RESULTS AND DISCUSSION

Volume of Eluate

Color of Component

In this experiment, two types of chromatography

(Drops)

were used for the purpose of separation and

1
2
3

purity determination. Column chromatography,

These eluates were then used as individual

which runs a solvent that moves down slowly

samples for spotting the TLC plates which were

through a glass column with packed adsorbent,

then put inside the developing chamber for

was used to separate the colored components of

completing

malunggay leaves. On the other hand, thin layer

Retention

chromatography (TLC) which utilizes a stationary

measuring the distance of each component from

phase composed of a thin layer of adsorbent

the origin and by dividing this to the solvent

material on a glass plate or plastic sheet, was

front, represented by the formula:

based on solid-liquid chromatographic methods

used

to

separate

the

components

of

each

on

the

retention

values

(Rf

values)

obtained.
Starting

with

separating
malunggay

the

column

chromatography

for

colored

components

the

leaves,

hexane-acetone

of

was

with the same reagent, to be added next to

perform the chromatography process.
As the pure extract slowly moved down the
column, 3.0 mL each of the solvents, specifically
hexane-acetone, acetone, and acetone-methanol
respectively, were added continously at the same
time while collecting different colors of eluates in
separate test tubes. For this, four differentcolored eluates were collected, of which two were
mixed to provide three distinct-colored eluates,
particularly, pale yellow, green, and yellow green.
The following table presents the data collected
under column chromatography.
Table 1. Characteristic Color and Volume of
Collected Eluate (Column Chromatography)

were

chromatography.

then

determined

by

Rf Value
Distance of

packed adsorbent without dissolving the solid.

pure extract of malunggay leaves was prepared

factors

layer

Component from Origin (X) with Determined

be used as it run through the glass column with

column to prevent it from running dry, as the

thin

Table 2. Characteristic Color and Distance of

determined to be the suitable solvent system to

This reagent was continuously added up on the

the

Distance of sample
Distance of solvent
Rf = origin origin

collected eluate to overall determine their purity

based

Pale yellow
Green
Yellow Green

1
2
3

Color of

component

Component

from origin (X)

Pale Yellow
Green
Yellow Green

in cm
4.89 cm
4.79 cm
0.42 cm

(attach

explanation

why

Rf Value

0.75
0.74
0.42

retention

factor

is

important for purity determination or how thin

layer chromatograhy is related to the purity of
the samplenow this explanation correlates with
that

of

the

chromatogram

reuslts
as

seen

obtained
from

from

the

the

distance

between each eluate, and as shown from the

actual chromatogram presented below etc etc)

Figure

1.
Figure 2. Dried Chromatogram with
Markings

Chromatogram under UV light

In conclusion