1/5/201

Maint
aining
Cell
Integri
ty

Dr. Vladimir
Sytnyk
Senior
lecturer,
Head,
Molecular
Neurobiolog
y Laboratory

School of
Biotechnolog
y and
Biomolecular
Sciences

Room 114
Samuels
Bldg.,

v.sytnyk@un
sw.edu.au

cytoskeleton
Endoplasmic
reticulum

mitochondria

Nucleus

Golgi
apparatus

Organelles,
Proteins,
Nutrients,

MEMBRANE

Energy (ATP),

..

Have to stay
inside of the
cell

Overvi
ew of
Todays
Lectur
e

Membrane
s

Compositio
n

Structure
and
Properties

Fluid Mosaic
Model

Fluidity and
Sidedness

Role of Lipids
in Maintaining
Integrity

Role of
Proteins in
Maintaining
Integrity
Membrane
permeability

Membr
ane
Models

phospholipid

bilayer with

hydrophobic

zone

different

organisation

of membrane

proteins

Fluid Mosaic
Model

DavsonDanielli
Model

(Singer &
Nicholson, 1972)

(1935)

Sidedn
ess
The plasma
membrane
has distinct
cytoplasmic
and
extracellular
faces.

The
extracellular
face is
topologically
equivalent to
the inside
face of the
ER, Golgi,
lysosome and
vesicle
membranes.

Proteins and
lipids do NOT
easily flip
from one side
of the

membrane to
the other.

1/5/2015

Members of the membrane

Members of the membrane

1. Lipids
Hydrophilic

2. Proteins

Peripheral

Hydrophobic

Integral

Transmembrane:

Proteins that span

Cholesterol

the membrane but

(025%oftotallipid)

have different

domains on each

side of the

membrane

Phospholipids

cell
human
after 1 hour

Fluid Mosaic Model

cell

Fluidity refers to the rapid movement of lipids

and proteins laterally, in the plane of the

membrane

hybrid cell

proteins are mixed

mouse

Rapid movement of lipids and proteins laterally, in the plane of

the membrane can also be visualised using microscopy
protein

Fluorescent probe

Source: Sytnyks Molecular Neurobiology Lab

Integral protein tagged with

GFP green fluorescent protein

Rapid movement of lipids and proteins laterally, in the plane of

the membrane can also be visualised using FRAP fluorescence recovery after photo- bleaching

of lipids and

using

laterally, in the

1/5/201
5
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t
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R
O
p
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1
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a
p
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i
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o
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it
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g
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at
er
all
y,
in
th
e

u
si
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o
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la

of

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e

br

ca
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al

so
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e
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se

in

us
g

r
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or
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nc

re

a
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e
co
ve
ry
af
te
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ot
obl
e
ac
hi
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g

O
p
t
i
o
n
2
:
f
l
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c
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T
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d
pr
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m
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sit
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pr
ot
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ed
wit
h

I
n
t
e
g
r
a
l

GF
P
gr
ee
n
flu
or
es
ce
nt
pr
ot
ei
n

Rapid
movement
of lipids and
proteins
laterally, in
the plane of
the
membrane
can also be
visualised
using FRAP
fluorescenc
e recovery
after photobleaching

Option 2:
fluorescenc
e recovers
with time

This means
that non-

photobleac
hed
proteins
move to the
photobleac
hed site

Integral
protein tagged
with
GFP green
fluorescent
protein

Source:
Sytnyks
Molecular
Neurobiology
Lab

C
e
l
l
r
e
c
o
g
n
i
t
i

1
/
5
/
2
0
1
5

M
e
m
b
e
r
s
o
f
t
h
e
m
e
m
b
r
a
n
e

3
.
C
a
r
b
o
h
y
d
r
a
t

d
H
2

O
w
i
l
l
a
l
s
o
c
r
o
s
s
m
e
m
b
r
a
n
e
s
b
y
d
i
f
f
u
s
i
o
n
.

I
o
n
i
s
e
d
,
p
o
l
a
r
a

i
s
i
s
i
m
p
o
r
t
a
n
t
f
o
r
t
h
e
m
a
i
n
t
e
n
a
n
c
e
o
f
c
e
l
l
i
n
t
e
g
r
i
t
y
!