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Review
Novel disinfectants
for fresh produce
Kompal Joshia, R. Mahendrana,
K. Alagusundarama, T. Nortonb
and B.K. Tiwaric,*
a
Introduction
The fresh produce industry continues to be one of the
most important and ever-growing sectors of the global
food market. However, fresh produce such as fruits and
vegetables are considered significant carriers of pathogenic
55
many fruit juices such as apple cider, strawberry and blackberry. Ozone treatment of juices is found to achieve 5 log
reduction which is mandatory FDA requirement. Studies
show that ozone can be employed either in aqueous and
gaseous form to achieve desired microbial safety (Cullen,
Tiwari, ODonnell, & Muthukumarappan, 2009). Water
flushed with ozone is used for washing of fruits and vegetables. Minimally processed fruits and vegetables can be
treated with aqueous ozone for providing protection against
microorganisms. Ozone-containing water was found to
reduce bacterial content in shredded lettuce, blackberries,
grapes, black pepper, broccoli, carrots and tomatoes
(Kim, Yousef, & Dave, 1999; Sarig et al., 1996; Tiwari,
Muthukumarappan, ODonnell, Cullen, & Rice, 2012). A
delay in the growth of green and blue mould is reported
for ozone treated citrus fruit (Palou, Smilanick, Crisosto,
& Mansour, 2001). Microbial studies typically show a
2 log reduction of total counts and significant reductions
in spoilage and potentially pathogenic microorganisms
commonly associated with fresh produce.
Gaseous ozone can be used as a disinfectant during on
farm storage of fresh produce. Gaseous ozone is reported
to be effective against microorganism during storage was
effectively found to be used on peas and beans (Naito,
Okada, & Sakai, 1988), however change in surface colour
of some products such as peaches and carrots is reported
when treated with ozone. Ozone during storage is also
found to eliminate odour and reduce the spoilage caused
by microorganisms. Studies have shown that the effect of
ozone during storage is variable and depends on the type
of microorganism, characteristics of fresh produce and prevailing storage conditions. For example, Forney, Song, Fan,
Hildebrand, and Jordan (2003) observed a decay resistance
towards Bacillus cinerea in carrots treated with 1000 nl/l
ozone for 2 or 4 days, however they did not observe a decay
resistance towards Sclerotiorum sclerotiorum. Similarly,
Skog and Chu (2001) reported that an ozone concentration
of 0.04 ml/l has the potential to extend the storage life of
broccoli and cucumbers stored at 3 C as compared to 4 C.
Ozone washing of fruits and vegetables is also reported
to degrade pesticide residues. Wu, Luan, Lan, Hung Lo,
and Chan (2007) observed that rinsing at a dissolved ozone
concentration of 1.4 mg/L for 15 min effectively removes
27e34% of residual pesticide from vegetables. Higher
degradation of pesticides residues can be obtained with
an increase in ozone concentration but with added effect
Chlorine
34.04 C
101.5 C
0.007 g/ml
3.2 g/L (at 0 C)
11.7
1.36
Chlorine dioxide
11 C
59 C
8 g/L (at 20 C)
2.757 g/L
3.0
1.57
Ozone
112 C
192 C
0.570 g/L (at 20 C)
2.144 g/L (at 0 C)
7.5
2.1
Peroxyacetic acid
107 C
Completely soluble
1.0375 g/ml
8.2
1.81
H2O2
0.43 C
150.2 C
Miscible
1.450 g/cm3 (20 C pure)
11.75
1.8
56
Bacteria
Fungus
Virus
Spores
Pesticide residue
Chlorine dioxide
Hydrogen peroxide
Peracetic acid
Ozone
U
e
U
U
e
U
e
U
U
e
U
e
U
U
e
U
U
U
U
Moderate
U
U
e
Disinfection technique
Dose/Concentration
Salient finding/
Results
Reference
Lettuce
Electrolyzed water
Radish sprouts
Acidic electrolyzed
water
Electrolyzed oxidizing
water
Alkaline electrolyzed
water and 1% citric
acid
Ozone
2 ppm
Bean sprouts
Carrots
Ozone
Ozone
2 ppm
80 mg/min for 20 min
Tomatoes
Gaseous ozone
Shredded lettuce
Baby carrots
Strawberry
Cantaloupe
Cucumber
Gaseous ozone
Gaseous ozone
Chlorine dioxide
Chlorine dioxide
Chlorine dioxide
Lettuce
Guava
Apples
Fresh cut leek
Tomato
Chlorine dioxide
Chlorine dioxide
Chlorine dioxide
Peroxyacetic acid
Peroxyacetic acid
100 ppm
100 ppm
100 ppm
250 ppm
50 ppm
Lettuce
Fresh cut apple
Peroxyacetic acid
Hydrogen peroxide
50 ppm
20 ml/L
Fresh cut
cantaloupes
Hydrogen peroxide
Spinach
Shredded carrots
4 log CFU/g
(Olmez
& Kretzschmar, 2009). Chlorine dioxide possesses
strong bactericide and virucide properties at low concentrations of 0.1 ppm (http://www.safeox.com/chlorine-dioxideclo2). With minimal contact time, it is highly effective
against many pathogenic organisms including bacterial
spores, Legionella, Tuberculosis, Listeria, Salmonella,
amoebal cysts, Giardia cysts, E. coli, and Cryptosporidium.
Importantly, chlorine dioxide is also effective against biofilm hence, bacterial regrowth is significantly retarded.
Chlorine dioxide penetrates the cell wall of microorganisms
and inhibits metabolic function. It is more efficient than
other oxidizing agent such as chlorine that just burn the surface of whatever they come in contact with. Unlike ozone
ClO2 is very stable compound. Chlorine dioxide is more
effective as it can work on range of pH making it more versatile. It has limited reactions with the water as it remains
as a true gas when dissolved in water, thus making it effective over wide pH range produce unlike chlorine. Comparatively ClO2 is highly stable and less corrosive than ozone
and chlorine. It can also remove the unwanted taste
and odour associated with food products. Chlorine
dioxide is also reported to reduce microbial populations
in dump tank and wash water (Sapers, 2001). As a disinfectant, the use of ClO2 in wash water cucumbers resulted
in less than a 90% population reduction on product
surfaces (Sapers, 2001). Chlorine dioxide vapour phase
disinfection of cut green pepper, inoculated with E. coli
O157:H7, showed to achieve about 6.45 log unit population
reduction (Sapers, 2001). Sy, Murray, Harrison, and
Beuchat (2005) tested the efficacy of gaseous chlorine
dioxide at 4.1 ppm to reduce Salmonella on different
types of fresh produce. Reductions resulting from this
treatment were 3.13e4.42 log CFU/g for fresh-cut
cabbage, 5.15e5.88 log CFU/g for fresh-cut carrots,
1.53e1.58 log CFU/g for fresh-cut lettuce, 4.21 log CFU/
apple, 4.33 log CFU/tomato, 1.94 log CFU/onion, and
3.23 log CFU/peach. Chlorine dioxide gas (ClO2) is a novel
and effective method for minimizing pathogens on fresh
produce without producing potentially harmful carcinogenic compounds (Rodgers, Cash, Siddiq, & Ryser,
2004). Washing of apples showed a 5.5 log CFU reduction
of L. monocytogenes on apple skin by treatment with
57
58
59
60
Conclusion
Recently it has been observed that there is a shift in the
diet of human beings to fresh fruits and vegetables. Fruit
and vegetables must at least undergo minimal processing
to guarantee produce that is safe and of high quality. It is
highly important that the produce is free from microbial
contamination with minimum with no deleterious effect
on the quality of the fresh produce. Chlorine, the most
commonly used disinfectant, has serious health hazards
leading to formation of carcinogenic compounds in produce. Some other novel and efficient disinfectants used
for decontamination include chlorine dioxide, ozone,
hydrogen peroxide, per acetic acid/peroxyacetic acid, electrolyzed oxidizing water. This paper demonstrated that
these are safe for usage and does not leave any residue
behind. Moreover, the paper showed that there is a new
emerging trend in industry which is the hurdle technology
or combination of different chemicals with capabilities to
achieve higher levels of safety in fresh produce.
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