Introducing Non-surgical Sterilization

Additional Considerations in Evaluations

The How,Why & When of Non-surgical Sterilization

PETER HOWE

BVSC(HONS) MSCMED PHD MANZCVS

HOW?

1. Vaccination to induce an immune response to endogenous

hormones and other key proteins
GnRH - BSA
GnRH - HSA
GnRH - KLH
GnRH LTB

Chemically conjugated
Antigenic but poorly Immunogenic
NEED ADJUVANTS - Freunds Complete and Incomplete
Chemically conjugated
Active when administered parenterally and orally
NEEDS ADJUVANTS - Freunds Complete and Incomplete
Patented 1987

GnRH Vitamin B12

Chemically conjugated
Orally active
Patented 1987

ALL VERY EXPENSIVE TO PRODUCE

1. Vaccination to induce an immune response to

endogenous hormones and other key proteins ( cont)

LTB- GnRH-GnRH-1/2(GnRH)

Recombinant DNA product

Very antigenic and immunogenic
Highly irritant to tissues LPS
Cheap to produce
Patented 1987
Used in fish

2. Using Agonists and Antagonists to GnRH

3. Toxins that destroy specific key cell lines

GnRH-Cis-platin
GnRH- Pokeweed / (D-Lys6) GnRH -Pokeweed
4-Vinylcyclohexene Diepoxide ( Chemspay)

4. Intra-testicular injection of Seminiferous tubule, Sertoli and

Leydig cell (mild) cytotoxins
Zinc Gluconate buffered with 0.2% Arginine
Calcium Chloride in alcohol ( Calchlor)
Lactic acid solutions
* Epididymal injections of Formol saline

4. Intra-testicular injection of Seminiferous tubule, Sertoli

and Leydig cell cytotoxins ( Cont)
Neutersol
Esterisol
Zeuterin

5. Novel delivery systems for GnRH agonists/antagonists and other

down-regulators of reproduction
Bacterial phages
Viruses - Adeno viruses

6. Other

Gene silencing - using micro RNAs

- target Kisspeptin ( Kiss1 gene) and
Neurokinin B
Gene therapy

Other ( cont)

Why?

When?

GnRH Antagonists
Yuendumu Dog Health and Population Control Program
2 week program
Treatment for ectoparasites and community education
37% reduction in dog population within 15 months
Mange became extremely rare and general health and
body condition vastly improved
Culture changed to seeing that having fewer dogs was
good
( Some surrendering and euthanasia occurred)

Dogs With No Names - Canada

Routinely use GnRH antagonists

* GnRH antagonists + Zeuterin

Zeuterin

It is not a matter of will non-surgical

sterilization become commonly used ,
but when. Will we be ready when it
does?