PROTOCOL FOR REQUESTING ANF COLLECTING SAMPLES FOR RENIN AND

ALDESTRONE FOR PUTRAJAYA HOSPITAL

Collection of satisfactory specimens for RENIN ilnd ALDOSTERONE analysIs requires special procedure to prevent changes In RENIN and
ALDOSTERONE concentration while and after the specimen is drawn

Please take note that these are special analysis that is reserved to be requested only by Endocrinologist and
supported with patient's clinical history
PROCEDURE FOR SPECIMEN COLLECTION

A) RENIN

1. Please record posture of patient during sampling

2. Collect blood into pre-chilled tubes with sodium EDTA as an anticoagulant. The blood samples must not be
Withdrawn using haeparine as on ontico aoulcnt, as it interferes with angiotensin / generation.
I\li:iJ

1~:ICc cold

and centrifuge in cold (using refrigerated centrifuge) at 2000 X g to recover the EDTr\

plasma )~r'-r:W" '";,\,1',['. par ticuiat o rr.an c: turbiditv, lipaemia, or erythrocyte debris may require clarification by filtration

0:

centr,LJ,f:otrol"' :)cfore

testing.
Transfer the EDTA plasma Into a properly labelled tube (bar-code label or manual labelled) With the following Information

.
,.

Requested test

Piltien~'s name

Identity card number

Date of collection

I,: - up the PER. PATH 301 form. Please Include the patient's clinical history to support diagnosis of the test.
Senci the EOTA plasma specimen in ice to laboratory Immediately. If samples is not immediately send. stored In deep frozen (-:)0' C or lower)

8) ALDOSTEROr--J E
Diuretics, anuhvpert ensrve drugs, cyclic progestogens, estrogens and licorice should be terminated for at lea), IWU vvei~K:,. ,,rIG :nferabiy four weeks prior to
testing Patients should be on a normal sodium diet for 2 - 4 weeks (appr oxirnat eiv 135 mEq or .3

i' .if

SOC:UIil per

day).

Serum or fOTA plasma may be use and the usual pr er autionsfor venipuncture should be observed. PiC2se specify the
patient's position durrng spc cirnen collection whether supine or upright .
.)

!I suoine sample should be drawn In the early morning before the subject arises (if feasible). If an uprrght sample IS Indicated, the subject

should be upright for ~ 2 hours prior to sampling .

.:;

Ce ntrif uge the samples to recover the serum or EOTA plasma.

Transfer the serum or EOTA plasma into a properly label tube (bar-code label or manual labelled) With the following

Requested test

Patient's name

c.

Identity card number

Date of collection

r,ller. up the PER. PATH 301 form Please Include the patient's clinical history to support diagnosis of the test
Send the serum or EOTA plasma specimen

~1

Ice to laboratory Immediately. If samples is not immediately send. store ill ceeD frazer (-~O' C or

lower) .

'Collection tube for Aldasterorrc has been change from Heparirinized tube to fDTA tube due to the reagent kit changing

~ S)~/Q~Llbu f?q: I1cJmoj! faXo-lrJ'~ fJm/a

(ctld,A,.

do.ft1u/u :s6/fYJ in0CfiL.afl(.;{r 6~mfd

.
V

(-.

Dr. AsrT1la\1 b: Ar'Ji

MMC Full Reglstr;J1lOn No. 2Sfir','

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