WORKSHOP

SEPARATION AND
PURIFICATION STRATEGIES FOR
BIOTECHNOLOGY PRODUCTS

The aim of a purification procedure should be to isolate a

given enzyme with the maximum possible yield, based on
the percentage recovered activity compared with the total
activity in the original extract. In addition, the preparation
should possess the maximum catalytic activity, i.e. there
should be of the maximum possible purity, i.e. it should
contain no other enzymes or large molecules. In the early
days of enzyme purification, crytallinity was taken to be
proof of purity but there are now known to be a number of
crystalline enzyme that are impure, and indeed the majority
of present-day purification procedures do not involve a
crystalization step.

Activities of workshop:

The steps involved in the purification of an enzyme can be

adopted in general scheme for a given enzyme will involve
choice of: (i) source of enzyme; (ii) methods of
homogenation; and (iii) methods of separation.

Date
Place

Most purification protocols require more than one step to

achieve the desired level of product purity. This includes
any conditioning steps necessary to transfer the product
from one technique into conditions suitable to perform the
next technique. Each step in the process will cause some loss
of product. Consequently, to reach the targets for yield and
purity with the minimum number of steps and the simplest
possible design, it is not efficient to add one step to another
until purity requirements have been fulfilled. Occasionally
when the sample is ready available purify can be achieved
by simply adding or repeating steps. However, experience
shows that, even for the most challenging applications, high
purity and yield can be achieved efficiently in fewer than
four well-chosen and optimised purification steps.
Technique should be organised in a logical sequence to
avoid the need for conditioning steps and chromatograpic
techniques selected appropriately to use as few purificationn
steps as possible.

Registration:

1.

Lectures provide background that helps participants

understand the theory and principles of separations
and purification strategies

2.

Hands-on exercises include cell disruption, centrifugation, membrane filtration, chromatography techniques, and column packing

3.

Discussion with your problem in laboratory

Venue:

Workshop is limited only for 20 participants.

We strongly recommend you to register online at
http://cieb2010.biokatalis.net
Registration fee:
Peserta

BIOCATALYST PRODUCTION TECHNOLOGY DIVISION

Laboratory for the development of agroindustrial

and biomedical technology
(LAPTIABBPPT)
Serpong, 4-6 Agustus 2010

Sebelum 10
Juli 2010

11 - 31 Juli 2010

On Site Registration

Seminar
Umum

400,000

500,000

Mahasiswa

200,000

300,000

600,000

Workshop Teknik Separasi dan Purifikasi Produk Bioteknologi

Umum

1,200,000

1,400,000

Mahasiswa

1,100,000

1,200,000

Trainee benefit:
Hands-on exercises include cell disruption, centrifugation,
membrane filtration, chromatography techniques utilizing
AKTA Explorer, and column packing. The exercises allow
participants to integrate theory with practice and to understand practical issues in purification of biotechnology products.

: Agustus 4 6, 2010
: 610 building, Laboratory for the
development Agroindustrial and Biomedical
Technology (LAPTIAB)-BPPT, PUSPIPTEKSerpong, Tangerang, Banten, Indonesia

1,500,000
Paket Seminar + Workshop Separasi/Purifikasi

Umum

1,500,000

Mahasiswa

1,200,000

Accomodation:
Puspiptek Guest House fare: Single : 162.000 IDR,
Double: 184.000 IDR, Triple: 226.000 IDR
We can reserve it for you exclude registration fee

Program schedule:

Traineers:

Registration form
WORKSHOP

Day One

Maggy T Suhartono, Prof., Dr.

SEPARATION AND PURIFICATION

Lecture:

Agriculture Bogor University

STRATEGIES FOR BIOTECHNOLOGY

Fundamentals of separation techniques, centrifugation

Theory and principles of cell disruption
Hands-on Laboratory:

PRODUCTS
Siswa Setyahadi, Dr.

Cell disruption and centrifugation

Day Two
Lecture:

Puspiptek, Serpong, 4-6 Agustus 2010

Agency for the Assessment and Application of Technology

Ir. Achmadin Luthfi, MEng

Agency for the Assessment and Application of Technology

Name

Job

Institution :

Protein purification methods, affinity separation, and

new separation methods

Ir. Elisabeth Maria, MSi

Address

PT. Sentra Bioscience Dinamika.

Novel separation techniques

Reverse phase chromatography

Budiasih Wahyuntari, Dr.

Centrifugation
Process scale chromatography

Agency for the Assessment and Application of Technology

Optimization, automation, and scale-up

Telp:
Fax:
Please tick Puspiptek Guest House for me :

Downstream processing

Day Three

O Single 162.000 IDR

Lecture:

O Triple 226.000 IDR

Chromatographic techniques

O Double 184.000 IDR

Registration fee transferred at :

Gel filtration, affinity, ion exchange, hydrophobic

interaction

(DD/MM/YY) to Mandiri Bank KCP Jakarta,

Application of membrane processes in biotechnology

Menara Thamrin,

Hands-on Laboratory:
Developing downstream process
Optimization
Scale-up considerations
Practical exercises
Column packing

Rek. No. :103-00-0537226-9

c.q. Astutiati Nurhasanah.

Secretariat
Puspiptek building 610, LAPTIAB-BPPT,
Puspiptek Serpong, Tangerang-Banten.
Mobile

: 081283113440

Email

: maria_umma@yahoo.com

Website

: http://cieb2010.biokatalis.net

(this form and transferred registration fee form

send via email or fax to CIEB 2010 secretariat)
. , ...2010

cieb2010@yahoo.com

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