Final Program and Proceedings - 5th International Regional "Stress and Behavior" Neuroscience and Biopsychiatry Conference (North America), June 22-24, 2015, Miami, FL, USA

International STRESS AND BEHAVIOR Society (ISBS)
ZENEREI Institute, USA
Program and
Proceedings
5th International Regional (North America)
ISBS Neuroscience and Biological Psychiatry
Stress and Behavior

Conference
Miami, FL, USA

June 22-24, 2015
5th Regional Stress and Behavior ISBS Conference, June 22-24, 2015, Miami, FL, USA
IN PARTNERSHIP WITH:
2
CONFERENCE PROGRAM
Day 1. Mon, June 22, 2015
Royal Ballroom, Courtyard Miami Downtown/Brickell Area, 200 SE Second Avenue, Miami, FL
09.00-17.00
REGISTRATION
Morning session
10.00-10.30
ISBS OPENING AND WELCOMING. INDUCTION OF ISBS FELOWS
10.30-11.30
ISBS OPENING PLENARY LECTURE: UNDERSTANDING PTSD FROM

BIOLOGICAL, COGNITIVE AND EVOLUTIONARY PERSPECTIVES. DM
Diamond, Departments of Psychology, Molecular Pharmacology and Physiology,
University of South Florida, and Research and Development Service, J.A. Haley
VA Hospital, Tampa, FL, USA
11.30-12.00
ISBS PRESIDENTIAL LECTURE: CROSS-DOMAIN PARSING OF GENETICS

OF COMPLEX NEUROPSYCHIATRIC DISORDERS: FROM DOMAIN GENES
TO ANGEL GENES? AV Kalueff, ISBS Fellow, Research Institute for Marine
Drugs and Nutrients (RIMDN) and College of Food science and Technology,
Guangdong Ocean University, Zhanjiang, China; ZENEREI Institute, New Orleans,
USA; Institute for Translational Biomedicine, St. Petersburg State University, St.
Petersburg, Russia
12.00-12.40
ISBS SPECIAL FOCUS TALK: NORIBOGAINE MULTITARGET-DIRECTED

PHARMACOLOGY: A CANONICAL RECIPE FOR ADDICTION AND ANXIETY
RELATED DISORDERS? EL Maillet, DemeRx Inc., R&D Laboratory, Miami, FL,
USA
12.40-13.50
LUNCH BREAK (FREE TIME) AND EXHIBITION
Afternoon session
13.50-17.40
ZUKOWSKA SYMPOSIUM ON STRESS NEUROSCIENCE

Chairs: D Diamond (USA), S Nakamura, ISBS Fellow (Japan)
13.50-14.00
INTRODUCTION: PROFESSOR ZOFIA M ZUKOWSKA
14.00-14.15
BEHAVIORAL INHIBITION AND UNCERTAINTY ENHANCE CLASSICAL

EYEBLINK CONDITIONING: SUPPORT FOR A LEARNING DIATHESIS MODEL
OF ANXIETY DISORDERS. MT Allen, CE Myers, RJ Servatius, University of
Northern Colorado, Greeley, CO, Stress and Motivated Behavior Institute, Newark,
NJ, Department of Veterans Affairs, NJ Healthcare System, East Orange, NJ, NJ
Medical School, Rutgers University, Newark, NJ, USA
14.15-14.30
NEUROTROPHIC FACTOR-1 PREVENTS STRESS-INDUCED DEPRESSION

THROUGH FGF2 MEDIATED NEUROGENESIS. Y Cheng, Section on Cellular
Neurobiology, Program on Developmental Neuroscience, Eunice Kennedy Shriver
3
National Institute of Child Health and Human Development (NICHHD), NIH,

Bethesda, MD, USA
14.30-14.45
HEART
RATE
VARIABILITY
ENHANCED
DURING
MINDFULNESS
MEDITATION
FOLLOWING
STRESS-INDUCTION:
A
STRATIFIEDRANDOMIZED TRIAL WITH MALADAPTIVE PERFECTIONISTS. M Abid Azam,
York University, Toronto, Ontario, Canada
14.45-15.00
PHARMACOLOGICAL STRESSOR ENHANCES MOTIVATION FOR FOOD

REWARD IN SATIATED RATS: IMPLICATIONS FOR OVEREATING AND
OBESITY. X Liu, Department of Psychiatry and Human Behavior, University of
Mississippi Medical Center, Jackson, MS, USA
15.00-15.15
META-ANALYSIS OF THE EFFICACY AND SAFETY OF PRAZOSIN VERSUS

PLACEBO FOR THE TREATMENT OF NIGHTMARES AND SLEEP
DISTURBANCES IN ADULTS WITH POST-TRAUMATIC STRESS SYMPTOMS.
L Ruth, K George, L Kebejian, S Himelhoch, Department Of Psychiatry, University
of Maryland Medical School, Baltimore, MD, USA
15.15-15.30
A PIVOTAL ROLE OF OREXIN (HYPOCRETIN) NEURONS IN STRESSINDUCED THERMOGENESIS. T Kuwaki, Department of Physiology, Kagoshima
University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
15.30-16.00
COFFEE BREAK AND EXHIBITION
16.00-16.35
THE IMPACT OF FLOTATION RESTRICTED ENVIRONMENTAL STIMULATION

THERAPY (REST) ON HEART RATE, CORTISOL, AND PERCEIVED STRESS
LEVELS. G Talley, A Jahromi, Float On LLC and Float Conference, Portland, OR,
USA
16.35-16.50
EFFECT OF STRESS ASSOCIATED NEUROTRANSMITTERS ON THE

MEDIODORSAL PREFRONTAL CORTEX. B Nagy, I Szab, B Csetnyi, E
Hormay, Z Kardi, University of Pcs, Medical School, Institute of Physiology,
Pcs, Hungary
16.50-17.10
EFFECTS OF AIR TRAVEL STRESS ON THE CANINE MICROBIOME A PILOT

STUDY. EB Venable, SD Bland, HD Holscher, KS Swanson, Department of Animal
Science Food and Nutrition, Southern Illinois University, Department of Animal
Sciences, University of Illinois at Urbana-Champaign, IL, USA
17.10-17.40
PRESENTATION: BIOSEB EB INSTRUMENTS: MJ Craddock, Bioseb In Vivo

Research Instruments, Pinellas Park, FL, USA
Social Program:
7pm Miami Boat Tour (admissions)
4
Day 2. Tue, June 23, 2015
09.00-17.00
REGISTRATION
Morning session
10.00-10.30
ISBS LECTURE: NEUROPSYCHOLOGICAL DEVELOPMENT IN INTERACTION

BETWEEN PHYSICAL AND BIOLOGICAL ENERGY. M Koshiba, ISBS Fellow, G
Karino, K Mimura, H Tokuno, S Usui, I Tanaka, Y Honda, T Kodama, K Sato, H
Kishino, M Shukuya, T Kunikata, S Nakamura, ISBS Fellow, H Yamanouchi,
Saitama Medical University, Saitama, Tokyo University of Agriculture and
Technology, TMIM, NCNP, University of Tokyo, Tokyo City University, Tokyo,
Japan
10.30-12.30
LAPIN SYMPOSIUM ON BIOLOGICAL PSYCHIATRY

Chairs: M Koshiba, ISBS Fellow (Japan), AV Kalueff, ISBS Fellow (USA)
10.30-10.40
INTRODUCTION: PROFESSOR IZYASLAV (SLAVA) P LAPIN
10.40-10.55
THE
EFFECT
OF
DEXMEDETOMIDINE
ON
MORPHINE-INDUCED
DEPENDENCE IN RATS. TB Uskur, MA Barlas, AG Akkan, Istanbul University,
Faculty of Medicine Cerrahpasa, Medical Pharmacology, Istanbul, Turkey
10.55-11.10
SELF-OBSERVATION, REFLECTION AND COPING WITH BRAIN CHEMISTRY

IN ADOLESCENT COMPETING TENNIS PLAYERS. PW Wightman, H Anselmi,
Argentina National Training Center Elite Athletes, University L Zamora, Lomas
Zamora, Argentina
11.10-11.35
11.35-12.00
ASSESSING GENDER DIFFERENCE IN AGGRESSION AMONG AUTISTIC

CHILDRENS PARENTS. R Mittal, Civil Hospital, Moga, Punjab, India
12.00-12.15
TELOMERASE DYSREGULATION IN THE HIPPOCAMPUS OF A RAT MODEL

OF DEPRESSION. NORMALIZATION BY LITHIUM. Y Wei, L Backlund, G
Wegener, A Mathe, C Lavebratt, Neurogenetics Unit, Center for Molecular
Medicine (CMM), Karolinska University Hospital, Stockholm, Sweden
12.15-12.30
PROTEIN BASED DIAGNOSTIC BIOMARKERS FOR EARLY DIAGNOSIS OF

ALZHEIMERS DISEASE. DP Katare, RJ Mani, Center of Medical Biotechnology,
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, India
12.30-14.00
LUNCH BREAK (FREE TIME) AND EXHIBITION
5
Afternoon session
14.00-18.00
INTERACTIVE POSTER SESSION
DO SINGLE EXPERIENCES OF CHILDHOOD ABUSE INCREASE DEPRESSION AND

ANXIETY SYMPTOMS IN ADULTHOOD? W Rehan, A Johansson, P Santtila, Department of
Psychology and Logopedics, Abo Akademi University, Turku, Finland
COMPARING PSYCHOLOGICAL MORBIDITY AMONG STUDENTS APPEARING FOR

FINAL PROFESSIONAL MBBS EXAMINATION AND CIVIL SERVICES EXAMINATION. A
Sharma, M Singla, BS Sidhu, Department of Psychiatry, Guru Gobind Singh Medical College
and Hospital, Faridkot, Punjab, India
IMPACT OF CHILDHOOD ADVERSITY AND VASOPRESSIN RECEPTOR 1A VARIATION

ON SOCIAL INTERACTION IN ADULTHOOD: A CROSS-SECTIONAL STUDY. J Liu, C
Lavebratt, Y Forsell, School of Nursing, Shandong University, Jinan, China; Neurogenetics
Unit, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm,
Sweden
EFFECTS OF MOBILE TECHNOLOGY USE ON MOOD AND BODY MASS INDEX. CB

Shay, C Hansen, Keiser University, Ft. Lauderdale, FL, USA
AMYGDALA INVOLVEMENT IN AVOIDANCE ACQUISITION IN A BEHAVIORALLY

INHIBITED RAT STRAIN. DP Miller, KM Moench, KCH Pang, RJ Servatius, Carthage
College, Kenosha, WI, Stress and Motivated Behavior Institute, Newark, NJ, Department of
Veterans Affairs, NJ Healthcare System, East Orange, NJ, NJ Medical School, Rutgers
University, Newark, NJ, USA
EEG BIOFEEDBACK AND LAVENDER AROMATHERAPY IN MIGRAINE - A

PRELIMINARY STUDY. HH Bauer, SM Nagel, College of Human Medicine, Michigan State
University, East Lansing, MI; Department of Psychology, Saginaw Valley State University,
University Center, MI, USA
EARLY LIFE STRESS AND THE BRAIN-GUT-MICROBIOTA AXIS: IMPACT OF N-3

POLYUNSATURATED FATTY ACIDS. MM Pusceddu, S El Aidy, P Kelly, JF Cryan, TG
Dinan, Department of Psychiatry, Alimentary Pharmabiotic Centre, Moorepark Food Research
Centre, Teagasc, Fermoy Co., Department of Anatomy and Neuroscience; University College
Cork, Cork, Ireland
THE USE OF MODERN TECHNOLOGY DURING TRAINING OF COGNITIVE PROCESSES.

M Zubanska, A Bonus-Dziego, AK Zubrzycka, Police Academy in Szczytno, Szczytno, Poland
ENRICHED ENVIRONMENT DECREASED ANXIETY-RELATED BEHAVIOR IN AN

ANIMAL MODEL OF GENERALIZED ANXIETY DISORDER. GP Dias, ACD Silveira, MCN
Bevilaqua, AA Marques, G Cocks, J Landeira-Fernandez, S Thuret, AE Nardi, Universidade
Federal do Rio de Janeiro UFRJ; Pontifcia Universidade Catlica, Rio de Janeiro, Brazil;
Kings College London, London, UK
INCREASED RISK OF SEXUALLY TRANSMITTED DISEASES AFTER BEREAVEMENT

A SWEDISH NATIONAL COHORT. E Bond, D Lu, E Herweijer, K Sundstrom, U
Valdimarsdottir, K Fall, LA Dahlstrom, P Sparn, F Fang, Department of Medical
Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden
6
THE CYSTIN/GLUTAMATE ANTIPORTER AS A POTENTIAL NOVEL TARGET TO

MODULATE THE STRESS RESPONSE? T Demuyser, E Bentea, L Deneyer, G Albertini, J
Van Liefferinge, E Merckx, A Massie, I Smolders, Center for Neurosciences, Vrije Universiteit
Brussel, Brussels, Belgium
SEEKING SAFETY: A RANDOMIZED CLINICAL TRIAL. C Hansen, D Hien, Keiser

University, Ft. Lauderdale, FL, Columbia University, City College of New York, NY, USA
DEVELOPMENT AND IN VITRO CHARACTERIZATION OF NEUROMEDIN U ANALOGS. A

De Prins, C Betti, B Sivertsen, V Caveliers, A Van Eeckhaut, B Holst, S Ballet, I Smolders,
Department of Pharmaceutical Chemistry and Drug Analysis, Department of Organic
Chemistry, Department of In Vivo Cellular and Molecular Imaging, Vrije Universiteit Brussel,
Brussels, Belgium; Department of Neuroscience and Pharmacology, University of
Copenhagen, Copenhagen, Denmark
PSYCHOLOGICAL STRESS IN EARLY LIFE AFFECTS ADULT RATS IN A SEXDEPENDENT MANNER. SR Melo, CTD Antoniazzi, S Hossain, B Kolb, State University of
Maringa, Parana, Brazil
EVENT-RELATED POTENTIALS FROM PATIENTS WITH MAJOR DEPRESSIVE

DISORDER WITH MELANCHOLIC FEATURES AND ANXIOUS DISTRESS. MV Pronina,
GY Poliakova, YI Poliakov, VA Ponomarev, A Mller, JD Kropotov, NP Bechtereva Institute of
the Human Brain RAS, Saint-Petersburg, Russia
16.30-17.00
THE IMPACT OF MATERNAL TRAUMA HISTORY ON PEDIATRIC ASTHMA CONTROL. M

Corneille, I Barreiro, S Behbahani, Albizu University, Doral, FL, USA
PHARMACOGENETIC TESTING SHOWS UTILITY FOR PSYCHIATRIC PATIENTS. H

Harris, R Scott, K Gardner, J Lombard, FX Brennan, Genomind Inc., Chalfont, PA, USA
CRY OVER THE PAST OR STRESS ABOUT THE FUTURE? TIME PERSPECTIVES
PREDICT PAIN PERCEPTION AND CATASTROPHIZATION. B Gacs, A Csatho, University
of Pecs, Medical School, Department of Behavioral Sciences, Pecs, Hungary
SIMULTANEOUS EXPOSURE TO CHRONIC MILD STRESS AND ENRICHED DIETS

ENHANCE SPATIAL MEMORY VIA DIFFERENTIAL EXPRESSIONS OF DOPAMINE-4
AND METABOTROPIC GLUTAMATE-5 RECEPTORS. PD Shallie, OF Shallie, AK Adefule,
Department of Anatomy, Olabisi Onabanjo University, Ago-Iwoye, Nigeria
EXAMINING THE RELATIONSHIP BETWEEN JOB STRESS, JOB SAFETY, AND JOB
SATISFACTION. SM Guy, Keiser University, Ft. Lauderdale, FL, USA
IMPLICATIONS OF HEALTH CARE COSTS ON CARDIOVASCULAR DISEASE

PATIENTS: A PSYCHONEUROIMMUNOLOGY PERSPECTIVE. I Barreiro, M Corneille, S
Behbahani, ClinPharm, Albizu Universitry, Doral, FL, USA
EXAMINING THE ROLE OF COMMUNICATION AND SOCIAL SUPPORT AS A MEDIATOR

OF STRESS RESILIENCE AMONG CLINICAL PSYCHOLOGY DOCTORAL STUDENTS. JC
7
Lyons, SR Webster, CA Feldman, B Nierenberg, Center for Psychological Studies, Nova

Southeastern University, Ft. Lauderdale, USA
PERCEIVED DISCRIMINATION, ACCULTURATIVE STRESS, AND DEPRESSIVE

SYMPTOMS IN MEXICAN AMERICANS: FAMILISM AND ETHNIC IDENTITY AS
MODERATORS. H-L Cheng, JL Rislin, X Nguyen, C Williams, CH Cha, B Stamper, R
Zamora, GA Liu, J Peters, New Mexico State University, Las Cruces, NM, USA
ULTRASONIC VOCALIZATION DURING EXPLORATION IN BOTH SAFE AND

STRESSFUL ENVIRONMENT IN ADULT MALE MICE. H-S Mun, JC Roder, T Lipina,
Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Department of Molecular
Genetics, University of Toronto, Toronto, ON, Canada
EXAMINING
BEHAVIORAL
DIFFERENCES
BETWEEN
WILD-CAUGHT
AND
DOMESTICATED (LABORATORY) ZEBRAFISH, DANIO RERIO. AV Kalueff, ISBS Fellow, S
Homechaudhuri, C Song, S Li, Y Liu, A Mitra, S Pal, A Chaudhuri, A Roy, M Biswas, D Roy, A
Podder, A Kaluyeva, P Chen, L Yang, JJ Wang, AM Stewart, ISBS Fellow, Research Institute
for Marine Drugs and Nutrients, College for Food Science and Technology, Guangdong
Ocean University, Zhanjiang, China; Department of Zoology, University of Calcutta, Kolkata,
India; ZENEREI Institute and the International Zebrafish Neuroscience Research Consortium
(ZNRC), New Orleans, USA; Institute for Translational Biomedicine, St. Petersburg State
University, St. Petersburg, Russia
Social Program:
7pm Conference Dinner (admissions)
8
Day 3. Wed, June 24, 2015
09.00-13.00
REGISTRATION
Morning session
10.00-15.00
9th INTERNATIONAL ZEBRAFISH NEUROSCIENCE SYMPOSIUM

WORKSHOP
Chairs: AV Kalueff, ISBS Fellow (USA), DJ Echevarria, ISBS Fellow (USA)
10.00-10.10
INTRODUCTION: THE INTERNATIONAL ZEBRAFISH NEUROSCIENCE RESEARCH

CONSORTIUM (ZNRC)
10.10-11.00
ZNRC LECTURE: ZEBRAFISH BIOLOGICAL PSYCHIATRY. DJ Echevarria,

ISBS Fellow, Department of Psychology, University of Southern Mississippi,
Hattiesburg, MS, USA
11.00-11.45
ZNRC PRESENTATION: FROM A TO Z: ACTIVITY ANALYSIS AND DETAILED

OBSERVATIONS IN ZEBRAFISH. J Rogers, Noldus Information Technology Inc.,
Leesburg, VA, USA
11.45-12.15
ZNRC LECTURE: MODELING ACUTE AND CHRONIC STRESS IN ADULT

ZEBRAFISH AN UPDATE. AV Kalueff, ISBS Fellow, S Li, Y Liu, P Chen, L
Yang, JJ Wang, A Kaluyeva, AM Stewart, ISBS Fellow, C Song, Research Institute
for Marine Drugs and Nutrients (RIMDN) and College of Food Science and
Technology, Guangdong Ocean University, Zhanjiang, China; ZENEREI Institute
and the International Zebrafish Neuroscience Research Consortium (ZNRC), New
Orleans, USA; Dalhousie University, Halifax, Canada; Institute for Translational
Biomedicine, St. Petersburg State University, St. Petersburg, Russia
12.15-12.45
AND
Afternoon session
12.45-13.15
ZNRC PRESENTATION: VIEWPOINT. P-A Fel, ViewPoint, Montreal, Canada
13.15-13.30
ASSESSING THE EFFECTS OF LIGHT DARK MANIPULATION AND CAFFEINE

EXPOSURE ON ZEBRAFISH SLEEP BEHAVIOR. KM Khan, DJ Echevarria, ISBS
Fellow, NR Lodinger, AD Collier, Department of Psychology, University of Southern
Mississippi, Hattiesburg, MS, USA
13.30-13.50
DISC1 MODULATES THE STRESS RESPONSE IN LARVAL ZEBRAFISH VIA

HYPOTHALAMIC GENES. HL Eachus, J Wood, M Placzek, PJ Watt, Department
of Animal and Plant Science, Bateson Centre, Department of Biomedical Science,
Sheffield Institute for Translational Neuroscience, Department of Neuroscience,
University of Sheffield, Sheffield, UK
9
13.50-14.05
THE EFFECTS OF METHYLENE BLUE ON LEARNING ACQUISITION IN THE

ZEBRAFISH. EM Caramillo, DJ Echevarria, ISBS Fellow, Department of
Psychology, University of Southern Mississippi, Hattiesburg, MS, USA
14.05-14.25
ADULT ZEBRAFISH AND CONDITIONED PLACE PREFERENCE AS A MODEL

OF DRUG REWARD. AD Collier, NR Lodinger, KM Khan, EM Caramillo, DJ
Echevarria, ISBS Fellow, Department of Psychology, University of Southern
Mississippi, Hattiesburg, MS, USA
14.25-15.00
ROUND TABLE AND ASK-THE-EXPERT SESSION: FUTURE OF ZEBRAFISH

MODELS IN TRANSLATIONAL NEUROSCIENCE RESEARCH
15.00-15.20
CLOSING CEREMONY
ANNOUNCING FORTHCOMING ISBS CONFERENCES
10
CONFERENCE
ABSTRACTS
11
Day 1. Mon, June 22, 2015
Morning session
ISBS OPENING PLENARY LECTURE: UNDERSTANDING PTSD FROM BIOLOGICAL, COGNITIVE
AND EVOLUTIONARY PERSPECTIVES.
DM Diamond, Departments of Psychology, Molecular Pharmacology and Physiology, University of South
Florida, and Research and Development Service, J.A. Haley VA Hospital, Tampa, FL, USA
Post-traumatic stress disorder (PTSD) is routinely described as a pathological or dysfunctional state
because the persistent post-trauma symptoms, such as hyper-vigilance, increased startle response,
intrusive memories, impaired sleep quality and a predisposition toward substance abuse cause traumatized
people to suffer a reduced quality of life. In this presentation I will address the more global issue of whether
the brain should be considered to be acting in a dysfunctional manner in a traumatized person that has
developed PTSD. I will propose that there is heuristic value in considering that in the subset of traumatized
people that develops PTSD, the brain is so protective of the individuals survival that it acts in a hyperfunctional, rather than a dysfunctional, manner. More generally, I will suggest that PTSD symptoms reflect a
hyper-protective status of the immune, cardiovascular, cognitive and neurobiological components of the
stress response, which are influenced by ones genetic predisposition to maximize survival in response to a
life-threatening attack. Therefore, all aspects of the stress response, and even the PTSD state, are adaptive
from an evolutionary perspective, but are maladaptive in modern society because the unbridled and
incessant preparation for a future attack takes its toll on ones physical and mental health.
ISBS PRESIDENTIAL LECTURE: CROSS-DOMAIN PARSING OF GENETICS OF COMPLEX
NEUROPSYCHIATRIC DISORDERS: FROM DOMAIN GENES TO ANGEL GENES? AV Kalueff, ISBS
Fellow, Research Institute for Marine Drugs and Nutrients (RIMDN) and College of Food science and
Technology, Guangdong Ocean University, Zhanjiang, China; ZENEREI Institute, New Orleans, USA;
Institute for Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia
Contemporary biological psychiatry uses both clinical and pre-clinical (animal) models to improve our
understanding of brain pathogenesis. Modeling human psychiatric disorders is currently performed by
targeting various key neurobehavioral clusters (domains) of phenotypic traits, such affective, cognitive,
social, motor and reward. Analyses of such domains and their 'smaller units' (individual endophenotypes)
are critical for the study of complex brain disorders and their neural underpinnings. The spectrum nature of
brain disorders and the importance of pathogenetic linkage among various disordered domains or
endophenotypes have also been recognized as an important strategic direction of translational research
(Kalueff et al., 2015). I will critically discuss cross-domain analyses of animal models, and focus on their
utility for mimicking the clinical overlap between disordered neurobehavioral domains in humans, and their
potential role in discovering common (shared), unique (domain-specific) and novel putative interlinking
(domain interplay) genes. In addition to novel putative class of bad pathogenic domain interplay genes, I
will also propose the existence of protective angel interplay genes, which may exert protective effects
either by 1) improving the cross-talk between genetic mechanisms of resistance or by 2) inhibiting the
pathogenic function of putative disease-enhancing interplay genes.
ISBS SPECIAL FOCUS TALK: NORIBOGAINE MULTITARGET-DIRECTED PHARMACOLOGY: A

CANONICAL RECIPE FOR ADDICTION AND ANXIETY RELATED DISORDERS? EL Maillet, DemeRx
Inc., R&D Laboratory, Miami, FL, USA
Noribogaine is the stable and long-lived metabolite of ibogaine, the principal active of the herbal
hallucinogen Iboga (ibogaine), which has demonstrated lasting beneficial effects in the domain of substance
abuse in humans. Therapeutic potential of noribogaine itself was confirmed in pre-clinical studies where it
attenuated the reinforcing properties of multiple substances of abuse including morphine, nicotine, cocaine
and alcohol in rodents. It was also shown to reduce the pursuit or administration of nondrug reinforcers and
12
restore sensitivity to morphine in tolerant rodents. The mechanism of action was not defined but data
suggested a canonical activity reminiscent of a multiplex modulation of the systems governing reward and
disappointment. Noribogaine is, in fact, a polypharmacological drug which targets multiple neurotransmitter
systems. Molecular characterization of its activity at its principal central targets was recently performed.
Noribogaine displayed physiologically relevant potencies at inhibiting the 3-containing and 7 nAChR
function, non-competitively inhibiting serotonin reuptake as well as displaying a profound biased agonism at
the kappa opioid receptors which may correlate to a specific functional inhibition of CRF-mediated
dynorphin/kappa dysphoric pathways in vivo. Thus, noribogaine appeared remarkably well-fit to target the
emotional, motivational, and stress-related components of addiction. This pharmacological profile also
constituted a basis for drug repurposing in the domain of mood and anxiety related disorders. Recent
studies showed a direct activity of noribogaine at precluding novelty stress, a.k.a. anxiety in the novel tank,
in zebrafish. The non-sedative anxiolytic action of noribogaine was also confirmed in the context of
repeated nicotine withdrawal in zebrafish, confirming the therapeutic potential of noribogaine for challenging
co-morbid states composite of addictive, mood and anxiety disorder spectra. RESEARCH SUPPORT:
DemeRx, Inc.
Afternoon session
ZUKOWSKA SYMPOSIUM ON STRESS NEUROSCIENCE
Chairs: D Diamond (USA), S Nakamura, ISBS Fellow (Japan)
INTRODUCTION: PROFESSOR ZOFIA M ZUKOWSKA.
This regular ISBS symposium is dedicated to Professor Zofia Zukowska
(1949-2012). Professor Zukowska received her M.D. and Ph.D., trained in
cardiovascular medicine at the Warsaw Medical Academy (Poland). She
pursued post-doctoral training at the NIH, working with such renowned
scientists as Irwin I. Kopin, Scientific Director of NINDS, and Julie Axelrod,
Nobel Laureate. It was during this research period when her interest in
stress and neuropeptides became galvanized. For the last 25 years, she
was a professor (and, later, Chair) of the Department of Physiology and
Biophysics at Georgetown University, before moving to the University of
Minnesota as a new Stress Physiology Center Director. She assessed how
stress affects cardiovascular and metabolic health and diseases, and the
role of peptides, in particular neuropeptide Y (NPY), a sympathetic
neurotransmitter and a stress mediator. She was the first to determine that
NPY mediates stress-induced prolonged vasoconstriction and vascular mitogenic and pro-atherosclerotic
effects (via Y1 receptors) and potent angiogenic actions (via Y2 receptors), establishing the role of NPY in
ischemia, retinopathy, tumors and obesity. Professor Zukowska was a strong supporter of ISBS and our
conferences. Her scientific vision, extraordinary creativity, kindness to colleagues, and the talent to be
daring, continue to inspire her ISBS colleagues and their research.
BEHAVIORAL INHIBITION AND UNCERTAINTY ENHANCE CLASSICAL EYEBLINK CONDITIONING:
SUPPORT FOR A LEARNING DIATHESIS MODEL OF ANXIETY DISORDERS. MT Allen, CE Myers, RJ
Servatius, University of Northern Colorado, Greeley, CO, Stress and Motivated Behavior Institute, Newark,
NJ, Department of Veterans Affairs, NJ Healthcare System, East Orange, NJ, NJ Medical School, Rutgers
University, Newark, NJ, USA
INTRODUCTION: Personality factors, such as behavioral inhibition, have been found to enhance
associative learning in classical eyeblink conditioning and may be a diathesis for anxiety disorders. We
recently reported that enhanced acquisition of conditioned eyeblinks in individuals self-reporting behavioral
inhibition was more evident in an omission / yoked protocol in which the US was omitted on a CR trials
(Holloway et al., 2014) and in partial reinforcement protocols with 50% paired trials (Allen et al., 2014) than
in standard 100% CS-US paired training. In both omission/yoked protocols and partial reinforcement
protocols, there is some degree of uncertainty as to when the next air puff will occur. The exclusion of the
air puff on some trials also extends the inter-trial interval (ITI) between tone-air puff training trials. Therefore,
13
uncertainty and/or trial spacing effects may underlie our previous findings of enhanced associative learning
in behaviorally inhibited individuals. In the current study, we tested the hypotheses that spacing trials by
extending the ITI from 30 s to 57 s and by varying the ITI between 25 and 123 s would facilitate learning.
METHODS: Eighty nine participants completed personality inventories (e.g., the Adult Measure of
Behavioral Inhibition). Participants were grouped as behaviorally inhibited (i.e., anxiety vulnerable) and noninhibited based on a median split of the AMBI scores. Delay eyeblink training consisted of 30 paired CS-US
trials (500 ms/1200 Hz pure tone CS overlapping and co-terminating with a 50 ms, 5 psi corneal air puff
US). Eyeblink responses were measured via silver chloride EMG electrodes. RESULTS AND
DISCUSSION: Anxiety vulnerable individuals exhibited facilitated acquisition as compared to nonvulnerable individuals. Behaviorally inhibited individuals exhibited facilitated learning to the spaced trials
with a variable ITI ranging from 25 to 123 s, but not with the 30 s or fixed 57 s ITI. Overall, enhanced
sensitivity to forming stimulus associations in anxiety vulnerable individuals is most evident when the
predictive relationship between the CS and US is uncertain. RESEARCH SUPPORT: This research was
supported by the Stress and Motivated Behavior Institute and UNC.
NEUROTROPHIC FACTOR-1 PREVENTS STRESS-INDUCED DEPRESSION THROUGH FGF2
MEDIATED NEUROGENESIS. Y Cheng, Section on Cellular Neurobiology, Program on Developmental
Neuroscience, Eunice Kennedy Shriver National Institute of Child Health and Human Development
(NICHHD), NIH, Bethesda, MD, USA
Major depressive disorder is linked to stress. Prolonged, but not short-term, stress in mice leads to
depressive-like behavior, indicating the existence of an allostatic mechanism. Here, we elucidated such a
mechanism showing that hippocampal carboxypeptidase E, a new neurotrophic factor also known as NF1, is up-regulated during short-term chronic restraint stress (CRS) in mice (which did not show depressive
like behavior), resulting in enhanced fibroblast growth factor 2 (FGF2) expression and promotion of
neurogenesis in the dentate gyrus, known to alleviate depressive-like behavior. In vitro study showed that
NF-1directly up-regulated FGF2 expression through ERK-Sp1 signaling in primary cultured hippocampal
neurons. Interestingly, Prolonged CRS in mice led to depressive-like behavior as well as decreased
expression of NF-1 and FGF2. Genetic deletion of NF-1 in mice led to decreased hippocampal FGF2
and neurogenesis, and depressive-like behavior. Furthermore, exogenous FGF2 rescued the deficit in
neurogenesis and depressive like behavior in NF-1 knock-out mice. These data identify CPE as a key
modifier during short-term CRS to establish allostasis and prevent depressive-like behavior onset through
enhancement of hippocampal FGF2 expression and neurogenesis. To evaluate the therapeutic potential for
this pathway, we show that the drug rosiglitazone (Rosi), which has been reported to have anti-depressive
activity in mice and humans, up-regulated endogenous NF-1 and FGF2 expression, in a NF-1dependent
manner in hippocampal neurons. Furthermore, Rosi-treated mice showed increased NF-1 level and
neurogenesis in hippocampus, indicating the antidepressant action of this drug. Therefore, NF-1-FGF2
pathway is a novel drug target for treatment of depression. RESEARCH SUPPORT: This research was
supported by the NIH/NICHHD Intramural Program.
HEART RATE VARIABILITY ENHANCED DURING MINDFULNESS MEDITATION FOLLOWING
STRESS-INDUCTION: A STRATIFIED-RANDOMIZED TRIAL WITH MALADAPTIVE PERFECTIONISTS.
M Abid Azam, School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada
INTRODUCTION: Heart rate variability (HRV) is a biomarker of cardiovascular function used to investigate
chronic illness, psychopathology, cognitive stress and, more recently, attention and meditation. This study
investigated HRV in relation to both maladaptive perfectionism (MP), a personality factor associated with
cognitive stress, and mindfulness meditation (MM) - an attention regulation practice expected to promote
cardiovascular recovery signified by increased HRV. METHODS: In a stratified-randomized trial,
perfectionists (n = 21) and controls (n = 39) underwent a lab-based assessment obtaining HRV measures
using electrocardiogram equipment. Data was obtained during a resting phase, a stress-induction phase,
and a post-stress phase which involved randomization to a MM condition or a similar resting condition.
RESULTS AND DISCUSSION: Statistical analyses revealed greater HRV was exhibited in post-stress MM
compared to post-stress rest. There were significant within-subject increases in HRV of controls
randomized to MM after stress-induction. These results support the effectiveness of MM in promoting
cardiovascular recovery following cognitive stress. Further analyses revealed this effect was only present in
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controls and not perfectionists. Psychophysiological models explaining how perfectionistic stress can impair
cardiovascular recovery are discussed. Overall, more research is needed to elucidate the mechanisms of
perfectionism and stress reduction through mindfulness to support the psychological and physiological
health of clinical and subclinical populations.
PHARMACOLOGICAL STRESSOR ENHANCES MOTIVATION FOR FOOD REWARD IN SATIATED
RATS: IMPLICATIONS FOR OVEREATING AND OBESITY. X Liu, Department of Psychiatry and
Human Behavior, University of Mississippi Medical Center, Jackson, MS, USA
INTRODUCTION: Overeating beyond individuals homeostatic needs critically contributes to obesity. The
neurobehavioral mechanisms underlying the motivation to consume excessive foods, especially with high
calories (e.g., containing high-fat contents), are not fully understood. Exposure to stressful life events is
proposed to be an environmental and psychological factors that are present in our ever-changing societies
Here, we examined whether stress enhances the motivation to procure food reward with an emphasis on
comparisons between standard lab chow and high-fat foods. The effects of corticotropin-releasing factor
receptor (CRF1) antagonism on the stress-enhanced motivation for food reward were also assessed.
METHODS: Male Sprague-Dawley rats with chow available ad libitum in their home cages were trained to
press a lever under a progressive-ratio schedule for deliveries of either standard chow or high-fat food
pellets. In the test sessions, rats received an intraperitoneal administration of yohimbine. In the rats trained
with high-fat food pellets, the CRF1 receptor antagonist NBI was administered 20 min prior to yohimbine
challenge. RESULTS AND DISCUSSION: The rats emitted higher levels of lever responses to procure the
high-fat food reward compared with their counterparts on standard chow pellets. Yohimbine challenge
facilitated lever responses for the reward in all of the rats, whereas the effect was more robust in the rats on
high-fat food pellets compared with their counterparts on standard pellets. Pretreatment with NBI effectively
attenuated the enhancing effect of yohimbine challenge. Stress challenge significantly enhanced the
motivation to procure food reward, especially the high-fat food. Activation of CRF1 receptors is required for
the stress-enhanced motivation for food reward. These results may have implications for our understanding
of the biobehavioral mechanisms of overeating and obesity. RESEARCH SUPPORT: This work was
supported by NIH Grants R01DA017288 and R01DA037277 from NIDA.
META-ANALYSIS OF THE EFFICACY AND SAFETY OF PRAZOSIN VERSUS PLACEBO FOR THE
TREATMENT OF NIGHTMARES AND SLEEP DISTURBANCES IN ADULTS WITH POST-TRAUMATIC
STRESS SYMPTOMS. L Ruth, K George, L Kebejian, S Himelhoch, Department of Psychiatry, University of
Maryland Medical School, Baltimore, MD, USA
BACKGROUND: Sleep disturbances can have profound deleterious effects on the well-being of adults with
post-traumatic stress symptoms (PTSS), even after adequate trials with antidepressants (Green et al.,
2014). Prazosin can mitigate sleep disturbances and improve overall functioning, yet its use is not
widespread. The aim of this meta-analysis is to assess the efficacy and safety of prazosin versus placebo
for the reduction of nightmares, sleep disturbances, and illness severity in adults with post-traumatic stress
symptoms (PTSS). METHODS: Electronic databases (PubMed, PsycINFO) were searched in September
2014 for randomized controlled trials (RCT). All RCTs assessed the efficacy of prazosin in the treatment of
nightmares and sleep disturbances in adults with PTSS. Of the 102 articles that met selection criteria, six
were retained for review. Two authors independently extracted data for nightmare frequency and intensity,
sleep quality, illness severity, and blood pressure change. RESULTS: Prazosin was more effective than
placebo in improving nightmares (SMD of 0.97, 95% CI 0.47, 1.47, p = 0.000), sleep quality (SMD of 0.93,
95% CI -0.2, 1.88, p=0.054 and SMD of 1.1, 95% CI of 0.21, 1.99, p = 0.02), and illness severity (SMD of
1.15, 95% CI 0.67, 1.64, p = 0.00) and had no significant effect on systolic (SMD of -0.01, 95% CI -0.36,
0.34, p = 0.95) or diastolic blood pressure (SMD of 0.255, 95% CI -0.10, 0.61, p = 0.15). DISCUSSION: The
findings of this meta-analysis are in accordance with those of multiple prior studies in demonstrating that
prazosin is effective for the treatment of post-traumatic stress-related nightmares, sleep disturbances, and
illness severity. Our results expand upon those of a prior meta-analysis by the inclusion of an additional
RCT and differing methodology, both of which positively impacted our effect sizes (Seda et al., 2015).
Further, this study demonstrates that prazosin, when used at doses as high as 15 mg, had no significant
effect on blood pressure. The lack of a change in blood pressure with prazosin use in the context of PTSS
has important implications because the utilization and dosing of the medication may be limited by
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practitioner concern for blood pressure-related side effects. Evidence that its use was not associated with
significant blood pressure change in this analysis may ease concerns about side effects.
THE IMPACT OF FLOTATION RESTRICTED ENVIRONMENTAL STIMULATION THERAPY (REST) ON
HEART RATE, CORTISOL, AND PERCEIVED STRESS LEVELS. G Talley, A Jahromi, Float On LLC
and Float Conference, Portland, OR, USA
Restricted Environmental Stimulation Therapy (REST) is based on reducing the external sensory inputs
going to the brain, including audio, visual, and tactile. Flotation REST is a specific type of REST that
involves floating in a saturated epsom salt and water solution (eliminating most gravity on the body) which is
kept at a skin receptor-neutral temperature. In non-flotation REST, participants are kept in total darkness in
soundproof rooms to block out audio-visual inputs. Flotation REST can have a large impact on participants,
helping to reduce both chronic and acute levels of stress. Here, we discuss the general effects of flotation
REST on stress, as well as the specific impact on heart rate, cortisol concentration, and perceived stress
levels. During the course of a session of flotation REST, cortisol levels (measured intravenously) drop
significantly. Heart rate and breath rate drop off as well, as the session naturally elicits the relaxation
response in participants. Perceived reduction in stress levels generally lasts well after the float session,
from several hours to several days. In addition, pilot results from fMRI studies on flotation REST (currently
performed at the Laureate Brain Institute in Tulsa, OK) will be discussed. RESEARCH SUPPORT: Float On
LLC and Float Conference (Portland, OR, USA).
A PIVOTAL ROLE OF OREXIN (HYPOCRETIN) NEURONS IN STRESS-INDUCED THERMOGENESIS. T
Kuwaki, Department of Physiology, Kagoshima University Graduate School of Medical and Dental
Sciences, Kagoshima, Japan
Stress increases cardiac function, ventilation, and body temperature. These changes prepare the body for
fight-or-flight behavior by increasing the metabolic rate, oxygen supply, and by conduction velocity of nerve
impulses. A key role of one subregion of the hypothalamus, so called defense area, has long been known
but precise mechanisms and/or subserving neurotransmitters remain largely unknown. Our recent research
results shed light on orexin (hypocretin) neurons as a master switch that triggers multiple components of the
fight-or-flight (defense) response (Kuwaki and Zhang, 2010). As an extension of this research, we
suggested that the stress-induced hyperthermia would also depend on orexin. We used handling stress
model in which a temperature probe was repetitively inserted into the animals rectum. We found, contrary
to our expectations, orexin neuron-ablated mice (ORX-AB) but not orexin knockout mice (ORX-KO) have a
blunted stress-induced hyperthermia. The brown adipose tissue, which is a major thermogenic organ in
rodents, did not respond to handling stress although it did respond to a direct pharmacologic stimulation.
These abnormalities in ORX-AB were not observed in ORX-KO in which orexin peptide is deficient but
neurons are preserved. In wild-type mice and ORX-KO, handling stress activated orexin neurons (as
revealed by increased expression of c-Fos) and the resultant hyperthermia was largely blunted by
pretreatment with a beta-3 antagonist. These observation further supports the notion that attenuated stressinduced hyperthermia in ORX-AB mice was caused by a loss of orexin neurons and abnormal BAT
regulation. A similar abnormality in ORX-AB but not in ORX-KO was observed when other types of
stressors, namely cold (but not hot) exposure and central administration of prostaglandin E2, were applied.
Therefore, the integrity (orexin and co-existing other neurotransmitter/modulators) of the orexin neurons is
indispensable for full expression of multiple facets of the fight-or-flight response including thermogenesis,
irrespective of the stressor types so far examined.
EFFECT OF STRESS ASSOCIATED NEUROTRANSMITTERS ON THE MEDIODORSAL PREFRONTAL
CORTEX. B Nagy, I Szab, B Csetnyi, E Hormay, Z Kardi, University of Pcs, Medical School, Institute
of Physiology, Pcs, Hungary
INTRODUCTION: The mediodorsal prefrontal cortex (mdPFC), constituent of the forebrain glucosemonitoring (GM) system and a major behavioral regulator, plays important role in the control of stress
responses. The GM neurons are intimately involved in regulatory functions, such as ingestive behavior,
taste perception and metabolism. Mild stressors increase the extracellular concentration of dopamine (DA),
noradrenaline (NA) and acetylcholine (Ach) in the PFC. Little is known, however, about the neurochemical
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sensitivity of neurons located in this area. Our research focuses on stress associated neurochemical
characteristics, especially glucose, DA, NA and Ach sensitivities of mdPFC neurons. METHODS: In the
present study, extracellular single neuron activity of the mdPFC of anesthetized rats was recorded by
means of multi-barreled glass microelectrodes during microelectrophoretic administration of D-glucose, DA,
NA and Ach. RESULTS AND DISCUSSION: 25% of the mdPFC cells, the GM neurons, displayed firing
rate changes to microiontophoretic administration of D-glucose. DA responsiveness of the GM cells (41.2%)
was significantly higher than that of the glucose-insensitive (GIS) units (16.2%). One fifth of the neurons
tested changed activity to microiontophoretic application of NA, responsiveness of the GM cells to this
catecholamine also proved to be significantly higher than that of the GIS units. Microiontophoretic
application of Ach resulted in activity changes of more than 40% of the mdPFC neurons, with similar
proportion of GM and GIS Ach sensitive cells. The characteristic DA and NA sensitivities of GM neurons in
the mdPFC may have particular significance in the organization of adaptive behavioral responses to
stressful and other environmental challenges. RESEARCH SUPPORT: This research was supported by the
Campus Hungary Programme, Ajinomoto 51064/2009 and PTE OK KA 2013/34039/1.
EFFECTS OF AIR TRAVEL STRESS ON THE CANINE MICROBIOME A PILOT STUDY. EB Venable,
SD Bland, HD Holscher, KS Swanson, Department of Animal Science Food and Nutrition, Southern Illinois
University, Department of Animal Sciences, University of Illinois at Urbana-Champaign, IL, USA
INTRODUCTION: Travel stress in companion dogs is associated with diarrhea, but is poorly understood in
working canines. Behavior assessment is commonly used to identify those canines which may have a
higher threshold for environmental stressors, but no work has been done establishing a connection between
behavior (as indicated by search performance) and travel stress (as indicated by fecal scores and
microbiome stability). METHODS: Six canines (aged 18 months to 8 years), trained according to the
standard established by the Federal Emergency Management Agency (FEMA), were utilized to test the
effects of airline travel stress on working canines. Institutional Animal Care and Use Committee approval
(#14-005) was obtained prior to the initiation of the study. All dogs were assessed for body condition score
(BCS) at 4.5 0.5. Our objectives were to test the hypotheses that 1) working canines can overcome air
travel stress with little or no impact on their performance, and 2) that the fecal score and subsequent
microbial profile is impacted by airline travel stress. Two groups of dogs (3/group) were randomly selected
from FEMA canine teams rostered in New York City, NY (CONTROL) and in Miami, FL (TRAVEL). Dogs
from the TRAVEL group were flown in cabin on a commercial airline to New York (2.5-h flight time) and
blood and fecal samples were collected each morning prior to travel (d 1) and search work (d 2-4).
Complete blood chemistry panels were performed (VetScan 2, Abaxis Inc.). Fecal bacterial DNA was
extracted (Mo Bio DNA Extraction Kits) and 16S rRNA amplicon sequencing was performed (Illumina,
MiSeq) followed by bioinformatics analysis using QIIME 1.8.0. Data were analyzed using SAS (version 9.2;
SAS Institute, Inc.). Significance was established at (P < 0.05). Fecal scores were assessed daily (Purina
Fecal Scoring System, Nestle-Purina, St. Louis MO). RESULTS AND DISCUSSION: Fecal scores from
TRAVEL were significantly higher than control (P = 0.01). BUN for the TRAVEL group was significantly
higher than the CONTROL group (P = 0.01), which may be a result of the dehydration often observed in
canines during travel. There was no effect of treatment on creatinine (P > 0.05). Principal coordinates
analysis (PCoA) of UniFrac distances between samples based on their 97% OTU composition indicated
that TRAVEL bacterial communities (P = 0.01) and bacterial community abundances (P = 0.02) were
significantly different from CONTROL. Search performance (as reported by FEMA certified handlers) was
not impacted by travel. These data demonstrate that airline travel of 2.5 hours impacts the working canine
gut microbiota, but has no impact on working canine performance. Further work is needed to identify the
physiological impact of the microbiological shifts on the canine gut. RESEARCH SUPPORT: This research
was funded by Southern Illinois University.
AN FMRI ANALYSIS OF CORTISOL AND CENTRAL SENSITIZATION: PRELIMINARY EVIDENCE FOR
CORTISOL-INDUCED NEUROPLASTIC ALTERATIONS THAT MAY CONTRIBUTE TO THE
TRANSITION FROM ACUTE TO CHRONIC PAIN. KE Hannibal, MD Bishop, University of Florida,
Gainesville, Florida
Activity-dependent facilitation of glutamate-mediated excitatory postsynaptic potentials (EPSPs) and
upregulation of brain-derived neurotrophic factor (BDNF) during central sensitization may augment synaptic
plasticity and potentiate long-term neuroplastic alterations in pain circuitry. Cortisol secretion may facilitate
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persistent central sensitization and neuroplasticity by increasing glutamate and its NMDA receptors,
prolonging calcium uptake, and upregulating BDNF and other neurotrophic factors. Similarly, cortisol
secretion during an acute pain experience may potentiate neuroplastic alterations in pain circuitry that may
underlie the transition from acute to chronic pain. This preliminary study analyzes the relationship between
cortisol and increases in central sensitization during a minor episode of acute musculoskeletal pain.
METHODS: 9 pain-free volunteers between 20-25 years old underwent a validated exercise protocol
designed to induce delayed-onset muscle soreness (DOMS) to the lower back musculature. Central
sensitization was assessed using fMRI during a common measure of temporal summation (TS), whereby
repeated pulses of static suprathreshold heat were applied to the bottom of the right foot. Participants were
told they would be randomized to 1 of 3 manual therapy interventions designed to treat low back pain. All
participants received sham intervention, and central sensitization (TS during fMRI) was reassessed
immediately thereafter. Salivary cortisol was collected immediately following fMRI scans. RESULTS:
Overall, salivary cortisol concentration was associated with increases in hemodynamic responsiveness
(HRF) in the pain processing regions of the brain during TS as compared to rest (p<.001 FWE-corrected).
Pre- vs post- intervention within groups analysis revealed associations between salivary cortisol and
increases in HRF in the hypothalamus, hippocampus, basal ganglia, insula, and culmen following sham
intervention (p<.001 FWE-corrected). DISCUSSION: These findings provide preliminary evidence for the
impact of cortisol on central sensitization, which may represent the primary stages of neuroplasticity. Given
the gene-mediated effects of cortisol on neuroplasticity, cortisol secretion during an acute pain experience
may facilitate the transition to chronic pain. RESEARCH SUPPORT: This research was supported by the
National Institute of Health (NIH, NCCIH, AT006334).
Day 2. Tue, June 23, 2015
Morning session
ISBS LECTURE: NEUROPSYCHOLOGICAL DEVELOPMENT IN INTERACTION BETWEEN PHYSICAL
AND BIOLOGICAL ENERGY. M Koshiba, ISBS Fellow, G Karino, K Mimura, H Tokuno, S Usui, I Tanaka,
Y Honda, T Kodama, K Sato, H Kishino, M Shukuya, T Kunikata, S Nakamura, ISBS Fellow, H
Yamanouchi, Saitama Medical University, Saitama, Tokyo University of Agriculture and Technology, TMIM,
NCNP, University of Tokyo, Tokyo City University, Tokyo, Japan
Our biological life emerges as energy modules in the alterative but certainly fluctuant energy fields of
environment. The forms of relevant energy include thermal, electric, magnetic action and gravitation that
influence our daily lives and the environments. Designing novel interventions for psychiatric disease and reconsidering the energy circulation and balancing in various kinds of dynamics may, therefore, be necessary.
METHODS: Here, we examined neuropsychological development by assessing behavioral, physiological
and psychological modulations in three species - humans, domestic chicks and common marmosets - with
simultaneous recording of various external (environmental) parameters. We currently used this approach to
the first stage of perinatal development in the environments of our neonatal intensive care unit (NICU). This
work was approved by the institutional review board in Saitama Medical University (13-092). RESULTS
AND CONCLUSION: We found synergistic interactions between social behaviors and visual, auditory and
tactile senses in experimental animal development. Their neuronal changes were observed in anatomical,
physiological and molecular levels. The physical energy alterations, such as thermal and light rhythmic
environment, further revealed their correlation with biological dynamics. These data suggests that statistical
methodology development must be crucial to achieve a comprehensive understanding of energy balancing
systems. RESEARCH SUPPORT: This work was supported by JSPS KAKENHI Grant Numbers 25282221,
21200017, 25119509, 15K15404 and JST-ALCA, JST-a-step Japan.
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SYMPOSIUM ON BIOLOGICAL PSYCHIATRY

Chairs: M Koshiba, ISBS Fellow (Japan), AV Kalueff, ISBS Fellow (USA)
INTRODUCTION: PROFESSOR IZYASLAV (SLAVA) P LAPIN
This regular ISBS symposium is dedicated to Professor Izyaslav (Slava) P.
Lapin (1930-2012), one of the true pioneers of experimental
neuropsychopharmacology. He graduated from Pavlov Medical School in
St. Petersburg, and shortly after receiving PhD, was invited in 1960 to
establish the first psychopharmacology laboratory at the Bekhterev
Psychoneurological Institute. The most important scientific contribution of
Prof. Lapin was establishing the link between serotonin levels and moodelevating (thymoleptic) action of antidepressants. He suggested that
enhanced central serotoninergic tone is essential for the mood-elevating
effects of antidepressants. Lapins serotonin hypothesis of antidepressant
action, published in Lancet in 1969, became one the most cited papers
published in this journal in the last 50 years. Lapins studies have
contributed greatly to the development of newest serotonergic antidepressants, such as SSRIs, currently
representing the most prescribed group of psychotropic drugs in the world. Prof. Lapin was also the first to
report the neuroactive effects of kynurenine and its derivatives a discovery that opened another rapidly
expanding area of glutamatergic psychopharmacology. A talented professional musician, prolific writer,
painter, and an enthusiastic athlete, Prof. Lapin was a strong supporter of ISBS, and generously shared his
knowledge at our Stress and Behavior conferences and ISBS summer schools. His enthusiasm,
friendship, generous support of junior colleagues, and the deep knowledge as both a clinical and
experimental neuropharmacologist (humanists and animalists, as he called them), made a long-lasting
impact on his colleagues and students.
THE EFFECT OF DEXMEDETOMIDINE ON MORPHINE-INDUCED DEPENDENCE IN RATS. TB Uskur,
MA Barlas, AG Akkan, Istanbul University, Faculty of Medicine Cerrahpasa, Medical Pharmacology,
Istanbul, Turkey
OBJECTIVES: Numerous studies, indicated that alpha-adrenergic system may interact with the opioid
system. So, the present study was designed to investigate the effect of alpha adrenoceptor agonist
Dexmedetomidine on Morphine dependent rats and its potential as drug abuse by using conditioned place
preference (CPP) test. METHODS: In the following study, all groups were evaluated for reinforcement and
rewarding effects by using conditioned place preference test. Male Wister Albino rats weighing 250-300 g
were used in the experiment. The animals were divided into six groups as follows: 1. Control (Physiological
Serum), 2. 10 mg/kg morphine, 3. 10 g/kg dexmedetomidine, 4. 20 g/kg dexmedetomidine, 5. 10 g/kg
dexmedetomidine + 10 mg/kg morphine, 6. 20 g/kg dexmedetomidine + 10 mg/kg morphine. The
experiment was counducted for the total period of 13 days with 4 different stages; habituation, pretest,
conditioning and preference test. Conditioning phase was followed for 10 days, 4 days conditioning, 2 days
rest and again 4 days conditioning. In the test phase, the rewarding effects were evaluated. Statistical
analysis was done by using one way analysis of variance (one way ANOVA) followed by Newman-Keuls.
RESULTS: Morphine significantly enhanced the preference scores in the drug paired side (p<0.01), while
rats treated with serum physiological solution did not show any significant effect. In addition to this, both the
doses of dexmedetomidine showed strong acquisition of CPP (p<0.01). Significant reduction in preference
scores were observed with 10 g/kg dexmedetomidine+10 mg/kg morphine (p<0.01). Whereas, 20 g/kg
dexmedetomidine +10 mg/kg Morphine showed non-significant reduction in preference scores than that of
Morphine group (p >0.05). CONCLUSIONS: The acquisition of CPP with dexmedetomidine 10 g/kg and
20 g/kg was demonstrated for the first time in this experiment. 10 g/kg dexmedetomidine + 10 mg/kg
morphine reduced the morphine-induced CPP. A nonsignifcant reduction was observed in 20 g/kg
dexmedetomidine + morphine which can be speculated as 20 g/kg dexmedetomidine being ineffective or
reinforces the effect of morphine or reversal of acquisition of CPP at low dose of dexmedetomidine +
morphine. The following data suggests that dexmedetomidine plays an important role in morphine addition.
Further research is required in terms of dexmedetomidine rewarding properties as well as its potential as
drug abuse.
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SELF-OBSERVATION, REFLECTION AND COPING WITH BRAIN CHEMISTRY IN ADOLESCENT

COMPETING TENNIS PLAYERS. PW Wightman, H Anselmi, Argentina National Training Center Elite
Athletes, University L Zamora, Lomas Zamora, Argentina
INTRODUCTION: Adolescent rapid growth changes, including physiological, psychological, cognitive and
behavioral changes, can often be seen in sport environments and surrounding social situations. Although
the emotions and demands most often cause tension, anxiety and stress, idiographic sport-specific studies
show that athletes not always experience these challenges as negative reactions, but may benefit from
these stimuli as they produce energizing behaviors (Terry, 2013), and thus improve performance. Mental
repercussions due to male and female differences are also important (Brizendine, 2007). The aim of this
study is to detect emotions in competition, register them and identify their effects on performance. Players
were taught to reflect, express, modify or preserve and control these mood states with the help of
professionals. METHODS: Design: Qualitative/descriptive. Fifteen high-level ITF tennis players 12-17 years
old (5 girls and 10 boys), 5 male players who participated in International junior tournaments, Orange Bowl
and Prince Cup in 2013 and 2014. Procedures involved: Recall of emotions and autonomic symptom
associated with best and worst performance (according to Goulds (2010) adaption of Orlicks (1986)
Competitive Reflection Form), STAI, POMS, and semi-directed interviews. CONCLUSIONS: The mood
states of 8 subjects (>50%) were fear and anger during competition, albeit subjects also perceived that,
sometimes and not always, they performed better in training than in competition. This conclusion was
especially considered, and although physical and technical growth contributed to diminishing negative
moods, we conclude that the follow-up with psychological assessment helped (during the two years) to
improve mood, performances and observed behavior.
ASSESSING GENDER DIFFERENCE IN AGGRESSION AMONG AUTISTIC CHILDRENS PARENTS.
R Mittal, Civil Hospital, Moga, Punjab, India
The study was conducted to access the gender difference in aggression among the autistic childrens
parents. Random sampling technique was used and 25 pair of parents (25 males + 25 females), total 50
respondents constituted the sample of study. The Consumable Booklet of A.S by Drs Guru Pyari Mathur
and Raj Kumari Bhatnagar was used as a tool, consisting of 55 statements related to behavior
characteristics which the subjects demonstrate in different situations. The t-test was used for statistical
analyses. The mean score and t-score of males were, respectively, 187.37 and 1.07. The mean score and
t-score of females were, respectively, 193.3 and 5.65. We found that autistic childrens mothers were more
aggressive than fathers due to their over-loaded work extra care, emotional attachment to their children,
societal and domestic pressure as well as social/economic status.
TELOMERASE DYSREGULATION IN THE HIPPOCAMPUS OF A RAT MODEL OF DEPRESSION.
NORMALIZATION BY LITHIUM. Y Wei, L Backlund, G Wegener, A Mathe, C Lavebratt, Neurogenetics
Unit, Center for Molecular Medicine (CMM), Karolinska University Hospital, Stockholm, Sweden
INTRODUCTION: Telomeres are protective DNA-protein complexes at the ends of each chromosome,
maintained primarily by the enzyme telomerase. Shortening of the blood leukocyte telomeres (LT) is
associated with aging, several chronic diseases and stress, e.g. major depression. Hippocampus is pivotal
in the regulation of cognition and mood and the main brain region of telomerase activity. Whether there is
telomere dysfunction in the hippocampus of depressed subjects is unknown. Lithium, used in the treatment
and relapse prevention of mood disorders, was found to protect against LT shortening in humans, but the
mechanism has not been elucidated. To answer the questions whether telomeres are shortened and the
telomerase activity changed in the hippocampus and whether lithium could reverse the process, we used a
genetic model of depression, the Flinders Sensitive Line (FSL) rat and treated the animals with lithium.
METHODS: Telomere length (TL), telomerase reverse transcriptase (Tert) expression,telomerase activity
and putative mediators of telomerase activity were investigated in the hippocampus of these animals.
RESULTS AND DISCUSSION: The nave FSL had shorter TL, downregulated Tert expression, reduced
BDNF levels and telomerase activity compared with the Flinders Resistant Line (FRL) controls. Lithium
treatment normalized the Tert expression and telomerase activity in the FSL, and upregulated -catenin.
This is the first report showing telomere dysregulation in hippocampus of a well-defined depression model
and restorative effects of lithium treatment. If replicated in other models of mood disorder, the findings will
contribute to understanding both the telomere function and the mechanism of lithium action in hippocampus
20
of depressed patients. FUNDING: This study was supported by grants from the Karolinska Institutet, the
Swedish Medical Research Council (grants 2010-3631 CL and 10414 AAM), the Sderstrm-Knigska
Foundation, the Regional Agreement on Medical Training and Clinical Research (ALF) between Stockholm
County Council and Karolinska Institutet, the Danish Medical Research Council and the Lundbeck
Foundation.
PROTEIN BASED DIAGNOSTIC BIOMARKERS FOR EARLY DIAGNOSIS OF ALZHEIMERS DISEASE.
DP Katare, RJ Mani, Center of Medical Biotechnology, Amity Institute of Biotechnology, Amity University
Uttar Pradesh, Noida, India
INTRODUCTION: Alzheimers disease (AD) is one of the most common neurodegenerative disorders that
frequently cause dementia and affect the middle-to-old aged individuals over the age of 65. AD is a complex
progressive condition that involves sequentially interacting pathological cascades, including the interaction
of amyloid-beta aggregation with plaque development, and the hyper-phosphorylation and aggregation of
tau protein with formation of tangles. Together with associated processes, such as inflammation and
oxidative stress, these pathological cascades contribute to loss of synaptic integrity and progressive
neurodegeneration. Brain has multiple sources of ROS, and it cannot reduce oxidative stress by itself.
Today the focus is being placed on the discovery of oxidative stress biomarkers to study the increased
oxidative damage during disease progression. Inadequacy in disease detection/treatment and the lack of
diagnostic and prognostic tools have prompted investigators to turn to proteomics-based biomarker
discovery. Although an emerging field proteomics application promise to uncover biomarkers critical for
differentiating patients with neurodegenerative diseases from healthy people and from patients affected by
other diseases. These studies will also contribute mechanistic information to facilitate identification of new
drug targets for subsequent therapeutic development. In the present study, an animal model of AD was
developed in zebrafish and Wistar rats, and the differential expression of proteins was analyzed in serum
and brain tissue with the disease progression. METHODS: The animal model in zebra fish and male Wistar
rats was developed using STZ toxicant. Behavioral studies like Morris water maize, Tail suspension, novel
object identification, light and dark test were carried out. Biochemical analysis of enzymes of oxidative
stress like Catalase, LPO, SOD etc were done in serum and brain tissue. Next, serum proteins were
screened at regular time intervals of disease progression by 1D SDS PAGE and 2 D Electrophoresis
followed by MALDI-TOF-MS to see the protein expression changes. RESULTS AND DISCUSSION: The
behavior studies and biochemical analysis confirmed AD in the animal model. The analysis of proteins
identified some low molecular weight proteins and some proteins involve in oxidative stress, which were
subjected to further validation for the prospective diagnostic biomarkers. Further characterization of these
proteins will likely shed more light on the mechanisms by which the changes or modification in these
proteins and their interaction with the other protein in the pathway contribute to the development of AD.
RESEARCH SUPPORT: Infrastructure support provided by Amity University Uttar Pradesh Noida.
Afternoon session
21
INTERACTIVE POSTER SESSION

DO SINGLE EXPERIENCES OF CHILDHOOD ABUSE INCREASE DEPRESSION AND ANXIETY
SYMPTOMS IN ADULTHOOD? W Rehan, A Johansson, P Santtila, Department of Psychology and
Logopedics, Abo Akademi University, Turku, Finland
INTRODUCTION: Evidence suggests that abuse experiences in childhood increase the risk for
development of depression and anxiety disorders in adulthood. However, no previous studies have looked
at the effects of single abuse experiences. In the present study, we explored the effects of such
experiences compared to no abuse experiences and to repetitive abuse experiences on depression and
anxiety symptoms in adulthood. METHODS: We used a population-based sample of 10980 participants
(3766 male and 7214 female twins and their siblings). The mean age of the men was 29.2 (SD = 7.4) and of
the women 28.8 (SD = 7.2) years. The participants reported on their abuse experiences using the
Childhood Trauma Questionnaire (CTQ) and on their current depression and anxiety symptoms using the
Brief Symptom Inventory-18 (BSI-18). RESULTS AND DISCUSSION: We found that in both men and
women even single experiences of abuse were associated with the development of increased adult
depression and anxiety symptoms compared to no abuse experiences when looking at the different abuse
types independently, with the exception that in men a single experience of physical abuse did not lead to
increases in either anxiety and depression symptoms. Also, as expected, repeated experiences of abuse
increased both anxiety and depression symptoms compared to a single experience. However, when we
isolated the individuals who had only had a single experience of any type abuse, no increases in anxiety
were found compared to those with no experiences with the exception of depression in women. These
findings suggest that in both men and women that even single experiences of especially psychological
abuse can have long-lasting negative consequences. However, this is mostly explained by comorbidity
between abuse types with the exception of women who seem to be suggestible to increases in depression
from a single experience of abuse in the absence of any other types of abuse. RESEARCH SUPPORT:
This research was supported by the Academy of Finland Grant 210298, a Center of Excellence grant from
the Stiftelsen fr bo Akademi Foundation Grant 21/22/05.
COMPARING PSYCHOLOGICAL MORBIDITY AMONG STUDENTS APPEARING FOR FINAL
PROFESSIONAL MBBS EXAMINATION AND CIVIL SERVICES EXAMINATION. A Sharma, M Singla, BS
Sidhu, Department of Psychiatry, Guru Gobind Singh Medical College and Hospital, Faridkot, Punjab, India
BACKGROUND: Present educational system is most competitive and lesser opportunities in educational
institutes and services later on. High expectations of family and future insecurities among students force
them to take pressure of competitive exams beyond their compromised abilities by various factors. This
leads to psychiatric problems among students at very young age. This study aimed to compare psychiatric
morbidity in students appearing for civil services and final year MBBS examination. MATERIAL AND
METHOD: Two study groups of students for preliminary and mains examinations of civil services were
made and later divided into repeaters and fresher students in each. Students were subjected to structured
and standardized PGI-HQ 1 and SCL-80 scales to assess psychiatric morbidity. Data was collected and
later analyzed using chi square and p value. RESULTS: Clinical diagnosis on ICD-10 revealed that
significantly more (p<0.05) depression (F32) among preliminary students in 28% of cases than only 16% of
mains student. On SCL-80 scale, significantly higher (p< 0.05) number of students in preliminary group had
higher symptoms on anxiety subscale (p< 0.01), on interpersonal sensitivity and anger hostility and
significant number (p<0.05) of preliminary students in 26.67% and 36% had symptoms, though mild as
compared to 13.34% and 14.67% of students from mains group. Repeaters from preliminary group in
significant number (p< 0.05) had higher severity of symptoms than fresher. CONCLUSION: Civil services
exam is one of the toughest exams to take, and subjects students to rigorous physical, psychological and
financial burden which puts them to a high-risk group for various psychiatric problems. Timely intervention
at various levels of stress can make students perform better and keep them healthy psychologically.
IMPACT OF CHILDHOOD ADVERSITY AND VASOPRESSIN RECEPTOR 1A VARIATION ON SOCIAL
INTERACTION IN ADULTHOOD: A CROSS-SECTIONAL STUDY. J Liu, C Lavebratt, Y Forsell, School of
Nursing, Shandong University, Jinan, China; Neurogenetics Unit, Department of Molecular Medicine and
Surgery, Karolinska Institutet, Stockholm, Sweden
22
INTRODUCTION: Arginine vasopressin (AVP) plays a role in social behavior, through receptor AVPR1A.
The promoter polymorphism AVPR1A RS3 has been associated with human social behaviors, and with
acute response to stress. Here, the relationships between AVPR1A RS3, early-life stressors, and social
interaction in adulthood were explored. METHODS: Adult individuals from a Swedish population-based
cohort (n=1871) were assessed for self-reported availability of social integration and social attachment and
for experience of childhood adversities. Their DNA samples were genotyped for the microsatellite AVPR1A
RS3. RESULTS AND DISCUSSION: Among males, particularly those homozygous for the long alleles of
AVPR1A RS3 were vulnerable to childhood adversity for their social attachment in adulthood. A similar
vulnerability to childhood adversity among long allele carriers was found on adulthood social integration, but
here both males and females were influenced. CONCLUSIONS: Early-life stress influences the relationship
between AVPR1A genetic variants and social interaction. For social attachment, AVPR1A is of importance
in males only. The findings add to previous reports on higher acute vulnerability to stress in persons with
long AVPR1A RS3 alleles and increased AVP levels. RESEARCH SUPPORT: This work was supported by
the Swedish Research Council (2009-5546 YF, 2010-3631 CL), the China Scholarship Council, the
Karolinska Institutets Faculty Funds, and the Regional Agreement on Medical Training and Clinical
Research between Stockholm County Council and Karolinska Institutet (SLL20110560 CL, 20090281 YF).
The funders had no role in study design, data collection and analysis, decision to publish, or
preparation of the publication.
EFFECTS OF MOBILE TECHNOLOGY USE ON MOOD AND BODY MASS INDEX. CB Shay, C Hansen,
Keiser University, Ft. Lauderdale, FL, USA
Technology has advanced rapidly in the last decade, especially relating to individual mobile computing
technologies such as smartphones and tablets. Prior research found conflicting results regarding
psychological and physiological effects of these new technologies. This study (n=487) examined effects
mobile technology use had on stress, anxiety, and depression, as defined by the Depression Anxiety Stress
Scale-21 (DASS-21), as well as the effect of technology use on Body Mass Index (BMI). The DASS-21 was
combined with a modified version of a technology-use measure formulated by Wack and Tantleff-Dunn
(2009) to determine possible effects of electronic gameplay on BMI. Data were gathered using Amazons
Mechanical Turk (AMT), a crowd sourcing online service. Results suggest there might be a relationship
between stress in males and higher levels of technology use (here defined as the total of daily time spent
video gaming, in general PC/Internet use, tablet use, and smartphone use). In women, all three of the
DASS-21 subscales showed significant correlation between higher levels of technology use and higher
levels of stress, depression and anxiety. A small correlation between unhealthy levels of BMI and
technology use, including Smartphone and tablet use, was also observed.
AMYGDALA INVOLVEMENT IN AVOIDANCE ACQUISITION IN A BEHAVIORALLY INHIBITED RAT
STRAIN. DP Miller, KM Moench, KCH Pang, RJ Servatius, Carthage College, Kenosha, WI, Stress and
Motivated Behavior Institute, Newark, NJ, Department of Veterans Affairs, NJ Healthcare System, East
Orange, NJ, NJ Medical School, Rutgers University, Newark, NJ, USA
INTRODUCTION: Behavioral inhibition is an important risk factor for the development of anxiety disorders.
The amygdala has been demonstrated to be critical for fear conditioning, and thus it has been suggested
that anxiety disorders can be understood simply by the study of fear conditioning. We have suggested that
the critical nature of avoidance in anxiety disorders requires the study of avoidance in animal models as
well (Servatius et al., 2008). We use the behaviorally inhibited Wistar Kyoto (WKY) rat in comparison to the
outbred Sprague Dawley (SD) strain. Our avoidance paradigm is signaled leverpress, a non-species
specific defense response (non-SSDR) that requires autoshaping during training. We tested the hypothesis
that the basolateral amygdala is necessary for avoidance acquisition while systematically varying the
contingency between the warning signal and avoidance responding. METHODS: In the first experiment an
avoidance leverpress immediately terminated the warning signal and resulted in no shock delivery. In the
second experiment, the warning signal remained on for 1 min regardless of the rats behavior. Thus the
negative reinforcement of the prevention of shock delivery was not immediately contingent upon avoidance
lever pressing. Bilateral electrolytic lesion of the basolateral amygdala and lesion histology was performed
similar to (Lee and Kim, 2004). Escape, avoidance and inter-trial responses were recorded. RESULTS AND
DISCUSSION: Both learning contingency and behavioral inhibition affected whether basolateral amygdala
23
lesions disrupted avoidance acquisition. While lesioned WKY rats showed avoidance acquisition deficits in
both protocols, avoidance acquisition was not disrupted in SD rats in the non-contingent protocol. Our data
support the importance of a diathesis model that takes into account key vulnerabilities that are predictive of
anxiety pathology. These data also demonstrate that manipulations of the learning context allow the
observation of differences in both learning and neural function in vulnerable populations. RESEARCH
SUPPORT: This research was supported by the Stress and Motivated Behavior Institute, UMDNJ (USA).
EEG BIOFEEDBACK AND LAVENDER AROMATHERAPY IN MIGRAINE - A PRELIMINARY STUDY.
HH Bauer, SM Nagel, College of Human Medicine, Michigan State University, East Lansing, MI;
Department of Psychology, Saginaw Valley State University, University Center, MI, USA
INTRODUCTION: Migraine is a common medical condition that is often linked to stress. Two relaxation
techniques, electroencephalographic (EEG) biofeedback and lavender aromatherapy may reduce migraine
severity. The present study tested the hypothesis that migraineurs would experience greater relaxation
when simultaneously exposed to lavender aromatherapy and EEG biofeedback than in response to EEG
biofeedback alone. METHODS: Twenty-two individuals with a chronic history of migraine were recruited
using IRB approved methods. They were assessed on the level of important moderator variables, a selfreport, mood state questionnaire (Profile of Mood States (POMS)), and EEG Biofeedback Training with
(WL) or without (WoL) Lavender Aromatherapy. Raw EEG data (FPZ) were subjected to spectral analysis,
and the power within beta, alpha and theta bandwidths were compared. Slower EEG power indicated
greater relaxation. RESULTS AND DISCUSSION: The WL condition tended to increase positive mood
states over the WoL condition (t=-1.9(df=13), p=0.07). Lavender aromatherapy, therefore, tended to
increase a positive subjective mood in the participants, while EEG biofeedback did not. Individuals in the
WoL condition tended to experience more relaxation as measured by theta power during the biofeedback
training, while the same was true for individuals in the WL condition during the segment following
biofeedback, when they were only exposed to lavender (F(1,18)=3.4, p=0.08). One speculation offered to
account for this trend is that the two relaxation techniques interfere with each other when used
simultaneously. As these methods work through different mechanisms, it is suspected that they work better
alone than together. Furthermore, as the WoL condition showed greater variance in subjective mood
(F(1,18)=9.1, p<0.00) than those in the WL condition, the reflexive, olfactory/limbic mechanisms influenced
by aromatherapy may ultimately yield more effective and predictable relaxation outcomes than the more
cognitively-mediated biofeedback techniques. RESEARCH SUPPORT: This research was supported by the
Saginaw Valley State University Honors Program and Department of Psychology.
EARLY LIFE STRESS AND THE BRAIN-GUT-MICROBIOTA AXIS: IMPACT OF N-3 POLYUNSATURATED FATTY ACIDS. MM Pusceddu, S El Aidy, P Kelly, JF Cryan, TG Dinan, Department of
Psychiatry, Alimentary Pharmabiotic Centre, Moorepark Food Research Centre, Teagasc, Fermoy Co.,
Department of Anatomy and Neuroscience; University College Cork, Cork, Ireland
INTRODUCTION: Stressful life events, especially those in early life, can exert long-lasting changes in the
brain and the brain-gut-microbiota axis, increasing vulnerability to mental illness especially in females.
Omega-3 polyunsaturated fatty acids (PUFAs) play a critical role in the development and function of the
CNS. Thus, we investigated the influence of an eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA)
(80% EPA, 20% DHA) omega-3 PUFA mixture on stress-related behavioral and neurobiological responses.
We also focused on the impact of such PUFAs on the gut-microbiota. METHODS: Sprague-Dawley female
rats were subjected to an early-life stress, maternal separation (MS) procedure from postnatal days 2 to 12.
Non-separated (NS) and MS rats were administered saline, EPA/DHA 0.4 g/kg/day or EPA/DHA 1g/kg/day,
respectively. RESULTS AND DISCUSSION: In adulthood, EPA/DHA dose dependently reduced anxiety in
NS rats. Furthermore, cognitive performance in the novel object recognition task (NOR) was improved by
EPA\DHA treatment. EPA/DHA 1 g/kg/day influenced behavioral despair on the forced swim test (FST).
Notably, EPA/DHA high dose increased the translocation of GRs into the nucleus of NS rat hippocampus.
The corticosterone response to an acute stress was blunted in MS rats and this was further attenuated by
pre-treatment with EPA/DHA. Immune response and monoamine neurotransmission were significantly
altered by early-life stress. A gut microbial imbalance was observed in MS adult rats, and was normalized
by EPA/DHA treatment. In conclusion, our study supports the view that PUFAs are beneficial in
neurodevelopmentally normal animals, and have positive benefit in the microbiota of animals exposed to
early life stress. RESEARCH SUPPORT: Research was funded by Food Institutional Research Measure
24
(FIRM) Grant 10/RD/TMFRC/709, the Alimentary Pharmabiotic Centre (APC) grants 07/CE/B1368 and
12/RC/2273, and the Science Foundation Ireland grant 12/IA/1537.
THE USE OF MODERN TECHNOLOGY DURING TRAINING OF COGNITIVE PROCESSES. M Zubanska,
A Bonus-Dziego, AK Zubrzycka, Police Academy in Szczytno, Szczytno, Poland
In December 2012, Police Academy in Szczytno (Poland) initiated a research project Development of
Training Interactive Psycho-Stimulator for the Police, in order to develop and implement an innovative
diagnostic system for the evaluation of training and competence of cognitive stimulation and psychomotor
skills of police officers. The key tasks of the project include the development of a set of psychological tests
to assess and monitor the psychomotor performance and selected cognitive processes; the development of
a set of computer games that stimulate the development of selected cognitive processes; the development
of a psychophysiological recorder to aid training. The diagnostic system for the evaluation of training and
stimulation of cognitive competence that is being developed consists of three interconnected modules:
diagnostic, training module and knowledge base, within a fully functional web-based platform. Diagnostic
module consists of the four components, each representing a set of psychological tests for independent
measurement of the level of concentration, perception, working memory and analytical thinking. The
developed psychological tests are characterized by internal compliance at the level of 0.60. The training
module is a set of games related to the stimulation of cognitive competence, allowing for online training,
using both PCs and mobile devices as smart phones or tablets. Implementation of the system on the online
platform will allow users to obtain feedback on their training progress in real-time, and to compete with each
other. Knowledge base (i.e. the last module of the system) provides information and articles on the
development of cognitive functions. On the basis of psychological tests, we have designed short tests for
rapid diagnosis of cognitive and psychomotor skills and for determining the level of preparedness in the
area of the measured components to work in particularly difficult situations. As part of the project, a
psychophysiological recorder is also designed for training to cope with stress by using information on the
physiological reactions occurring during both games and solving tests.
ENRICHED ENVIRONMENT DECREASED ANXIETY-RELATED BEHAVIOR IN AN ANIMAL MODEL OF
GENERALIZED ANXIETY DISORDER. GP Dias, ACD Silveira, MCN Bevilaqua, AA Marques, G Cocks, J
Landeira-Fernandez, S Thuret, AE Nardi, Universidade Federal do Rio de Janeiro UFRJ; Pontifcia
Universidade Catlica, Rio de Janeiro, Brazil; Kings College London, London, UK
INTRODUCTION: Anxiety is a complex psychobiological process which often emerges after experiences
perceived as threatening. If the anxious responses are excessive in magnitude or frequency, or if they occur
in the absence of stressors, it can give rise to a pathological condition, such as generalized anxiety disorder
(GAD). In a previous report, we described some of the behavioral characteristics of an animal model for the
study of GAD, the Carioca High Conditioned Freezing rats (CHF). This model was generated after the
selection of Wistar rats with higher freezing in contextual fear conditioning. We verified that these animals
present higher anxiety in the elevated plus maze (EPM) and decreased social interaction, with no
differences in depressive-related behavior or in the object recognition test. We also showed that these
animals present increased serum corticosterone, decreased number of neuroblasts in the hippocampus, as
well as increased expression of hippocampal brain-derived neurotrophic factor and dendritic spines.
However, the search for interventions that could potentially modulate these aspects and restore behavior
had yet to be done. For this purpose, we investigated here the effects of the enriched environment (EE) on
the anxious behavior of CHF and control rats. METHODS: 2-month old males were submitted to the EE for
2 months. The EE consisted of a larger cage, with 12 animals per cage for increased social interaction and
with the presence of stimulating toys and platforms. After the 2-month period in the EE, the animals were
subjected to a single 5-minute trial in the EPM. RESULTS AND DISCUSSION: The time CHF-EE rats spent
in the open arms of the EPM was significantly increased in comparison with CHF rats and similar to that of
control and control-EE animals. Although further behavioral testing is needed in other paradigms, we
observed that the EE in adulthood can be a potentially effective intervention to restore the anxious behavior
in our model. RESEARCH SUPPORT: This study was supported by Research Foundation of the State of
Rio de Janeiro (FAPERJ-Brazil), National Council for Scientific and Technological Development (CNPqBrazil).
25
INCREASED RISK OF SEXUALLY TRANSMITTED DISEASES AFTER BEREAVEMENT A SWEDISH

NATIONAL COHORT. E Bond, D Lu, E Herweijer, K Sundstrom, U Valdimarsdottir, K Fall, LA Dahlstrom, P
Sparn, F Fang, Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm,
Sweden
INTRODUCTON: Although inconclusive, evidence has started to accumulate suggesting a link between
psychological stress and cervical cancer. However, none of the previous studies has addressed potential
underlying pathways, for instance increased sexual risk behavior or compromised immune surveillance.
Therefore we aimed to assess the relationship between bereavement, as a proxy for severe psychological
stress, and the risk of sexually transmitted diseases (STDs). METHODS: We conducted a population-based
cohort study from 1987 to 2012 using the Swedish Multi-Generation Register, including 3,002,209 women
at the age of 10- 44 years who were born in Sweden and had at least one parent recorded in the Register.
Exposure was defined as death of a first degree relative (child, parent or sibling) or spouse before 1987 or
during the study (N=979,579, 33%). STDs were defined as hospital visits, either inpatient or outpatient, with
a STD as main or secondary discharge diagnosis, including condyloma, gonorrhea, chlamydia, syphilis,
human immunodeficiency virus-1, and genital herpes simplex. Poisson regression was used to estimate the
incidence rate ratios (IRRs) and 95% confidence intervals (CIs) of STDs, comparing the incidence rates of
women who had lost a close relative to those who had not. RESULTS AND DISCUSSION: When
compared to women without bereavement, bereaved women were at significantly higher risk of nearly all
STDs studied. The associations appeared slightly stronger for inpatient hospital contact for STDs; for
condyloma for example the IRR was 1.18 (95% CI: 1.08-1.30) for inpatient and 1.06 (95% CI: 1.00-1.13) for
any hospital contact. In contrast, the IRRs of acute salpingitis a severe complication of STDs, did not vary
between inpatient contact (1.28; 95% CI: 1.13-1.44) and any hospital contact (1.27; 95% CI: 1.16-1.38). Our
findings indicate an increased risk of receiving an STD diagnosis after bereavement. While we do believe
that this is mainly due to behavioral changes, the stronger findings on inpatient hospital contact for STDs,
as well as for acute salpingitis a complication of STDs, do suggest that immune modulatory effects of the
stressful life event might also play a role. RESEARCH SUPPORT: This research was supported by the
Swedish Society for Medical Research.
THE CYSTIN/GLUTAMATE ANTIPORTER AS A POTENTIAL NOVEL TARGET TO MODULATE THE
STRESS RESPONSE? T Demuyser, E Bentea, L Deneyer, G Albertini, J Van Liefferinge, E Merckx, A
Massie, I Smolders, Center for Neurosciences, Vrije Universiteit Brussel, Brussels, Belgium
INTRODUCTION: In modern society, stress is a major causative factor for a variety of psychiatric disorders.
Depression, one of the main causes of disability worldwide, is a multimodal disease with chronic stress
considered as a trigger for depressive episodes. Depression and comorbid anxiety are usually related to a
malfunctioning monoaminergic system, nowadays however compelling evidence points at an important role
of glutamate in the etiology of the depressed/anxious brain. Being the major excitatory neurotransmitter in
the central nervous system, glutamate can potentially have important excitotoxic effects. System xc- is the
cystine/glutamate antiporter and the major source of extrasynaptic glutamate in some important depressionrelated brain areas, where it can be an interesting new target for improved psychopharmacological
treatment. METHODS: In this study we investigated the effect of loss of functional system xc- (e.g. deletion
of the specific light chain subunit xCT; xCT-/-), on chronic stress induced depression and anxiety in a
validated animal model. Therefore, we subjected xCT-/- and +/+ mice, treated with chronic corticosterone
injections (excessive chronic stress), to a battery of acute stress-based tests for depressive- and anxietylike behavior, and compared their behavior to vehicle-treated and nave animals. RESULTS AND
DISCUSSION: Interestingly we found decreased depressive- and anxiety- like behavior in the nave xCT-/mice in all of the tests conducted. Unexpectedly however the decrease in depressive- and anxiety-like
behavior faded and disappeared after vehicle and corticosterone treatment. These findings support further
research for the role of system xc- in the stress response, since the involvement of the antiporter in
regulating the response to acute versus chronic stress seems to differ. RESEARCH SUPPORT: This
research was supported by the Fund for Scientific Research Flanders (FWO, grant G.038412N), the Queen
Elisabeth Medical Foundation (GSKE), and the Vrije Universiteit Brussel (Strategic Research Program,
grant SRP40).
SEEKING SAFETY: A RANDOMIZED CLINICAL TRIAL. C Hansen, D Hien, Keiser University, Ft.
Lauderdale, FL, Columbia University, City College of New York, NY, USA
26
INTRODUCTION: Women who seek treatment for substance use disorders are more than twice as likely to
have co-occurring PTSD than men, yet few specific therapies have been developed that integrate treatment
of these co-occurring disorders. Furthermore, many treatment professionals fear that integrating PTSD
intervention may cause adverse reactions that could undermine the substance abuse treatment. The
purpose of this study was to test the effectiveness of Seeking Safety, a cognitive-behavioral integrated
group treatment for PTSD and substance use disorders, in a multi-site, national controlled clinical trial
among women enrolled in intensive outpatient substance abuse treatment in community-based treatment
centers. METHOD: Seeking Safety was compared to a Womens Health Education control as an adjunct to
regular treatment for women with co-occurring diagnoses enrolled in community treatment centers. A total
of 353 women from seven treatment centers were randomized in a National Institutes for Drug Abuse
Clinical Trials Network nationwide study. RESULTS AND DISCUSSION: Participants in both treatment
conditions demonstrated significant improvement in PTSD symptoms over the six-week course of treatment
as well as during the year-long follow-up period; however, there were no significant differences between
conditions. Substance use, measured by abstinence as well as number of days of use per week, did not
improve significantly over the course of the study, and no significant differences were found between
groups. Results support the value of integrating PTSD treatment for those with co-occurring disorders, and
further refinement of treatment is indicated in order to improve substance use outcomes. RESEARCH
SUPPORT: This research was supported by grants from the National Institute on Drug Abuse, NIDA:
U10DA13035 (EV Nunes, PI), U10 DA13714 (D Donovan, PI), U10 DA13038 (K Carroll, PI), U10DA13732
(E Somoza, PI), U10 DA13727 (K Brady, PI), U10 DA013720 (J Szapocznik, PI), U10 DA013046 (J
Rotrosen, PI), and K24 DA022412 (EV Nunes). The Clinical Trial Identification Number is NCT00078156
(NIDA, NIH).
DEVELOPMENT AND IN VITRO CHARACTERIZATION OF NEUROMEDIN U ANALOGS. A De Prins, C
Betti, B Sivertsen, V Caveliers, A Van Eeckhaut, B Holst, S Ballet, I Smolders, Department of
Pharmaceutical Chemistry and Drug Analysis, Department of Organic Chemistry, Department of In Vivo
Cellular and Molecular Imaging, Vrije Universiteit Brussel, Brussels, Belgium; Department of Neuroscience
and Pharmacology, University of Copenhagen, Copenhagen, Denmark
Chronic stress is a predominant risk factor for a variety of psychiatric and neurological disorders, such as
depression, anxiety and epilepsy. Endogenous systems which regulate the stress response are interesting
targets for the development of novel treatments for these stress-related disorders. In this study we focus on
neuromedin U (NMU), a neuropeptide regulator of the stress response via top-down control of the
hypothalamus-pituitary-adrenal axis. NMU exerts its biological effects via two G protein-coupled receptors,
NMUR1 and NMUR2. NMUR1 is mostly found in the periphery whereas NMUR2, the receptor of our
interest, is most abundant in the central nervous system. The purpose of this study is to develop new
peptidergic selective NMUR2 antagonists which are enzymatically stable and blood-brain-barrier (BBB)
permeable. NMU-8, a natural occurring form of NMU, is taken as lead molecule for the synthesis of new
analogues. The NMU-ligands are synthesized via solid phase peptide synthesis under classical conditions
on rink amide polystyrene resin. A first batch of analogues is prepared on basis of the available structureactivity relationships. The in vitro characterization of these peptides is performed by an inositol phosphate
accumulation assay. The results of this in vitro characterization are generally in line with the available
literature. EC50 values of a similar magnitude are found for NMU-8. Moreover our experiments revealed
that acetylation of the N-terminus leads in general to an increase of the relative activity compared to the
non-acetylated ligand. In conclusion, further research is needed to synthesize a NMUR2 selective,
enzymatically stable and BBB permeable ligand. RESEARCH SUPPORT: This research was supported by
the Agency for Innovation by Science and Technology in Flanders (IWT).
PSYCHOLOGICAL STRESS IN EARLY LIFE AFFECTS ADULT RATS IN A SEX-DEPENDENT
MANNER. SR Melo, CTD Antoniazzi, S Hossain, B Kolb, State University of Maringa, Parana, Brazil
INTRODUCTION: Psychological models of stress in different phases of life in laboratory animals have been
used to try to understand the adult signature of early stress in the brain and behavior. Previous studies have
shown that mild stress during gestational, neonatal, and adult periods produce changes in brain and
anxiety-related behavior in adulthood. In this study our goal was to study the relation between the adult
behaviors and brain anatomy as a result of stress in lab rats in different phases of early life. METHODS:
27
Long-Evans male and female rats were stressed by maternal separation (MS) and elevated platform (EP).
We compared the effect of neonatal stress in rats from postnatal days 3 to 14 (P3-14, MS) and juvenile
stress (P23-34, EP) as well the combination of stressors (P3-8, MS + P23-28, EP) on the adult brain (P95).
The elevated plus maze test was used to verify the anxiety-like behavior exhibited by the rats. RESULTS
AND DISCUSSION: The brains of all stressed groups, males and females, were significantly smaller (brain
weight) than control animals. Male but not female rats in the MS and the MS+EP groups showed increased
anxiety-related behavior relative to control animals in an elevated plus maze. EP male rats were not
significantly more anxious than control, in contrast to the females, which showed a reduced level of anxiety.
These results suggest that in both sexes, juvenile rats are more resilient to stress than rats exposed to
neonatal stress. In addition, females are more resilient to neonatal stress than males. Morphological
analysis of cortical pyramidal neurons is in progress. RESEARCH SUPPORT: This research was support
by Capes Brazil and NSERC of Canada.
EVENT-RELATED POTENTIALS FROM PATIENTS WITH MAJOR DEPRESSIVE DISORDER WITH
MELANCHOLIC FEATURES AND ANXIOUS DISTRESS. MV Pronina, GY Poliakova, YI Poliakov, VA
Ponomarev, A Mller, JD Kropotov, NP Bechtereva Institute of the Human Brain RAS, Saint-Petersburg,
Russia
According to DSM-5, major depression disorder (MDD) is specified by melancholic features or anxious
distress. Melancholic features have been defined by criteria related to disturbances in affect, psychomotor
disturbances, cognitive processes and other vegetative dysfunction symptoms, whereas anxious distress is
characterized by high level of anxiety, restlessness, fear and concentration difficulties. Clinical symptoms of
depressive patients, including both with anxiety and melancholic features, may be associated with deficits in
the brain system of cognitive control. This system is suggested to comprise cortical areas and the basal
ganglia/thalamic nuclei. The neuronal correlates of these activations are reflected in event-related potentials
(ERPs) waves recorded in cognitive control tasks for example tasks of Go/NoGo paradigm. The aim this
study was to determine differences of latent event-related potentials in groups of depressive patients with
melancholic features and anxious distress, and also with healthy control group. Subjects were 45 MDD
patients 40-80 years old (25 patients with anxious distress and 20 with melancholic features). We also used
EEG data from 233 healthy subjects of the same age from HBIMed database. Test consisted of 400 probes,
probes were pairs of visual stimuli: animal-animal (probe Go), animal-plant (probe NoGo), plant-plant
(Ignore) and plant-human (Novel). Probes were presented in random order with probability of 25%. Probe
Novel was accompanied by sound. Participants are instructed to press the button as quickly as possible
after Go probe and do not press after other types of stimuli. 19-channel EEG was recorded during test
execution. Button signal was registered to control the reaction time and the amount of mistakes. We applied
the ERPs method of blind source separation based on second-order statistics (Yeredor, 2010) and adapted
for the transient responses (Ponomarev and Kropotov, 2013). Statistical analysis of ERPs, performed using
unpaired t-test between groups of patients, revealed significant difference in only two waveforms. One was
noted right before the second stimuli in Go/NoGo pairs, and was localized in the posterior temporal areas.
This wave may be connected with retention of the first stimuli in memory. The second affected wave with
central localization was found in ERPs after the second stimuli in NoGo probes in the interval of 590-890
ms. Both groups have significantly smaller amplitude of numerous waveforms of ERPs after the first and
second stimuli in Go/NoGo probes, including well-known P300 waves. Our results support the hypothesis of
cognitive system disruption in MDD which is common for patients with different specifiers. This observation
proposes the possibility of using this method for diagnostics of MDD. RESEARCH SUPPORT: This
research is supported by the Russian Foundation for Humanities (grant 14-06-00973a).
THE IMPACT OF MATERNAL TRAUMA HISTORY ON PEDIATRIC ASTHMA CONTROL. M Corneille, I
Barreiro, S Behbahani, Albizu University, Doral, FL, USA
INTRODUCTION: Despite the availability of novel pharmacological approaches to treat asthma, the rate of
suboptimal asthma control still poses a problem to providers and families. Since asthma is often
conceptualized as a psychosomatic illness, due to the known relationship between stress and the trajectory
of asthma, more recent literature is dedicated to identifying factors outside of physiological influences of
asthma control. METHODS: A comprehensive literature review using the EBSCO, Pubmed, Medline, and
NCBI databases was conducted to explore the relationship between psychosocial factors (e.g., trauma) and
asthma control. Selected articles to be reviewed span from 2010 and 2015, and the following keywords
28
directed the search of the selected articles: maternal trauma, asthma control, family environments, patientpractitioner communication. RESULTS AND DISCUSSION: Literature identifies the need to identify factors
outside of physiological influences of asthma control. Research suggests quality of care-taking in infancy
and childhood may be associated with alterations in the HPA axis, and those alterations may affect the
severity of the childs asthma. Psychosocial factors (e.g., maternal trauma) have been identified as having
an impact on asthma management. Maternal trauma is identified as a significant predictor of family
functioning and contributes significantly to ineffective family environments. Patient psychological issues are
found to have a direct impact on asthma control, and negatively impact patient-provider communication.
Individuals with psychiatric disorders are often first identified in the primary care setting, which may be an
optimal, non-stigmatized setting to screen for caregiver trauma histories, as it is universally accessed.
Mothers with reported trauma backgrounds may have increased risks of negatively impacting their
childrens asthma control status due to deficits affecting patient-practitioner communication. Effective
patient-centered communication skills can be applied to guide optimal management of pediatric asthma
control. CONCLUSIONS: Overall, family functioning and more specifically, caregiver psychological
functioning have been identified as a major determinant to childrens asthma outcomes. This review of
literature explores the complex factors associated with both maternal trauma and asthmatic offspring.
Guidelines for practitioners to help identify patients at increased risk for poor asthma control, and increase
effective practitioner-patient interaction in ineffective family environments are suggested.
PHARMACOGENETIC TESTING SHOWS UTILITY FOR PSYCHIATRIC PATIENTS. H Harris, R Scott, K
Gardner, J Lombard, FX Brennan, Genomind, Inc., Chalfont, PA, USA
INTRODUCTION: Underlying genetic differences may be an important factor in variable responses to
psychiatric medications. This study aimed to demonstrate the effectiveness of genetic testing using patient
and clinician reported outcomes, and to demonstrate the impact of genetic testing on clinician treatment
decisions. METHODS: This was a naturalistic unblinded, prospective analysis of the GeneceptTM Assay
(Genomind, Inc.). Study subjects included patients and their clinicians who ordered the saliva-based test.
Ten genes were tested: the serotonin transporter (SLC6A4), voltage-gated calcium channel (CACNA1C),
ankyrin G protein (ANK3), dopamine receptor subtype two (DRD2), catechol-O-methyl transferase (COMT),
methylene-tetrahydrofolate reductase (MTHFR), cytochromes P450 2D6 (CYP2D6), 2C19 (CYP2C19), and
3A4 (CYP3A4). An analytic results report was provided to the clinician and clinical support was available for
report interpretation. Clinicians were asked to complete a baseline survey which included assessment of the
patients medications, psychiatric history, and severity of illness using the Clinical Global ImpressionsSeverity (CGI-S) scale. A second survey was completed after results were received, capturing treatment
changes guided by the assay. The clinician assessed the patients improvement at 3 months using the
Clinical Global Impressions-Improvement (CGI-I) scale. Patients were asked to complete self-assessments
of depression, anxiety, medication side effects, and quality of life at three time points (baseline, 1 month,
and 3 months). RESULTS: Data related to 685 patients was collected. Approximately 70% had a mood
disorder and 29% an anxiety disorder. 73% of patients were treatment-resistant, having failed two or more
treatment trials prior to genetic testing. Clinician-reported CGI-I data indicated that 87% of patients showed
clinically measurable improvement post-Genecept. Strikingly, 91% of the treatment-resistant patients also
showed clinically measurable improvement. Patient reported data demonstrated significant increases in
quality of life, as well as decreases in depression, anxiety, and medication side effects; response rates
exceeded those reported in the seminal STAR*D trial at all levels. DISCUSSION: The data strongly support
the utility of pharmacogenetic testing in improving treatment outcomes in psychiatric patients. Response
rates were superior to those seen with standard treatment and were considerably higher than placebo
response rates. Response to psychiatric treatment is highly variable and often involves a number of failed
medication trials. Personalized therapies informed by genetic data can ease this burden and improve
patient outcomes. RESEARCH SUPPORT: This study was funded by Genomind, Inc (USA).
CRY OVER THE PAST OR STRESS ABOUT THE FUTURE? TIME PERSPECTIVES PREDICT PAIN
PERCEPTION AND CATASTROPHIZATION. B Gacs, A Csatho, University of Pecs, Medical School,
Department of Behavioral Sciences, Pecs, Hungary
INTRODUCTION: Pain is a pervasive stressor modulated strongly by emotional and cognitive factors. In
addition, there are a growing number of evidences that stress-coping strategies affect our experiences
with pain in many aspects. For example, pain catastrophization, the thoughts and feelings that individuals
29
experience in pain, has been found to modulate both pain perception, and pain responses. However,
similarly to other coping mechanisms, pain catastrophization might be plausibly influenced by how
individuals link their emotions and behavior to their life events in past, present, and future. More
particular, time perspectives, as the psychology dimension of time, has been shown to have a fundamental
influence on the interpretation of life events. Therefore, it is plausible to predict that pain catastrophization,
and pain perception are associated with time perspectives. In the current study, we tested this prediction by
using self-report instruments, and a picture-rating task. METHODS: Healthy participants (N = 150) were
asked to complete two questionnaires: The Zimbardo Time Perspective Inventory (ZTPI), and
the Pain Catasrophizing Scale (PCS). In addition, to measure pain perception, participants were presented
with 18 color pictures showing hands of people in various painful situations. On a scale ranging from 0 to
100, they were instructed to rate each picture in two aspects: Unpleasantness (perceived distress)
and Pain intensity. RESULTS AND DISCUSSION: The Rumination factor of PCS was found to be positively
associated with all factors of ZTPI, as well as with the Intensity and Unpleasantness ratings. Multiple linear
regression analysis, however, revealed that the Past-negative time perspective is the significantly
independent predictor of rumination. In addition, individuals with higher scores on the Past-negative
perspective indicated higher Unpleasantness ratings. Interestingly, future time perspective was associated
higher pain Intensity ratings. CONCLUSIONS: The results of the study provided clear evidences for the
association of pain catastrophization, and pain perception with time perspectives. The findings suggest that
individual differences in pain perception might partly arise from the different time-perspectives that
individuals develop when positive and negative life events are experienced.
SIMULTANEOUS EXPOSURE TO CHRONIC MILD STRESS AND ENRICHED DIETS ENHANCE
SPATIAL MEMORY VIA DIFFERENTIAL EXPRESSIONS OF DOPAMINE-4 AND METABOTROPIC
GLUTAMATE-5 RECEPTORS. PD Shallie, OF Shallie, AK Adefule, Department of Anatomy, Olabisi
Onabanjo University, Ago-Iwoye, Nigeria
INTRODUCTION: Stress has always been a major part of our lives, but it is especially prevalent in todays
society where we encounter a multitude of stressful situations on a daily basis. Although stress is usually a
manageable factor in our lives, it has been implicated in a wide range of disorders including disruption of
neuronal plasticity; fundamental mechanism of neuronal adaptation to external and internal stimuli. In this
study we explored how diets modulate the impacts of chronic mild stress on the spatial memory of Mice.
METHODS: Adult mice of both sexes were used, divided into two broad groups: Adult mice (equal number
of male and female) and prenatal mice. Each group was further subdivided into five subgroups: A (fed with
carbohydrate-enriched diet), B (fed with protein enriched diet), C (fed with high omega-3 enriched diet), D
(fed with antioxidants-enriched diet), E (fed with normal pellets). Each of the five subgroups consisted of
Stressed and Non-stressed cohorts. Exposure to stress and dieting commenced on day 9 of pregnancy until
the 50th postnatal day (PND 50), after which they were subjected to Barnes Maze to assess spatial
memory. The animals were sacrificed and the brains processed for qualitative analyses and
immunohistochemical staining for Dopamine (D4) and metabotropic glutamate 5 receptors (Glut5).
RESULTS AND DISCUSSION: Chronic mild stress and enriched diets significantly (p<0.05) reduced the
escape latency to 124 s, compared with 171 s in the non-stressed mice. Stress decreased the expressions
of mGlu5 at the dentate gyrus and CA3 regions of the hippocampus while increasing the expression in the
prefrontal cortex. The enriched diets increased the expression in the dentate gyrus (DG) and decreased the
expression in the prefrontal cortex (PFC) when compared to their respective controls. The expression is
higher at the DG and PFC of prenatal stressed fed with enriched diets than in the adult stressed mice. Our
findings showed that enriched diets and chronic mild stress improved spatial memory in the exposed mice
with sexual dimorphism.
EXAMINING THE RELATIONSHIP BETWEEN JOB STRESS, JOB SAFETY, AND JOB SATISFACTION.
SM Guy, Keiser University, Ft. Lauderdale, FL, USA
INTRODUCTION: Job stress has become a concern for many organizations as workplace injuries related to
job stress have increased significantly over the last few decades. Stress has been identified by employees
as one the most overlooked safety concerns of organizations. Moreover, an increase in job related stress
leads to many health concerns such as high blood pressure, heart attack, and anxiety and/or depression.
Furthermore, individuals who are highly-stressed are also more likely to engage in unsafe behaviors that
may lead to injury. METHODS: Participants were recruited using an online survey that was posted on social
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media. A convenience sample was collected via email and through a printed handout. Participants were
required to be at least 18 years old, currently employed, and working in the continental United States.
Stress was measured using the Job Stress Scale. Safety was measured using the Work Safety Scale, and
job satisfaction was measured using the Index of Organizational Reactions. The sample consisted of 115
participants. The majority of participants were Caucasian between 25-64 years of age. Males and females
were represented almost evenly. RESULTS AND DISCUSSION: Participants recruited through social
media did not differ from those recruited through the convenience sample, therefore the data was combined
in analysis. Multiple regression was conducted to identify the relationship between stress, organizational
commitment to safety, and job satisfaction. When job satisfaction was predicted, it was found that safety
(Beta = 0.56, p < 0.001) and stress (Beta = 0.34, p < 0.001) were significant predictors, F(2, 72) = 33.295, p
< 0.001. CONCLUSIONS: Perception of safety and stress were positively related to job satisfaction in both
high-risk and low-risk environments. This coincides with current research concerning stress, safety, and job
satisfaction. Moreover, job stress was negatively associated with job satisfaction in high-risk environments,
which also coincides with current research.
IMPLICATIONS OF HEALTH CARE COSTS ON CARDIOVASCULAR DISEASE PATIENTS: A
PSYCHONEUROIMMUNOLOGY PERSPECTIVE. I Barreiro, M Corneille, S Behbahani, ClinPharm, Albizu
Universitry, Doral, FL, USA
INTRODUCTION: Given the negative impact of psychosocial factors including the financial burden of
cardiovascular disease, the leading cause of mortality and morbidity worldwide, providing quality of care
and lower health care costs has become challenging. Furthermore, well-supported findings have
established a bi-directional relationship between psychological distress (i.e., depression) and cardiovascular
health, further evidenced by the interaction between serotonin levels and platelet activation among
cardiovascular disease patients. Therefore, increased costs and limited level of access to quality health
care leads to increased stress for cardiovascular patients, which is associated with worsening of
cardiovascular disease. METHODS: An exhaustive review of the literature was conducted through the
following search engines: PubMed, EBSCO, NCBI, and PsycINFO. Researchers utilized the following
keywords resulting in the selection of articles: psychological factors and cardiovascular disease, health care
costs, affordable care act and prevention of chronic diseases, and depression among cardiovascular
disease patients among others. Articles selected to be reviewed span from 2010 to 2015. Overall, from a
psychoneuroimmunology perspective, this review seeks to identify quality and costs of health care among
cardiovascular disease patients as a moderator of disease progression. RESULTS AND DISCUSSION:
Implementation of the Affordable Care Act (ACA) has increased accessibility to quality health care and
preventative measures, which may reduce the onset, progression, and costs of treatment associated with
cardiovascular disease. In other words, the ACA proposes a primary versus tertiary model of care. In
addition, the ACA has promoted further development of an interdisciplinary care model within primary care
settings, which has been evidenced as an effective treatment strategy for multiple cardiovascular diseases
(i.e., hypertension). An extensive review of the literature further supported the psychoneuroimmunology
explanation for cardiovascular disease and implementation of the ACA as a moderating factor of such
interaction. RESEARCH SUPPORT: Health Care Cost Institute 2013 Health Care Cost and Utilization
Report (www.healthcostinstitute.org/2013-health-care-cost-and-utilization-report).
EXAMINING THE ROLE OF COMMUNICATION AND SOCIAL SUPPORT AS A MEDIATOR OF STRESS
RESILIENCE AMONG CLINICAL PSYCHOLOGY DOCTORAL STUDENTS. JC Lyons, SR Webster, CA
Feldman, B Nierenberg, Center for Psychological Studies, Nova Southeastern University, Ft. Lauderdale,
USA
INTRODUCTION: Graduate students are under large amounts of stress, however little is known about the
influence of social support on stress resilience. The main goal of the current study is to assess the
relationship between social connectedness and method of communication among graduate students. In
doing so, this study will assessed how these variables relate to stress resilience. This study sought to
understand the role that interpersonal relationships, fostered by face-to-face contact plays in stress
resilience among doctoral students (Cahir and Morris, 1991; Hodgson and Simoni, 1995). It was, therefore,
hypothesized that graduate students with more face-to-face contact would report better overall well-being in
compared to those with less face-to-face contact. METHODS: Data was collected from doctoral students (N
= 45; 20% males, 80% females, M age = 26) enrolled in two private universities in the US. Participants
31
completed an online survey with items addressing the frequency and preference of different forms of
communication. Self-report measures were completed: Graduate Stress Inventory-Revised, Perceived
Stress
Scale,
and
the
Inventory
of
Socially
Supportive
Behaviors-Short
Form.
RESULTS AND DISCUSSION: A one-way ANOVA revealed that there was no significant relationship
between mean scores on the GSI-R and type of communication preferred F (4,35) = 0.701, p = 0.596.
Frequency counts were utilized to assess the hierarchal relationship between types of preferred
communication: text messaging (59.6%), face-to-face (21.3%), phone call (13.0%), email (2.1%) and social
media (2.1%). Frequency counts were also conducted to assess if stress was a moderator on the type of
communication preferred: face-to-face (61.7%), phone call (17.0%), text messaging (10.6%), social media
(2.1%) and other (6.4%). Findings revealed that participants ranked Sometimes to Fairly Often experiencing
the following: control irritations in your life (66%), unable to control important things in life (63.8%), not able
to cope with all the things that you have to do (44.7%), and felt nervous and stressed (29.8%). The
demands of a doctoral program may require a substantial amount of social support to counter and cope with
associated stressors. Social support is defined as the frequency of social interaction that fluctuates during
stressful times (Goplerud, 1980). Psychology doctoral programs are stressful and the day-to-day demands
and pressure to apply for internship may be especially draining and tests students perseverance (Saunders
and Balinsky, 1993; Stecker, 2004). Students may require a substantial amount of social support to cope
with these stressors. Social support is the frequency of social interaction that fluctuates during stressful
times (Goplerud, 1980). Individuals who are more socially isolated reported greater cumulative stress than
socially supported individuals. As a result, less connected individuals may experience more damage to their
mental and physical health. Further, Kobasa and Puccetti (1983) asserted that given social resources
individuals experience more feelings of closeness and identification with others. Much of the research on
communication focuses solely on the effects of cell phone use and social media, but discounts
understanding the effects of face-to-face contact in regards to communication methods. Borae and Park
(2010) examined the relationship between face-to-face communication and cell phone with feelings of
loneliness. Results revealed that individuals who engaged in more face-to-face interactions also engaged in
more cell phone use, which in turn lead to lower scores on the loneliness scale. Those who scored higher
on the loneliness scale had lower face-to-face interactions and used their cell phones less. The researchers
suggested that those who report higher levels of loneliness find less pleasure and interpersonal gain by
using their cell phone, as they are also engaging in less face-to-face interaction. They also found that the
motive for using cell phones was for such things as showing affection, which is related to face-to-face
interactions (Borae and Park, 2010). Kim et al. (2009) asserted that individuals who engaged in compulsive
Internet use did so to overcome feelings of depression and loneliness. Further, individuals who used the
Internet to foster social connectedness may have been unintentionally perpetuating their feelings of
loneliness, as online activities limited them from engaging in face-to-face contact. Based on these results, it
is important that future research examines how face-to-face interaction plays a role in mental health
resiliency. Those in the service of helping others, such as individuals trained in the field of psychology, tend
to experience burnout from lack of self-care. Therefore, we will be examining how the communication
patterns of these students in a high-stress and high-demand graduate program are impacted. We hope to
identify the most effective communication strategies for these graduate students. The results may assist in
future stress reduction and adjustment to future life stressors. In addition to the reasons stated above, this
study may provide information and incentives that will help motivate universities to create organizations
and/or provide support to the students who need help coping with student life stressors.
PERCEIVED DISCRIMINATION, ACCULTURATIVE STRESS, AND DEPRESSIVE SYMPTOMS IN

MEXICAN AMERICANS: FAMILISM AND ETHNIC IDENTITY AS MODERATORS. H-L Cheng, JL Rislin,
X Nguyen, C Williams, CH Cha, B Stamper, R Zamora, GA Liu, J Peters, New Mexico State University, Las
Cruces, NM, USA
INTRODUCTION: Hispanics have become the largest ethnic minority group in the US, among which
Mexican Americans are the largest Hispanic subgroup (Rumbaut, 2006). Given their historical and recent
sociocultural contexts related to immigration, many Hispanics face substantial ethnic-minority stressors,
such as educational and occupational barriers, blatant and subtle ethnic discrimination, and burdens of
acculturative stress (Brondolo et al., 2005; Gallo et al., 2009). Ethnic discrimination and acculturative stress
have been linked to negative mental health outcomes, including increased depressive symptoms among
Hispanics (Driscoll and Torres, 2013; Torres and Ong, 2010). This study focuses on the impact of
acculturative stress and perceived discrimination on depression, a biobehavioral mental health concern, for
32
Latino individuals. Factors Familism and Ethnic Identity were assessed to determine whether they help to
attenuate or strengthen the connection between acculturative stress, perceived discrimination, and
depressive symptoms. METHODS: A total of 211 Mexican Americans from a mid-sized university in the
Southwest were recruited for this study (Mean age = 26.72 years, SD = 9.32). The sample consisted of
significantly more women (150) than men (61). In terms of generation status in the US, most participants
identified as second generation (44.5%), followed by first generation (16.6%), fourth generation (13.7%),
fifth generation (13.7%), and third generation (11.4%). Measures: Riverside Acculturation Stress Inventory
(Benet-Mart nez and Haritatos, 2005), General Ethnic Discrimination Scale (Landrine et al., 2006),
Attitudinal Familism Scale (Steidel and Contreras, 2003), Multigroup EthnicIdentity Measure (Robertsetal,
1999) Patient Health Questionnaire-9 (Kroenke et al., 2001) for depression. RESULTS DISCUSSION: A
total of four hierarchical multiple regression analyses were performed with acculturative stress and
perceived discrimination as respective independent variables, and gender, familism and ethnic identity as
respective moderators, for the prediction of depressive symptoms. The Bonferroni correction was applied (
= 0.05/4 = 0.0125) to account for the number of regression analyses conducted. Results indicated a
significant two-way interaction between familism and acculturative stress ( = -0.25, p < 0.01) such that
familism attenuated the association between acculturative stress and depressive symptoms. In addition, a
three-way interaction between gender (0 = women, 1 = men), ethnic identity, and acculturative stress was
found ( = 0.25, p < 0.01): (a) at higher levels (+1SD) of ethnic identity, the association between
acculturative stress and depressive symptoms became non-significant (i.e., not different from zero) for
women (b = 1.12, = 0.16, p = 0.14), but the association remained significant for men (b = 4.79, = .68, p
= 0.00); (b) At lower levels (-1SD) of ethnic identity, however, the association between acculturative stress
and depressive symptoms was not significant for men (b = 1.39, = 0.20, p = 0.31), but this association
was significant for women (b = 3.48, = 0.49, p = 0.00).
ULTRASONIC VOCALIZATION DURING EXPLORATION IN BOTH SAFE AND STRESSFUL
ENVIRONMENT IN ADULT MALE MICE. H-S Mun, JC Roder, T Lipina, Lunenfeld-Tanenbaum Research
Institute, Mount Sinai Hospital, Department of Molecular Genetics, University of Toronto, Toronto, ON,
Canada
INTRODUCTION: Approach and avoidance conflict exists during exploration in novel open fields. The open
field is frequently utilized in genetically modified strains in order to get insights of emotionality and anxiety.
However, we recently showed that exploration in the open field under dim light could also encompass
cognitive and motivational features. Rats emit 50-kHz ultrasonic vocalizations (USVs) associated with
approach behavior, while emitting 22-kHz alarm calls in the presence of stress. But in mice, it remains
unknown if call types are differently used depending on the context and if they are correlated with
exploratory and avoidance states. METHODS: We recorded the calls of adult C57BL/6J male mice in
various behavioral conditions, such as novel and familiar environments. We also introduced a modulator for
anxiety, by comparing lighting levels such as dim light and bright light. RESULTS AND DISCUSSION: Male
mice uttered USVs in all novel and familiar situations, and even in a bright context. Nevertheless, they
emitted the highest number of calls in novel dim, and least amount of calls in familiar environments. For
mice explored in the novel dim environment, at the time of high frequency calls, mice mostly showed
unsupported rearings. And for mice explored in the novel bright environment, at the time of low frequency
calls, mice reared to the walls as an avoidance behavior. These results open the way for a deeper
understanding and characterization of acoustic signals associated with exploratory behavior. They can also
help evaluate the role of the anxiety state in the emission of acoustic signals. RESEARCH SUPPORT: This
study was supported by the Canadian Institutes of Health Research (CIHR, MOP111198).
EXAMINING BEHAVIORAL DIFFERENCES BETWEEN WILD-CAUGHT AND DOMESTICATED

(LABORATORY) ZEBRAFISH, DANIO RERIO. AV Kalueff, ISBS Fellow, S Homechaudhuri, C Song, S Li,
Y Liu, A Mitra, S Pal, A Chaudhuri, A Roy, M Biswas, D Roy, A Podder, A Kaluyeva, P Chen, L Yang, JJ
Wang, AM Stewart, ISBS Fellow, Research Institute for Marine Drugs and Nutrients, College for Food
Science and Technology, Guangdong Ocean University, Zhanjiang, China; Department of Zoology,
University of Calcutta, Kolkata, India; ZENEREI Institute and the International Zebrafish Neuroscience
Research Consortium (ZNRC), New Orleans, USA; Institute for Translational Biomedicine, St. Petersburg
State University, St. Petersburg, Russia
33
Zebrafish are emerging as an important model organism for neurobehavioral and neurophenomics
research. The majority of zebrafish behavioral assays are performed using inbred or outbred laboratory
(domesticated) zebrafish. Given the importance of genetic and environmental (natural habitat-related)
influences on zebrafish behavior, it is important to examine potential behavioral differences between
domesticated and wild-derived zebrafish. Here, we characterize wild-derived adult shortfin zebrafish
(collected from rivers in North Bengal, and quarantined for several months in a farm near Kolkata, India)
and adult laboratory outbred short-fin zebrafish obtained from a commercial breeder in Zhanjiang
(Guangdong, China). Behavioral testing of zebrafish was performed 1 day after zebrafish delivery, and was
done in parallel, respectively, in the Aquatic Bioresource Research Laboratory, University of Calcutta (India)
and Research Institute for Marine Drugs and Nutrients (RIMDN), College for Food Science and
Technology, Guangdong Ocean University (China). Utilizing the standard 5-min novel tank paradigm,
we assessed anxiety-related (top/bottom preference, freezing, erratic movements) generated using manual
observation. In addition, we also compared locomotor parameters (e.g., distance traveled, velocity, turning)
in both groups using automated registration and analyses (Ethovision XT8, Noldus IT, Netherlands).
Overall, we found marked differences between the groups in anxiety level (wild-derived >>> laboratory
zebrafish) and altered within-session habituation (impaired per-minute patterning of behavior in wild fish),
suggesting potential strong impact of genetic differences between wild-caught and laboratory-derived fish
on their affective/emotional behavior and cognitive phenotypes. Our study further establishes how zebrafish
behaviors are modulated by various experimental factors, and suggests that various strains can be used as
tools for zebrafish neurobehavioral and neurophenomics research.
Day 3. Wed, June 24, 2015
Morning session
9th INTERNATIONAL ZEBRAFISH NEUROSCIENCE SYMPOSIUM AND WORKSHOP
Chairs: AV Kalueff, ISBS Fellow (USA), DJ Echevarria, ISBS Fellow (USA)
ZNRC LECTURE: ZEBRAFISH BIOLOGICAL PSYCHIATRY. DJ Echevarria, ISBS Fellow, Department of
Psychology, University of Southern Mississippi, Hattiesburg, MS, USA
The zebrafish (Danio rerio) is emerging as a new important species for studying mechanisms of brain
function and dysfunction. Here, I will review several key CNS disorders which can benefit from developing
and applying novel zebrafish models.
ZNRC PRESENTATION: FROM A TO Z: ACTIVITY ANALYSIS AND DETAILED OBSERVATIONS IN
ZEBRAFISH. J Rogers, Noldus Information Technology Inc., Leesburg, VA, USA
INTRODUCTION: As their physiology continues to become more and more elucidated, zebrafish, or Danio
rerio, are increasingly being used as a model organism for their importance in the field and their relative
low-cost maintenance. Some researchers have even made the claim that zebrafish are 'the ideal organism'
for studying neurogenetics. Furthermore, many federal granting agencies consistently rank the zebrafish as
one of the most important experimental organisms. Yet behavioral and physiological analysis of zebrafish
disease models, genetics, pharmacology, toxicology, and neuroscience require tools that combine
accuracy, reliability, and validity with ability to perform high throughput analyses. Automated recording, data
collection, and analysis using the tracking systems presented here increase efficiency, standardization, and
understanding into human-related disorders. METHODS: For studies of embryos and larvae, we developed
a comprehensive system by combining the latest in video tracking, image processing, and pattern
recognition with high-end optics in a completely controlled environment. The transparency of zebrafish
embryos and larvae also lends perfectly to the study of activity and tail coiling of embryos, as well as
psychoneuroimmunology functioning in larvae. In addition, individual zebrafish tracking has led to many
new insights and discoveries into stress, anxiety, and translational markers for biological psychiatry. Beyond
34
individual behavior and physiology, adult zebrafish are an important model of complex social phenotypes as
they engage in shoaling behavior that is highly sensitive to experimental manipulations. Using our software
to track an entire shoal, several neuropsychopharmacological studies have demonstrated significant effects
on group size and shoaling activity. The availability of such tools for zebrafish research will further enhance
experimental modeling of the general psychiatry of stress-related brain disorders. Three-dimensional
analysis of adult zebrafish swimming paths offers another promising methodology. Compared to traditional
two-dimensional traces, three-dimensional swim path reconstructions enable many novel approaches to
analyzing behaviors related to stress, memory, and learning. These three-dimensional track analyses offer
movement parameters within 3D space, which opens up many possibilities into the neurogenetics,
neurochemistry, and neurophysiology of stress. RESULTS AND DISCUSSION: The various techniques
presented here offer scientists valuable high-throughput quantitative methodologies for screening
physiological and behavioral profiles of disease, illness, toxicology, genetics, and pharmaceutical
compounds.
ZNRC LECTURE: MODELING ACUTE AND CHRONIC STRESS IN ADULT ZEBRAFISH AN UPDATE.
AV Kalueff, ISBS Fellow, S Li, Y Liu, P Chen, L Yang, JJ Wang, A Kaluyeva, AM Stewart, ISBS Fellow, C
Song, Research Institute for Marine Drugs and Nutrients (RIMDN) and College of Food Science and
Technology, Guangdong Ocean University, Zhanjiang, China; ZENEREI Institute and the International
Zebrafish Neuroscience Research Consortium (ZNRC), New Orleans, USA; Dalhousie University, Halifax,
Canada; Institute for Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia
Zebrafish (Danio rerio) is an important novel model organism emerging as a new important species for
studying a wide range of stress-related brain disorders. Together with robust behavioral responses to stress
observed in adult zebrafish models, well-developed HPI stress neuroendocrine axis and excellent cognitive
capability make this aquatic species a particularly useful models to study affective brain disorders, such as
anxiety, PTSD and depression. In addition to well-documented behavioral and physiological responses to
acute stress, mounting evidence indicates high sensitivity of zebrafish to anxiolytic and anxiogenic
pharmacological modulation. As their developing utility to model acute models relevant to anxiety, fear and
panic continues to unravel, the value of zebrafish for modeling various aspects of chronic, long-term stress
also becomes clear. For example, applying a long-term battery of unprediclable stressors, we have
established a model of zebrafish anxious depression-like pathogenesis, accompanied by increased stresslike behaviors, elevated cortisol, and altered dendritic spines (suggesting affected synaptic plasticity) in
stressed fish vs. non-stressed controls as well as vs. stressed cohorts treated with chronic fluoxetine.
Outlining the role of zebrafish in modeling human emotional disorders, this talk will discuss recent
applications and existing challenges in this field, as well as outline the potential of zebrafish models in
behavioral phenomics and high-throughput genetic/small molecule screening for anxiolytic and
antidepressant drug discovery.
Afternoon session
ZNRC PRESENTATION: VIEWPOINT. P-A Fel, ViewPoint, Montreal, Canada
ZebraLab is a state-of-the-art automated observation and video tracking solution designed by ViewPoint. It
is a complete system for high-throughput tracking and behavioral analysis of zebrafish (Danio rerio).
ZebraLab can be deployed to track zebrafish larvae in multi-well plates, as well as single adult zebrafish,
and shoals. Our new feature: Fast Data Monitor. Because of the ever-increasing precision of the Viewpoint
behavior analysis systems, the amount of data provided naturally grows. Fast Data Monitor is the answer to
address data management. Its advantages include: User-friendly interface; No specific language to learn;
Keeps templates to reuse with your experiments.
ASSESSING THE EFFECTS OF LIGHT DARK MANIPULATION AND CAFFEINE EXPOSURE ON
ZEBRAFISH SLEEP BEHAVIOR. KM Khan, DJ Echevarria, ISBS Fellow, NR Lodinger, AD Collier,
Department of Psychology, University of Southern Mississippi, Hattiesburg, MS, USA
Zebrafish are a well-known and widely used model organism in behavioral research; several
neurotransmitter systems and neuropeptides are conserved in this species, allowing for a comparative
35
understanding of sleep and sleep structures. Previous study has revealed changes in gene expression
following an exposure to constant light and constant dark conditions. Here, we expand on the literature and
work to build upon the current understanding of sleep behavior in this small teleost fish. We assessed sleep
and wake behavior within groups of three fish to alleviate the stress effects of being removed from the
shoal. Sleep and wake behavior of the groups were analyzed following three sleep-wake cycle
manipulations: (1) constant light conditions, (2) constant dark conditions, and (3) constant light conditions,
paired with the administration of an adenosine antagonist. Following sleep-wake cycle manipulations,
animals were transferred to an additional apparatus for either novel tank testing or cortisol measurements.
Antagonism of adenosine during prolonged light exposure produces both behavioral and physiological
changes. Cortisol expression was unchanged in animals exposed to constant light conditions, and was
elevated in animals exposed to both caffeine and constant light. Animals in the constant light condition
tended not to explore regions of their environment when placed in a novel tank; they also tended to move
erratically, and dwell in the lower region, when compared to animals in other light/dark manipulation
conditions.
DISC1 MODULATES THE STRESS RESPONSE IN LARVAL ZEBRAFISH VIA HYPOTHALAMIC
GENES. HL Eachus, J Wood, M Placzek, PJ Watt, Department of Animal and Plant Science, Bateson
Centre, Department of Biomedical Science, Sheffield Institute for Translational Neuroscience, Department
of Neuroscience, University of Sheffield, Sheffield, UK
INTRODUCTION: Stressful experiences trigger responses, which allow us to undergo adaptive changes. In
vertebrates, these critical changes to physiology, metabolism and behavior are mediated by the
hypothalamic-pituitary-adrenal (HPA) circuit, which acts to maintain homeostasis. Excess stress during
critical periods of development affects brain maturation, which in humans has been linked to the
development of adult-onset neuropsychiatric disorders. Evidence from animal models has demonstrated
that stress in early life can have effects on behavior, endocrine function and gene expression, which may be
mediated by epigenetic imprints. In addition there is a growing literature suggesting that interplay between
genetic and environmental factors (GXE) contributes to individual differences in susceptibility to psychiatric
illness. METHODS: Zebrafish are becoming an increasingly popular model in behavioral neuroscience and
lend themselves to high-throughput behavior-based screens. This work utilizes a zebrafish GXE model and
investigates the role for an orthologue of a genetic risk factor for psychiatric illness in humans (Disrupted-InSchizophrenia 1) in the stress response. Larval zebrafish were exposed to an acute osmotic stressor,
during which their swimming behavior was monitored and subsequent changes in endocrine function were
analysed. The expression patterns of stress-related genes were also assessed in disc1 zebrafish.
RESULTS AND DISCUSSION: Our data suggest that disc1 mutant zebrafish display abnormal endocrine
and behavioral responses to a chemical stressor. We also observed altered expression of genes linked to
the HPA axis in the hypothalamus of developing disc1 mutant larvae. This evidence suggests that disc1
alters hypothalamic gene expression during development, which subsequently affects behavioral and
endocrine responses to stress. RESEARCH SUPPORT: This research was supported by The University of
Sheffield
THE EFFECTS OF METHYLENE BLUE ON LEARNING ACQUISITION IN THE ZEBRAFISH. EM
Caramillo, DJ Echevarria, ISBS Fellow, Department of Psychology, University of Southern Mississippi,
Hattiesburg, MS, USA
The utility of the zebrafish in learning acquisition research has been documented with promising results
over the past few decades. Zebrafish exhibit learning processes comparable to those of their mammalian
counterparts. In order to test for such acquisition, a wide variety of tasks have been created and/or adapted
from the rodent literature. For instance, the t-maze has been used with great success in rodent research
and has begun to be used with zebrafish to demonstrate the animals learning abilities. By demonstrating
such abilities in the zebrafish using the t-maze, we ask: how does the zebrafish respond to known cognitive
enhancing substances such as the histological stain methylene blue? In small doses, methylene blue has
been shown to have cognitive enhancing effects in rodents. While such an effect has been shown in other
animals, it has yet to be tested in the zebrafish population. By testing for such outcomes, we hope to exhibit
another instance in which the zebrafish can be used to study human responses as well as bolster the
viability of using the cost effective, easy-to-care-for species in replacement of other model organisms.
36
ADULT ZEBRAFISH AND CONDITIONED PLACE PREFERENCE AS A MODEL OF DRUG REWARD.

AD Collier, NR Lodinger, KM Khan, EM Caramillo, DJ Echevarria, ISBS Fellow, Department of Psychology,
University of Southern Mississippi, Hattiesburg, MS, USA
Addiction and substance abuse amass hundreds of billions of dollars annually in costs associated with
healthcare, crime and lost productivity, solely within the United States. Sanctioned drugs, such as alcohol,
nicotine and caffeine, are the most frequently used psychoactive substances, all of which have potential to
induce psychological and/or physiological dependence. Efficacious treatments remain few in number, the
development of which will be facilitated by comprehension of environmental, genetic, pharmacological and
neurobiological mechanisms implicated in the pathogenesis of addiction. Animal models such as the
zebrafish (Danio rerio) have gained momentum within various domains of translational research, and as a
model of complex brain disorders (e.g., addiction). Behavioral quantification within the conditioned place
preference (CPP) paradigm serves as a measure of the rewarding qualities of a given substance. If animals
develop an increase in preference for the drug-paired environment, it is inferred that the drug has positivereinforcing properties. The current study reports CPP behavior in zebrafish following acute (1 day) or
chronic (7 days) exposure to alcohol (0.0%, 0.25%, 0.50% or 1.00% vol/vol), caffeine (0 mg/l, 50 mg/l, 100
mg/l and 150 mg/l) and nicotine (0 mg/L, 2.5 mg/l, 5 mg/l and 10 mg/l).
ABSTRACT COMMUNICATIONS:
PRENATAL EXPOSURE TO STRESS AND MORPHINE MODIFIES CORTICOSTERONE BLOOD
LEVELS AND PENTYLENTETRAZOLE (PTZ)-INDUCED EPILEPTIC BEHAVIORS IN RAT PUPS. E
Saboory, E Nakhjiri, S Roshan-Milani, Neurophysiology Research Center, Department of Physiology,
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran
INTRODUCTION: Stressful events during gestation have important effects on the later physical and mental
health of the offspring. The present study aimed to identify effects of co-administration of stress and
morphine in prenatal period and re-exposure to stress at the end of infancy on corticosterone blood levels
and pentylentetrazole (PTZ)-induced epileptic behaviors in rat. METHODS: Pregnant rats were divided to
six groups of control, stress, saline, morphine, stress-saline and stress-morphine. In the stressed group,
rats were stressed and held immobile for three consecutive days started on day 15 of pregnancy. The rats
in saline and morphine groups received saline and morphine in the same days. In the morphine/salinestress groups, rats were exposed to stress and received morphine/saline simultaneously. On postnatal day
22, half of the pups re-exposed to stress, and PTZ-induced epileptic behaviors of each rat were assessed.
Blood samples were collected to determine corticosterone level. RESULTS AND DISCUSSION: Latency of
first epileptic behavior decreased significantly in stress-morphine group compared to other groups. Reexposure to stress significantly decreased the number of clonic seizures. There was no significant
difference between the experimental groups in duration of clonic attacks and mortality rate. The level of
corticosterone showed a significant increase in stressed pups, but decreased in the morphine group. In
conclusion, these results indicated that co-administration of morphine and restraint stress during late
pregnancy had profound impact on neurochemical development and might alter vulnerability to PTZinduced epileptic behaviors. Prenatal stress is more powerful than postnatal stress on influencing neural
development and seizure susceptibility in rats. RESEARCH SUPPORT: This research was supported by
the Research Council of Urmia University of Medical Sciences, Urmia, Iran.
ADOLECENT EXPERIENCES WITH STRESS IN DISPLACED PERSONS CAMPS FIVE YEARS AFTER
THE EARTHQUAKE OF HAITI. S Lorwinsky, C Pierre, Institute of Social Work and Social Science of Scott
(ETSS), Port-au-Prince, Haiti
There is a consensus among researchers that agree that stress is pervasive and serious problem for young
people across cultural contexts. It has a negative effect on a childs life, diminishing both health and
academic performance. Perception plays a large role in experiences with stress and a young persons
ability to develop stress-management strategies is instrumental for coping.
The purpose of this study is to understand how young Haitian Internally Displaced Persons experience
stress five years after the January 2015 earthquake. We seek to better understand the evolution of quake-
37
related stress for young displaced Haitians and discover how experiences with stress have changed over
time. We demonstrate that even five years after the quake, young victims who are living in the camps still
experience high levels of daily anxiety and trauma. The literature on childhood PTSD and trauma is
saturated with post-disaster assessments, so this study will be particularly important because it looks at
experiences with stress five years post-disaster (which we know very little about). To evaluate this issue, we
conduct semi-structured qualitative interviews with sixty young people who are living in Haitian IDP camps,
aged 10 to 16. Furthermore, we interview both parents about the behavior of their children at home and
teachers about the behavior of children in school. We find that the majority of young people living in IDP
camps experience have persistent stress and have earthquake-related trauma. They describe their lives as
unsafe, miserable, and poverty-ridden. Additionally, they experience problems with both school
performance and health. They have difficulties studying, participating in activities, and engaging in stress
management. Our study is a rich qualitative study that includes the voices of young people who often find
no public voice and we look forward to sharing our findings with fellow researchers. RESEARCH
SUPPORT: This research was supported by Institute of Social Work and Social Science (ETS).
GENETIC BASIS OF PARKINSONS DISEASE: AN INDIAN SCENARIO. J Ray, SN Pradhan Centre for
Neurosciences, University of Calcutta, Kolkata, India
BACKGROUND: Parkinsons disease (PD) is an etiologically complex neurodegenerative movement
disorder, which affects 2% of the population over 65 years; only ~10% are familial cases. Till date 20
chromosomal loci and 14 causal genes have been reported. Besides candidate genes, several modifier
genes have been found to be associated with altered disease risk. Purpose: To identify genetic variants,
either causal or associated, posing risk for PD among Indian cases. Methods: Nucleotide variants were
identified by PCR-sequencing of exons along with the flanking splice junctions. Gene dosage mutations
were identified by MLPA technique. RESULTS: Candidate gene studies revealed that Parkin mutations are
most common and with different ethnic background the frequency of mutations varied from 1.96-10.56%.
Two Parkin variants, Gln34Arg & Arg334Cys, were found to be most prevalent among Indian PD patients.
Haplotype analysis suggested that the recurrence of these mutations did not result from founder effect.
Studies from East India revealed mutations in PINK1 & DJ-1 represent 2% & 4% of PD cases respectively.
Till date, while a single mutation has been reported in LRRK2 in Indian patients none found in Alphasynuclein. Several modifier genes found to influence sporadic PD among Indians. Association studies using
SNPs from various genes including MAPT, GSK-3, DAT, DBH, FGF-20, APOE, BDNF were found to act
as risk or protective factors. Some alleles influenced age of onset. CONCLUSION: Studies from different
laboratories including ours demonstrate that genetic factors play a significant role for the Parkinsons
disease pathogenesis among Indians. RESEARCH SUPPORT: This study was supported by CSIR, ICMR,
UGC, DST-PURSE and DST-CSI, Government of India
EMBRACING THE NEW WITH AN ESTABLISHED METHODOLOGY: NAVIGATING THE APPROACH
WITH STRESS AND BEHAVIOR STUDIES. W Bowden, S Finnegan, Biorasi, Miami, FL, USA
The Stress and Behavior research arena presents unique challenges fuelling the development of innovative
methods to combat this complicated space. Subjects who have grown accustomed to their current
treatment may not be open to a changed drug curriculum even if the new curriculum could yield better
results. This unfortunate circumstance Is prevalent when dealing with patients who are going from
medication to other forms of treatment. As research and methods in this area evolve, patients are being
offered more options which do not always mandate being medicated. However, objective testing is needed
to ascertain efficacy. As a CRO, Biorasi (www.biorasi.com) is on the side of research supporting innovative
solutions such as increased individualized attention to encourage compliance and easy to use technology.
Efficient systems make it easy for subjects to check in with study personnel. Another major component of
any research study and one which certainly comes into play in stress and behavior studies is recruitment
and enrollment. It is important to have strong relationships with principal investigators (PIs) to encourage
them to identify and enroll subjects from within their patient base. However, this too requires increased
emphasis when possibly encouraging patients to forego their current treatment. The CROs job is to impress
upon PIs the importance of their knowledge of patients conditions, treatment and openness to alternate
treatments to ascertain willingness to enroll and comply once the study begins. Outside of PI engagement,
recruitment relies on involvement with the community and advocacy groups. Biorasi is a creative and
committed global CRO positioned to answer the challenges of unorthodox research studies.
38
THE INTERNATIONAL STRESS

AND BEHAVIOR SOCIETY (ISBS)
Established in 2007
ISBS is the international society of experts working with a wide range of topics in the field of translational
neuroscience, neurobehavioral sciences, biopsychology and biopsychiatry, with a particular focus on stress,
stress-related neurobehavioral phenotypes, their neural, molecular and genetic mechanisms, as well as
stress-evoked neuropsychiatric disorders.
Anyone with an interest in stress-related human or animal behaviors, neurobehavioral disorders and their
mechanisms, wishing to join ISBS, can do so by paying dues. Payment can be made following sending the
e-mail form and payment request to the ISBS Secretariat at info@stressandbehavior.com. Once the form
and the payment have been received, you will receive a membership confirmation.
Membership:
Regular membership dues are $100.00 for the period of three years, or $60.00 for the period of one year.
Student (undergraduate and graduate) membership dues are $60.00 for the period of three years.
Regular membership benefits include a $50.00 discount for registration for any of the ISBS
Conferences, symposia, workshops and summer schools.
Student members will benefit from a $25.00 discount for registration for any of the ISBS
Conferences, symposia, workshops and summer schools.
Membership cycle starts January 1st. ISBS Members benefit from reduced STRESS, BRAIN &
BEHAVIOR journal subscription fees: $70.00 (regular member), $55.00 (student member).
ISBS Membership application form (please fill in and send by e-mail to the ISBS Secretariat at
info@stressandbehavior.com, with the subject ISBS Membership request)
Name, Family name:

Position/Title:
Category - please select one:
Regular member, 3-year term ($ 100.00)
Regular member, 1-year term ($ 60.00)
Student member, 3-year term ($ 60.00)
Address (affiliation):
Postal code:
State:
Country:
Phone, Fax:
E-mail address:
www:
Institute/Company:
City:
www.stress-and-behavior.com
info@stressandbehavior.com
39
Fellows of ISBS:
The ISBS Fellowship (with Life membership) is the highest honor bestowed by the International Stress
and Behavior Society. It is awarded annually to international scholars, in recognition of their
contribution to clinical or translational neuroscience, biological psychiatry and stress physiology
research and/or education, as well as for their long-standing support of the ISBS mission and its
national, regional or international programs.
Dr. Mikhail Aghajanov (Yerevan Medical University, Armenia), 2015
Dr. Elliott Beaton (University of New Orleans, USA), 2015
Dr. Evgeniy Budygin (Wake Forest Medical Center, USA), 2014
Dr. David Echevarria (University of Southern Mississippi, USA), 2014
Dr. Alexey Egorov (Sechenov Institute, Russia), 2014
Dr. Irina Ekimova (Sechenov Institute, Russia), 2013
Dr. Raul Gainetdinov (Italian Institute of Technology, Italy), 2013
Dr. Allan Kalueff (ZENEREI Institute, USA), ISBS President, 2013
Dr. Victor Klimenko (Institute of Experimental Medicine, Russia), Vice-President, 2013
Dr. Mamiko Koshiba (Tokyo University of Agriculture and Technology, Japan), 2014
Dr. Shun Nakamura (Tokyo University of Agriculture and Technology, Japan), 2014
Dr. Tatyana Nevidimova (National Mental Health Institute, Russia), 2014
Dr. Yuriy Pastuhov (Sechenov Institute, Russia), 2013
Dr. Mikhail Pletnikov (Johns Hopkins University, USA), 2015
Dr. Tatyana Sollertinskaya (Sechenov Institute, Russia), 2013
Dr. Adam Stewart (ZENEREI Institute, USA), 2015
Dr. Tatyana Strekalova (Maastricht University, Netherlands), 2014
Dr. Gilbertha St. Rose (Eden Herbs, St. Lucia), 2015
Dr. Oleg Syropiatov (UAPO, Ukraine), 2013
Dr. Sergei Tsikunov (Institute of Experimental Medicine, Russia), 2014
Dr. Jason Warnick (Arkansas Tech University, USA), 2014
ISBS Fellow Nominees:

Dr. Louis Newman (Destiny Medical School, St. Lucia), 2016
Dr. Urban Seraphin (Allied Health Council, St. Lucia), 2016
Dr. Dusko Kozic (University of Novi Sad, Serbia), 2016
40
THE INTERNATIONAL STRESS AND

BEHAVIOR SOCIETY (ISBS)
Established in 2007
ISBS is the international society of experts working with in the field of clinical and translational neuroscience,
neurobehavioral sciences, biopsychology and biopsychiatry, with a particular focus on stress, stress-related
neurobehavioral phenotypes, their neural, molecular and genetic mechanisms, as well as stress-evoked
neuropsychiatric disorders. Anyone with an interest in stress-related human or animal behaviors, neurobehavioral
disorders and their mechanisms, wishing to join the International Stress and Behavior Society can do so by paying dues
of $100.00 regular member or $60.00 student member for a three-year term. Payment can be made following sending
the e-mail form and payment request to the ISBS Secretariat at info@stressandbehavior.com.
Please join our 2015-2017 ISBS conferences:

8th International Regional Neuroscience and Biological
Psychiatry Conference
"STRESS AND BEHAVIOR" (North America)
June 22-24, 2017, Miami, FL, USA
Psychiatry Conference "Stress and Behavior"
(Asia)
July 26-27, 2015, Kobe, Japan
3rd Caribbean Biomedical

Research Days CBRD-2016
January 16-18, 2016, Rodney Bay, St. Lucia
23rd International
Neuroscience and Biological
Psychiatry Conference "STRESS AND BEHAVIOR"
May 16-19, 2016, St. Petersburg, Russia

Psychiatry Conference
"STRESS AND BEHAVIOR" (North America)
June 22-24, 2016, New Orleans, LA, USA
www.stress-and-behavior.com
E-mail: info@stressandbehavior.com
41

Final Program and Proceedings - 5th International Regional &quot;Stress and Behavior&quot; Neuroscience and Biopsychiatry Conference (North America), June 22-24, 2015, Miami, FL, USA

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Final Program and Proceedings - 5th International Regional &quot;Stress and Behavior&quot; Neuroscience and Biopsychiatry Conference (North America), June 22-24, 2015, Miami, FL, USA

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International STRESS AND BEHAVIOR Society (ISBS)

ZENEREI Institute, USA

Stress and Behavior

Miami, FL, USA

ISBS OPENING AND WELCOMING. INDUCTION OF ISBS FELOWS

ISBS OPENING PLENARY LECTURE: UNDERSTANDING PTSD FROM

ISBS PRESIDENTIAL LECTURE: CROSS-DOMAIN PARSING OF GENETICS

ISBS SPECIAL FOCUS TALK: NORIBOGAINE MULTITARGET-DIRECTED

LUNCH BREAK (FREE TIME) AND EXHIBITION

ZUKOWSKA SYMPOSIUM ON STRESS NEUROSCIENCE

INTRODUCTION: PROFESSOR ZOFIA M ZUKOWSKA

BEHAVIORAL INHIBITION AND UNCERTAINTY ENHANCE CLASSICAL

NEUROTROPHIC FACTOR-1 PREVENTS STRESS-INDUCED DEPRESSION

National Institute of Child Health and Human Development (NICHHD), NIH,

PHARMACOLOGICAL STRESSOR ENHANCES MOTIVATION FOR FOOD

META-ANALYSIS OF THE EFFICACY AND SAFETY OF PRAZOSIN VERSUS

COFFEE BREAK AND EXHIBITION

THE IMPACT OF FLOTATION RESTRICTED ENVIRONMENTAL STIMULATION

EFFECT OF STRESS ASSOCIATED NEUROTRANSMITTERS ON THE

EFFECTS OF AIR TRAVEL STRESS ON THE CANINE MICROBIOME A PILOT

PRESENTATION: BIOSEB EB INSTRUMENTS: MJ Craddock, Bioseb In Vivo

Day 2. Tue, June 23, 2015

ISBS LECTURE: NEUROPSYCHOLOGICAL DEVELOPMENT IN INTERACTION

LAPIN SYMPOSIUM ON BIOLOGICAL PSYCHIATRY

INTRODUCTION: PROFESSOR IZYASLAV (SLAVA) P LAPIN

SELF-OBSERVATION, REFLECTION AND COPING WITH BRAIN CHEMISTRY

COFFEE BREAK AND EXHIBITION

ASSESSING GENDER DIFFERENCE IN AGGRESSION AMONG AUTISTIC

TELOMERASE DYSREGULATION IN THE HIPPOCAMPUS OF A RAT MODEL

PROTEIN BASED DIAGNOSTIC BIOMARKERS FOR EARLY DIAGNOSIS OF

LUNCH BREAK (FREE TIME) AND EXHIBITION

INTERACTIVE POSTER SESSION

DO SINGLE EXPERIENCES OF CHILDHOOD ABUSE INCREASE DEPRESSION AND

COMPARING PSYCHOLOGICAL MORBIDITY AMONG STUDENTS APPEARING FOR

IMPACT OF CHILDHOOD ADVERSITY AND VASOPRESSIN RECEPTOR 1A VARIATION

EFFECTS OF MOBILE TECHNOLOGY USE ON MOOD AND BODY MASS INDEX. CB

AMYGDALA INVOLVEMENT IN AVOIDANCE ACQUISITION IN A BEHAVIORALLY

EEG BIOFEEDBACK AND LAVENDER AROMATHERAPY IN MIGRAINE - A

EARLY LIFE STRESS AND THE BRAIN-GUT-MICROBIOTA AXIS: IMPACT OF N-3

THE USE OF MODERN TECHNOLOGY DURING TRAINING OF COGNITIVE PROCESSES.

ENRICHED ENVIRONMENT DECREASED ANXIETY-RELATED BEHAVIOR IN AN

INCREASED RISK OF SEXUALLY TRANSMITTED DISEASES AFTER BEREAVEMENT

THE CYSTIN/GLUTAMATE ANTIPORTER AS A POTENTIAL NOVEL TARGET TO

SEEKING SAFETY: A RANDOMIZED CLINICAL TRIAL. C Hansen, D Hien, Keiser

DEVELOPMENT AND IN VITRO CHARACTERIZATION OF NEUROMEDIN U ANALOGS. A

EVENT-RELATED POTENTIALS FROM PATIENTS WITH MAJOR DEPRESSIVE

COFFEE BREAK AND EXHIBITION

THE IMPACT OF MATERNAL TRAUMA HISTORY ON PEDIATRIC ASTHMA CONTROL. M

PHARMACOGENETIC TESTING SHOWS UTILITY FOR PSYCHIATRIC PATIENTS. H

SIMULTANEOUS EXPOSURE TO CHRONIC MILD STRESS AND ENRICHED DIETS

IMPLICATIONS OF HEALTH CARE COSTS ON CARDIOVASCULAR DISEASE

EXAMINING THE ROLE OF COMMUNICATION AND SOCIAL SUPPORT AS A MEDIATOR

Lyons, SR Webster, CA Feldman, B Nierenberg, Center for Psychological Studies, Nova

PERCEIVED DISCRIMINATION, ACCULTURATIVE STRESS, AND DEPRESSIVE

ULTRASONIC VOCALIZATION DURING EXPLORATION IN BOTH SAFE AND

Day 3. Wed, June 24, 2015

9th INTERNATIONAL ZEBRAFISH NEUROSCIENCE SYMPOSIUM

INTRODUCTION: THE INTERNATIONAL ZEBRAFISH NEUROSCIENCE RESEARCH

ZNRC LECTURE: ZEBRAFISH BIOLOGICAL PSYCHIATRY. DJ Echevarria,

ZNRC PRESENTATION: FROM A TO Z: ACTIVITY ANALYSIS AND DETAILED

ZNRC LECTURE: MODELING ACUTE AND CHRONIC STRESS IN ADULT

COFFEE BREAK AND EXHIBITION

ZNRC PRESENTATION: VIEWPOINT. P-A Fel, ViewPoint, Montreal, Canada

ASSESSING THE EFFECTS OF LIGHT DARK MANIPULATION AND CAFFEINE

Final Program and Proceedings - 5th International Regional "Stress and Behavior" Neuroscience and Biopsychiatry Conference (North America), June 22-24, 2015, Miami, FL, USA

Final Program and Proceedings - 5th International Regional "Stress and Behavior" Neuroscience and Biopsychiatry Conference (North America), June 22-24, 2015, Miami, FL, USA