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KMA 9301
16S
Cultivation
16S
Cultivation
Fig. 1. Method
Sample
Tabel 1 shows the bacteria that where assumed not to have a clinical
relation to either endocarditis or meningitis.
rDNA purification
Data
Cultivation
Sequencing
PCR
Purification
16S
Heart valves
Spinal fluids
Staphylococcus epidermidis
Staphylococcus warnerii
Staphylococcus haemolyticus
Staphylococcus saphrophyticus
Corynebacterium sp.
Propionebacterium acnes
Methylobacterium sp.
Heart valves
Spinal fluids
2
1
1
1
Discussion: In the present study we have shown that 16S rDNA identification is a rapid and sensitive method to detect and identify bacteria in
samples where cultivation is difficult. From the perspective of clinical relevance, 16S versus cultivation also showed that the specificity was improved
by using 16S. Therefore 16S PCR and sequencing enables faster targeted antibiotic therapy in a higher number of patients.