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Pigments are chemical compounds which reflect only certain wavelengths of visible
light. This makes them appear "colorful". Flowers, corals, and even animal skin contain
pigments which give them their colors. More important than their reflection of light is the
ability of pigments to absorb certain wavelengths.
Because they interact with light to absorb only certain wavelengths, pigments are useful
to plants and other autotrophs-organisms which make their own food using
photosynthesis. In plants, algae, and Cyanobacteria, pigments are the means by which
the energy of sunlight is captured for photosynthesis. However, since each pigment
reacts with only a narrow range of the spectrum, there is usually a need to produce
several kinds of pigments, each of a different color, to capture more of the sun's energy.
NATURAL PIGMENTS:
Natural pigments are those pigments which are derived from animal, vegetable, and
mineral substances.
EXAMPLES
OF
COLOUR PIGMENT EC STRUCTURE
NATURAL No.
OCCURRENCE
Black Grapes
Cherries
Elderberries
Red Cabbage
Strawberries
Alfalfa Grass
Spinach Chlorophyllins
Carotenoids:
Annatto Yellow
Saffron Bixin/Norbixin ( ii )
E 160c
Lycopene
Apocarotenal
E 160d
Apocarotenal
Lutein E 160f
Canthaxanthin
E 161b
E 161g
Milk
Yeast
Carbonized Black Carbon Black E 153
Vegetable Material
Melanoidins Brown Melanoidins E 150a-d
(Caramel)
Anthocyanins:
• Anthocyanins are water soluble pigments.
• They are responsible for the attractive red, purple and blue colors of many
flowers, fruits and vegetables.
• They are sensitive to pH change, being reddest in strongly acidic conditions and
become bluer as the pH rises.
• Chemically anthocyanins are subdivided into the sugar-free anthocyanidine
aglycons and the anthocyanin glycosides.
• They are used as food additive with E number E163.
Structure:
• Over 500 different anthocyanins have been isolated from plants. They are all
based on a single basic core structure, the flavyllium ion.
• When anthocyanidins are coupled to sugars, anthocyanins are formed. As
sugars can be coupled at different places and many different sugars are present
in plants, it is clear that a very large range of anthocyanins can be formed.
• For example in strawberries the main anthocyanins are Cyanindin-3-glucoside
and Pelargonidin-3-glucoside, two relatively simple structures.
100 g food
aubergine (egg plant) 750
black currant 130-400
blackberry 83-326
Blueberry 25-497
Cherry 350-400
chokeberry 200-1000
cranberry 60-200
Elderberry 450
orange ~200
Radish 11-60
raspberry 10-60
red currant 80-420
red grape 30-750
red onions 7-21
red wine 24-35
Strawberry 15-35
Use:
• Anthocyanins are water soluble strong colours and have been used to colour
food since historical times. Extracts of berries have been used to colour drinks,
pastries and other foods.
• Grape peel (E163(i)), and black currant extract (E163(iii)) are the most widely
used anthocyanin mixtures in foods.
Disadvantages:
• Color is susceptible towards temperature, oxygen, UV-light and different co-
factors. Temperature may destroy the flavylium ion, and thus causes loss of
colour.
• Temperature causes Maillard reactions, in which the sugar residues in the
anthocyanins may be involved. Light may have a similar effect.
• Oxygen may destroy the anthocyanins, as do other oxidizing reagents, such as
peroxides and vitamin C.
• Many other components in plants and foods may interact with the anthocyanins &
either destroy, change or increase the color. Quinones in apples, for example,
enhance the degradation of anthocyanins, whereas the addition of sugar to
strawberries stabilizes the color.
Beetroot colors (betalains)
The colour of beetroots is caused by the colours, betanin and vulgaxantin. Beetroot
extract is used as a food colour and has E-number E162.
Structure:
Betalains are classified on their structure and divided into two groups; betacyanins and
betaxanthins, with red-purple and yellow colours respectively. More than 50 betalains
have been described.
In beetroot the main betacyanin is betanin and the main betaxanthins are vulgaxantin I
and vulgaxanthin II (fig 3). The latter only differ in one side group of the molecule.
Betanin
Use:
• Beetroot extract or –pulp has been used to colour food for many centuries, but
the beetroot itself always was an ingredient.
• The use of betalains as separate food colours date from the early 20th century,
when in the US pokeberry (Phytolacca Americana) juice was added to wine to
enhance the colour. Nowadays only beetroot extract has been approved as a
colour in most countries.
• All betalains are water soluble, which limits the use. Betalains are stable between
pH 3.5 and 7.0 which covers nearly all foods, with maximum colour stability at pH
5.5. Betanin is susceptible towards light and temperature, which limits the use to
fresh foods, foods packed under modified atmosphere, or foods that undergo no
heat treatment. It is mainly used in frozen products (ice cream, yoghurt).
• Dry Betanin is more stable and it is used as a colour in instant foods powdered
soft drinks. It is also stable in high sugar conditions and can thus be used in
candies and fruit gels and fillings.
Carmine is the name of the pigment; the actual colour is carminic acid (C22H20O13):
Carmine
Cochineal is one of the few natural and water-soluble colorants that resist degradation
with time. It is the most light- and heat-stable and oxidation-resistant of all the natural
colorants and is even more stable than some synthetic food colours.
Cochineal it is neither toxic nor known to be carcinogenic. However, the dye can induce
an anaphylactic-shock reaction in a small number of people, due to impurities in the
preparation, not due to the carminic acid.
Use:
• Cochineal is widely used as a food colouring agent.
• Cakes, cookies, beverages, jam, jelly, ice cream, sausages, pies, dried fish,
yogurt, cider, maraschino cherries and tomato products were brightened with it
as were chewing gum, pills and cough drops.
• It is also have its application in alcoholic beverages and processed meat
products.
Chlorophyll
• Chlorophyll is a green pigment found in most plants, algae, and certain bacteria.
As all leaves and thus all leafy vegetables contain chlorophyll, it is one of the
oldest and most widely consumed pigments in our diet. It has been in the human
diet forever, so it is considered as one of the safest food components.
• Chlorophyll plays an important role in plants photosynthesis, the mechanism by
which plants acquire energy.
• Purified chlorophyll is used as a food colour with E-number E140, the more
stable copper complexes of chlorophyll are number E141.
Structure:
Chemically chlorophyll is a mixture of several highly complex molecules, which consist
of a ring structure (the porphyrin structure) with a central magnesium ion, and a long
hydrophobic side chain.
Use:
• Due to its safety, chlorophyll can be added unlimitedly to nearly all foods.
• It is mainly used in sugar confectionery and dairy products.
• Chlorophyll-coloured products should be dry and not exposed to light, air or high
temperatures, In many cases the products are packed in dark packaging with a
modified atmosphere to prevent chlorophyll degradation.
Carotenoids
• Carotenoids are a class of natural pigments that is very widespread and it was
demonstrated that they occur in all the three domains of life, i.e. in the
eubacteria, the archea and in the eucarya.
• A rich source for carotenoids are the algae and more than 100 carotenoids have
been isolated and characterized from these organisms.
• For humans the most important source for carotenoids are plants, where often
the brilliant colours of the carotenoids are masked by chlorophyll, e.g. in green
leaves.
• The carotenoids are responsible for the beautiful colors of many fruits (pine-
apple, citrus fruits, tomatoes, paprika, rose hips) and flowers (Eschscholtzia,
Narcissus), as well as the colours of many birds (flamingo, cock of rock, ibis,
canary), insects (lady bird), and marine animals (crustaceans, salmon).
• Normally carotenoids occur in low concentrations, but this varies enormously
from one source to another.
• The total carotenoid production in nature has been estimated at about
100.000.000 tons a year.
• Recently it was demonstrated by the analysis of serum and human breast milk
that up to 50 dietary carotenoids from fruits and vegetables may be absorbed
and metabolised by humans.
Examples of carotenoids in common foods: Carrot
• In natural products the most common carotenoid is the yellow-orange pigment of
the carrot (Daucus carota), the ß,ß-carotene . It was isolated in crystalline form
as early as in 1831 by
Chives 2 Melon 2
Structure of Carotenoids:
Use:
• Carotenoids added as colorants to many manufactured foods, drinks and animal
feeds, either in the form of natural extracts (e.g. annatto) or as pure compounds
manufactured by chemical synthesis.
• The production of carotenoids by biotechnology is of increasing interest.
Carotenoids are essential to plants for photosynthesis, acting in light-harvesting
and, especially, in protection against destructive photo oxidation.
• The essential role of beta-carotene and others as the main dietary source of
vitamin A has been known for many years. More recently, protective effects of
carotenoids against serious disorders such as cancer, heart disease and
degenerative eye disease have been recognized, and have stimulated intensive
research into the role of carotenoids as antioxidants and as regulators of the
immune response system.
Curcumin (Turmeric)
Turmeric (Curcuma longa) is an important spice and colouring agent and has been used
for more than 2000 years.
Turmeric (Curcuma longa) is an important spice and colouring agent and has been used
for more than 2000 years.
The colour consists mainly of curcumin and is used as a food colour with E-number
E100(i), crude turmeric powder is E100(ii).
Structure:
The main compounds in turmeric are curcumin and its derivatives demethoxycurcumin
and bis-demethoxycurcumin. The latter differ from curcumin in that the CH3-groups are
removed. The ratio between the three pigments differs between preparations, but
curcumin is present in the highest concentration and bis-demethoxycurcumin in the
lowest concentratin.
Structure of Curcumin
Use:
• Curcumin is not soluble in water, but dissolves in oils and alcohol. It is quite
stable and gives a bright orange-yellow colour to products. In Asian cuisine
turmeric is mainly used as a spice, whereas in Western countries the main use is
as a colouring agent
• Curcumin is not stable under alkaline conditions above 7.5, which do not occur in
many foods. Curcumin is light sensitive but stable at temperatures used in food
processing. Light sensitivity is decreased by the addition of aluminum ions, which
form a complex with curcumin and thus protect degradation by light.
• Turmeric and curcumin are widely used in foods and cosmetics. Applications
include canned beverages, baked products, dairy products, ice cream, yoghurts,
yellow cakes, biscuits, popcorn-colour, sweets, cake icings, cereals, sauces etc.
Flavonoids
The flavonoids are polyphenolic compounds ,with structure similar to those of
anthocyanins and also occur as glycosides.Flavonoids differ from anthocyaninin having
a carbonyl group in position 4.These include the groups of
compounds,flavones,flavonoids,flavonols,flavanones(and
isoflavanones),chalcones,aurones and biflavanyls.
Flavone Flavonol
Flavanone
Good sources of flavonoids include all citrus fruits, berries, ginkgo biloba, onions,
parsley, pulses,tea (especially white and green tea), red wine, seabuckthorn, and dark
chocolate (with a cocoa content of seventy percent or greater).
Green tea flavonoids are potent antioxidant compounds, thought to reduce incidence of
cancer and heart disease.
Flavonoids exist naturally in cacao, but because they can be bitter, they are often
removed from chocolate, even the dark variety.
Riboflavin
Riboflavin, Vitamin B2, is used for fortification and colouring. It is water soluble, heat
stable and is used in dairy products, cereals and dessert mixes
Carbon and metals
Vegetable carbon black is a heat and light insoluble pigment, used primarily in sugar
confectionery. Metals, such as gold, silver and aluminum are used for surface colouring,
mainly in confectionary.