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304-R1
1. Scope
(a) Column
equivalent.
Injection volume, 20 l.
4. Reagents
as an internal standard.
boronic
acid
(available
as
3. Apparatus
(b) Methyl
ionization detector.
nonadecanate
(internal
standard
substance)
(a) Column
2 m 3 mm (i.d.) glass column, packed with
a solid phase carrier (e.g., Chromosorb WAW,
DMCS, 100 120 meshes) coated with silicone
OV-225 as the liquid phase, or its equivalent.
(b) Operating parameters
(c)
(d) Sorbitol
5. Procedure
5.1. Determination of Moisture Content
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JCAM No.304-R1
mg per 1 hour drying period. For solid samples,
W0 W1
%, moisture =
100
W0
%, sorbitol =
AD
BC
100
(100 M)
100
of methyl nonadecanate)
D: The peak area ratio of sorbitol
derivative to methyl nonadecanate,
based on a gas chromatogram for the
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JCAM No.304-R1
Reference 1
B. HPLC Method
B.1. Preparation
of
Propylene
Glycol
(Internal
Standard) Solution
with water.
water.
the
sorbitol
standard
solutions
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constructed in B.5.
Calculate the sorbitol content in the test
sample using the following formula (Round to the
JCAM No.304-R1
first decimal place.).
(Wx /Ws ) Ms
S 1000
100
100 W
100
calculated
based
on
the
calibration curve
Ms:
S:
W:
Reference 2
6. References
(1) The United State Pharmacopeia 18ed.(1970)
(2) JAPAN'S SPECIFICATIONS AND STANDARDS
FOR FOOD ADDITIVES, the Seventh Edition
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