generated by each successive stimulus will first
increase and then decrease as the stimulus frequency becomes very high.
Predict Question 2: In order to produce sustained muscle contractions with an active force value of 5.2 grams, do you think
you will need to increase the stimulus voltage?
Your answer : a. yes
Stop & Think Questions:
Was there any change in the force generated by the muscle during the second stimulated twitch?
You correctly answered: c. Yes, the second twitch generated more muscle force.
Is the total muscle force generated by the higher frequency stimulation greater than the force generated in previous
stimulations?
You correctly answered: a. Yes, it is greater than the previous stimulations.
Does the force generated by the muscle change with each additional stimulus?
You correctly answered: b. As the stimulus frequency increased, the muscle tension generated by each successive stimulus
also increased, and a limiting maximum value was observed.
Experiment Data:
Voltage
8.5
8.5
8.5
8.5
10
8.5
Length
75
75
75
75
75
75
Stimulus
Single
Multiple
Multiple
Multiple
Multiple
Multiple
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Active Force
1.83
1.83
1.83
4.45
4.64
5.72
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Passive Force
0.00
0.00
0.00
0.00
0.00
0.00
Total Force
1.83
1.83
1.83
4.45
4.64
5.72
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4. To achieve an active force of 5.2 g, did you have to increase the stimulus voltage above 8.5 volts? If not, how did you
achieve an active force of 5.2 g? How well did the results compare with your prediction?
Your answer:
s, se logro aumentar la fuerza del musculo, elevando la frecuencia de las estimulaciones pero usando el voltaje maximo
de 8.5
5. Compare and contrast frequency-dependent wave summation with motor unit recruitment (previously observed by
increasing the stimulus voltage). How are they similar? How was each achieved in the experiment? Explain how each is
achieved in vivo.
Your answer:
la frecuencia depende de la la sumacion de impulsos que se pueda dar, estos generaran una fuerza segun sea la
frecuanecia, pero otra variable a tener en cuenta es la intensidad del voltaje para el efecto escalera, por ejemplo al
aumentar el voltaje a 10, este voltaje no dia oportunidad para que el musculo se relaje y continue eficientemente el efecto,
en cambio con un voltaje de 8.5 (voltaje maximo) si dio oportunidad para que el musculo se relaje y siga con el efecto.
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Exercise 2: Skeletal Muscle Physiology: Activity 4: Tetanus in Isolated Skeletal Muscle Lab Report
Pre-lab Quiz Results
You scored 100% by answering 3 out of 3 questions correctly.
1. Stimulus frequency refers to
You correctly answered: b. the rate that stimulating voltage pulses are applied to an isolated whole skeletal muscle.
2. Which of the following distinguishes a state of unfused tetanus from a state of complete (fused) tetanus?
You correctly answered: d. Muscle tension increases and decreases during a state of unfused tetanus.
3. When the stimulus frequency reaches a value beyond which no further increases in force are generated by the muscle,
the muscle has reached its
You correctly answered: a. maximal tetanic tension.
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Experiment Results
Predict Question:
Predict Question: As the stimulus frequency increases further, what will happen to the muscle tension and twitch
appearance with each successive stimulus? Will there be a limit to this response?
Your answer : b. As the stimulus frequency increases, the muscle tension generated by each successive stimulus will
increase. There will be a limit to this increase.
Stop & Think Questions:
What begins to happen at around 80 msec?
You correctly answered: c. unfused tetanus develops
How does the trace at 130 stimuli/sec compare with the trace at 50 stimuli/sec?
You correctly answered: b. Fused tetanus develops at this greater stimulus frequency.
How do the traces with 146150 stimuli per second compare with the trace at 130 stimuli per second?
You correctly answered: d. Maximal tetanic tension develops with these very high stimulation frequencies.
Experiment Data:
Voltage
8.5
8.5
8.5
8.5
8.5
8.5
8.5
8.5
Length
75
75
75
75
75
75
75
75
Stimuli/sec
50
130
140
142
144
146
148
150
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Active Force
5.12
5.88
5.91
5.94
5.94
5.95
5.95
5.95
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Passive Force
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Total Force
5.12
5.88
5.91
5.94
5.94
5.95
5.95
5.95
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Exercise 2: Skeletal Muscle Physiology: Activity 5: Fatigue in Isolated Skeletal Muscle Lab Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. When skeletal muscle twitches fuse so that the peaks and valleys of each twitch become indistinguishable from each
other, the muscle is in a state known as
You correctly answered: d. complete (fused) tetanus.
2. When the stimulus frequency reaches a value beyond which no further increase of skeletal muscle force can occur, the
muscle has reached its
You correctly answered: c. maximal tetanic tension.
3. A decline in a muscle's ability to maintain a constant level of force, or tension, after prolonged, repetitive stimulation is
called
You correctly answered: c. fatigue.
4. Which of the following is not thought to be a contributing factor to the development of fatigue?
You correctly answered: a. buildup of Ca2+ in the muscle fibers
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Experiment Results
Predict Question:
Predict Question: If the stimulator is briefly turned off for defined periods of time, what will happen to the length of time that
the muscle is able to sustain maximal developed tension when the stimulator is turned on again?
Your answer : b. The length of the rest period will proportionately increase the length of time for sustained muscle tension.
Stop & Think Questions:
Why does the stimulated muscle force begin to decrease over time despite the maintained stimuli? (Note that a decrease in
maximal force indicates muscle fatigue is developing.)
You correctly answered: d. More than one of these answers could be correct.
Why did the length of the intervening rest period affect the length of time the skeletal muscle can maintain maximum tension
once the stimulator is turned on again?
You correctly answered: c. Intracellular concentrations of ADP and Pi declined during the rest period.
Experiment Data:
Voltage
Stimuli/sec
8.5
8.5
8.5
8.5
120
120
120
120
0
0
8
21
5.86
5.86
5.86
5.86
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4. List a few ways that humans could delay the onset of fatigue when they are vigorously using their skeletal muscles.
Your answer:
-Dandole un periodo adecuado de descanso a los musculos.
-Trabajando la respiracin, para poder llevar oxigeno a la sangre.
-adecuarse a entrenamientos si el ejercicio es para periodos cortos o largos.
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Exercise 2: Skeletal Muscle Physiology: Activity 6: The Skeletal Muscle Length-Tension Relationship Lab Report
Pre-lab Quiz Results
You scored 100% by answering 5 out of 5 questions correctly.
1. During an isometric contraction
You correctly answered: d. the skeletal muscle is generating force, but it remains at a fixed length.
2. The force that results from muscles being stretched is
You correctly answered: a. passive force.
3. Active force
You correctly answered: b. is determined by the amount of myosin bound to actin.
4. When you generate the isometric length-tension curve, which of the following forces will not be indicated on your
screen?
You correctly answered: c. tetanic force
5. Passive force in skeletal muscle is largely caused by
You correctly answered: c. the protein titin.
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Experiment Results
Predict Question:
Predict Question: As the resting length of the muscle is changed, what will happen to the amount of total force the muscle
generates during the stimulated twitch?
Your answer : c. Total force can increase or decrease depending upon the starting resting length.
Stop & Think Questions:
Note the dip in total force at a muscle length of 90 mm. Why does this occur?
You correctly answered: d. At this muscle length, active force has decreased in value and passive force has not yet
increased to a significant value.
Experiment Data:
Voltage
8.5
8.5
8.5
8.5
8.5
8.5
8.5
8.5
8.5
Length
75
70
65
60
55
50
80
90
100
Active Force
1.82
1.75
1.55
1.21
0.73
0.11
1.75
1.21
0.11
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Passive Force
0.00
0.00
0.00
0.00
0.00
0.00
0.02
0.25
1.75
Total Force
1.82
1.75
1.55
1.21
0.73
0.11
1.77
1.46
1.86
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3. Based on the unique arrangement of myosin and actin in skeletal muscle sarcomeres, explain why active force varies
with changes in the muscle's resting length.
Your answer:
Como podemos observar en los experimentos, a medida que aumenta la longitus del musculo la fuerza activa que genera
va aumentando complementandose con la fuerza pasiva.
4. What skeletal muscle lengths generated passive force? (Provide a range.)
Your answer:
longitudes mayor o igual a 80.
5. If you were curling a 7-kg dumbbell, when would your bicep muscles be contracting isometrically?
Your answer:
en base a los experimentos la fuerza generada sera de 1.86 por cada gramo, osea se generara 13020 unidades de fuerza.
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Exercise 2: Skeletal Muscle Physiology: Activity 7: Isotonic Contractions and the Load-Velocity Relationship Lab Report
Pre-lab Quiz Results
You scored 100% by answering 5 out of 5 questions correctly.
1. During an isotonic concentric contraction, the
You correctly answered: b. force generated by the muscle is greater than the weight of the attached load.
2. During an isotonic concentric contraction
You correctly answered: b. the latent period increases with heavier loads.
3. During the latent period for an isotonic concentric contraction
You correctly answered: c. cross bridges cycle and, when muscle tension exceeds the load, muscle shortening occurs.
4. Muscle shortening velocity
You correctly answered: a. decreases with heavier loads.
5. An isotonic contraction of a muscle is one in which
You correctly answered: a. the length of the muscle changes.
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Experiment Results
Predict Question:
Predict Question: As the load on the muscle increases, what will happen to the latent period, the shortening velocity, the
distance that the weight moves, and the contraction duration?
Your answer : b. The latent period will increase, the shortening velocity will decrease, the distance will decrease, and the
contraction duration will decrease.
Stop & Think Questions:
What kind of contraction did you observe when you attached the 2.0 gram weight to the skeletal muscle and stimulated a
contraction?
You correctly answered: c. isometric
Experiment Data:
Voltage
Length
Weight
8.5
8.5
8.5
8.5
75
75
75
75
0.5
1.0
1.5
2.0
Velocity
(mm/msec)
0.100
0.057
0.022
0.000
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Twitch Duration
(msec)
78.00
49.00
30.00
0.00
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Distance Lifted
(mm)
4.0
2.0
0.5
0.0
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5. Explain why it would take you longer to perform 10 repetitions lifting a 10-kg weight than it would to perform the same
number of repetitions with a 5-kg weight.
Your answer:
las musculos que levantaran 10 repeticiones de 10kg comparadas con las de 5 kg, tendran una menor velocidad y una
mayor concentracion isotonica.
6. Describe what would happen in the following experiment: A 2.5-g weight is attached to the end of the isolated whole
skeletal muscle used in these experiments. Simultaneously, the muscle is maximally stimulated by 8.5 volts and the platform
supporting the weight is removed. Will the muscle generate force? Will the muscle change length? What is the name for this
type of contraction?
Your answer:
El musculo genera ms fuerza cuando un peso acorta su longitud isotonica; pero si el peso es superior al de la capacidad
de fuerza que puede ejercer el musculo, este no podra ser levantado.
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