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journal homepage: www.elsevier.com/locate/procbio
Short communication
a r t i c l e
i n f o
Article history:
Received 4 September 2011
Received in revised form 5 January 2012
Accepted 14 February 2012
Available online 22 February 2012
Keywords:
Ionic liquid
1-hydroxyethyl-3-methylimidazolium
bis(triuoromethanesulfonyl)amide
Keratin
Chicken feathers
Separation
a b s t r a c t
Keratin was extracted from chicken feathers by using a hydrophobic ionic liquid (IL), 1-hydroxyethyl-3methylimidazolium bis(triuoromethanesulfonyl)amide ([HOEMIm][NTf2 ]). Extracted keratin has good
solubility in water while the ionic liquid is immiscible with water, and therefore the extracted keratin
could be easily separated from the reaction system by water. The effects of ionic liquid, NaHSO3 , reaction
temperature and time were investigated and extracting conditions were optimized. The maximum yield
of keratin was up to about 21% with mass ratio of feathers to NaHSO3 1:1 and mass ratio of feathers to
ionic liquid 1:40 at 80 C for 4 h. Moreover, there was no obvious loss in the yield after ionic liquid was
reused for ve batches under optimized conditions. In addition, the recovery of ionic liquid was about
95% each time. The results indicated that [HOEMIm][NTf2 ] was very efcient as catalyst and solvent for
dissolving feathers and could be easily recovered due to its hydrophobicity.
2012 Elsevier Ltd. All rights reserved.
1. Introduction
The poultry industry produces a great amount of waste feathers
each year, e.g. 1.8 million tons in US [1], which causes an environmentally difcult disposal problem. Therefore, from both an
economic and environmental point of view, it is quite desirable
to develop effective and protable process to use these resources.
However, feathers are mainly used as low nutritional value animal
feed. Currently, researchers have been searching for new applications of feathers and many publications and patents proposing
applications of feathers have been issued. Polyethylene-based composites can be prepared using keratin bers obtained from chicken
feathers [2]. Keratin bers from chicken feathers were used as a
short-ber reinforcement for a poly(methyl methacrylate) matrix
[3]. Feather keratin and polyurethane were combined to synthesize hybrid synthetic-natural membranes which could be applied
to separation process [4].
Keratin is the major component of feathers, which is a structural
protein characterized by a high cystine content and a signicant
amount of hydroxyl amino acids, especially serine (about 15%) [5,6].
It contains a range of noncovalent interactions (electrostatic forces,
hydrogen bonds, hydrophobic forces) and covalent interactions
(disulde bonds), which must be destroyed in terms of dissolution of feathers. Keratin is insoluble in polar solvents like water,
weak acids and bases, as well as in apolar solvents while it is active
because cystine can be reduced, oxidized and hydrolyzed [711].
Corresponding author. Tel.: +86 21 6425 2695; fax: +86 21 6425 2695.
E-mail address: caoxj@ecust.edu.cn (X.-J. Cao).
1359-5113/$ see front matter 2012 Elsevier Ltd. All rights reserved.
doi:10.1016/j.procbio.2012.02.013
897
Table 1
Water content of equilibrated and dried IL; solubility in water of IL.
Ionic liquid
[HOEMIm][NTf2 ]
4.890
0.453
7.941
a
b
Water equilibrated refers to IL that has been stored in contact with water, which incorporates vigorous agitation and mixing.
Dried IL is water equilibrated IL that has been dried at 70 C for 6 h on a vacuum line.
898
Fig. 1. (a) Effect of mass ratio of feathers and NaHSO3 on the extraction yield of keratin. Reaction conditions: mass ratio of feathers to ionic liquid 1:20, 500 l water, 80 C,
4 h. (b) Effect of mass ratio feathers and ionic liquid on the extraction yield of keratin. Reaction conditions: mass ratio of feathers to NaHSO3 1:1, 1.0 g NaHSO3 , 500 l water,
80 C, 4 h. (c) Effect of reaction temperature on the extraction yield of keratin. Reaction conditions: mass ratio of feathers to NaHSO3 1:1, mass ratio of feathers to ionic liquid
1:40, 500 l water, 4 h. (d) Effect of reaction time on the extraction yield of keratin. Reaction conditions: mass ratio of feathers to NaHSO3 1:1, mass ratio of feathers to ionic
liquid 1:40, 500 l water, 80 C.
Fig. 2. The reusability of ionic liquid for dissolving feathers. Reaction conditions:
mass ratio of feathers to NaHSO3 1:1, mass ratio of feathers to ionic liquid 1:40,
500 l water, 80 C, 4 h.
3.4. FTIR
The FTIR spectra of extracted keratin in the region
4004000 cm1 are given in Fig. 3, where the characteristic
absorption bands are mainly assigned to the peptide bonds
899
keratin were 10,240 and 10,000 respectively. This GPC data agrees
with published data that feather keratin consists of 96 amino acid
residues and has a molecular weight of 10,206 [6]. The polydispersion degree was at the level of 1.024, which shows that the
extracted keratin was uniform in its molecular weight. However,
the yield of extracted keratin was less 25%, probably because keratin was degraded to amino acids and small peptides and not
separated from reaction mixture completely.
4. Conclusions
The eco-friendly ionic liquid [HOEMIm][NTf2 ] could be a suitable solvent and catalyst for dissolving feathers. To our knowledge,
this should be the rst report concerning the dissolution of feathers
in a hydrophobic ionic liquid and an easy separation of extracted
keratin.
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Fig. 3. IR spectrum of extracted keratin.
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